Used Lab Equipment

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Minigel System Features

• Ideal for quick screening
• Uses 7 x 10cm gel tray with two comb positions
• Runs up to 16 samples on one gel with combs supplied
• Provides easy, leak-free casting without tape, grease or seals

Specifications

• Gel Dimension: 7 x 10cm
• Sample Size: 16 samples on one gel
• Buffer volume: 400mL

This power supply offers multiple choices for operating modes, either constant voltage or constant current output, with automatic crossover between modes. The automatic shutoff saves times, energy and reduces errors. Automatic recovery eliminates the need to restart the unit.

Specifications:

• Amperage: 400 mA

• Timer: 0 - 999 minutes

• Voltage: 300 V

The Mini Gel Tank is compatible with a variety of Novex mini gels, NuPAGE mini gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used.

Specifcations:

• Capacity: Up to 2 gels per run
• Gel Compatibility: Bolt Bis-Tris Plus Gels, Novex Mini Gels

The GE Healthcare Electrophoresis Power Supply EPS 3501 XL is a high quality, high precision and safe power supply for electrophoresis applications that require advanced programming and high voltage. EPS 3501 XL is primarily designed for techniques using programming in several phases and/or voltage gradients.

Specifications:

• Output Range: : 0–3 500 V DC, 0–400 mA,  0–200 W
• Programming Range: 35–3 500 V DC, 1–400 mA, 1–200 W
• Output Resolution: 1V, 1 μA, 0–399 μA
• Programming Resolution: 5V, 1mA, 1W
• Accuracy: 1%, +/-V; 1% +/- 1mA; 2% +/- 1W; 0.1% +/- 1 min
• Line Regulation:  < 0.2 %
• Load Regulation: < 1% at load change 10-90% of max load
• Ripple: < 1% at 3500V
• Short Term Stability: < 0.2%/year
• Long Term Stability: <1%/year
• Ambient Operating Temperature: 4 - 40C
• Ambient Operating Humidity: 0 - 95%
• Ambient Operating Pressure: 84 - 106 kPa
• Dimensions: 315 x 250 x 95 mm
• Weight: 3.8 kg

The revolutionary iBlot Dry Blotting System transfers proteins from polyacrylamide gels to membranes in seven minutes or less. The iBlot® western blotting system offers high transfer quality for downstream immunodetection sensitivities.

• Fast— protein transfer in 7 minutes or less
• High quality transfer— efficient transfers without bubbles or distortions
• Convenient—Self contained unit without the need for additional buffer or external power supply
• Reproducible—reduces blot preparation and running variability

250 Volts and 500 milliAmps DC.  Two sets of outputs operate in parallel to provide between 20 and 250 volts, up to 500 milliamps, and up to 75 watts in the constant voltage mode.

Specifications:

• Voltage Steps, Lo Range: 20,30,40,50,60,70,80,90, I00, 110,120, 130
• Voltage Steps, Hi Range: 140,150,160,170,180,190,200,210,220,230,240,250
• Maximum Voltage: 250V
• Maximum Current: 300 mA at 150-250V, 500 mA below 150V
• Maximum Power: 75 watts
• Number of Outputs: 2
• Temperature Range: 0°C -40°C
• Dimensions: 6 x 8 x 4.5 in
• Weight: 5 lbs

Electrophoresis Power Supply

Specifications:

• Output Voltage: 500V (Direct Current)
• Output Current: 500 mA
• Output Power: 250W

The PowerPac 1000 power supply is perfect for most SDS-PAGE applications. In addition, its programmability makes it ideal for running isoelectric focusing, or experiments requiring step changes in electrophoresis conditions. With sufficient voltage for IEF and sufficient current for multiple PAGE gels, plus the control required for reproducibility, it is the ideal power supply for 2-D electrophoresis.

Specifications:

• Power Requirements: 1000V, 500 mA, 250W

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

Ideal for most applications including protein mini-gels, SDS-PAGE, and DNA electrophoresis.

Specifications:

• Temperature Range: 0 to 40 °C
• Dimensions: 10” D x 7.5” W x 4” H

Ideal for most applications including protein mini-gels, SDS-PAGE, and DNA electrophoresis.

Specifications:

• Temperature Range: 0 to 40 °C
• Dimensions: 10” D x 7.5” W x 4” H

Includes:

• Cooling Module
• Control Module
• Drive Module

Drive Module 

• Dimensions (W x D x H): 43 x 26 x 14 cm
• Construction: Aluminum chassis
• Weight: 8 kg (17.2 lb)
• Maximum voltage:250 V DC
• Maximum current: 500 mA
• Electrode potentials:Dynamically regulated (feedback adjustment) +/- 0.5%
• Input voltage range:
  • 90–132 VAC/47–63 Hz/0.7 A (maximum)
  • 198–264 VAC/47–63 Hz/0.35 A (maximum)
• Fuses:
  • 5 A Fast Blow for high voltage output (front panel)
  • A Slow-Blow (100/120 V) or
  • 5 A Slow-Blow (220/240 V)

Control Module          

• Dimensions (W x D x H): 31 x 13.5 x 17 cm
• Maximum current: 500 mA
• Allowable voltage gradients:6–6 V/cm, in 0.1 V/cm increments
• Data entry: Keyboard
• Power supply: 250 V maximum, to allow maximum gradient of 6 V/cm, continuously adjustable

Cooling Module

• Chassis: Aluminum
• Dimensions (W x L x H):23 x 42 x 24 cm
• Weight: 14 kg (30.1 lb)
• Compressor:1/12 HP
• Refrigerant:CFC 12
• Heat exchanger:Stainless steel
• Operating range: 5°C–25°C
• Cooling rate (pre-cool):75°C/min from ambient to 14°C
• Cooling capacity (input power): 75 W at 14°C
• Input voltage range       
  • 90–132 VAC/47–63 Hz/4 A
  • 198–264 VAC/47–63 Hz/2 A
• Fuses   
• 3 A Slow-Blow (100/120 V) or
• 0 A Slow-Blow (220/240 V)