Used Lab Equipment

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Includes PM From BD and Guaranteed Installable under BD Service Contract

New Flow Cell installed in September 2018

Also included are the excitation laser lines, laser powers and optical configuration.

The new BD FACSAria IIu cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria IIu cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria IIu cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cellsorting.

Includes:

• 4 lasers (375nm, 488nm, 405nm, 633nm)
• Blue 7PMTs, Red 3PMTs, Violet 3PMTs
• HP Computer w/ FACSDiva 8.0 Software
• ACDU
• 1 HP monitor
• Fluidics Cart
• Temperature Control
• Lauda Chiller
• Buffalo Whisper Aerosol Control
• Under BD service contract since installed in 2012

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Features:

• Agilent G2505B Micro Array Scanner

Specifications:

• Voltage:115 VOLTS115 VOLTS
• Frequency:50/60 HERTZ50/60 HERTZ

Description:

The Welch 8890A, GEM 1.0 is a durable vacuum pump with a superior vapor handling capability. The Welch 8890A, Direct Drive Vacuum dual stage rotary vane mechanical vacuum pump offers an excellent ultimate pressure of  1 x 10-1 Torr. These pumps are commonly used for vacuum furnace evacuation, backing turbomolecular pumps, backing diffusion pumps, rotary evaporation, refrigeration system evacuation, epoxy degassing, vacuum sterilization, backing mass spectrometers, freeze drying, vacuum centrifugal applications, vacuum distillation, space research, and other vacuum processes. This pump also is well suited for use in gas analyzers, continuous gas-flow lasers, general-purpose drying, and similar uses where a middle-range vacuum level is required.

Specifications:

• Ultimate Pressure (Torr): 1 x 10-1
• Sound Level (dBA): 58
• Motor/Pump Speed (60 Hz): 3450
• Weight: 24.5 lbs. (11.1 kg)
• Dimensions (L x W x H): 14.5 x 5.1 x 8.4” (36.8 x 13 x 21.3 cm)

Includes:

• Computer w/ GenePix Pro -  MicroArray Acquisition & Analysis Software
• Flat Panel Monitor
• All Necessary Cords, Cables
• Manual

Features:

• Speed. Unlike most commercially available microarray scanners, the GenePix 4000B Microarray Scanner acquires data at two wavelengths simultaneously. This means drastically reduced scan times and hence greater workflow efficiency. Apart from superior speed, simultaneous scanning eliminates potential errors associated with aligning two separately scanned images, and provides real-time access to data as they are acquired.
• Add Reliability to Your Research. The performance calibration standards that come with your GenePix 4000B Microarray Scanner allow you to check the performance when you receive your scanner and at any time in the future. GenePix Pro Acquisition and Analysis Software includes one-touch calibration to restore any GenePix scanner to benchmark performance. You can rest assured knowing the signals you generate today are the same as a couple of years ago and in the future.
• Automate Your Workflow. With automated photomultiplier (PMT) balancing. The fully integrated GenePix Pro Acquisition and Analysis Software, controls the GenePix 4000B Microarray Scanner and can automatically evaluate the required PMT gain values, for fast and easy optimization of signal intensity and channel balance. The GenePix 4000B Microarray Scanner then acquires data at your selected resolution between 5-100 micron, allowing the adjustment of image resolution and file size for each experiment.
• Get More Published. For over 10 years and close to 6000 literature references, the GenePix 4000B Microarray Scanner has expanded the overall sample compatibility, with user-adjustable focus offset and laser power settings. This allows proper imaging of slides with either a raised surface, as with membrane-coated glass, or a recessed surface, as in embedded arrays.

Performance Specifications

• Sample type: Standard microscope slide (1" x 3", 25 x 75 mm or 26 x 76 mm)
• Scan area: Adjustable, 22 x 71.5 mm max.
• Excitation: 532 nm and 635 nm solid-state lasers
• Laser power settings: User-selectable 100%, 33% or 10% laser power
• Emission filters: Two, optimized for Cy3 and Cy5 or spectrally similar dyes
• Detection: Dual photomultipliers (PMTs), automatic and manual gain adjustment
• Focus offset: Adjustable between -50 and +200 μm (5 μm increments)
• Optics: Non-confocal
• Scanning method: Simultaneous, 2-channel
• Scan time:5 minutes for both channels, 10 μm resolution, full scan area
• Pixel resolution: Adjustable from 5-100 μm
• Digital resolution: 16-bit
• Dynamic range: Four orders of magnitude at SNR > 3
• Image type: Single- or multi-image TIFF
• Barcode reading: Image-based software barcode reader

