Used Lab Equipment

The Agilent 1200 Series autosampler is designed for use with other modules of the Agilent 1200 Series LC system or with other LC systems if adequate remote control inputs and outputs are available. The autosampler is controlled from the Agilent 1200 Series control module or from the Agilent ChemStation

for LC systems.

Specifications:

• Weight: 15.5 kg (34.2 lbs)
• Dimensions (height × width × depth): 200 × 345 × 440 mm (8 × 13.5 × 17 inches)
• Line voltage: 100 – 240 VAC, ±10 % Wide-ranging capability
• Line frequency: 50 or 60 Hz, ±5 %
• Power consumption (apparent power): 300 VA Maximum
• Power consumption (active power): 200 W Maximum
• Ambient operating temperature: 4 to 55 8C (41 to 131 8F)
• Ambient non-operating temperature: -40 to 70 8C (-4 to 158 8F)
• Humidity: < 95 %, at 25 to 40 8C (77 to 104 8F) Non-condensing
• Operating Altitude: Up to 2000 m (6500 ft)
• Non-operating altitude: Up to 4600 m (14950 ft) For storing the autosampler

Comprises a 4-channel vacuum container, including 4 tubular plastic membranes, and a vacuum pump.

Specifications:

• Maximum flow rate:
  • 10 ml/min per channel
  • 5-10 ml/min per at reduced degassing performance
• Number of channels: 4
• Internal volume per channel: Typically 12 ml per channel
• Materials in contact with solvent: PTFE, PEEK
• pH range: 1 – 14
• Analog output (AUX): For pressure monitoring, range 0 – 3 V

Can be sold as an individual component or as part of a complete stack

This Agilent 1200 Model G2226A ( G 2226A, G 2226 A, G2226 A ) Series Nano (NanoFlow) Pump is in excellent condition!  The Agilent 1200 Series nanoflow pump uses a unique solvent delivery technology optimized for nanoliter-per-minute flows and features EFC (Electronic Flow Control) with active feedback and real time flow adjustment. EFC is the gold standard for robust and reliable nanoflow performance and delivers the precise flow and gradient performance necessary for retention time reproducibility and stable ion generation essential for high sensitivity nanospray MS performance.

Features:

• Electronic Flow Control (EFC) provides real-time adjustment of the flow rate throughout the analysis for reliable and reproducible results.
• Micro vacuum degassing with very low internal volume per channel (1 mL) for fast changeover of mobile phase and quick purge.
• Control from handheld controller for convenient local status monitoring.
• 2nd generation flow sensors.
• Application focus: High resolution separations and high sensitivity MS detection. Proteomics is a typical application area for the nanoflow pump.

Specifications:

• Pressure: Operating range 0 – 40 MPa (0 – 400 bar, 0 – 5800psi)
• GLP features: Early maintenance feedback (EMF), electronic records of maintenance and errors
• Communications: Controller-area network (CAN). GPIB (IEEE-448), RS232C, APG-remote standard, optional four external contact closures and BCD vial number output
• Safety features: Leak detection and safe leak handling, low voltages in maintenance areas, error detection and display
• Injection range:
  • 900 µl in 0.1 µl increments (recommended 1 µl increments)
  • Up to 1800 µl with multiple draw (hardware modification required)
  • Up to 5000 µl with multiple draw (hardware modification required)
• Replicate injections: – 99 from one vial
• Precision:
  • Typically < 0.5% RSD of peak areas from 5 – 2000 µl,
  • Typically < 1% RSD of peak areas from 2000 – 5000 µl
  • Typically < 3% RSD of peak areas from 1 – 5 µl
• Minimum sample volume:
  • µl from 5 µl sample in 100 µl microvial
  • 1 µl from 10 µl sample in 300 µl microvial
• Sample viscosity range: 0.2 – 50 cp
• Sample capacity:
  • 100 × 2-ml vials in 1 tray
  • 15 × 6-ml vials in ½ tray (Agilent vials only)
• Injection cycle time: Typically 50 s, depending on draw speed and injection volume

Agilent 1200 Series - G4208A

The Instant Pilot provides complete local control and monitoring of a single module or an entire Agilent 1100/1200/1260 and 1290 Series HPLC system. There is no data evaluation in the Instant Pilot. The Instant Pilot allows you to do a variety of HPLC tasks including automated sample preparation and injection, isocratic, gradient and multiple method analyses and basic diagnostics and maintenance.

