Cambridge Scientific
Used Lab Equipment

Used Lab Equipment

We offer an assortment of used and refurbished Flow Cytometers and Cell Sorters. Some of our popular manufacturers include Beckman-Coulter, BD Biosciences (Becton-Dickinson) and Guava.

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BD BioScience FACSCalibur Cell Analysis

BD BioScience FACSCalibur Cell Analysis

Cambridge ID #: 18426

Includes Delivery, Installation, and recent PM

 

The flow cytometer for your routine cell analysis needs.  The BD FACSCalibur platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system. Designed with multicolor application support in mind, the BD FACSCalibur flow cytometer provides flexibility to support a wide variety of research and clinical applications.

The BD FACS Calibur system is the first benchtop flow cytometry system available that is capable of sorting cells of interest for further study.   With the BD FACS Calibur system, sorting is routine—just gate the population of interest and click ACQUIRE—the BD FACS Calibur system does the rest. Sorting occurs in a completely enclosed, aerosol-free environment for enhanced safety when processing biohazardous samples. Cells sorted by the BD FACS Calibur system are collected into test tubes, or if desired, can be deposited directly onto filters or cell culture inserts with the Cell Concentrator Module option.

 

Includes:

  • 3 Tube Sorting Capacity
  • 2 Lasers
    1. 488 Saphire
    2. 635 Red Diode
  • 4 Color System (Green, Yellow, Red, Infared)
  • FITC, PE, APC, PerCP
  • MAC Computer w/ Cell Quest Pro software
  • 2 Monitor
  • Manuals

 

Performance

  • Fluorescence Sensitivity - Estimated detection limit: 750 molecules of equivalent soluble fluorescein.
  • Fluorescence resolution Coefficient of variation in FL2-Area of <3%, full peak for propidium iodidestained chicken erythrocyte nuclei.
  • Forward and side scatter sensitivity - Separation of fixed platelets from noise.
  • Forward and side scatter resolution - Optimized scatter performance for resolving lymphocytes, monocytes, and granulocytes.

 

Excitation Optics

  • Optical platform - Fixed optical assembly
  • Lasers -Air-cooled argon-ion laser, 15 milliwatt, 488 nm; life expectancy >5,000 hours.
  • Beam geometry - Prismatic expander and achromatic spherical lens providing 22 x 66-μm elliptical beam for argon-ion laser.
  • Nominally 15 x 61-μm elliptical beam for red-diode laser.

 

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BD BioScience FACSCanto II 3 Laser  Flow Cytometer

BD BioScience FACSCanto II 3 Laser Flow Cytometer

Cambridge ID #: 18402

Recent PM Guaranteed Installable:

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

 

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

 

Includes:

  • Computer w/ FacsDiva Software
  • Fluids Cart
  • 3 laser, Blue(488) and Red (633) and Violet (405)
  • 8 PMTs in 4-2-2 configuration w/ side scatter
  • All Necessary Cables
  • Manuals

 

Optics

Lasers

  • Air-cooled
  • 488-nm solid state, 20-mW laser output
  • 633-nm HeNe, 17-mW laser output
  • 405 nm Violet laser
  • Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
  • Optical Alignment Procedure: Fixed, no operator alignment required
  • Flow Cell: 180 x 430-μm rectangular quartz flow cell
  • Collection Optics: Optical-gel coupled 1.2 NA lens
  • FSC Resolution: 0 μm
  • SSC Resolution: 5 μm
  • Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
  • FSC Detector: Photodiode with 488/10 BP
  • SSC Detector: PMT with 488/10 BP
  • Fluorescence Detectors: 8 PMTs in 4-2-2 configuration w/ side scatter
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BD BioScience Accuri C6 Flow Cytometer

BD BioScience Accuri C6 Flow Cytometer

Cambridge ID #: 18125

Features:

  • BD CSampler- 96 well plate and 24 tube rack automated sampler for BD Accuri C6 flow cytometer  
  • Blue and red laser
  • Two light scatter detectors
  • Optical filters such as FITC, PE, PerCP and APC
  • A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use
  • Optional filters and the Selectable Laser Module expand the available fluorochrome combinations
  • Compact system and portable weight (13.6 Kg)
  • Intuitive interface of the software
  • Measures: 11 x 14.75 x 16.5 inches (H x W x D) (27.9 x 37.5 x 41.9 cm)
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BD BioScience FACSCanto II 2 Laser Flow Cytometer

BD BioScience FACSCanto II 2 Laser Flow Cytometer

Cambridge ID #: 17183

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

 

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

 

Includes:

  • Computer w/ FacsDiva Software
  • Fluids Cart
  • 2 laser, Blue(488) and Red (633)
  • 3 PMTs in 4-2 configuration w/ side scatter
  • All Necessary Cables
  • Manuals

 

Optics

Lasers

  • Air-cooled
  • 488-nm solid state, 20-mW laser output
  • 633-nm HeNe, 17-mW laser output
  • Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
  • Optical Alignment Procedure: Fixed, no operator alignment required
  • Flow Cell: 180 x 430-μm rectangular quartz flow cell
  • Collection Optics: Optical-gel coupled 1.2 NA lens
  • FSC Resolution: 1.0 μm
  • SSC Resolution: 0.5 μm
  • Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
  • FSC Detector: Photodiode with 488/10 BP
  • SSC Detector: PMT with 488/10 BP
  • Fluorescence Detectors: 6 PMTs in 4-2 configuration w/ side scatter
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BD BioScience FacsAria IIu Cell Sorter

BD BioScience FacsAria IIu Cell Sorter

Cambridge Scientific ID #'s: 14996, 17981

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

 

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

 

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

 

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

 

Includes:

  • 3 lasers
  • Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
  • Computer w/ FACSDiva 8 Software
  • ACDU
  • 2 monitors
  • Fluidics Cart
  • Temperature Control
  • Lauda Chiller

 

Performance

Fluorescence Sensitivity

  • Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
  • FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
  • PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

  • Coefficient of variation (CV)
  • PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
  • Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

  • Doublet/singlet ratio:
  • CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

  • Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

  • Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

  • Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.
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BD BioScience FacsCanto II - 3 Laser Flow Cytometer

BD BioScience FacsCanto II - 3 Laser Flow Cytometer

Cambridge ID #: 12160

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

 

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

 

Includes:

  • Computer w/ FacsDiva Software
  • Fluids Cart
  • 2 laser, Blue(488) and Red (633)
  • Laser
  • 6 PMTs unit and side scatter
  • All Necessary Cables
  • Manuals

 

Optics Specifications:

  • Lasers
    • Air-cooled:
    • 488-nm solid state, 20-mW laser output
    • 633-nm HeNe, 17-mW laser output
  • Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
  • Optical Alignment Procedure: Fixed, no operator alignment required
  • Flow Cell: 180 x 430-μm rectangular quartz flow cell
  • Collection Optics: Optical-gel coupled 1.2 NA lens
  • FSC Resolution: 0 μm
  • SSC Resolution: 5 μm
  • Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
  • FSC Detector: Photodiode with 488/10 BP
  • SSC Detector: PMT with 488/10 BP
  • Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
  • Blue Laser Dyes
    • FITC, PE, PerCP or PerCP-Cy™5.5,
    • PE-Cy7 (525, 575, 678 or 695, 785 nm)
  • Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
  • Detector Bands
    • Blue Laser: 530/30; 585/42; >670; 780/60 nm
    • Red Laser: 660/20; 780/60 nm
  • Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF
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