Used Lab Equipment

The DSG-200-02 dual vertical system allows for two gels to be run simultaneously under identical buffer conditions. The cooling chamber assures constant temperature during electrophoresis. It also allows for two different gel casting options, the multi-caster method and the gel wrap casting system.

Specifications:

• Overall Dimensions: 24 x 23 x 29 cm (WxDxH)
• Glass Plate Dimensions: 5 x 22 cm
• Recommended Buffer Reservoir Volume: 300 mL upper, 125-200 mL lower

Electrophoresis Power Supply

Specifications:

Output Voltage: 500V (Direct Current)

Output Current: 500 mA

Output Power: 250W

The ECM 399 is an exponential decay wave electroporaton generator specifically designed to deliver the field strengths and pulse lengths required for the simple transformation of bacteria and yeast cells. In low voltage mode the ECM 399 has a limited capability for transfecting some mammalian cell lines.

Specifications:

• Power Requirements: 100 – 240VAC, 50/60Hz
• Operating Temperature: 10°C to 40°C
• Relative Humidity: 60%
• Altitude: 2000 m (operating)
• Dimensions:7 x 9.1 x 4.3 in
• Weight:2 kg (7 lbs)

The Pharmacia Electrophoresis Power Supply EPS 500/400 is an accurately controlled power source of modern design that has been designed for safe and easy operation. The EPS 500/400 is designed to be used with the Pharmacia electrophoresis apparatuses (eg. the Gel Electrophoresis Apparatusus GE 2/4 and GE 2/4 LS, and the Flat Bed Electrophoresis apparatuses FBE Immuno and FBE 3000) or with any other suitable electrophoresis or immunoelectrophoresis chambers.

The Power Supply EV265 has a powerful, easy to operate design that is small and compact. Storing of up to 9 methods is possible and allows for data logging of electrophoresis runs of about 100 hours.

Specifications:

• Voltage: 0 – 600 +/- 2%
• Maximum Current: 500 mA
• Maximum Output: 150W
• Timer: 0 -99, 59 h
• Minimum Load Resistor: 30 ohms
• Ambient Temperature: 0 - 40°C
• Dimensions: 24 x 20 x 13 cm
• Weight: 6 kg

Includes

• Gene Pulser II
• Capacitance Extender Plus

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications:

• Maximum Voltage: 3500 V (dc), ± 1750 V floating output with reference to ground
• Maximum Power: 100 W
• Max Pressure Cooling Plate: 0.35 bar
• Dimensions: 16 x 31 x 40 cm
• Weight: 4 kg
• Environment: 4ºC to 40ºC, 20% to 95% relative humidity