Used Lab Equipment

Used Lab Equipment

We offer an assortment of used and refurbished Flow Cytometers and Cell Sorters. Some of our popular manufacturers include Beckman-Coulter, BD Biosciences (Becton-Dickinson) and Guava.

Benchtop Incubator Shaker: 406-420 of 446

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Wave Biotech BASE 2050EH Bioreactor

Wave Biotech BASE 2050EH Bioreactor

Cambridge ID #: 17883

Features:

  • Disposable Presterile Bioreactor Chamber
  • Fit Cellbags: 2x2L, 2x10L, 1x20L with KIT20 or 2x22L, 1X50L with KIT50
  • Completely closed system
  • Operate inside an incubator or on the benchtop

 

Includes:

  • Electric Rocker Base for 20/50EHT
  • Digital temperature control

 

Applications:

 

Monoclonal antibodies

The WAVE Bioreactor system has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scale up without transfers. Batches ranging from 100ml to 500L have been run with cell densities over 10x106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.

 

Anchorage-dependent cells

Agitation in the WAVE Bioreactor system is powerful enough to mix and aeratethe culture. Yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents setting and provides oxygenation without bubbles.

 

Virus production

The WAVE Bioreactor system provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4x106 cells/ml and virus production was 1x105 virus particles/cell. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet.

 

Insect cell / Baculovirus

The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.

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Benchmark Scientific BeadBug D1030 *NEW* Homogenizer

Benchmark Scientific BeadBug D1030 *NEW* Homogenizer

Cambridge ID #: 18229

The BeadBug is a high energy benchtop homogenizer that sets the performance standard for personal sized cell disruption and lysis instruments. Simultaneous homogenization of up to 3 samples takes place inside the disposable 2 ml screw cap microtubes. The optimize mixing motion causes rapid cell disruption through constant high velocity impact from the hardened micro-beads chosen specifically for the sample type. With up to three tube capacity and a footprint of just 7 x 8”, the BeadBug is the ideal homogenizing instrument for low to medium throughput laboratories.

 

Specification:

 

  • Speed Range: 2800 to 4000rpm
  • Max. Capacity: 3 x 2.0mL
  • Timer: 3 sec. to 3 min.
  • Operating Temp. Range: +4C to +65C
  • Dimensions: 6.9: x 8.3" x 5.3"
  • Weight: 5lbs
  • Electrical: 115V
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BioTek Biostack Automated Microplate Stacker

BioTek Biostack Automated Microplate Stacker

Cambridge ID #: 17138

Performance

  • Restacking feature allows plates to be returned to the input stack in their original order
  • Can be operated without an external computer when used with the ELx405 Microplate Washer, MicroFill Microplate Dispenser, NanoQuot Microplate Dispenser, or MicroFlo Select Microplate Dispenser
  • 30 or 50 standard height microplates (96- or 384-well) per interchangeable stack Optional rotational wrist allows users to integrate both landscape and portrait orientation microplate carriers with the instrument remaining in its optimal, ergonomic-friendly position
  • Variable microplate input Small footprint conserves precious benchtop space; fits easily in most standard laboratory hoods

 

Design

  • Universal design compatible with most supported BioTek instrumentation Designed for heavy use with aluminum precision-ground base Linear mechanisms built around proven BioTek technology Quiet operation with cams activated by stepper motors No moving parts to wear within motors Gripper incorporates rack and pinion drive for synchronistic movement Reliable transport with microplate gripped from below Onboard self-diagnostics ensure trouble-free operation

 

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BioTek BioStack 2 Automated Microplate Stacker

BioTek BioStack 2 Automated Microplate Stacker

Cambridge ID #: 15606

Specifications:

 

Performance:

  • Restacking feature allows plates to be returned to the input stack in their original order
  • Can be operated without an external computer when used with the ELx405 Microplate Washer, MicroFill Microplate Dispenser, NanoQuot Microplate Dispenser, or MicroFlo Select Microplate Dispenser
  • 30 or 50 standard height microplates (96- or 384-well) per interchangeable stack Optional rotational wrist allows users to integrate both landscape and portrait orientation microplate carriers with the instrument remaining in its optimal, ergonomic-friendly position
  • Variable microplate input Small footprint conserves precious benchtop space; fits easily in most standard laboratory hoods

 

Design:

  • Universal design compatible with most supported BioTek instrumentation Designed for heavy use with aluminum precision-ground base Linear mechanisms built around proven BioTek technology Quiet operation with cams activated by stepper motors No moving parts to wear within motors Gripper incorporates rack and pinion drive for synchronistic movement Reliable transport with microplate gripped from below Onboard self-diagnostics ensure trouble-free operation

Features:

  • <10 seconds plate exchange time
  • Range of formats including 24-, 48-, 96-, 384- and 1536-well
  • 10, 30, or 50 standard height microplates per interchangeable stack
  • De-lidding and re-lidding capability with patented BioStack 4
  • Rotational gripper for ergonomic  and compact integration
  • Restacking capability
  • Optional barcode scanner
  • Direct control through washer and dispenser interface or via LHC or Gen5 PC software
  • Small footprint conserves precious bench top space
  • Walk away automation
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Thermo Cellomics CellInsight Gel Imaging SystemImage Coming Soon

Thermo Cellomics CellInsight Gel Imaging System

Cambridge ID #: 10067

Description:

High-content screening (HCS) or high-content analysis (HCA) requires an instrument designed to extract the maximum information from your sample in a robust and reproducible manner with the speed for high-throughput analysis. The entire Thermo Scientific portfolio of HCA instruments helps deliver on those parameters and includes:

  • Superior illumination—the LED light engine reduces intensity fluctuations and optical component wear, eliminates moving parts, and reduces both integration and channel switching times
  • Rapid data analysis—Thermo Scientific HCS Studio software analyzes your images in real time, so that you collect all the data you need to make decisions that count
  • Reagent compatibility—Thermo Scientific HCS platforms build on the expertise of Molecular Probes in reagent development with exquisite fluorescent tools for interrogating the biology of the cell

Specifications:

  • Camera: Photometrics X1 Camera with 4.54um/pixel
  • Live cell: Optional HCA On-Stage Incubator with Kinetic scheduling of acquisition
  • Software: HCA Studio 3.1 Cell Analysis Software. Optional client software available
  • Database: STORE Express Image and Database Management Software Included, Scalable SQL/Oracle database options available
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Microzone Clean Cell HLF-2-4 Laminar Flow HoodImage Coming Soon

Microzone Clean Cell HLF-2-4 Laminar Flow Hood

Cambridge ID #: 6451

Description:

These cost-effective vertical laminar flow and horizontal laminar flow workstations feature the Clean-Ceil fan filter module, which utilizes a HEPA filter that is 99.99% efficient for all particles >=0.3µm. The workstations may be used as either benchtop or console units. They incorporate a modular design approach that allows the user to customize a workstation by choosing the optional accessories necessary to meet individual requirements. The workstations come complete with aluminum support frame, clear Plexiglas side panels, 40% efficient ASHRAE-rated prefilter, white plastic laminate back panel (vertical model only, filter is on top), or clear Plexiglas top panel (horizontal model only, filter is on back).

Specifications:

  • Dimensions (D x H x W): 21” x 22” x 46”
  • Electrical: 115 V, 60 Hz
  • Air velocity: 90 ±20 fpm
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Biospherix Medical Closed Isolator System Laminar Flow Hood

Biospherix Medical Closed Isolator System Laminar Flow Hood

Cambridge ID #: 19843

Features:

  • The Bioshperix is a completely closed isolotar system with incubators that is capable to producing an ISO 5 environment.
  • The complete incubator system has 4 modules each with a length of 57 ¼” by 30 5/8” for a total length of 332.37” and depth of 119.47”.
  • The systems also includes 2, 36” by 24” Laminar flow hoods at each end of the Isolator for introducing materials intop the closed environment.
  • Each module is spearated from each other by means of a buffer box.
  • One module of the isolator has 2 enclosed incubators capable fo growing and processing cells. Each incubator has outside dimensions of 21.25” W × 33”D × 50.5” H.
  • The incubator system also includes a leak testing panel to test for leaks in the system to demostrate compliance with FDA requirements.
  • The unit also has 2 chillers to cool the chambers to 4°C. Two separate set of chillers are included one to cool the working surface and other to cool the enviromnet if so desired.
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Invitrogen Countess  Automated Cell Counter

Invitrogen Countess Automated Cell Counter

Cambridge ID #: 20134

Features:

 

The Countess™ automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.