General Specifications

• Dimensions (in.):5 (W) x 8.0 (H) x 17.5 (D)
• Dimensions (cm): 34 (W) x 20 (H) x 44 (D)
• Weight: 45 lbs. (20 kg)
• Power supply: 110/220V universal
• Genomics: Directed at the genome sequence itself, microarrays have been used to identify novel genes, binding sites of transcription factors, changes in DNA copy number, and variations from a baseline sequence, such as in emerging strains of pathogens or complex mutations in disease-causing human genes.

Features:

Mini 48 DNA purification instrument is used to automatically purify plasmid DNA directly from bacterial culture in just over one hour.

Specifications:

• Electrical: 110V

Features:

The Muse Cell Analyzer delivers accurate assessments of cell health parameters, cell signaling activation pathways & immune status in just minutes, revolutionizing the way you analyze cells.

Specifications:

• Cell Number per Assay: User defined; cell concentrations of 10,000–500,000 cells/mL
• Sample Format: Single loader
• Sample Volume: 200 µL
• Emission Max: Yellow: 28nm bandwidth at 576nm, Red: 30nm bandwidth at 680nm, Forward scatter = laser wavelength
• Excitation Max: Green Laser (532nm)
• Scatter: Forward
• Throughput: Less than 2 minutes per sample
• Dimensions 8.69” H  x 8.12” W x 11.11” L

"Fully automated and easy-to-use, the nCounter MAX Analysis System provides everything you need to cost-effectively complete your projects in record time. More accurate and highly multiplexed than qPCR and simpler than NGS, the nCounter MAX allows for digital examination of multiple pathways in a single tube in an extraction-free workflow requiring only 15 minutes of hands on time. Accelerate your research by spending less time on sample prep and perform your own data analysis using the included nSolver Analysis Software.

Specification:

Level of multiplexing: Up to 800 targets

Recommended amount of starting material (dependent on assay and sample type)
RNA: 1 ng - 50 ng
DNA 5 ng - 300 ng
Protein: 250 ng - 2.5 µg
Sample types supported: Total RNA, cell lysates in GITC, FFPE-derived total RNA, and PAXgene-lysed whole blood
Reaction volume: up to 30 µg
Limit of detection 0.5 fM spike-in control: 15 zeptomole spike-in control

Fold change sensitivity
>1.5-fold (if > 5 copies per cell)
> 2-fold (if > 1 copy per cell)
Spike correlation: R² ≥ 0.95
Linear dynamic range: 6 x 10⁵ total counts
Prep Station throughput: 12 lanes per 2.5 hours
Digital Analyzer throughput: 12 samples per 2.7 hours
Controls: Assay dependent
Operating temperature: 18–28°C
Humidity: 30-80% (non-condensing)
Pollution degree: 2
Power source: 30-80% (non-condensing)
Power draw: 610 VA
Dimensions (Prep Station): 67 x 89 x 63 cm
Dimensions (Digital Analyzer): 66 x 66 x 48 cm
Weight (Prep Station): 120 kg
Weight (Digital Analyzer): 67 kg"

The New Brunswick INNOVA 4200 Series are benchtop gyratory incubator shakers. Designed for versatility, these shakers are multipurpose instruments. Fitted with a front-opening door, the INNOVA 4200 shakers can be used on or under the bench, or floor-stacked for space savings.

Specifications:

• Accessory Platform Size: 18 x 18 in
• Maximum Capacity: 18 x 250 mL
• Capacity and Largest Flask Size: 2 x 6 L
• Orbit Diameter:75 in
• Temperature Range: Ambient +5 to 80 °C
• Agitation: 25 – 400 rpm
• Overall Dimensions: 4 x 28.7 x 36.4
• Weight: 270 lbs

Experience industry-leading performance in carbon and sulfur analysis with the rapid CS cube: The elemental analyzer offers simultaneous, fast and automated analysis of carbon and sulfur, making the rapid CS cube a universal instrument for the analysis of coal and coke, soil samples, or waste. Designed for unattended 24/7 operation with highest possible instrument uptime, the rapid CS cube delivers results with highest precision and accuracy at outstanding low maintenance.

