Description
High Performance, Label-free Kinetics Characterization
The Octet R2 System makes high-quality interaction analysis affordable and flexible. Priced for startup academic and biotech labs and low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The 2-channel Octet R2 system offers an advanced, fluidics-free approach to interaction analysis, with a wide variety of off -the-shelf Dip and Read biosensors for binding kinetics and quantitation analysis. Octet R2 provides a low-cost entry point for labs with lower throughput needs, while providing the flexibility to field-upgrade to 4 or 8 channels anytime the need arises.
Specifications:
- Detection Technology: Bio-Layer Interferometry (BLI) based on fiber optic biosensors
- Biosensor Type: Disposable, single-use fiber optic biosensors with optional resume by regeneration and/or re-racking in the biosensor tray
- Data Presentation:
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- Graphs displaying real-time kinetic binding sensorgrams, fitted result plots, and residuals of fits
- Concentration data analysis including calibration curves and output of tabulated concentration data
- Epitope binning and cross-blocking matrices and trace overlays
- Tabulated kinetic data
- Sample Type: Proteins, antibodies, peptides, DNA, RNA, liposomes, viruses and virus-like particles in various media including serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants and crude cell lysates.
- Number of Spectrometers: 2
- Maximum Simultaneous Reads: 2
- Data Collection Rate: 2, 5 or 10 Hz
- Sample Position and Format: 1 standard 96-well, black, flat-bottom microplate
- Sample Volume: 180-220 µL/well, Nondestructive testing
- Orbital Flow Capacity: Static or 400-1500 rpm
- Analysis Temperature Range: 15°C to 40°C, in 1°C increments
Quantitation and Kinetics
- Throughput: Up to 2 assays in parallel
- Molecular Weight Detection: >150 Da
- Analysis Time per Sample: hlgG quantitation in 2 minutes for 2 samples real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
- Affinity Constant: 1 mM- 10 pM
- Quantitation Range:
- 0.5 µg/mL to 2000 µg/mL (Human IgG and Protein A Biosensor)
- Protein – and assay – dependent
- Baseline Noise (RMS): <3.0 pm
- Baseline Drift: < 0.1 nm/hour
- Quantitation Precision: CV < 10 %
Instrument
- Weight: 72 lb (32.7 Kg)
- Dimensions (H x W x D): 19.5 in x 22 in x 18.2 in / (49 cm x 56 cm x 46 cm)