Used Lab Equipment

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

The DNA Thermal Cycler 480 automates the polymerase chain reaction (PCR) technique for amplifying DNA.

Specifications:

• Capacity: 48 x 0.5 mL
• Temperature Range: -5°C to 100°C

Specifications:

• Capacity: 2 sets of 30 x 0.5 mL tubes

• Temperature Range: 0 - 105°C

• Temperature Accuracy: +/- 0.3°C

• Temperature Uniformity: +/- 0.4°C

• Ramping Speed: up to 3°C/sec

• Dimensions: 12 x 12 x 12 in

• Weight: 25 lbs

• Power Requirements: 100 - 240V, 50/60Hz, 850W

• Lowest Programmable Temperature: 30°C

Description:

The GeneAmp PCR System 2400 has a strong performance history. To maintain the instrument in top working order it is advisable to perform routine maintenance on the instrument. Depending on the level of instrument usage, it is recommended that the following procedures be performed at least 2-4 times per year. For high end users a 3-month maintenance cycle is recommended and for lower end users a 6-month maintenance cycle is recommended.

Description:

• Reproducibility Of Cycle Times
• Uniformity Of PCR Yields For Samples
• Thin-Walled MicroAmp Reaction Tubes Allowing Efficient Heat
• Transfer and Improving Equilibration Times
• High Speed of Heating and Cooling
• Heated Cover Assuring That Tubes Fit Tightly In The Wells
• Oil-Free Operation

Specifications:

• Dimensions: 30.5 x 52 x 61 cm
• Weight: 40.5 kg
• Input: 120 VAC, 60 Hz
• Display: 40 Character Blue Luminescent Display
• Temperature Range: 4°C to 99.9°C

GeneAmp PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications.

Includes:

• Manuals
• Power Cord

Specifications:

• Temperature Accuracy: ± 0.25 from 35.0°C - 99.9° C
• Method Storage: Auto restart function allows for power outages and safe continuation of a PCR experiment after resumption of power
• Modified Programs: Complete PCR methods can be stored with both a user and method names and protected from unitentional modification
• Geneamp 9700 Base Module: The system stores up to 100 complete PCR methods (pre-PCR holds, PCR cycling condition and post PCR holds)
• Internal Calibration: 4°C - 99.9°C
• User Diagnostics: All system software operations are controlled with five diagnostic programs

384 Well Block

The optical module houses the excitation system and the detection system. The Excitation system consists of a fan-cooled 50 watt tungsten halogen lamp a heat filter (infrared absorbing glass) a 6 position filter wheel fitted with optical filters and opaque filter "blanks" and dual mirror arrangement that allows simultaneous illumination of the entire sample plate.

The excitation system of the BioRad iCycler PCR Thermal Cycler is physically located on the right front corner of the optical module with the lamp shining from right to left perpendicular to the instrument axis. Light originates at the lamp passes through the heat filter and a selected color filter and is then reflected onto the plate in the thermal cycler by a set of mirrors. This light source excites the fluorescent molecules in the wells.

Features:

• Unique modular design for maximum flexibility and sensitivity
• Methods that enable a choice of PCR chemistry
• Outstanding thermal performance from the iCycler thermal cycler
• Gradient functionality for rapid assay optimization
• Easy-to-use software for efficient accurate data analysis
• Compatibility with outstanding PCR supermixes that provide optimum performance in real-time PCR assays

Specifications:

• Block configuration: 96
• Temperature Control: 4°C to 99°C
• Gradient Block: No
• Block Homogeneity: 20°C to 72°C ≤±0.4°C; 95°C≤±0.5°C
• Control accuracy: ±0.2°C
• Heating rate: ca. 3°C/s
• Cooling rate: ca. 2°C/s
• Dimensions (W x D x H): 10.4 x 16.4 x 10.8 in
• Weight: 27 lbs
• Power Supply: 120V, 50/60 Hz
• Number of programs: 100 on device; approx. 10 on personal card
• Max. number of cycles: 99
• Power consumption: 500 W

