Used Lab Equipment

Specifications:

• Detection type: 1024-element photodiode array
• Light source: Deuterium and tungsten lamps
• Wavelength range: 190 – 950 nm
• Short term noise (ASTM) Single and Multi-Wavelength: ± 1 × 10-5AU at 254 and 750 nm
• Drift: 2 × 10-3AU/hr at 254 nm
• Linear absorbance Range: 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 – 400 nm
• Slit width: 1, 2, 4 , 8, 16 nm
• Diode width: < 1 nm
• Flow cells
  • Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
  • Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
  • High pressure: 1.7 µl volume, 6 mm cellpath length and 400 bar (5880 psi) pressure maximum
  • 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
• Control and data Evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter

Can be sold as an individual component or as part of a complete stack

Optional Column Selection Valve

Specifications:

• Temperature Range: 10° below ambient to 80° C
• Temperature Stability: ± 0.15°C
• Temperature Accuracy: ± 0.8°C
• Column Capacity: Three 30cm columns
• Warm-up/Cool Down: 5 mins from ambient to 40°C

Specifications:

• Maximum flow rate: 10 ml/min per channel
• Number of channels: 4
• Internal volume per channel: Typically 12 ml per channel
• Materials in contact with solvent: PTFE, PEEK
• pH range: 1 – 14
• Analog output (AUX): For pressure monitoring, range 0 – 3 V

Specifications:

• Autosampler (Chiller Compatible)
• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications: Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range: 1– 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)
• Replicate injections: 1– 99 from one vial
• Precision:
  • Typically < 0.5 % RSD of peak areas from 5 – 100 µl
  • Typically < 1 % RSD of peak areas from 1 – 5 µl
• Minimum sample volume: 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
• Carryover: Typically < 0.1 %, < 0.05 % with external needle cleaning
• Sample viscosity range: 2 – 50 cp
• Replicate injections per vial: 1 – 99
• Sample capacity:
  • 100 × 2-ml vials in 1 tray
  • 40 × 2-ml vials in ½ tray
  • 15 × 6-ml vials in ½ tray (Agilent vials only)
• Injection cycle time: Typically 50 s depending on draw speed and injection volume

Can be sold as an individual component or as part of a complete stack

The Agilent 1200 ALS Thermostat, G1330A controls temperature for either HPLCs or fraction collectors. The ALS thermostat contains Peltier-controlled heat exchangers. A fan draws air from the area above the sample vial tray of the autosampler and is then blown through the fins of the cooling/heating module. There it is cooled or heated according to the temperature setting.

Specifications:

• Weight: 20.7 kg (45.6 lbs)
• Dimensions: 140 × 345 × 435 mm (5.5 × 13.5 × 17 inches)
• Line Voltage: 100 – 120 or 220 – 240 VAC, ± 10 % Automatic selection
• Line Frequency: 50 or 60 Hz, ± 5 %
• Power consumption Autosampler: 300 VA 
• ALS Thermostat: 260 VA

Features:

• Advanced temperature controlled detector optics ready to use within two hours of installation
• Automatic zero and automatic purge combined with a recycle valve for automatic solvent recycling allow uninterrupted operation
• Automatic light intensity control circuit to ensure the optimum performance of the optics
• Integrated diagnostics for efficient troubleshooting
• Built-in refractive index calibration
• Front access to valves and capillaries for easy maintenance
•  

Specifications:

• Detection type: Refractive Index
• Refractive index range: 1.00 – 1.75 RIU, calibrated
• Measurement range: +/- 600 x 10-6 RIU
• Optics temperature control: 5 ° C above ambient to 55 ° C
• Sample cell volume: 8uL
• Maximum pressure: 5 bar (0.5Mpa)
• Maximum flow rate: 5mL/minute
• Valves: Automatic purge and automatic solvent recycle
• Volumes: Inlet port to sample cell 62uL, inlet port to outlet port 590uL

Features:

