Used Lab Equipment

Asking Price: $11,150 w/ 1 year manufacturer warranty and 25,000 hour lamp guarantee

List Price: $12,360 (We are discounting 10% off List)

X-Cite XLED1 configurations includes: X-Cite XLED1 illumination system, four modules, user manual (CD) with GUI and USB driver, quick start guide, liquid light guide, microscope adaptor, power cord, four excitation filter holders, USB cable

The X-Cite XLED1 is a high-power LED light source designed to optimize fluorophore excitation with unmatched LED switching speeds. Its unique plug-and-play modularity allows the system to evolve alongside changing research applications, with easily interchangeable LED modules. With unprecedented wavelength switching speeds to capture fast cell dynamics and advanced triggering options including X-Cite Live Cell Mode to extend live-cell imaging experiments, the X-Cite XLED1 represents the industry's next generation of fluorescence LED illumination.

Features:

• Maximum power and individual LED control
• Unmatched field uniformity at the specimen
• Adaptable plug-and-play modularity
• Rapid wavelength switching to capture fast cell dynamics
• Flexible triggering for sequential or simultaneous imaging
• X-Cite Live Cell Mode to limit photobleaching and cellular damage

Benefits:

• Use up to four high power LED modules with fine excitation control while balancing illumination intensity between channels and protecting specimens against photodamage.
• X-Cite microscope adaptors lead the industry in field uniformity without the need for alignment, while saving time on maintenance and ensuring peace-of-mind in experimental results.
• Each LED module and associated optical components can be quickly swapped in the field for another wavelength depending on the needs of your application.
• Each LED module is designed to integrate and easily interchange individual excitation filters, allowing accelerated wavelength switching beyond the scope of motorized filter wheels.
• Triggering sequences can be combined with a high degree of control over individual LED intensity to excite and image multiple fluorophores when examining very fast moving specimens or for live-cell ratio imaging.
• Researchers can extend the time frame of their live-cell imaging experiments by reducing the degree of free radical formation caused by the continuous illumination of fluorescent proteins.

Specifications

• Wavelengths: Wavelengths and configurator available at http://www.excelitas.com/X-Cite-LED-Module-Configurator.aspx
• Dimensions: 8.5″ (W) x 8″ (H) x 15″ (D) (22cm x 20cm x 39cm)
• Weight: ~7.3kg, ~16lbs (full system)
• Supply: Universal input of 100-240Volts AC, 50-60Hz. Fused at 6.3A
• Connectivity:
  • USB type-B female
  • Trigger Input BNC (TTL-compatible)*
  • Sync Output BNC (TTL-compatible)*
• Triggering:     
  • Internal/External User-defined per channel
  • Global trigger input
  • User-defined free-running or single shot pulse modes
  • Internal triggering generated from internal pulse generator 10µs (min), up to 18 hours (max)
• Sync Out: Mapped echo of LED cycles on separate output pins
• Sync Out Phase Control: Individual control of sync phase advance/retard (half of max cycle duration)
• Channel Switching Speeds: USB < 1ms; Internal/External TTL Triggering down to10μs
• Minimum Duty Cycle: 10µs

X-Cite XLED1 Touch Screen Controller: $1,495.00

X-Cite XLED1 Touch Screen Controller (Optional)

• Screen:  Medical/Industrial grade 7″ touch screen; 16:9 aspect ratio and LED backlight with adjustable intensity
• Resolution:  800 x 480 pixels
• Display Viewing Area:  6″ (W) x 3.5″ (H) (15cm x 9cm)
• External Dimensions:  8″ (W) x 2.5″ (H) x 5.5″ (D) (20cm x 7cm x 14cm)
• Program:  Lumen Dynamics proprietary graphical user interface
• Connectivity: ~2.5m connectorized power and data combo cable
• Power: < 10 watts
• Certifications: CE Marked, Certified to IEC, Canadian and US standards, RoHS compliant

Specifications:

• Autosampler (Chiller Compatible)
• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications: Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range: 1– 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)
• Replicate injections: 1– 99 from one vial
• Precision:
  • Typically < 0.5 % RSD of peak areas from 5 – 100 µl
  • Typically < 1 % RSD of peak areas from 1 – 5 µl
• Minimum sample volume: 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
• Carryover: Typically < 0.1 %, < 0.05 % with external needle cleaning
• Sample viscosity range: 2 – 50 cp
• Replicate injections per vial: 1 – 99
• Sample capacity:
  • 100 × 2-ml vials in 1 tray
  • 40 × 2-ml vials in ½ tray
  • 15 × 6-ml vials in ½ tray (Agilent vials only)
• Injection cycle time: Typically 50 s depending on draw speed and injection volume

Can be sold as an individual component or as part of a complete stack

Specifications:

• Autosampler Thermostat
• Operating temp range: 4 to 40°C (39 to 104°F)
• Operating humidity range: < 95%, non-condensing
• Maximum Power Consumption [VA]: 260 VA
• Maximum Power Consumption [W]: 210 W
• BTU: 717

Can be sold as an individual component or as part of a complete stack

Specifications:

• Autosampler Thermostat
• Operating temp range: 4 to 40°C (39 to 104°F)
• Operating humidity range: < 95%, non-condensing
• Maximum Power Consumption [VA]: 260 VA
• Maximum Power Consumption [W]: 210 W
• BTU: 717

Can be sold as an individual component or as part of a complete stack

Specifications:

• Autosampler Thermostat
• Operating temp range: 4 to 40°C (39 to 104°F)
• Operating humidity range: < 95%, non-condensing
• Maximum Power Consumption [VA]: 260 VA
• Maximum Power Consumption [W]: 210 W
• BTU: 717

Can be sold as an individual component or as part of a complete stack

Features:

• Extended flow rate range
• Without changing pump heads, provides flow rates up to 100 mL/min at 400 bar
• For preparative and analytical applications with low flow rates (0.5 mL/min, upward), provides precise high-pressure binary gradient formation with low delay volume (700 uL)
• Automated seal wash using a peristaltic pump extends seal lifetime
• Pumps stop in response to column blockage or leaks
• Automated electromagnetic purge valve simplifies system setup
• Software provides solvent level control that keeps the pump from running dry

Can be sold as an individual component or as part of a complete stack

The Agilent 1200 Series autosampler is designed for use with other modules of the Agilent 1200 Series LC system or with other LC systems if adequate remote control inputs and outputs are available. The autosampler is controlled from the Agilent 1200 Series control module or from the Agilent ChemStation

for LC systems.

Specifications:

• Weight: 15.5 kg (34.2 lbs)
• Dimensions (height × width × depth): 200 × 345 × 440 mm (8 × 13.5 × 17 inches)
• Line voltage: 100 – 240 VAC, ±10 % Wide-ranging capability
• Line frequency: 50 or 60 Hz, ±5 %
• Power consumption (apparent power): 300 VA Maximum
• Power consumption (active power): 200 W Maximum
• Ambient operating temperature: 4 to 55 8C (41 to 131 8F)
• Ambient non-operating temperature: -40 to 70 8C (-4 to 158 8F)
• Humidity: < 95 %, at 25 to 40 8C (77 to 104 8F) Non-condensing
• Operating Altitude: Up to 2000 m (6500 ft)
• Non-operating altitude: Up to 4600 m (14950 ft) For storing the autosampler

Features:

• G1364A Thermostatted Fraction Collector, preparative scale, can be created by additionally ordering and installing a G1330A Fraction Collector Thermostat

• For preparative scale applications

Specifications:

• Trigger Modes: Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
• Operating Modes: Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
• Test tube /plate sizes: Minimum 48 mm to 100 mm maximum
• Maximum tube volume: ca. 45 ml
• Maximum flow rate: 100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
• Delay volumes [μl]:
• Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
• Diverter valve: ~15
• Diverter valve to needle: ~110
• Needle: -~5
• Delay calibration Sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
• Diverter Valve: 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
• Cooling: Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions
• Maximum capacity: 3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
• GLP features Early maintenance feedback (EMF), electronic records of maintenance and errors
• Interfaces: Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
• Safety features: Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Can be sold as an individual component or as part of a complete stack