Specifications:

• Weighing range: The instrument’s range encompasses micro (< 1 mg) and macro (1 g) applications, depending on the sample type.
• Element concentration range: C: up to 40 mg absolute, from 0 - 100 %; S: up to 20 mg absolute, from 0 - 100 %
  Precision: < 1 % relative (homogeneous substance), depending on sample type, analysis mode, and configuration
• Analysis time: ~ 3-5 min, self-optimizing according to element content and sample weight. Depending on sample type, analysis mode, and configuration.
• Detector: SO₂and CO₂ specific infrared detector for sulfur and carbon analysis
• Maximum furnace temperature: 1,200 ° C (whereby 1,800 °C is briefly reached at the combustion point when using tin foil)
• Required gases: Oxygen or synthetic air

Experience nitrogen and protein analysis with increased laboratory efficiency. The rapid MAX N exceed nitrogen and protein analyzer operates according to the safe, simple, and eco-friendly Dumas combustion method and offers fast and cost-effective nitrogen and protein determination in just five minutes. Designed for unattended 24/7 operation, the analyzer addresses the needs of any high-throughput laboratory facing a wide range of sample types and weights. The rapid MAX N exceed stands for outstanding precision, sensitivity, and sample flexibility.

Specifications:

• Maximum sample weight: Up to 5 g or 5 ml 
• Element concentration range: up to 500 mg absolute, from 20 ppm to 100 % 
• Precision: helium: < 0.05 % absolute (250 mg glutamic acid) ; argon: < 0.15 % absolute (250 mg glutamic acid) 
• Analysis time: ~ 4-5 min, self-optimizing according to element content and sample weight 
• Detector: Thermal Conductivity Detector (TCD) 
• Sample introduction: Sample introduction via integrated 90 position autosampler, reloadable during analysis.
• Maximum furnace temperature: 1,200 °C 
• Required Gas: helium or argon and oxygen

The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

Includes:

• Computer w/ Software
• Monitor
• Manuals

Product Features:

• Automation of the standard trypan blue assay
• % Viability
• Total cell concentration
• Total viable cell concentration
• Mean cell size
• Real time cellular images
• Bioprocess monitoring
• Validated reagents
• Convenient reagent pack

 The Vi-CELL XR offers additional benefits over the S and AS Models:

• Extended size range to 2 microns, allowing additional analyses of yeast, and smaller cells.
• Increased sample throughput with greater efficiency.
• Reduced sample volume results in less reagent use.
• Auto focus capability increases instrument ease of use.
• Variable aspiration and mixing cycles optimize results.
• Enhanced image magnification provides unparalleled cellular detail.
• Circularity measurement helps isolate debris from cells.

 Specifications:

• Analysis Time: < 2.5 min
• Capacity: Single or 12 position via autoloader
• Concentration Range: 5 x 10^4 to 1 x 10^7 cells/mL
• Counting Accuracy: ± 6%
• Depth: 41 cm (16 in)
• Digitizing Resolution: 1394 x 1040
• Height: 44.5 cm (17.5 in)
• Imaging Technology: Auto-focus CCD array, Firewire camera
• Instrument Type: Video image through a quartz flow cell
• Overall Analysis Range: Size Range: 2 µm - 70 µm
• Sample Volume: 0.5 mL
• Temperature: 10°C to 40°C (50°F to 104°F)
• Viability Range: 0 to 100%
• Weight: 11.3 kg (25 lb)
• Width: 38 cm (15 in)

With its basic ease of use and abundant functions, the XRD-6000 boasts an integrated design featuring a vertical goniometer and data processing software supporting the Windows XP user interface.

The XRD-6000 offers solutions encompassing wide-ranging analysis requirements, from routine qualitative and quantitative analysis to state change analysis, including stress analysis, residual austenite quantitation, crystallite size / lattice strain, crystallinity calculation, materials analysis via overlaid X-ray diffraction patterns, enhanced material evaluation and sample heating analysis. And, of course, crystalline structural analysis is also supported, including precise lattice constant determination and crystal system determination.