• G1364C Fraction Collector, analytical scale, designed for flow rates below 10 ml / min. (can be modified for higher flow rates) and for the use with vials, test tubes of up to 75 mm height, well-plates and a 40-funnel tray connecting to external locations of any size 

• For analytical scale applications

Specifications:

• Trigger Modes: Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
• Operating Modes: Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
• Test tube /plate sizes: Minimum 48 mm to 100 mm maximum
• Maximum tube volume: ca. 45 ml
• Maximum flow rate: 100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
• Delay volumes [μl]:
• Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
• Diverter valve: ~15
• Diverter valve to needle: ~110
• Needle: -~5
• Delay calibration Sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
• Diverter Valve: 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
• Cooling: Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions
• Maximum capacity: 3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
• GLP features Early maintenance feedback (EMF), electronic records of maintenance and errors
• Interfaces: Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
• Safety features: Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Can be sold as an individual component or as part of a complete stack

The Agilent 1200 Series autosampler is designed for use with other modules of the Agilent 1200 Series LC system or with other LC systems if adequate remote control inputs and outputs are available. The autosampler is controlled from the Agilent 1200 Series control module or from the Agilent ChemStation

for LC systems.

Specifications:

• Weight: 15.5 kg (34.2 lbs)
• Dimensions (height × width × depth): 200 × 345 × 440 mm (8 × 13.5 × 17 inches)
• Line voltage: 100 – 240 VAC, ±10 % Wide-ranging capability
• Line frequency: 50 or 60 Hz, ±5 %
• Power consumption (apparent power): 300 VA Maximum
• Power consumption (active power): 200 W Maximum
• Ambient operating temperature: 4 to 55 8C (41 to 131 8F)
• Ambient non-operating temperature: -40 to 70 8C (-4 to 158 8F)
• Humidity: < 95 %, at 25 to 40 8C (77 to 104 8F) Non-condensing
• Operating Altitude: Up to 2000 m (6500 ft)
• Non-operating altitude: Up to 4600 m (14950 ft) For storing the autosampler

The Agilent 1200 Series autosampler is designed for use with other modules of the Agilent 1200 Series LC system or with other LC systems if adequate remote control inputs and outputs are available. The autosampler is controlled from the Agilent 1200 Series control module or from the Agilent ChemStation

for LC systems.

Specifications:

• Weight: 15.5 kg (34.2 lbs)
• Dimensions (height × width × depth): 200 × 345 × 440 mm (8 × 13.5 × 17 inches)
• Line voltage: 100 – 240 VAC, ±10 % Wide-ranging capability
• Line frequency: 50 or 60 Hz, ±5 %
• Power consumption (apparent power): 300 VA Maximum
• Power consumption (active power): 200 W Maximum
• Ambient operating temperature: 4 to 55 8C (41 to 131 8F)
• Ambient non-operating temperature: -40 to 70 8C (-4 to 158 8F)
• Humidity: < 95 %, at 25 to 40 8C (77 to 104 8F) Non-condensing
• Operating Altitude: Up to 2000 m (6500 ft)
• Non-operating altitude: Up to 4600 m (14950 ft) For storing the autosampler

Comprises a 4-channel vacuum container, including 4 tubular plastic membranes, and a vacuum pump.

Specifications:

• Maximum flow rate:
  • 10 ml/min per channel
  • 5-10 ml/min per at reduced degassing performance
• Number of channels: 4
• Internal volume per channel: Typically 12 ml per channel
• Materials in contact with solvent: PTFE, PEEK
• pH range: 1 – 14
• Analog output (AUX): For pressure monitoring, range 0 – 3 V

Can be sold as an individual component or as part of a complete stack

This Agilent 1200 Model G2226A ( G 2226A, G 2226 A, G2226 A ) Series Nano (NanoFlow) Pump is in excellent condition!  The Agilent 1200 Series nanoflow pump uses a unique solvent delivery technology optimized for nanoliter-per-minute flows and features EFC (Electronic Flow Control) with active feedback and real time flow adjustment. EFC is the gold standard for robust and reliable nanoflow performance and delivers the precise flow and gradient performance necessary for retention time reproducibility and stable ion generation essential for high sensitivity nanospray MS performance.