 

Specifications:

 

  • User-friendly, benchtop design for simple, fast, automated cell count and viability measurements within a minute  
  • Provides data on cell size and is compatible with a wide variety of eukaryotic cells without the need for any special changes between large or small sizes  
  • Measures cell concentrations ranging from 1 × 104 to 1 × 107 cells/mL and cells with sizes ranging from 5 μm to 60 μm 
  • Uses disposable counting chamber slides that eliminate washing steps and cross contamination between samples  
  • Saves and prints cell count data including images using the Countess™ software and Countess™ USB drive  
  • Presents comprehensive data with graphical reports and as a .CSV (comma separated value) file for sample comparisons  
  • Allows you to gate and analyze cells based on cell size 
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Shandon Cytospin 4 Cytocentrifuge

Shandon Cytospin 4 Cytocentrifuge

Cambridge ID #: 8871
"

Gain all the advantages of the ultimate thin-layer cell preparation system with the Thermo Scientific Cytospin 4 Cytocentrifuge. This reliable benchtop centrifuge provides economical thin-layer preparations from any liquid matrix, especially hypocellular fluids such as spinal fluid and urine.

Specifications:

  • Dimensions: 16 x 9.5 x 24.5 in
  • Weight: 26.5 lb
  • Power Requirements: 100-240V, 50/60Hz, 1Ph
  • Capacity: 12 specimens at a time
  • Speed Displays: 200 - 2000 rpm
"
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Biocare Medical DC2002 Decloaking Chamber

Biocare Medical DC2002 Decloaking Chamber

Cambridge Scientific ID #'s: 18555, 18553

The Decloaking Chamber is a programmable benchtop pressure cooker intended for laboratory use. It is programmed to allow the precise pressurized heating necessary for antigen retrieval and also has the capability to perform at a variety of temperatures ranging from 37 °C to 125 °C. The Decloaking Chamber is an excellent tool for heat-induced epitope-retrieval (HIER) methods. The proper use of heat and pressure in conjunction with the appropriate buffer solutions is of the utmost importance for consistent immunohistochemistry (IHC) staining. The Decloaking Chamber is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety requirements. Temperature, pressure and pH can be monitored and recorded with the Decloaking Chamber to produce consistent staining, which will reduce repeat testing and false negatives.

 

Specification:

 

  • Dimensions (W x D x H): 31 x 29 x 28
  • Pan Capacity: 3.2 liters
  • Weight: 3.7 (8.18 lbs)
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Guava EasyCyte Flow Cytometer

Guava EasyCyte Flow Cytometer

Cambridge ID #: 16054

Single Laser Configuration

 

Includes Computer Loaded with Cytosoft Software

 

The guava easyCyte flow cytometry systems are cost-effective, easy to use and deliver complete and comprehensive cell analysis- right on your benchtop. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers—all while providing the analytical power you need to expand your research horizons.

 

Features:

  • Blue Laser
  • Forward and Side Scatter
  • Tube Sampling
  • Automated 96 Well Micrplate Sampling
  • Small Sample Size (<20 μL)
  • Minimal waste (<50 mL per day)
  • Detectors
    • Green
    • Yellow
    • Red

 

Fluidics Specifications:

  • Flow Cell Dimension: Standard square capillary with ID of 100 µm
  • Pump: Positive displacement
  • Sheath Fluid: None
  • Sample Flow Rate: 7µL/min to 72 µL/min
  • Waste Generation: typically <40mL in 8 hours of continuous use
  • Dead Volume: 50 µL (for 96-well microplate)
  • Sample Requirement: as few as 2,000 cells/test; typically 25,000 – 100,000 cells
  • Cell Size: 1-60 µm
  • Typical # of Cells/Protocol: 1,000 – 10,000 cells/test
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Perkin Elmer EnVision 2104-0020 Multilabel Microplate Reader

Perkin Elmer EnVision 2104-0020 Multilabel Microplate Reader

Cambridge ID #: 17876

Includes:

  • Bar Code reader
  • Computer loaded with Software

 

 

The 2103 EnVision Multilabel Plate Readers are fast, sensitive and versatile benchtop readers that deliver optimized performance in every application and for every label. Their unique design features modular label-specific optical mirror modules, high energy flash lamps, and high speed detectors.

