Used Lab Equipment

The MiSeq personal sequencing system delivers the fastest time to answer, a revolutionary workflow, and the widest breadth of sequencing applications, all in a compact and economical instrument. For results in hours, not days, MiSeq uses TruSeq, Illumina's reversible terminator-based sequencing by synthesis chemistry in combination with innovative engineering.

Features

• Go from sample to data in as little as 8 hours
• Prepare clusters, sequence, and analyze data on a single machine with walkaway automation.
• Footprint of less than 2 feet square, eliminating the need for auxiliary hardware and saving valuable lab bench space
• Flexible enough to handle a wide range of capillary electrophoresis and next-generation sequencing applications with the highest-quality data.

It's sequencing made accessible for every lab. Of every size. Every budget. It's MiSeq.

Specifications:

• Temperature range: 40–250°C
• Temperature increase: 2-5 °C/sec
• Pressure range: 0–20 bar (2 MPa; 290 PSI)
• Power range: 0–400 W at 2.45 GHz
• Reaction volumes: 0.2–20 mL
• Agitation: Variable magnetic stirrer (300-900 RPM)

Includes:

• Applied Biosystems 3730XL
• 96-Capillary
• Computer w/ Pre-loaded existing software
• Data Collections 3.0
• Warranty Period: 90 days
• Installation on site

Specifications 

• Laser: Argon-ion multi line, single mode laser: primary excitation lines 488 and 515.5nm
• Oven Temp: 18°C to 70°C
• Sample Volumes: 384 well plates = 5-30µl / 96 well plates = 10-50µl
• Plate Seal: Septa or Polypropylene heat seal
• Operating Environment: 18°C - 30°C, Humidity = 20 to 80%
• Capillary Array Lengths: 36 cm or 50 cm
• Capillary Dimensions: 150 5m o.d. / 50 5m i.d.
• Polymer Consumption
  • 36cm: approx. 200µl per run
  • 50cm: approx. 250µl per run

ABI 3730xl DNA Sequencer         

• Optics
  • In-capillary detection by dual-side illumination.
  • Multiple fiter sets: Z, E, G5, G5-RCT, Any4Dyeset, Any5Dyeset and S (optional)
  • Backside thinned CCD for maximum optical sensitivity
• Capillaries
  • Choice of capillary lengths, 36cm or 50cm
  • Dimensions: 150µm o.d./50µm i.d.
• Reagent Handling
  • Automated polymer replenishment pump for increase throughput
  • POP-7 performance robust polymer separation matrix
  • 5 sequencing run modules, 1 fragment analysis run and SNPlex run module
  • On-board reagents for up to 48-hours of unattended operation
• Performance Per Run Type  
  • LongSeq: 1152 samples per day (980 Kbases)
  • StdSeq: 2304 samples per day (1550 Kbases)
  • FastSeq: 2304 samples per day (1600 Kbases)
  • RapidSeq: 3840 samples per day (2100 Kbases)
  • GeneMapper Software
    • 4224 samples per day (92,000 genotypes)
    • 36 cm, up to 500 bp resolution with 0.15 bp sizing precision in 34 minutes

Power Requirements       

• Voltage: 200-220 volt or 230-240 volt

System Dimensions        

• Width 100 cm
• Depth 73 cm
• Height 89 cm
• Weight 180 kg