BioTek Synergy HTX Microplate Reader

Cambridge Scientific ID: 25614 Category: Tag:

Description

Includes Computer Loaded w/ Gen 5 Software

 

Synergy HTX Multi-Mode Microplate Reader is a compact, affordable system for 6- to 384-well microplates and Take3 Micro-Volume Plates. Absorbance, fluorescence, luminescence and AlphaScreen/ AlphaLISA measurements are all made using a unique dual-optics design that provides superior performance. The absorbance detection optics uses a xenon flash lamp and monochromator for filter-free wavelength selection from 200 to 999 nm in 1 nm increments. Fluorescence determinations are made using a tungsten halogen lamp with interference filters in conjunction with a PMT detector for maximum sensitivity. Synergy HTX also features BioTek’s unique 4-Zone incubation to 50 ºC, available dual reagent injector module, plus linear and orbital shaking to meet a wide variety of assay requirements. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 Data Analysis Software for data collection, analysis, exporting and reporting. For increased workflow automation and throughput, BioTek’s BioStack can be easily connected to Synergy HTX to automatically process up to 50 microplates at a time. For convenience, versatility and affordability, Synergy HTX is the ideal multi-mode microplate reader.

 

General

  • Detection modes:      
    • UV-Vis absorbance
    • Fluorescence intensity
    • Luminescence
    • Alpha
  • Read methods: End point, kinetic, spectral scanning and well area scanning
  • Microplate types: 6- to 384-well plates
  • Other labware supported: PCR plates, Petri and cell culture dishes, Take3 Micro-Volume Plates
  • Temperature control:
    • 4-Zone incubation to 50 °C with Condensation Control
    • +0.2 °C at 37 °C
  • Shaking: Linear, orbital
  • Software: Gen5 Data Analysis Software
  • Automation: BioStack and 3rd party automation compatible

Absorbance

  • Light source: Xenon flash
  • Detector: photodiode
  • Wavelength selection: monochromator
  • Wavelength range :200 – 999 nm, 1 nm increments
  • Monochromator bandwidth:4 nm
  • Dynamic range: 0 – 4.0 OD
  • Resolution:0001 OD
  • Pathlength correction: yes
  • Monochromator wavelength accuracy: +2 nm
  • Monochromator wavelength repeatability: +0.2 nm
  • OD accuracy:
    • <1% at 2.0 OD
    • <3% at 3.0 OD
  • OD linearity: <1% from 0 to 3.0 OD
  • OD repeatability: <0.5% at 2.0 OD
  • Stray light:03% at 230 nm
  • Reading speed (kinetic):
    • 96 wells: 14 seconds
    • 384 wells: 26 seconds

Fluorescence Intensity

  • Light source:
    • Tungsten halogen
    • Xenon flash (option)
  • Detector: PMT
  • Wavelength selection: Filters
  • Wavelength range: 300 – 700 nm (200 – 850 nm option)
  • Dynamic range: >6 decades
  • Sensitivity: Top and Bottom: Fluorescein 5 pM (1 fmol/well 96-well plate)
  • Reading speed (kinetic):
    • 96 wells: 31 seconds
    • 384 wells: 80 seconds

Luminescence

  • Wavelength range: 300 – 700 nm
  • Dynamic range: >6 decades
  • Sensitivity:
    • 10 amol ATP (flash) – Lum and Abs / Lum configurations
    • 30 amol ATP (flash) – Multi-mode configurations

Alpha Detection

  • Light source: Tungsten halogen
  • Detector: PMT
  • Wavelength selection: Filters
  • Sensitivity: 300 amol of biotinylated-LCK-P peptide, 25 µL/ well in 384-well plate
  • Read speed: 2 minute (96-well plate)

Reagent Dispensers

  • Supported detection modes: All modes
  • Number: 2 syringe pumps
  • Supported labware: 6- to 384-well plates
  • Dead volume:1 mL with back flush
  • Dispense volume: 5-1000 µL in 1 nm increments
  • Dispense accuracy: +1 µL or 2%
  • Dispense precision: <2% at 50 – 200 µL

Physical Characteristics

  • Connectivity: 1 USB, 1 RS232 for external PC control
  • Power: 100-240 VAC, 50/60 Hz
  • Dimensions:16″W x 15″D x 10″H (40.6 x 38 x 25.4 cm)
  • Weight: 40 lbs (18 kg)

 

General

  • BioCell capacity: 2
  • 2 µL sample capacity: Take3
  • Cuvette capacity: Take3: 1
  • Compatible readers: Take3: Epoch, Epoch 2, Synergy 2, Synergy H1, Synergy Neo 2, Synergy HTX, Cytation 5 and Cytation 3
  • Optical pathlength: 0.5 mm (nominal)
  • Detection limit: 2 ng/µL dsDNA
  • Predefined applications
  • Nucleic acid and protein quantification
  • In-situ fluorometric protein quantification
  • 260/280 and 260/230 protein purity measurements
  • Very low volume in-situ BCA assay
  • Spectral scan of single or multiple low volume samples

Contact Us

Thank you for your interest in the BioTek Synergy HTX Microplate Reader (SKU:25614) To speak with a representative, please fill out the contact form below or call us directly.

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