Applied Biosystems 7900HT Real-Time PCR

Cambridge Scientific ID: 24928 Category: Tag:


The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability.

  • Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate
  • Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction
  • Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences
  • 96- or 384-well plate compatibility (including the TaqMan® Low Density Array)
  • Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security
  • Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab’s productivity
  • Proven assay development guidelines save time and money


  • Calibrated Dye: SYBR® Green I, ROX™, NED™, JOE™, TAMRA™, VIC™, FAM™, TET™
  • Capacity: Up to 84 x 384-well plates with optional automation accessory
  • Detection Method: SYBR, Primer-Probe Detection
  • Dimensions: 72 cm (W) x 84 cm (D) x 64 cm (H) (with drawer open)
  • High-throughput Compatibility: High-throughput Compatible, Multiplexing
  • Optics: Extended-life 488 nm argon-ion laser excitation source, No filters (CCD acts as spectrograph with continuous detection 500 -650 nm)
  • Passive Reference Dye: ROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)
  • Reaction Speed: Fast 
  • Reaction Volume Range: 5-20 µl
  • Run Time: 52 minutes (384-well in fast mode), <2 hrs (384-well in standard mode)
  • Sample Ramp Rate: Fast mode: ± 3.0°C⁄sec, Standard mode: ± 1.6°C⁄sec
  • Temperature Accuracy: ±0.25°C of display temperature
  • Temperature Range (Metric): 4-100°C
  • Temperature Uniformity: ±0.5°C
  • Thermal Cycling System: Peltier-Based System
  • Throughput: >5,000 real-time PCR rxns per day (384-well block and automation accessory), >10,000 endpoint PCR rxns per hour (384-well block and automation accessory)
  • Weight: 114 kg (250 lbs) with automation accessory, 82 kg (180 lbs) without automation accessory
  • Block Format: Interchangeable
  • Dynamic Range: Up to 9 logs of linear dynamic range
  • Sensitivity: Down to 10 copies

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