Used Lab Equipment

SENTERRA Raman Microscope combines numerous novel and patented features such as fluorescence removal and on-demand confocal depth profiling. The most important innovation is certainly its continuous calibration method, as it eliminates daily calibrations with external standards, providing you reliable, reproducible measurements.

Specifications:

• High wavenumber accuracy with SureCal
• All-in-one, compact, confocal design
• Removal of fluorescence background with Concave Rubberband Correction
• Confocal depth profiling with FlexFocus
• Up to 4 excitation lasers
• Computer controlled XYZ mapping stages with autofocus

Bruker AMX-300 Nuclear Magnetic Resonance Spectrometer with the "piano" style console and an unshielded Oxford 300MHz magnet. The magnet was de-energized about five years ago. All of the kits for moving the magnet are included.  

This is a 2-channel system with a solids accessory rack with high power 1H and X transmitters. Three probes included (plus a Doty solid state probe): a 5mm 1H inverse probe, a 10mm BB, and a standard bore magic angle solids probe (7 & 4mm stators).  

This Bruker BioSpin Avance III HD 400 High Performance Digital NMR Spectrometer was never installed and is unused in storage.

Specifications:

Frequency Controller & Signal Generation Unit

• Basic configuration two channels frequency range each channel: 5–650 MHz each
• Number of numerically controlled oscillators per channel: 3
• Frequency resolution: < 0.005 Hz
• Phase resolution: < 0.006º
• Attenuation resolution / attenuation range: < 0.1 dB / 90 dB
• Amplitude modulation: > 90 dB
• Event time for digital setting of frequency, phase and amplitude, either individually or simultaneously: 25 ns
• Memory per channel e.g. for pulse shaping: >8 Mbyte
• Phase monotony over 90 dB amplitude setting range: < + / - 0.5 º

Timing Controller

• Pulse sequence parameter calculation: in real time
• Timing resolution / min. duration:5 / 25 ns
• Output bus for events and devices: 96-bit

Communication Protocol

• Fast Ethernet TCP / IP

Host Computer

• Operating system: Windows 7

Receiver:

• Bandwidth: up to 5 MHz

Digital Receiver Unit (DRU)

• Effective dynamic range of digitizer
• SW < 5 MHz: > 16-bit
• SW < 1 MHz: > 18-bit
• SW < 6 kHz: > 22-bit
• Data compression / digital filtering: on the fly
• Optimum real time digital filters: Yes

Quadrature Receiver (RXAD):

• Quadrature image peaks with digital quadrature detection: 0%

Features:

• Advanced temperature controlled detector optics ready to use within two hours of installation
• Automatic zero and automatic purge combined with a recycle valve for automatic solvent recycling allow uninterrupted operation
• Automatic light intensity control circuit to ensure the optimum performance of the optics
• Integrated diagnostics for efficient troubleshooting
• Built-in refractive index calibration
• Front access to valves and capillaries for easy maintenance
•  

Specifications:

• Detection type: Refractive Index
• Refractive index range: 1.00 – 1.75 RIU, calibrated
• Measurement range: +/- 600 x 10-6 RIU
• Optics temperature control: 5 ° C above ambient to 55 ° C
• Sample cell volume: 8uL
• Maximum pressure: 5 bar (0.5Mpa)
• Maximum flow rate: 5mL/minute
• Valves: Automatic purge and automatic solvent recycle
• Volumes: Inlet port to sample cell 62uL, inlet port to outlet port 590uL

The Waters 2475 Fluorescence Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag. The 2475 FLR Detector sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths, and has the ability to monitor up to four independent wavelength pairs.

Features:

• Innovative axially illuminated flow cell designed for less dispersion, less stray light, less volume, and more pathlength for maximum sensitivity
• Three-dimensional spectral scanning for faster method development and optimization, and enhanced peak identification
• Single or multi-channel operation monitors fluorescence at one or more discrete wavelength pairs
• Low noise performance - Less stray light due to orthogonal optics and the use of mirrors, not lenses, minimize scatter
• Multiple detection modes - 2D, 3D, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence detection methods by quickly determining wavelength maxima
• Integral erbium calibration reference – ensures wavelength accuracy
• Normalized emission units for better bench-to-bench reproducibility

Model 3300 ELSD is designed for use with High Performance Liquid Chromatography (HPLC) systems to analyze any sample compound that has sufficiently lower volatility than the mobile phase. Some of its possible application areas include carbohydrates, pharmaceuticals, lipids, triglycerides, underivatized fatty and amino acids, polymers, surfactants, nutraceuticals, and combinatorial libraries. Evaporative light scattering detection eliminates common problems associated with other HPLC detectors. Refractive Index (RI) detectors can be complicated by solvent front interferences, baseline instability, and gradient incompatibility. RI detectors can also have a less sensitive response than ELSD. Low-wavelength UV can suffer baseline drift with steep gradients and also requires that the analyte contains a chromophore. ELSD does not have these limitations. It can achieve stable baselines with multisolvent gradients for improved resolution and faster separations. Also, since ELSD response does not depend on the sample’s optical characteristics, the sample does not require a chromophore or fluorophore for detection.