Specifications:

• Dimensions (W x D x H): 130 × 225 × 35 mm (5.1 × 8.9 × 1.4 inches)
• Weight: 0.8 kg (1.76 lbs)
• Line Voltage: 22 VDC, ± 10 %
• Power Consumption: 7 W
• Ambient operating temperature: 4 - 55 °C (39 - 131 °F)
• Ambient non-operating temperature: -40 – 70 °C (-40 – 158 °F)
• Humidity: < 95%, at 25 – 40 °C (77 – 104 °F)
• Operating Altitude: Up to 3000 m (9842 ft)
• Non-Operating Altitude: Up to 4600 m (15092 ft)

Features:

This Agilent 1220 Infinity LC - G4288B has a Gradient Pump, Manual injector, Variable wavelength detector configuration.


Specifications:

Weight: 43 kg (94 lbs)
Dimensions (height × width× depth): 640×370×485 mm (25.2×14.6×19.1 inches)
Line frequency: 50 or 60 Hz, ± 5 %
Power consumption: 240 VA / 210 W / 717 BTU
Ambient operating temperature: 4–55 °C (39–131 °F)
Ambient non-operating temperature: -40 – 70 °C (-40 – 158 °F)
Humidity: < 95 % r.h. at 40 °C (104 °F)

Features:

This versatile and highly reliable pump offers flow rates up to 10 mL/min and pressures up to 600 bar. It is suited for the use of smaller particle size columns giving higher resolution and faster separations. It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual floating, precise servo-controlled pistons adjust the stroke volume according to your chosen flow rate. Offering access to up to four solvents, the 1260 Infinity II Quaternary Pump provides the greatest flexibility in automated solvent blending. It is recommended for a wide range of research and routine applications (especially in the food, environmental and pharmaceutical sectors) as well as for method development.

Specifications:

• Higher resolution, faster separations - the maximum pressure of 600 bar allows you to use smaller particle size columns, longer columns and/or higher viscous solvents as an alternative to acetonitrile.
• Analytical and semi-preparative purifications - the wide flow range (up to 10 mL/min) perfectly supports standard and semi-preparative applications. The perfect choice for 4.6 and 3.0 mm id columns.
• Quicker experiments - the pump offers convenient access to up to four solvents for isocratic or gradient analysis, which can really speed up method development, preparation of mobile phases and system flushing. 
• Easy, fast and secure transfer of methods - from conventional to sub-2-micron columns.
• Integrated degasser
• Longevity and low operating costs - robust materials (stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE) withstand the most demanding applications and keep your pump functioning for years. Our optional seal wash for high-salt mobile phases keeps maintenance to a minimum and reduces operating costs.

Features:
The Agilent 1260 Infinity Thermostatted Column Compartment provides stable column cooling and heating up to 80 °C for standard applications. It fits neatly in a single stack of 1260 Infinity modules for lowest bench space requirements. An optional 2-position/6-port switching valve is available for 600 bar operation. For more sophisticated applications the Agilent 1290 Infinity Thermostatted Column Compartment is available, which fits seamlessly into any 1260 Infinity system.

Specifications:
Peltier cooling and heating for maximum temperature stability and application flexibility.
Spacious column racks hold up to three full-length, 30 cm columns.
Optional 2-position/6-port switching valve for 600 bar operation.
Simultaneous column identification of two columns for faster operation and reduced laboratory costs.
Independent heat exchangers with settable values facilitate two different temperature zones.
Low internal volume contribution of just 9 µL for low dispersion.

Features:

The Agilent 1260 Infinity II Vialsampler is an autosampler designed for the reliability and ease-of-use needed for routine pharmaceutical tasks and quality control, as well as for environmental and food analyses. It can house optionally the integrated column compartment for two LC columns with temperature control up to 80 °C, as well as a sample thermostat for stable temperature control from 4 °C - 40 °C, all within one module.

Specifications:

• High capacity - up to 132 vials (2 mL) or up to 36 vials (6 mL).
• Reliable injections -  0.1 to 100 µL injections for up to 600 bar (G7129A) and up to 800 bar (G7129C).
• Easy volume extensions- for injection volumes up to 1,500 µL (G7129A), or up to 1,800 µL (G7129C) for applications ranging from microbore to semi-preparative chromatography.
• Lowest carryover - with an in needle flush port included, for rinsing the outside of the needle.
• Efficient temperature control - with an integrated column compartment as an option or upgrade available. Which holds two columns up to 30 cm length, and provides heating capacity from 5 degrees above ambient up to 80 °C for reproducible chromatography data at optimized resolution.
• Integrated sample thermostat- available as an option or upgrade, providing cooling and heating (with thermostat) in the range from  4 °C - 40 °C.
• Low internal volume - for minimum contribution to a system's total internal volume, which can be even further reduced using bypass mode.
• Increased productivity - with overlapped injections.
• Customizable injection program - available for customizing advanced injections as well as for sample preparation steps upfront injection.