Features:

• G1364C Fraction Collector, analytical scale, designed for flow rates below 10 ml / min. (can be modified for higher flow rates) and for the use with vials, test tubes of up to 75 mm height, well-plates and a 40-funnel tray connecting to external locations of any size 

• For analytical scale applications

Specifications:

• Trigger Modes: Time slices, Peak (threshold, up- /downslope), Timetable (combination of time intervals and peak) and Manual trigger (supported only with G1323BControl Module)
• Operating Modes: Discrete fractions: default mode for all vessels. The flow is diverted to waste, while moving from one vessel position to the next vessel position Continuous flow: optional, available only when using well plates. It is possible to move from one well plate position to the next one without diverting the flow into the well plate to waste
• Test tube /plate sizes: Minimum 48 mm to 100 mm maximum
• Maximum tube volume: ca. 45 ml
• Maximum flow rate: 100 ml / min (depending on viscosity and generated back pressure, max. 6 bar at the diverter valve)
• Delay volumes [μl]:
• Fraction collector inlet to diverter valve: -500 (typical, depends on length of the tubing)
• Diverter valve: ~15
• Diverter valve to needle: ~110
• Needle: -~5
• Delay calibration Sensor: Single wavelength absorbance detector working at 654 nm, consisting of a LED and a photo diode
• Diverter Valve: 3/2 Diverter valve with low internal volume (15 μl), switching time < 100 ms, maximum operating pressure 6 bar
• Cooling: Optional (with additional G1330B), performance depending on ambient conditions and the volume of collected fractions
• Maximum capacity: 3 fraction collectors in parallel plus one recovery fraction collector connected via 12-Position, 13-Port Selector valve
• GLP features Early maintenance feedback (EMF), electronic records of maintenance and errors
• Interfaces: Controller-area network (CAN). optional; LAN or external contacts interface RS232C, APG-remote (for remote start / stop signals to / from other modules) Interface to G1330A Thermostat CAN-DC-out for operation of Agilent approved external devices like valves
• Safety features: Leak detection and safe leak handling, error detection and display, exhaust fan for fume extraction of hazardous vapors

Can be sold as an individual component or as part of a complete stack

Specifications:

• Detection type: 1024-element photodiode array
• Light source: Deuterium and tungsten lamps
• Wavelength range: 190 – 950 nm
• Short term noise (ASTM) Single and Multi-Wavelength: ± 1 × 10-5AU at 254 and 750 nm
• Drift: 2 × 10-3AU/hr at 254 nm
• Linear absorbance Range: 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 – 400 nm
• Slit width: 1, 2, 4 , 8, 16 nm
• Diode width: < 1 nm
• Flow cells
  • Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
  • Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
  • High pressure: 1.7 µl volume, 6 mm cellpath length and 400 bar (5880 psi) pressure maximum
  • 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
• Control and data Evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter

Can be sold as an individual component or as part of a complete stack

Specifications:

• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications: Communications Controller-area network (CAN). RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range:
  
  • – 8 µl in 0.01 µl increments with the small loop capillary
  • – 40 µl in 0.01 µl increments with the extended loop capillary
• Precision:
  • Typically < 0.5 % RSD of peak areas from 5 – 40 µl,
  • Typically < 1 % RSD from 1 – 5 µl
  • Typically < 3 % RSD from 0.2 – 1 µl
• Sample viscosity range: 2 – 5 cp
• Sample capacity:
  • 2× well-plates (MTP) + 10 × 2 ml vials
  • 100 x 2 ml in one tray
  • 40 x 2 ml in half tray
• Injection cycle time:
  • Typically, < 30 s using following standard conditions:
  • Default draw speed: 4 µl/min
  • Default eject speed: 10 µl/min
  • Injection volume: 0.1 µl
• Carry-over:
  • Typically < 0.05 % using the following conditions:
  • Column: 150 x 0.5 mm Hypersil ODS, 3 µm
  • Mobile phase: Water/Acetonitrile = 85/15
  • Column Flow rate: 13 µl/min
  • Injection volume: 1 µl caffeine (=25ng caffeine), 1 µl water to test carryover
  • Outside wash of needle before injection: 20 sec with water using flush port

