Used Lab Equipment

The HP / Agilent 1100 HPLC series is the current model and arguably the best design in the industry because of the optimized design of the quaternary pump ensuring virtually pulse-free and stable solvent flow, with dual floating pistons in series, precisely servo-controlled. This design with variable stroke volume allows pulse-free solvent delivery and efficient mixing. An inlet-valve electronically activated and synchronized to piston stroke - eliminates vapor formation with volatile solvents and is maintenance free. With high-speed proportioning valve and pre-configured vacuum degasser, for convenient low-pressure mixing of up to four solvents and highest flexibility in solvent choices.

 Features:

• Ease-of-use - handheld control module lets you enter operating parameters fast, store methods easily and display pressure profile, diagnostics and task-oriented information.
• Durable - stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE ensure long lifetime. Seal wash option for high salt mobile phases keeps maintenance to a minimum for lower operation costs.
• Wide flow range - up to 10 ml/min for broad HPLC work, including semi-preparative separations.
• Better for GLP - built-in leak and pressure test with graphical presentation of pressure profiles, and early maintenance feedback.

Specifications:

• Hydraulic system: Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons and active inlet valve
• Settable flow range: .001– 10 ml/min, in 0.001 ml/min increments
• Flow range: 2 – 10.0 ml/min
• Recommended pH range: – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel.
• Operating voltage: 230V

The G1311A is an optimized design ensuring virtually pulse-free and stable solvent flow with dual floating pistions.  An inlet valve electronically activated and synchronized to piston stroke eliminates vapor formation with volatile solvents and is maintenance free.  Instrument comes equipped with four solvent inlets with built-in gradient control. 

Specifications:

• Configurable Flow Range: 0.001 – 10ml/min – in 0.001ml/min increments
• Flow Range: 0.2 – 10.0 ml/min
• Recommended pH Range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
• Gradient Formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve. Delay volume 800-1100µl, depending on back pressure

The G1311A is an optimized design ensuring virtually pulse-free and stable solvent flow with dual floating pistions.  An inlet valve electronically activated and synchronized to piston stroke eliminates vapor formation with volatile solvents and is maintenance free.  Instrument comes equipped with four solvent inlets with built-in gradient control. 

Specifications:

• Configurable Flow Range: 0.001 – 10ml/min – in 0.001ml/min increments
• Flow Range: 0.2 – 10.0 ml/min
• Recommended pH Range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
• Gradient Formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve. Delay volume 800-1100µl, depending on back pressure

The G1311A is an optimized design ensuring virtually pulse-free and stable solvent flow with dual floating pistions.  An inlet valve electronically activated and synchronized to piston stroke eliminates vapor formation with volatile solvents and is maintenance free.  Instrument comes equipped with four solvent inlets with built-in gradient control. 

Specifications:

• Configurable Flow Range: 0.001 – 10ml/min – in 0.001ml/min increments
• Flow Range: 0.2 – 10.0 ml/min
• Recommended pH Range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
• Gradient Formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve. Delay volume 800-1100µl, depending on back pressure

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack

Specifications:

• Injection range: From 0.1 – 100 µl in 0.1 µl increments
• Replicate injections: From 1 – 99 from one vial
• Injection cycle time: Typically 50 s, depending on draw speed and volume
• Sample capacity: 100 × 2 ml vials in tray

Specification:

• Detection type: Double-beam photometer
• Light source: Deuterium lamp
• Wavelength range: 190–600 nm
• Short term noise (ASTM): ±0.75×10^-5 AU at 254 nm
• Drift: 3 × 10 ^-4 AU/hr at 254 nm
• Linearity: 2 AU (5%) upper limit
• Wavelength accuracy:
• Band width: 5 nm typical
• Flow cells:
  • Standard: 14-μl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximum
  • High pressure: 14-μl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximum
  • Micro: 1-μl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximum
  • Semimicro: 5-μl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum
• Control and data evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, one output
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter
• Housing: All materials recyclable.

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

Optional Column Selection Valve

Specifications:

• Temperature Range: 10° below ambient to 80° C
• Temperature Stability: ± 0.15°C
• Temperature Accuracy: ± 0.8°C
• Column Capacity: Three 30cm columns
• Warm-up/Cool Down: 5 mins from ambient to 40°C

Optional Column Selection Valve

Specifications:

• Temperature Range: 10° below ambient to 80° C
• Temperature Stability: ± 0.15°C
• Temperature Accuracy: ± 0.8°C
• Column Capacity: Three 30cm columns
• Warm-up/Cool Down: 5 mins from ambient to 40°C

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

• Simplified optical design for optimized baseline stability.
• Long-life Xenon lamp for highest sensitivity.
• Variable-mode monochromators for selective multisignal detection and online spectra acquisition.
• Easy front access to flow cell for fast inspection or exchange.

Specifications

• Detection type:  Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis
• Performance:  10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H20) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
• Light source: Xenon flash lamp, normal mode (20W) and economy mode (5W)
• Pulse frequency: 296 Hz for signal mode, 74 Hz for economy mode
• Excitation monochromator: Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm
• Emission monochromator: Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm
• Reference system: Inline excitation measurement
• Time programing: Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters
• Spectral acquisition: Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step
• Step size: 1—20 nm
• Spectra storage: Limited only by disk space
• Wavelength repeatability: ±0.2 nm
• Wavelength accuracy: ±3 nm
• Flow cells: Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz
• Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, 2 outputs
• Communications: Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

Specifications:

• Maximum flow rate: 10 ml/min per channel
• Number of channels: 4
• Internal volume per channel: Typically 12 ml per channel
• Materials in contact with solvent: PTFE, PEEK
• pH range: 1 – 14
• Analog output (AUX): For pressure monitoring, range 0 – 3 V