Used Lab Equipment

Specifications:

• Autosampler (Chiller Compatible)
• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications: Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range: 1– 100 µl in 0.1 µl increments Up to 1500 µl with multiple draw (hardware modification required)
• Replicate injections: 1– 99 from one vial
• Precision:
  • Typically < 0.5 % RSD of peak areas from 5 – 100 µl
  • Typically < 1 % RSD of peak areas from 1 – 5 µl
• Minimum sample volume: 1 µl from 5 µl sample in 100 µl microvial, or 1 µl from 10 µl sample in 300 µl microvial
• Carryover: Typically < 0.1 %, < 0.05 % with external needle cleaning
• Sample viscosity range: 2 – 50 cp
• Replicate injections per vial: 1 – 99
• Sample capacity:
  • 100 × 2-ml vials in 1 tray
  • 40 × 2-ml vials in ½ tray
  • 15 × 6-ml vials in ½ tray (Agilent vials only)
• Injection cycle time: Typically 50 s depending on draw speed and injection volume

Can be sold as an individual component or as part of a complete stack

Specifications:

• Sample capacity: Dependent on the carousel configuration: Vials/ Well plates
• Injection methods: Inline split-loop
• Injection volume settable range: 0.01 – 100 µL (1 – 100 µL)
• Injection volume accuracy: Typically ±0.5% at 50 and 90 µL
• Minimum sample required: 1 µL out of 5 µL (250 µL conical vial)
• Injection cycle time: <15 seconds for 5 µL

Specifications:

Noise: ±1 × 10–5 AU (254 nm, 2.0 s response time)
Drift: 2× 10–4 AU/h
Response Time: 0.1/0.2/0.5/1/2/5/10 s
Wavelengths: Up to four simultaneously, user-selectable
Wavelength Range and Accuracy: 190–740 nm, ± 2nm
Bandwidth: <8 nm
Flow Cell Volume: Nano LC, 3 nL volume; Capillary LC, 45 nL volume; Micro LC, 180 nL volume
Maximum Cell Pressure: 400 bar (6000 psi)
Lamp: Deuterium
Data Collection Rate: 1–10 Hz
Analog Output: 2×1 V
Dimensions (h × w × d): 16 × 42 ×51 cm (6.3 ×16.3 × 20 in.)
Weight: 10.5 kg (23 lb)

The Waters ACQUITY UPLC H-Class System delivers the flexibility of quaternary solvent blending with the advanced performance of UPLC® separations. The system's holistic design is targeted for routine analysis and method development use and is perfectly suited for running both HPLC and UPLC applications while still realizing the improved resolution and sensitivity of UPLC separations.

Includes:

• TUV Detector (PDA Detector $1000 additional)

• Sample Manager - FTN

• Column Manager to control up to 4 Columns
• Quaternary Solvent Manager
  • 15,000 psi up to 1.0 mL/min,
  • 9000 psi up to 2.0 mL/min
• Computer Loaded with Empower

Specifications:

• Dwell volume (total system): <400 ~L (includes standard 100 ~L mixer)
• Integrated leak management: Leak sensors, as standard, and safe leak handling
• Quantum synchronization: Injection synchronization between pump and sample manager enhances retention time reproducibility
• Set table flow rate range:
  • 01 0 to 2.000 mLlmin, in 0.001 mL increments (firmware version 1.5x and earlier)
  • 010 to 2.200 mL/min, in 0.001 mL increments (firmware version 1.60)
  • 001 to 2.200 mLlmin in 0.001 mL increments (firmware version 1.65 and later)
• Maximum operating pressure:
  • 15,000 psi up to 1.0 mL/min, 9000 psi up to 2.0 mL/min (firmware version l.5x and earlier)
  • 15,000 psi up to 1.0 mL/min, 7800 psi up to 2.2 mL/min (firmware version 1.6x and later)
• pH range: 2 to 12
• Unattended operation: Leak sensors and safe leak handling, full 96-hour diagnostic data display through console software
• Cycle time: <30s Inject to Inject

Innovative QJet ion guide technology dramatically improves sensitivity by capturing and focusing more ions generated by the efficient TURBO V ion source. And the TURBO V source virtually eliminates cross-contamination, even with high-concentration samples.  New innovations in sensitivity like QJet ion guide technology make it possible to reach the lowest limits of detection. Combining QJet ion guide technology with the proven TURBO V ion source and a powerful new generation of Analyst software results in a robust, high throughput platform ideal for all phases of pharmaceutical development.

