We offer an assortment of used and refurbished Thermal Cyclers ranging from Real Time PCR to qPCR, and Gradient Thermal Cyclers to non-Gradient Thermal Cyclers. Some of our popular manufacturers include ABI, MJ-Research, Bio-Rad, Agilent / Stratagene, and Eppendorf.
The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.
Alpha unit modules are available in several formats, including:
The DNA Thermal Cycler 480 automates the polymerase chain reaction (PCR) technique for amplifying DNA.
The GeneAmp PCR System 2400 has a strong performance history. To maintain the instrument in top working order it is advisable to perform routine maintenance on the instrument. Depending on the level of instrument usage, it is recommended that the following procedures be performed at least 2-4 times per year. For high end users a 3-month maintenance cycle is recommended and for lower end users a 6-month maintenance cycle is recommended.
GeneAmp PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications.
384 Well Block
The optical module houses the excitation system and the detection system. The Excitation system consists of a fan-cooled 50 watt tungsten halogen lamp a heat filter (infrared absorbing glass) a 6 position filter wheel fitted with optical filters and opaque filter "blanks" and dual mirror arrangement that allows simultaneous illumination of the entire sample plate.
The excitation system of the BioRad iCycler PCR Thermal Cycler is physically located on the right front corner of the optical module with the lamp shining from right to left perpendicular to the instrument axis. Light originates at the lamp passes through the heat filter and a selected color filter and is then reflected onto the plate in the thermal cycler by a set of mirrors. This light source excites the fluorescent molecules in the wells.
The Eppendorf Mastercycler gradient sets the standard in the modern molecular biology laboratory, with the Gradient PCR being the first-choice method for optimizing reaction conditions. A freely programmable temperature gradient of up to 20 °C enables the optimization not only of the annealing temperature, but also of all temperature steps of a PCR protocol for even the most demanding applications. Thanks to SteadySlope Technology, optimal, constant heating and cooling rates are guaranteed, which means that results of gradient experiments can be transferred simply and precisely to routine applications.
Mastercycler ep gradient
Mastercycler ep Control Panel
Quantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables.
The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses.
The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction.
16 well MiniCycler
The MJ Research PTC-150 MiniCycler is a reliable easy-to-use thermal cycler, designed by field experts. Users can take advantage of the PTC 150's easy-to-read programming interface. The MJ Research thermal cycler can also perform programming tasks as quickly and hassle-free as possible. Instrument set-up and transportation can be done with ease as well with the MiniCycler's space-saving design. Other notable capabilities of the PTC-150 are the Instant Incubate feature for continuous-temperature incubations and oil-free cycling as a result of using a Hot Bonnet heated lid.