Specifications:

• Detection type: 1024-element photodiode array
• Light source: Deuterium and tungsten lamps
• Wavelength range: 190 – 950 nm
• Short term noise (ASTM) Single and Multi-Wavelength: ± 1 × 10-5AU at 254 and 750 nm
• Drift: 2 × 10-3AU/hr at 254 nm
• Linear absorbance Range: 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 – 400 nm
• Slit width: 1, 2, 4 , 8, 16 nm
• Diode width: < 1 nm
• Flow cells
  • Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
  • Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
  • High pressure: 1.7 µl volume, 6 mm cellpath length and 400 bar (5880 psi) pressure maximum
  • 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
• Control and data Evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter

Can be sold as an individual component or as part of a complete stack

Comprises a 4-channel vacuum container, including 4 tubular plastic membranes, and a vacuum pump.

Specifications:

• Maximum flow rate:
  • 10 ml/min per channel
  • 5-10 ml/min per at reduced degassing performance
• Number of channels: 4
• Internal volume per channel: Typically 12 ml per channel
• Materials in contact with solvent: PTFE, PEEK
• pH range: 1 – 14
• Analog output (AUX): For pressure monitoring, range 0 – 3 V

Can be sold as an individual component or as part of a complete stack

Specifications:

• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications Controller: area network (CAN). RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features:  Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range:  
  • 1 – 100 μl in 0.1 μl increments
  • Up to 1500 μl with multiple draw (hardware modification required)
• Precision:
  • Typically < 0.5 % RSD of peak areas from 5 – 100 μl,
  • Typically < 1 % RSD from 1 – 5 μl
• Sample viscosity range:  0.2 – 5 cp
• Sample capacity:
  • 2 × well-plates (MTP) + 10 × 2 ml vials
  • 100x2ml in one tray
  • 40 x 2 ml in half tray
• Injection cycle time:
  • Typically < 30 s using following standard conditions:
  • Default draw speed: 200 μl/min
  • Default eject speed: 200 μl/min
  • Injection volume: 5 μl
• Carry-over Typically:
  • < 0.01 % using the following conditions:
  • Column: 125 x 4 mm Hypersil ODS, 5 μm
  • Mobile phase: Water/Acetonitrile = 80/20
  • Flow rate: 1 ml/min
  • Injection volume: 1 μl caffeine (1 mg/ml), 5 μl water to test carryover
  • Outside wash of needle before injection: 20 sec with water using flush port

Specifications:

• Sample capacity: 100 x 2 ml vials in 1 tray. Microvials (100 or 300 µl) with sleeves (reduced cooling performance with microvials)
• Settable injection volume
  • to 8 µl with small loop capillary
  • to 40 µl with extended loop capillary
• Precision
  • Typically < 0.5 % RSD from 5 to 40 µl
  • Typically < 1 % RSD from 1 to 5 µl
  • Typically < 3 % RSD from 0.2 to 1 µl
• Minimum sample volume: µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvials
• Carryover
  • Typically < 0.1 % without automated needle wash
  • Typically < 0.05 % with external needle cleaning and 1 µl injection volume
• Sample viscosity range: 2 - 5 cp
• Recommended pH-range: - 8.5 solvent with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries
• Material in contact with solvent: Stainless steel, sapphire, PTFE, PEEK, fused silica
• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications: Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features: Leak detection and safe leak handling, low voltages in aintenance areas, error detection and display
• Housing: All material recyclable

Can be sold as an individual component or as part of a complete stack