Includes:

• AB Sciex API5000 LC/MS/MS
• Enhanced high performance triple quadrupole mass spectrometer system
• Line transformers
• Turbo V Ion Source (with APCI and ESI probes)
• Roughing Pump

• Windows 7 Computer loaded w/ Analyst Software 1.6

• Cart
• Agilent 1100 HPLC and CTC Autosampler
  • G1322 Degasser
  • G1312A Binary Pump
  • G1316A Column Compartment
  • G1323A Game Boy
  • CTC Autosampler

• Monitor – Mouse – Keyboard
• Cables and Manuals

Specifications:

• Detector type: Pulse-counting channel electron multiplier (CEM) detector
• Dynamic Range: 1 cps to 4 x 10(6) cps (pulse counting)
• Ionization Sources: ESI, APCI
• Mass Accuracy / Stability: 0.01% over the entire mass range
• Mass Range: 5 – 1250 m/z
• Scan Types:  Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM

Specifications:

• Column capacity:
  • CM-A: Two columns, as standard (maximum length of 150 mm with filter or guard column)
  • or four columns (maximum length of 50 mm) can be supported with optional tubing kit,
  • up to 4.6 mm internal diameter (1.0.)
  • CM-Aux: Two columns (maximum length of 150 mm, with filter or guard column) - up to
  • two CM-Aux units can be configured with one CM-A for support of up to six columns
• Switching valves: Two nine-port, eight-position valves (CM-A only); provides programmable, automatic, random access switching, waste and bypass positions for rapid solvent changeover
• Temperature Range:
  • 0 to 90.0 ·C, set table in 0.1 ·C increments
  • Two independent heatl cool zones per module, up to six zones in stacked configuration
• Temperature Accuracy: ±0.5 ·C
• Temperature Stability: ±0.3 ·C
• Solvent conditioning: Active pre-heating as standard
• Column tracking: eCord Technology column information management tracks and archives column usage history
• 20 support: Optional

Specifications:

• Number of sample plates: Total of two plates, expandable to up to 22 plates with optional Sample Organizer (see below):
  • 96 and 384 microtiter plates
  • 48 position 2.00-mL vial plates
  • 48 position 0.65-mL micro-centrifuge tube plates
  • 24 position 1.50-mL micro-centrifuge tube plates
• Maximum sample capacity: 768 in two 384-well plates; expandable to up to 8,448 samples with optional Sample Organizer
• Number of sample injections: 1 to 99 injections per sample
• Injection volume range: 0.1 to 50.0 iJL, in 0.1 iJL increments, partial or full loop mode; 10ul loop is standard; 1, 2, 5, 20, and 50-uL loops also available
• Sample delivery precision(full loop injection mode): < 0.3% RSO, full loop, standard lO-iJL loop (default wash/purge conditions), per Waters AQT/SystemsQT protocol
• Sample delivery precision(PLNO injection mode): < 1% RSO within 20% to 75% of loop volume for 1, 2, 5, 10, 20, and 50-iJL loops, UV detection
• Injector linearity: > 0.999 coefficient of deviation (from 20% to 75%, partial loop overfill mode (PLNO injection mode), per Waters' AQT/SystemsQT protocol
• Sample temperature control:0 to 40.0 ·C, settable in 0.1 ·C increments (assumes an ambient temperature of 25.0 ·C). At an ambient temperature of 21.0 ·C or lower the sample manager will maintain the temperature of the sample compartment down to 4.0 ·C with a tolerance of -2.0/+6.0 ·C, when configured with the maximum number of vials and/or plates
• Injection cycle time:
  • < 15 sec between multipe injections with "load ahead" enabled
  • 30 sec with single weak wash, lO-iJL loop, pressure assist mode
• Sample probe: XYZZ based needle-in-needle design
• Minimum sample required: 5 uL residual, using maximum recovery 2-mL vials (zero offset)
• Wash solvents: Two degassed: strong solvent and weak wash solvent, programmable to suit application
• Sample carryover: < 0.005% or < 2.000 nL, whichever is greater
• Advanced operations: Loop off-line mode, load ahead
• Unattended operation: Leak sensors, full diagnostic data control captured through console software
• Primary wetted material: Titanium alloy, 316 stainless steel, fluoropolymer, fluoroelastomer, PPS alloy, PEEK alloy, PPS, PEEK, OLC coating, gold

