Used Lab Equipment

BD FACS Aria II Sorp 5 laser instrument with a BioBubble. Robotic device for sorting into multi-well plates, large nozzle, temperature control, and Whispr.

Specifications:

488-nm laser

• 515/20-nm FITC
• 710/50-nm PerCP-Cy5-5 or PI

640-nm laser

• 660/20-nm APC
• 710/50-nm Alexa Fluor 700
• 730/45-nm AAPC-Cy7

405-nm laser

• 450/50-nm Pacific Blue
• 525/50-nm AmCyan
• 585/42-nm Pacific Orange
• 605/40-nm Qdot 605
• 660/40-nm Qdot 655
• 705/70-nm Qdot 705

532-nm laser

• 575/25-nm PE
• 610/20-nm PE-Texas Red
• 660/20-nm PE-Cy5
• 710/50-nm PE-Cy5-5
• 780/40-nm PE-Cy7

355-nm laser

• 450/50-nm Hoechst Blue, DAPI
• 525/50- Indo-1 AM

Includes PM From BD and Guaranteed Installable under BD Service Contract

New Flow Cell installed in September 2018

Also included are the excitation laser lines, laser powers and optical configuration.

The new BD FACSAria IIu cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria IIu cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria IIu cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cellsorting.

Includes:

• 4 lasers (375nm, 488nm, 405nm, 633nm)
• Blue 7PMTs, Red 3PMTs, Violet 3PMTs
• HP Computer w/ FACSDiva 8.0 Software
• ACDU
• 1 HP monitor
• Fluidics Cart
• Temperature Control
• Lauda Chiller
• Buffalo Whisper Aerosol Control
• Under BD service contract since installed in 2012

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

The BD FACSMicroCount system offers a comprehensive solution for rapid enumeration of microbes. The integrated system consists of an instrument and reagents to support quality manufacturing processes.

Specifications:

• Product Screening
  • Assay Format: Qualitative
  • Sample Volume: 1 mL default
  • Throughput/Hour: 20 samples
  • Reagents: Up to 2
  • Reagent Volumes: 200 μL Nucleic Acid Dye 100 μL BRAG3
  • Reaction Time/Reagent: 90 seconds
• Water Analysis
  • Assay Format: Qualitative
  • Sample Volume: Variable volume range 0.10–0.50 mL
  • Throughput/Hour: 12–15 samples
  • Reagents: Up to 3
  • Reagent Volumes: 100 μL Nucleic Acid Dye 100 μL BRAG3 Buffer, 100 μL
  • Reaction Time/Reagent: 175 seconds
• Microfermentation
  • Assay Format: Qualitative
  • Sample Volume: Variable volume range 0.10–0.50 mL
  • Throughput/Hour: 12–15 samples
  • Reagents: Up to 3
  • Reagent Volumes: Variable ranges- method specified
  • Reaction Time/Reagent: Method specified