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Dionex Ultimate 3000 FLD-3400RS HPLC Fluorescence Detector

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Dionex Ultimate 3000 FLD-3400RS HPLC Fluorescence Detector

Dionex Ultimate 3000 FLD-3400RS HPLC Fluorescence Detector

Cambridge Scientific ID #: 15165

The Thermo Scientific Dionex UltiMate 3000 FLD-3000 Fluorescence Detector series provides high-sensitivity fluorescence detection for UltiMate 3000 HPLC systems. It is available in rapid separation (200 Hz) and standard (100 Hz) versions. The optics of the FLD-3000 Fluorescence Detector series provide maximum stray light suppression for unrivaled detection sensitivity. Innovative technologies supply various tools for individual method development, such as 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition. Operated with Thermo Scientific Dionex Chromeleon Chromatography Data System software, these detectors provide automated qualification, performance optimization, and instrument wellness monitoring for ease of use, maximum uptime, and the highest degree of regulatory compliance.

  • Data collection at up to 200 Hz for optimal support of even the shortest UHPLC separations
  • Lowest limits of detection with a Raman S/N: > 550 ASTM (> 2100 using dark signal as noise reference)
  • Unsurpassed reproducibility with active flow cell temperature control for stable fluorophore activity independent from changes in ambient temperature
  • Long-life xenon flash lamp for highest sensitivity and long-term operation without the need for frequent lamp changing
  • Optional second photomultiplier for unique Dual-PMT operation offering an extended wavelength range up to 900 nm without sacrificing sensitivity in the standard wavelength range
  • 2D or 3D excitation, emission, or synchro scans provide the highest degree in flexibility for method development or routine sample characterization
  • Innovative variable emission filter for real-time, compound-related sensitivity optimization (FLD-3400RS only)
  • Large front panel display for easy monitoring of detector status
  • Flow cell syringe injection kit as a cost-effective solution to perform off-line measurements
  • Optimized flow cell design ideal for trace analysis in combination with narrow-bore UHPLC columns and conventional HPLC columns
  • Full regulatory compliance with automated qualification period monitoring and automatic tracking of flow cells



  • Light Source: Xenon Flash Lamp
  • Lamp Pulse Frequency:
    • HighPower (~300 Hz)
    • Standard (~100 Hz)
    • LongLife (~20 Hz)
  • Data Collection Rate:
    • Single wavelength: up to 200 Hz (FLD-3400RS)
    • Multiple wavelength: up to 4 Hz (FLD-3400RS)
    • Single wavelength: up to 100 Hz (FLD-3100)
  • Spectrum Scanning Modes: 2D or 3D excitation, emission or synchro scans (3D scans under Chromeleon 7.1 control)
  • Wavelength Range:   
    • Variant  Ex Min   Ex Max  Em Min Em Max
    • 3100     200       630       265       650
    • 3400RS 200       630       220       650
    • 3100 with Dual-PMT       200       880       265       900
    • 3400RS with Dual-PMT   200       880       220       900
  • Spectral Bandwidth:
    • Excitation: 20 nm
    • Emission: 20 nm
  • Emission Filter:          Variable, 5 positions (FLD-3400RS) Fixed, 280 nm (FLD-3100)
  • of Fluorescence Channels: Up to 4 (FLD-3400RS) 1 (FLD-3100)
  • Excitation/Emission Wavelength Switching Time: < 250 ms
  • Wavelength Accuracy: ±2 nm
  • Wavelength Repeatability: ±0.2 nm
  • Sensitivity: Raman S/N: > 550 ASTM over the entire lifetime of the lamp (> 2100 using dark signal as noise reference)
  • Flow Cell Thermostatting: Ambient + 10 °C to 50 °C
  • Flow Cell:
    • Analytical flow cell volume: 8 μL
    • 20 bar (290 psi) maximum cell pressure
    • Micro flow cell volume: 2 μL
    • 40 bar (580 psi) maximum cell pressure
  • PC Connection:           All functions controllable through USB
  • GLP Features: Automatic Equipment Qualification (AutoQ), System Wellness, and Qualification Monitoring with Chromeleon, cell ID chip
  • Dimensions (h × w × d):
    • 16 × 42 × 51 cm
    • (6.3 × 16.5 × 20 in.)
  • Weight: 18 kg
In Stock Worldwide Shipping Call: 1-877-Used-Lab

Our used lab equipment is fully refurbished, guaranteed to work up to manufacturers specifications and are delivered to our customers in as close to new condition as possible. Typical turnaround for refurbishing of used lab equipment is 2-4 weeks. They also come with our standard 6 Month parts and labor warranty. Prices do not include S/H unless otherwise indicated. Pictures enclosed and the actual unit may vary unless specified.


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