The Waters SQ Detector 2 is an advanced bench top single quadrupole mass detector designed for high performance LC/MS applications. With a wide range of ionization options including Atmospheric Pressure Photo Ionization (APPI) and the Atmospheric Solids Analysis Probe (ASAP), the SQ Detector is ideally suited to a wide range of qualitative and quantitative applications.  The system includes IntelliStart Technology, for automated system optimization and status monitoring, ensuring that the highest quality of data is routinely available to all levels of operators

Includes:

• Waters SQ Detector 2 UPLC/MS Mass Spectrometer
• Enhanced high performance single quadrupole mass spectrometer system
• Roughing Pump
• Windows 7 Computer loaded w/ Mass Lynx 4.1
• Optional Acquity UPLC
• Monitor – Mouse – Keyboard
• Cables and Manuals

Performance Specifications:

• Acquisition modes
  • Full scan MS
  • Selected Ion Recording (SIR)
• Radar: An information rich acquisition approach that allows you to collect highly specific quantitative date for targer compounds while providing the ability to visualize all other components
• Mass range: 2 to 3072 m/z
• Scan speed: Up to 15,000 Da/s
• Examples of achievable acquisition rates:
  • 15 scans per second (m/z 100 to 1000)
  • 30 scans per second (m/z 50 to 500)
• Mass stability: Mass drift is < 0.1 Da over a 24 hour period
• Linearity of response: The linearity of response relative to sample concentration for a specified compound is four orders of magnitude from the limit of detection
• Polarity switching time: 20 ms to switch between positive and negative ion modes
• EPCi mode switching time: 20ms to sqitch between ESI and APCI
• SIR acquisition rate: Minimum dwell time of 3 ms per SIR channel; Minimim inter-channel delay of 3ms
• Number of SIR channels: Up to 16,384 SIR channels (512 functions, 32 channels per function) can be monitored in a single acquisition; up to 1024 SIR channels when operating in GLP/secure mode (32 functions, 32 channels per function).
• Mass resolution: Automatically adjusted (Intellistart) to desired resolution.

Specifications:

• Optical design: Dual-beam photometer
• Light source: Deuterium lamp and tungsten lamp
• Wavelength range: 190 to 900 nm
• Wavelength accuracy: ± 1 nm
• Wavelength repeatability: y: ± 0.1 nm
• Optical bandwidth: 6 nm at 254 nm

Includes vacuum pump

Antek’s Model 8060 CLND uses Antek’s accurate and precise Pyrochemiluminescent technology to deliver an equimolar response for all nitrogen-containing compounds. The Chemiluminescence method is clean, fast and interference free. This groundbreaking method can determine all bound nitrogen in a variety of matrices.  Methods for characterizing crude products of a synthesis, quantitating target compounds or generating potency measurements may be based on traditional detectors, such as UV.  These methods are often inaccurate and time consuming. Additionally, this type of detector response depends on

chromophores and requires a purified  reference for each compound you investigate.  The Antek 8060 Series provides the solution. Our range of nitrogen-specific HPLC analyzers enables analysts to calculate compound potency using only a single, high purity nitrogen calibration standard.

Specifications:

• Detection: Pyro-chemiluminescence
• HPLC System Requirements: Operates on any system with an accurate pulse-free pump. Typical mobile phases (non-nitrogenous containing) include water, alcohol, TFA and other modifiers.
• Flow rate: 10–300 μL/min\
• Sensitivity: <0.1 nanogram of nitrogen
• Outputs: 1 mV, 1 V, 10 V
• Gases:
  • Dry oxygen: 50–200 cc/min 99.75% 4.5 bar (60 psig).
  • Dry carrier (argon or helium): 50–200 cc/min 99.99%, 4.5 bar (60 psig)
• Dimensions: 15cm x 26cm x 21cm (W x D x H) (6 x 10.23 x 8.27 inches)
• Weight: 36 kg (80 lbs)

Specifications:

• Maximum flow rate: 10 ml/min per channel
• Number of channels: 4
• Internal volume per channel: Typically 12 ml per channel
• Materials in contact with solvent: PTFE, PEEK
• pH range: 1 – 14
• Analog output (AUX): For pressure monitoring, range 0 – 3 V

Specifications:

• Flow rate range: 1-10,000 µL/min
• Flow rate accuracy: ±0.1% at 1 mL/min
• Flow rate precision: <0.05% RSD or <0.01 min SD, whichever is greater
• Pressure range: 2-50 MPa (7250 psi)
• Pressure ripple: Typical <0.2 MPa or <1%, whichever is greater

The Thermo Scientific Dionex UltiMate 3000 FLD-3000 Fluorescence Detector series provides high-sensitivity fluorescence detection for UltiMate 3000 HPLC systems. It is available in rapid separation (200 Hz) and standard (100 Hz) versions. The optics of the FLD-3000 Fluorescence Detector series provide maximum stray light suppression for unrivaled detection sensitivity. Innovative technologies supply various tools for individual method development, such as 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition. Operated with Thermo Scientific Dionex Chromeleon Chromatography Data System software, these detectors provide automated qualification, performance optimization, and instrument wellness monitoring for ease of use, maximum uptime, and the highest degree of regulatory compliance.

• Data collection at up to 200 Hz for optimal support of even the shortest UHPLC separations
• Lowest limits of detection with a Raman S/N: > 550 ASTM (> 2100 using dark signal as noise reference)
• Unsurpassed reproducibility with active flow cell temperature control for stable fluorophore activity independent from changes in ambient temperature
• Long-life xenon flash lamp for highest sensitivity and long-term operation without the need for frequent lamp changing
• Optional second photomultiplier for unique Dual-PMT operation offering an extended wavelength range up to 900 nm without sacrificing sensitivity in the standard wavelength range
• 2D or 3D excitation, emission, or synchro scans provide the highest degree in flexibility for method development or routine sample characterization
• Innovative variable emission filter for real-time, compound-related sensitivity optimization (FLD-3400RS only)
• Large front panel display for easy monitoring of detector status
• Flow cell syringe injection kit as a cost-effective solution to perform off-line measurements
• Optimized flow cell design ideal for trace analysis in combination with narrow-bore UHPLC columns and conventional HPLC columns
• Full regulatory compliance with automated qualification period monitoring and automatic tracking of flow cells

Specifications:

• Light Source: Xenon Flash Lamp
• Lamp Pulse Frequency:
  • HighPower (~300 Hz)
  • Standard (~100 Hz)
  • LongLife (~20 Hz)
• Data Collection Rate:
  • Single wavelength: up to 200 Hz (FLD-3400RS)
  • Multiple wavelength: up to 4 Hz (FLD-3400RS)
  • Single wavelength: up to 100 Hz (FLD-3100)
• Spectrum Scanning Modes: 2D or 3D excitation, emission or synchro scans (3D scans under Chromeleon 7.1 control)
• Wavelength Range:   
  • Variant  Ex Min   Ex Max  Em Min Em Max
  • 3100     200       630       265       650
  • 3400RS 200       630       220       650
  • 3100 with Dual-PMT       200       880       265       900
  • 3400RS with Dual-PMT   200       880       220       900
• Spectral Bandwidth:
  • Excitation: 20 nm
  • Emission: 20 nm
• Emission Filter:          Variable, 5 positions (FLD-3400RS) Fixed, 280 nm (FLD-3100)
• of Fluorescence Channels: Up to 4 (FLD-3400RS) 1 (FLD-3100)
• Excitation/Emission Wavelength Switching Time: < 250 ms
• Wavelength Accuracy: ±2 nm
• Wavelength Repeatability: ±0.2 nm
• Sensitivity: Raman S/N: > 550 ASTM over the entire lifetime of the lamp (> 2100 using dark signal as noise reference)
• Flow Cell Thermostatting: Ambient + 10 °C to 50 °C
• Flow Cell:
  • Analytical flow cell volume: 8 μL
  • 20 bar (290 psi) maximum cell pressure
  • Micro flow cell volume: 2 μL
  • 40 bar (580 psi) maximum cell pressure
• PC Connection:           All functions controllable through USB
• GLP Features: Automatic Equipment Qualification (AutoQ), System Wellness, and Qualification Monitoring with Chromeleon, cell ID chip
• Dimensions (h × w × d):
  • 16 × 42 × 51 cm
  • (6.3 × 16.5 × 20 in.)
• Weight: 18 kg

The Thermo Scientific Dionex UltiMate 3000 FLD-3000 Fluorescence Detector series provides high-sensitivity fluorescence detection for UltiMate 3000 HPLC systems. It is available in rapid separation (200 Hz) and standard (100 Hz) versions. The optics of the FLD-3000 Fluorescence Detector series provide maximum stray light suppression for unrivaled detection sensitivity. Innovative technologies supply various tools for individual method development, such as 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition. Operated with Thermo Scientific Dionex Chromeleon Chromatography Data System software, these detectors provide automated qualification, performance optimization, and instrument wellness monitoring for ease of use, maximum uptime, and the highest degree of regulatory compliance.