Specifications:

  • Light Source: Xenon flash lamp
  • Plate Formats: 1 to 3456
  • Temperature Control:
    • Temperature range: from 2 °C above ambient up to 45 °C (0.5 °C increments)
    • Uniformity: ± 1 °C
    • Heating time: < 10 minutes (from RT up to 37 °C)
  • Shaking: linear, orbital, dual orbital
  • Dispenser:
    • Dispense volume: 2-475 μL (1 μL increments)
    • Pump speed: 100-500 μL/s
    • Dead volume: 750 μL. With pump-back function < 50 μL using stardard tubing length and factory settings.
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BD BioScience FacsCanto II - 3 Laser Flow Cytometer

BD BioScience FacsCanto II - 3 Laser Flow Cytometer

Cambridge ID #: 12160

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

 

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

 

Includes:

  • Computer w/ FacsDiva Software
  • Fluids Cart
  • 2 laser, Blue(488) and Red (633)
  • Laser
  • 6 PMTs unit and side scatter
  • All Necessary Cables
  • Manuals

 

Optics Specifications:

  • Lasers
    • Air-cooled:
    • 488-nm solid state, 20-mW laser output
    • 633-nm HeNe, 17-mW laser output
  • Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
  • Optical Alignment Procedure: Fixed, no operator alignment required
  • Flow Cell: 180 x 430-μm rectangular quartz flow cell
  • Collection Optics: Optical-gel coupled 1.2 NA lens
  • FSC Resolution: 0 μm
  • SSC Resolution: 5 μm
  • Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
  • FSC Detector: Photodiode with 488/10 BP
  • SSC Detector: PMT with 488/10 BP
  • Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
  • Blue Laser Dyes
    • FITC, PE, PerCP or PerCP-Cy™5.5,
    • PE-Cy7 (525, 575, 678 or 695, 785 nm)
  • Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
  • Detector Bands
    • Blue Laser: 530/30; 585/42; >670; 780/60 nm
    • Red Laser: 660/20; 780/60 nm
  • Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF
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BD BioScience FACSCanto II 2 Laser Flow Cytometer

BD BioScience FACSCanto II 2 Laser Flow Cytometer

Cambridge ID #: 17183

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

 

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

 

Includes:

  • Computer w/ FacsDiva Software
  • Fluids Cart
  • 2 laser, Blue(488) and Red (633)
  • 3 PMTs in 4-2 configuration w/ side scatter
  • All Necessary Cables
  • Manuals

 

Optics

Lasers

  • Air-cooled
  • 488-nm solid state, 20-mW laser output
  • 633-nm HeNe, 17-mW laser output
  • Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
  • Optical Alignment Procedure: Fixed, no operator alignment required
  • Flow Cell: 180 x 430-μm rectangular quartz flow cell
  • Collection Optics: Optical-gel coupled 1.2 NA lens
  • FSC Resolution: 1.0 μm
  • SSC Resolution: 0.5 μm
  • Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
  • FSC Detector: Photodiode with 488/10 BP
  • SSC Detector: PMT with 488/10 BP
  • Fluorescence Detectors: 6 PMTs in 4-2 configuration w/ side scatter
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BD BioScience FACSCanto II 3 Laser Flow Cytometer

BD BioScience FACSCanto II 3 Laser Flow Cytometer

Cambridge ID #: 18798

Description:

Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high-quality results. The BD FACSCanto II can be configured with two or three lasers to detect up to eight colors. It features many innovations, including a true fixed alignment flow cell to minimize startup time and improve reproducibility. The optical system maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay. These and other capabilities make the BD FACSCanto II ideal for today’s busy clinical lab, providing a high degree of automation and quality control. With optimal reproducibility, the BD FACSCanto II system reduces hands-on technician time and costs associated with repeat testing.

Specifications:

  • Carryover: ≤0.1%
  • Sample Injection: Direct into flow cell
  • Max Particle Size: 50 μm
  • Sample Flow Rate, Min: 10 μL/min
  • Sample Flow Rate, Max: 120 μL/min
  • Sample Acquisition Rate: 10,000 events/second, 6 compensated fluorescence parameters and 2 scatter parameters
  • Sample Dead Volume: 30 μL
  • Sheath Consumption: 1.10 L/h normal operation; <1 mL/h standby
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