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Applied Biosystems StepOne Plus Real-Time qPCR System

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Applied Biosystems StepOne Plus  Real-Time qPCR System

Applied Biosystems StepOne Plus Real-Time qPCR System

Cambridge Scientific ID #: 22659

Includes Computer loaded w/ Step One Software


The StepOnePlus Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Features of the StepOnePlus Real-Time PCR System include:

  • Advanced software and instrumentation for performing a wide array of genomic assays
  • Sensitive 4-color optical LED recording system
  • Intuitive and robust software perfect for both first-time and advanced users
  • Simple and flexible instrument set-up and usage



  • Capacity: 1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes
  • Detection Method: Primer-Probe Detection, SYBR
  • Dimensions: 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
  • Dynamic Range: Linear Dynamic Range greater than 9 log units (detection)
  • Format: 0.1 ml tubes, 8-tube strips, 96-well plate
  • Green Features: Energy efficient, Fewer resources used, Sustainable disposal
  • High Throughput Compatibility: Multiplexing
  • Optics: 4 emission filters, Blue LED excitation source, photodiode
  • Passive Reference Dye: No ROX, ROX (Pre-mixed), ROX (Separate Tube)
  • Peak Block Ramp Rate: 4.6°C⁄sec
  • Reaction Speed: Fast, Standard
  • Reaction Volume Range: 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
  • Run Time: <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
  • Sample Ramp Rate: Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
  • Temperature Accuracy: 0.25°C (35 to 95°C) of display temperature
  • Temperature Range (Metric): 4-100°C
  • Temperature Uniformity: 0.25°C (35 to 95°C) of setpoint/display temperature
  • Thermal Cycling System: Peltier-Based System
  • Weight: 24 kg (53 lbs.)
In Stock Worldwide Shipping Call: 1-877-Used-Lab

Our used lab equipment is fully refurbished, guaranteed to work up to manufacturers specifications and are delivered to our customers in as close to new condition as possible. Typical turnaround for refurbishing of used lab equipment is 2-4 weeks. They also come with our standard 6 Month parts and labor warranty. Prices do not include S/H unless otherwise indicated. Pictures enclosed and the actual unit may vary unless specified.


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