• Data collection at up to 200 Hz for optimal support of even the shortest UHPLC separations
• Lowest limits of detection with a Raman S/N: > 550 ASTM (> 2100 using dark signal as noise reference)
• Unsurpassed reproducibility with active flow cell temperature control for stable fluorophore activity independent from changes in ambient temperature
• Long-life xenon flash lamp for highest sensitivity and long-term operation without the need for frequent lamp changing
• Optional second photomultiplier for unique Dual-PMT operation offering an extended wavelength range up to 900 nm without sacrificing sensitivity in the standard wavelength range
• 2D or 3D excitation, emission, or synchro scans provide the highest degree in flexibility for method development or routine sample characterization
• Innovative variable emission filter for real-time, compound-related sensitivity optimization (FLD-3400RS only)
• Large front panel display for easy monitoring of detector status
• Flow cell syringe injection kit as a cost-effective solution to perform off-line measurements
• Optimized flow cell design ideal for trace analysis in combination with narrow-bore UHPLC columns and conventional HPLC columns
• Full regulatory compliance with automated qualification period monitoring and automatic tracking of flow cells

Specifications:

• Light Source: Xenon Flash Lamp
• Lamp Pulse Frequency:
  • HighPower (~300 Hz)
  • Standard (~100 Hz)
  • LongLife (~20 Hz)
• Data Collection Rate:
  • Single wavelength: up to 200 Hz (FLD-3400RS)
  • Multiple wavelength: up to 4 Hz (FLD-3400RS)
  • Single wavelength: up to 100 Hz (FLD-3100)
• Spectrum Scanning Modes: 2D or 3D excitation, emission or synchro scans (3D scans under Chromeleon 7.1 control)
• Wavelength Range:   
  • Variant  Ex Min   Ex Max  Em Min Em Max
  • 3100     200       630       265       650
  • 3400RS 200       630       220       650
  • 3100 with Dual-PMT       200       880       265       900
  • 3400RS with Dual-PMT   200       880       220       900
• Spectral Bandwidth:
  • Excitation: 20 nm
  • Emission: 20 nm
• Emission Filter:          Variable, 5 positions (FLD-3400RS) Fixed, 280 nm (FLD-3100)
• of Fluorescence Channels: Up to 4 (FLD-3400RS) 1 (FLD-3100)
• Excitation/Emission Wavelength Switching Time: < 250 ms
• Wavelength Accuracy: ±2 nm
• Wavelength Repeatability: ±0.2 nm
• Sensitivity: Raman S/N: > 550 ASTM over the entire lifetime of the lamp (> 2100 using dark signal as noise reference)
• Flow Cell Thermostatting: Ambient + 10 °C to 50 °C
• Flow Cell:
  • Analytical flow cell volume: 8 μL
  • 20 bar (290 psi) maximum cell pressure
  • Micro flow cell volume: 2 μL
  • 40 bar (580 psi) maximum cell pressure
• PC Connection:           All functions controllable through USB
• GLP Features: Automatic Equipment Qualification (AutoQ), System Wellness, and Qualification Monitoring with Chromeleon, cell ID chip
• Dimensions (h × w × d):
  • 16 × 42 × 51 cm
  • (6.3 × 16.5 × 20 in.)
• Weight: 18 kg

The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations:

Features:

• Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons
• Non-circular drive gears provide smooth, uniform eluent delivery
• User-programmable flow rate calibration and compressibility compensation factors ensure straightforward pump operation qualification
• Tests to retention time variations of less than 0.1% for highly reproducible peak performance
• Easy-to-read display and keypad for error-free programming
• Automatic purging and flow calibration

Specifications:

• Flow rate precision 0.1%, accuracy 1%
• Flow rate up to10.000 mL/min, in 0.001-mL/min increments
• Maximum operating pressure: 6000 psi