The Eppendorf Mastercycler gradient sets the standard in the modern molecular biology laboratory, with the Gradient PCR being the first-choice method for optimizing reaction conditions. A freely programmable temperature gradient of up to 20 °C enables the optimization not only of the annealing temperature, but also of all temperature steps of a PCR protocol for even the most demanding applications. Thanks to SteadySlope Technology, optimal, constant heating and cooling rates are guaranteed, which means that results of gradient experiments can be transferred simply and precisely to routine applications.

Mastercycler ep gradient

• Freely programmable temperature gradient over 12 rows
• Gradient range of up to 20°C
• Robust, specially coated aluminum block

Mastercycler ep Control Panel

• Highest degree of flexibility
• Intuitive graphic programming
• Variable ramp rates
• Emulation mode for temperature control speed and protocols of the Mastercycler family
• Time and temperature increments
• Control up to 5 thermomodules of the Mastercycler ep system. Features include pause command, programmable alarm sound and freely configurable auto-restart dialing. Windows-like user administration offers a clear overview, even with a large number of users and more than 700 saved PCR programs. To increase the 16 MB memory capacity, a separate MultiMediaCard is available.

Specifications:

• Block configuration: 96
• Temperature Control: 4°C to 99°C
• Gradient Block: Yes
• Block Homogeneity: 20°C to 72°C ≤±0.4°C; 95°C≤±0.5°C
• Control accuracy: ±0.2°C
• Heating rate: ca. 3°C/s
• Cooling rate: ca. 2°C/s
• Dimensions (W x D x H): 10.4 x 16.4 x 10.8 in
• Weight: 27 lbs
• Power Supply: 120V, 50/60 Hz
• Number of programs: 100 on device; approx. 10 on personal card
• Max. number of cycles: 99
• Power consumption: 500 W

Includes:

• Computer
• Software
• All Necessary Cables
• Manuals

Quantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables.

The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses.

The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction.

Applications

• DNA target quantification
• Gene expression
• SNP detection, Allele discrimination, GenotypingDNA methylation, Apoptosis
• Viral load assays, Pathogen detection
• Clinical diagnosis (Cancer therapy response)

16 well MiniCycler

The MJ Research PTC-150 MiniCycler is a reliable easy-to-use thermal cycler, designed by field experts. Users can take advantage of the PTC 150's easy-to-read programming interface. The MJ Research thermal cycler can also perform programming tasks as quickly and hassle-free as possible. Instrument set-up and transportation can be done with ease as well with the MiniCycler's space-saving design. Other notable capabilities of the PTC-150 are the Instant Incubate feature for continuous-temperature incubations and oil-free cycling as a result of using a Hot Bonnet heated lid.

Specifications:

• Thermal Range: -9Ëš to 105ËšC
• Accuracy: ±0.3ËšC of programmed target at 90ËšC, NIST traceable
• Thermal Homogeneity:
  • ±0.4ËšC well-to-well within 12 seconds of arrival at 90ËšC
  • ±0.3 ËšC well-to-well within 40 seconds of arrival at 90 ËšC
• Sample Capacity: 16-well block: 16 x 0.5mL tubes
• 25-well block: 25 x 0.2mL tubes or one 25-well microplate
• Line Voltage: 100-240VAC rms
• (no adjustment needed among voltages within these ranges)
• Frequency: 50-60Hz single phase
• Power: 250W maximum
• Fuses: Two T2.5A/250V
• Displays: One 2 x 16 LCD alphanumeric display
• Ports: One 25-pin 8-bit parallel interface printer port (optional)
• Memory: 80 typical programs, in non-volatile memory
• *each program may have up to 100 steps; maximum of 400 steps among all programs
• Weight: 3.0 kg
• Size: 22 x 8 x 11 cm high