Used Lab Equipment

Multiskan Spectrum makes getting results easier, replacing separate spectrophotometers and microplate reading instruments by providing both functions in one convenient, compact instrument.  The Multiskan Spectrum is truly two instruments in one, combining both cuvette reading capabilities and advanced microplate reading in one convenient, compact instrument.  You can easily take quick absorbance measurements in a cuvette between the cycles of a microplate enzyme kinetics experiment. 

For maximum flexibility, Multiskan Spectrum accepts all microplate for mats from 6- to 384-wells, as well as cuvettes from standard to ultra micro capacities in glass, plastic, or quartz. You can take multiple photometric readings per well on large format plates, or single measure ments requiring just 30 μl in 384-well plates.

Scanning range of 200 – 1000 nm.  The built-in monochromator makes it simple to select any wavelength from 200 nm to 1000 nm in 1 nm steps without ever changing a filter. Sample absorbances can be scanned over the whole spectrum either in microplates or cuvettes.

Typical applications of the Multiskan Spectrum

• UV/Visible spectrophotometry
• Spectrum scan
• Nucleic acid analysis
• RNA concentration (A260)
• ssDNA concentration (A260)
• dsDNA concentration (A260)
• DNA purity (A260/A280)
• Enzyme kinetics
• E.g.,Vmax and Km
• Protein analysis
• Biuret protein determination
• Bradford protein determination
• BCA protein determination

Technical Specifications

Performance

• Wavelength range 200 – 1000 nm
• Read-out range 0 – 4 Abs
• Linearity 0 – 3 Abs, ±/- 2% at 450 nm
• Accuracy ± 1% or ± 0.005 Abs (0 – 2 Abs)
• ± 2.0% (2 – 3 Abs)
• Precision CV < 1% (0 – 2 Abs)
• CV < 2% (2 – 3 Abs)
• Optical bandpass 2 nm
• Wavelength accuracy ± 1 nm
• Stray light < 0.02% at 230 nm

General Specifications

• Microplate types 6-, 12-, 24-, 48-, 96- and 384-well plates
• Wavelength selection Tunable monochromator, 200 – 1000 nm in increments of 1 nm
• Light source 10 W Xenon flash lamp
• Detectors Photodiodes
• Incubator temperature range Ambient (+25°C) +4°C to 45°C
• Mains input 100 – 240 V (50/60 Hz) autoranging
• Power consumption 200 VA
• Dimensions 397 mm (W) x 217 mm (H) x 450 mm (D)
• [15.6” (W) x 8.5” (H) x 17.7” (D)]
• Weight 12.5 kg (27.5 lbs.)