Features:

• Electronic Flow Control (EFC) provides real-time adjustment of the flow rate throughout the analysis for reliable and reproducible results.
• Micro vacuum degassing with very low internal volume per channel (1 mL) for fast changeover of mobile phase and quick purge.
• Control from handheld controller for convenient local status monitoring.
• 2nd generation flow sensors.
• Application focus: High resolution separations and high sensitivity MS detection. Proteomics is a typical application area for the nanoflow pump.

Specifications:

• Pressure: Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5800psi)
• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications: Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range:
  • 900 µl in 0.1 µl increments (recommended 1 µl increments)
  • Up to 1800 µl with multiple draw (hardware modification required)
  • Up to 5000 µl with multiple draw (hardware modification required)
• Replicate injections: – 99 from one vial
• Precision:
  • Typically < 0.5% RSD of peak areas from 5 – 2000 µl,
  • Typically < 1% RSD of peak areas from 2000 – 5000 µl
  • Typically < 3% RSD of peak areas from 1 – 5 µl
• Minimum sample volume:
  • µl from 5 µl sample in 100 µl microvial
  • 1 µl from 10 µl sample in 300 µl microvial
• Sample viscosity range: 0.2 – 50 cp
• Sample capacity:
  • 100 × 2-ml vials in 1 tray
  • 15 × 6-ml vials in ½ tray (Agilent vials only)
• Injection cycle time: Typically 50 s, depending on draw speed and injection volume

Optional Column Selection Valve

Specifications:

• Temperature Range: 10° below ambient to 80° C
• Temperature Stability: ± 0.15°C
• Temperature Accuracy: ± 0.8°C
• Column Capacity: Three 30cm columns
• Warm-up/Cool Down: 5 mins from ambient to 40°C

Features:

This Agilent 1220 Infinity LC - G4288B has a Gradient Pump, Manual injector, Variable wavelength detector configuration.


Specifications:

Weight: 43 kg (94 lbs)
Dimensions (height × width× depth): 640×370×485 mm (25.2×14.6×19.1 inches)
Line frequency: 50 or 60 Hz, ± 5 %
Power consumption: 240 VA / 210 W / 717 BTU
Ambient operating temperature: 4–55 °C (39–131 °F)
Ambient non-operating temperature: -40 – 70 °C (-40 – 158 °F)
Humidity: < 95 % r.h. at 40 °C (104 °F)

Features:

This versatile and highly reliable pump offers flow rates up to 10 mL/min and pressures up to 600 bar. It is suited for the use of smaller particle size columns giving higher resolution and faster separations. It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual floating, precise servo-controlled pistons adjust the stroke volume according to your chosen flow rate. Offering access to up to four solvents, the 1260 Infinity II Quaternary Pump provides the greatest flexibility in automated solvent blending. It is recommended for a wide range of research and routine applications (especially in the food, environmental and pharmaceutical sectors) as well as for method development.

Specifications:

• Higher resolution, faster separations - the maximum pressure of 600 bar allows you to use smaller particle size columns, longer columns and/or higher viscous solvents as an alternative to acetonitrile.
• Analytical and semi-preparative purifications - the wide flow range (up to 10 mL/min) perfectly supports standard and semi-preparative applications. The perfect choice for 4.6 and 3.0 mm id columns.
• Quicker experiments - the pump offers convenient access to up to four solvents for isocratic or gradient analysis, which can really speed up method development, preparation of mobile phases and system flushing. 
• Easy, fast and secure transfer of methods - from conventional to sub-2-micron columns.
• Integrated degasser
• Longevity and low operating costs - robust materials (stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE) withstand the most demanding applications and keep your pump functioning for years. Our optional seal wash for high-salt mobile phases keeps maintenance to a minimum and reduces operating costs.