We offer an assortment of used and refurbished Thermal Cyclers ranging from Real Time PCR to qPCR, and Gradient Thermal Cyclers to non-Gradient Thermal Cyclers. Some of our popular manufacturers include ABI, MJ-Research, Bio-Rad, Agilent / Stratagene, and Eppendorf.
The MJ Research PTC-100 is a robust thermocycler that provides solutions for various temperature configuration needs. This instrument comes in versions that use of 96-well or 60-well microplates, as well as a heated lid for oil-free operation. The Hot Bonnet heated lid fitted in each unit has a thumbwheel that allows for the convenient adjustment of height and pressure. The modification feature ensures the tight sealing of reaction vessels ¬and microplates ¬ during oil-free thermal cycling.
96 Well Block
The Precise Peltier-effect heat pumps of PTC-100 thermocyclers were crafted to withstand repeated cycling. Any temperature sequence can be cycled up to 10,000 times. The NIST-traceable calibration feature of this instrument allows for the indefinite storing of material at any temperature. As a result, incubation rates are highly accurate. This calibration feature is also responsible for the refrigeration of results after unsupervised runs.
Data storage is secured and hassle-free with MJ Research PTC-100 thermocyclers. Memory stores approximately 320 programs, and the cycler comes with 14 pre-programmed protocols. Any sequence of temperatures can be cycled up to 10,000 times. Ramping rates less than maximum can also be specified. Data which were not properly stored due to power interruptions are safe for up to twelve hours with the PTC-100's power-failure protection feature.
25 well MiniCycler
The MJ Research PTC-150 MiniCycler is a reliable easy-to-use thermal cycler, designed by field experts. Users can take advantage of the PTC 150's easy-to-read programming interface. The MJ Research thermal cycler can also perform programming tasks as quickly and hassle-free as possible. Instrument set-up and transportation can be done with ease as well with the MiniCycler's space-saving design. Other notable capabilities of the PTC-150 are the Instant Incubate feature for continuous-temperature incubations and oil-free cycling as a result of using a Hot Bonnet heated lid.
The MJ Research PTC-225 is an innovative thermal cycling system capable of simultaneously running four individual sample blocks without compromising accuracy and performance. This reliable Tetrad system is ideally suited for labs with high throughput PCR applications. Additionally, the PTC-225 Thermal Cycler features a "hot swappable" block, as well as gradient and non-gradient controlled base options. Notable features of the PTC-225 include a high-density display, an on-board point-and-click software navigation, and real-time monitoring of cycling temperature. The Tetrad PTC-225 Thermal Cycler system includes the MJ Research PTC-225 thermal cycler base and four alpha blocks (96, 384, 30x30, 60x60 well).
The DNA Thermal Cycler 480 automates the polymerase chain reaction (PCR) technique for amplifying DNA.
The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. Offering unmatched flexibility and reliability, the systems are ideal for a wide variety of applications and chemistries (including but not limited to: gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis and chromatin immune-precipitation studies.
Highly reproducible results are the product of the Mx3005P’s single light source, single detector precision optic scanning design, providing uniform excitation and detection, coupled with the trusted Peltier-based thermal system, which ensures uniform ramping and thermal accuracy.
Quantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables.
The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses.
The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction.