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Quantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables.
The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses.
The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction.
Our used lab equipment is fully refurbished, guaranteed to work up to manufacturers specifications and are delivered to our customers in as close to new condition as possible. Typical turnaround for refurbishing of used lab equipment is 2-4 weeks. They also come with our standard 6 Month parts and labor warranty. Prices do not include S/H unless otherwise indicated. Pictures enclosed and the actual unit may vary unless specified.