Used Lab Equipment

Description:

The Ettan IPGphor II from GE Healthcare is the ideal option for obtaining perfectly resolved first dimensions of 2D gels. The manufacturer claims that the system provides ‘reproducibility, reliability, speed, accuracy, high protein loading capacity, and high throughput’, and in my experience, the system really stands up to their claim. To begin with, the system is very compact and can be housed on a tabletop in any corner of the lab. It is perfectly silent and occupies little space. The system requires an ambient, dust-free operating environment; however, we keep the instrument in a well air-conditioned room. The system contains gold-plated copper electrodes that provide the necessary contact to the 1D strips for carrying out isoelectric focusing. A very high voltage is applied between the electrodes and, therefore, caution should be used while handling the instrument. As a safety feature, the electrodes are automatically switched off when anyone opens the lid of the instrument.

Specifications:

• Timer control:
• Dimensions: 0-3 hr, fully adjustable
• Weight: 18 lbs (8 kg)
• Inner dimensions: 20 x 20 cm
• Gel capacity: 4 mini(8 x 7cm) gels, 2 criterion (13.3 x 8.7cm) gels, 1 large (20 x 20cm) gel (per frame)

Description:

The new Gene Pulser II system is a completely redesigned electroporation system, which offers improved performance, flexibility, sample protection, and safety. The Gene Pulser II system uses the Pulse Trac waveform delivery system to generate the most accurate exponential decay pulses possible for optimal cell transformation within an electroporation cuvette. The Pulse Trac delivery system accurately calculates the time constant of each pulse based on what is actually delivered to the sample.

Specifications:

• Input voltage: 100/120 V RMS, 50/60 Hz
• Input current: 15 amp RMS
• Maximum output voltage and current: 2,500 V at 125 amp peak (normal load); limited to 1,500 A during arc.
• Output waveform: Pulse Trac exponential decay with RC time constant dependent on sample and capacitor selected
• Output voltage adjustment: 50–2,500 V range (depending on the capacitor) with 10 V adjustment precision for the high voltage range and 2 V for the low-voltage range (50–500 V).
• Ambient operating environment: Temperature 0–35°C
• Humidity: 0–95% without condensation
• Regulatory: Passes requirements of IEC 1010. In addition, the system passes requirements for FCC, Class A.
• Dimensions: 34 x 31 x 19 cm
• Weight: 10.41 kg

Includes

• Gene Pulser II
• Capacitance Extender Plus
• Pulse Controller Plus

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

The revolutionary iBlot Dry Blotting System transfers proteins from polyacrylamide gels to membranes in seven minutes or less. The iBlot® western blotting system offers high transfer quality for downstream immunodetection sensitivities.

• Fast— protein transfer in 7 minutes or less
• High quality transfer— efficient transfers without bubbles or distortions
• Convenient—Self contained unit without the need for additional buffer or external power supply
• Reproducible—reduces blot preparation and running variability

The Mini Gel Tank is compatible with a variety of Novex mini gels, NuPAGE mini gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used.

Specifcations:

• Capacity: Up to 2 gels per run
• Gel Compatibility: Bolt Bis-Tris Plus Gels, Novex Mini Gels

The Accuris myGel Mini electrophoresis system includes all of the equipment that you need to get up and running - gel tank, power supply and two casting sets. Ideal for personal use, small laboratories or the classroom, this gel box/power supply combination is compact and easy to use. The myGel mini gel box attaches directly to the power supply for a secure connection every time. Selectable parameters include 3 preset voltages - 35V, 50V or 100V - and a 99 minute timer. All are set using the membrane keypad. The safety lid is vented to help dissipate heat generated during electrophoresis, and features a viewing area above the gel. Current will only run through the system when the lid is properly in place on top of the gel box.

Specification:

• Output Voltage: 35V, 50V or 100V
• Timer: 0-99 min. or Cont.
• Safety: Lid interlock
• Max. Buffer (ml): 230
• Gel Capacity: 10.5 x 6cm Two: 5 x 6 cm
• Weight: 1 lbs / 0.45 kg
• Dimensions: 7.5 x 5.1 x 2.1 in
• Electrical: 115V or 230V, 50-60Hz

The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can run 1–12 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the isoelectric focusing tray is independently powered, enabling precise control over each IPG strip. This feature makes it possible to run different sample types, different pH gradients, and multiple protocols simultaneously.

Specifications:

• User Interface: Display QVGA resolution (320 x 240) touch screen or mouse control
• Programmable: Yes
• Ramping: Step, linear, gradual, and hold voltage ramping for each focusing step. Hold mode as a final step to prevent diffusion when IEF is complete
• Protocol Capacity: 2 GB
• Data collection: .dat format
• Dimensions:3 in x 13.6 in x 15.1 in
• Weight: 19 lbs
• Input power: 100–240 VAC, 50/60 Hz

Description:

The Pulsewave 760 switcher is a versatile and safe instrument for pulsed field DNA electrophoresis. The unit may be used with Orthogonal Field Alternating Gel Electrophoresis (OFAGE), Clamped Homogeneous Electric Fields, and Field-Inversion Gel Electrophoresis (FIGE). The Pulsewave 760 switcher interfaces with an external power supply and gel box.

Specifications:

• Switch Intervals: 0.1 to 65,000 sec
• Forward/Backward Ratio: 0.1 to 99.9:, continuously adjustable, with 0.1 resolution
• Overload and Short Protection: Fuse and electronic protection circuitry
• Operating Temperature: 0-35°C
• Operating Humidity: 0-95%
• Dimensions (W x L x H): 6.5 x 11.5 x 6.5 in.
• Footprint: 486 sq. in.
• Weight: 20 lbs.

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9