Features:

The Agilent 1260 Infinity II Diode Array Detector WR offers multiple wavelengths and full spectral detection at up to120 Hz sampling rate, keeping up with the analysis speed of fast LC. Even at high data rates, the 1260 Infinity II DAD WR enables precise identification, quantification, and peak purity analysis at trace levels. Maximum flexibility is guaranteed with a wide wavelength range of 190 to 950 nm with dual-lamp design, as well as the choice of 12 analytical, preparative and SFC flow cells.

Specifications:

• Fast data acquisition - full spectral data acquisition rate of 120 Hz increases resolution and delivers fast, highly sensitive peak purity analysis and spectral confirmation – at trace levels.
• Highest sensitivity - dual lamp design allows analysis of wavelengths between190 to 950 nm.
• Simultaneous data acquisition - The diode array design enables simultaneous acquisition of up to eight compound-specific wavelengths for increased sensitivity and selectivity.
• Rapid optimization of sensitivity, linearity and spectral resolution-programmable slit (1 to 16 nm) provides optimum incident light conditions.
• Eliminates background interference- with reference wavelengths.
• Minimized short-term noise (< ± 7 µAU ASTM) - low noise front-end electronics and special flow cell design deliver lowest detection limits.
• Maximum flexibility, compatibility and investment protection – with a range of 12 analytical, preparative and SFC flow cells.

.

The Agilent Technologies 1200 Series (G1310B) 1260 Infinity Isocratic Pump is a HPLC workhorse for demanding QA/QC and routine applications, designed to provide virtually pulse-free and stable solvent flow. This delivery principle allows variable stroke volume optimized to selected flow rate. The broadened pressure range with maximum pressure of 600 bar allows the use of small particle size columns, longer columns, or alternative solvents of higher viscosity. Additionally, the G1310B is the ideal pump for GPC/SEC applications where run-to-run and day-to-day precision of retention times are important.

Specifications:

• Settable flow range: set points 0.001 - 5 mL/min, in 0.001 mL/min increments
• Flow range: 0.2 to 10.0 mL/min
• Flow precision: ≤ 0.07 % RSD or ≤ 0.02 min SD
• Flow accuracy: ± 1 % or 10 μL/min whatever is greater, pumping degassed H2O at 10 MPa (100 bar)
• Pressure operating range:
  • Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min
  • Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min

1260 Infinity - G1311B 

The quaternary pump comprises an optional solvent cabinet, a vacuum degasser

 and a four- channel gradient pump. The four- channel gradient 

pump comprises a high- speed proportioning valve and a pump assembly. 

It provides gradient generation by low pressure mixing. A solvent cabinet 

provides enough space for four one- liter bottles. An active seal wash 

(optional) is available when the quaternary pump is used with 

concentrated buffer solution 

Specifications:  

• Hydraulic System: Dual piston in series pump with servo-controlled variable stroke drive, 
• power transmission by gears and ball screws, floating pistons 
• Setable Flow Range: Set points 0.001 – 10 mL/min, in 0.001 mL/min increments 
• Flow Range: 0.2 – 10.0 mL/min 
• Flow Precision: ≤ 0.07 % RSD, or ≤ 0.02 min SD whatever is greater, based on retention time at constant room temperature 
• Flow Accuracy: ±1 % or 10 µL/min whatever is greater, pumping degassed H2O at 10 MPa (100 bar) 
• Pressure Operating Range: Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min, Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min 
• Pressure Pulsation: < 2 % amplitude (typically < 1.3 %), or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar,147 psi) 

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack

Features:

The detector is designed for highest optical performance, GLP compliance and easy maintenance. 

Specifications:

• Detection Type: 1024-element photodiode array
• Light Source: Deuterium and tungsten lamps The UV-lamp is equipped with RFID tag that holds lamp typical information.
• Data Rate: up to 80 Hz
• Wavelength Range: 190 – 950 nm
• Short Term: noise (ASTM) Single and Multi-Wavelength: < ± 0.7·10-5 AU at 254 and 750 nm
• Drift: < 0.9·10-3 AU/h at 254 nm 
• Linear Absorbance Range: > 2 AU (5 %) at 265 nm
• Wavelength Accuracy: ± 1 nm ,Self-calibration with deuterium lines, verification with holmium oxide filter
• Wavelength Bunching: 1 – 400 nm, Programmable in steps of 1 nm
• Slit Width: 1, 2, 4 , 8, 16 nm Programmable slit
• Diode Width: < 1 nm

1260 Infinity - G1322A 

The Agilent 1260 Infinity Standard Degasser G1322A comprises a 4- channel vacuum container, including 4 tubular plastic membranes, and a vacuum pump.

Specifications: 

• Maximum Flow Rate: 10 mL/min per channel 
• Number of Channels: 4 
• Internal Volume Per Channel: Typically 12 mL per channel 
• Materials in Contact With Solvent: PTFE, PEEK 
• pH Range: 1 - 14 
• Analog Output (AUX): For pressure monitoring, range 0 – 3 V