Specification:

• Light Source: Laser diode with collimating optics, 650nm, max output less than 30mW, class IIIB
• Product Class: Class 1 laser product
• Detector Element: Silicon photodiode
• Temperature Range: Ambient to 120°C in 0.1°C increments
• Nebulizer Gas: Up to 4L/min, nitrogen preferred, 65psig min. pressure, 80psig max pressure
• Mobile Phase Flow Rate: 50uL - 3.0mL/min
• Analog Outputs: Selectable for either 0 - 1V or 0 - 10mV full scale

• Communications

• Remote Inputs: TTL/Contact closure-Autozero, Gas Shutoff, Start, Standby
• Outputs: Contact closure-- Fault Relay, External Output; RS232, USB (Device), USB (Host), Ethernet
• Operating Parameter Selection & Display: Windows-Based Graphical LCD with alphanumeric keypad
• Power Requirements: 120/240V, 50/60Hz, 5A
• Dimensions: 11.6" H x 10.3" W x 19.5" D (29.5cm H x 26.2cm W x 49.5cm D)
• Weight: 30 lbs (13.6 kg)
• Environmental Operating Temperature: 15 to 40ËšC
• Relative Humidity: 10 - 90%, non-condensing

A revolutionary design packed with features, the AE2000 sets a new benchmark in affordable inverted microscopy.

Motic’s new AE2000 Inverted Microscope is designed for routine live cell inspection with an easy and flexible set up, ideal for University, routine-clinical, and lab environments. The model’s main applications offer superior image quality when viewing cell cultures in microbiology, living organisms and tissue culture samples.

The newly designed Binocular Ergo tubes with 360° swiveling “butterfly” design offers the most comfortable observation position, especially useful for prolonged sample viewing. The auto on/off function of the power supply extends the lifespan of the sample and light bulb and at the same time saves energy. Another added feature, a modular interchangeable illumination concept, allows for the easy transition from Halogen to LED to fulfill a wide-range of customer viewing expectations. The improved phase contrast quality of the model makes the instrument suitable for a greater range of applications that demand precision optics.

The entire microscope including optical system is manufactured now completely Lead Free, following the International RoHS regulations.

Features:

• AE2000 Binocular Inverted Microscope with PL PH 20x
• Binocular head Siedentopf type 45° inclined
• Widefield high eyepoint eyepiece 10X / 20mm (paired), focusable
• Side facing quadruple nosepiece
• CCIS Plan Achromat objective PL PH4x, PL PH 10x, PL PH 20x
• ELWD N.A. 0.30 condenser
• Phase slider Ph0, BF, Ph1
• Centering telescope
• Plain Stage with metal & glass stage inserts
• Halogen illumination 6V/30W with intensity control
• 45mm blue, Green Interference and Diffusion filters with filter slider
• Allen hexagonal key, vinyl dust cover, power cord
• Universal Power Supply 100-240V

A revolutionary design packed with features, the AE2000 sets a new benchmark in affordable inverted microscopy.

Motic’s new AE2000 Inverted Microscope is designed for routine live cell inspection with an easy and flexible set up, ideal for University, routine-clinical, and lab environments. The model’s main applications offer superior image quality when viewing cell cultures in microbiology, living organisms and tissue culture samples.

The newly designed Binocular Ergo tubes with 360° swiveling “butterfly” design offers the most comfortable observation position, especially useful for prolonged sample viewing. The auto on/off function of the power supply extends the lifespan of the sample and light bulb and at the same time saves energy. Another added feature, a modular interchangeable illumination concept, allows for the easy transition from Halogen to LED to fulfill a wide-range of customer viewing expectations. The improved phase contrast quality of the model makes the instrument suitable for a greater range of applications that demand precision optics.

The entire microscope including optical system is manufactured now completely Lead Free, following the International RoHS regulations.

Features:

• AE2000 Binocular Inverted Microscope with PL PH 20x
• Binocular head Siedentopf type 45° inclined
• Widefield high eyepoint eyepiece 10X / 20mm (paired), focusable
• Side facing quadruple nosepiece
• CCIS Plan Achromat objective PL PH4x, PL PH 10x, PL PH 20x
• ELWD N.A. 0.30 condenser
• Phase slider Ph0, BF, Ph1
• Centering telescope
• Plain Stage with metal & glass stage inserts
• Halogen illumination 6V/30W with intensity control
• 45mm blue, Green Interference and Diffusion filters with filter slider
• Allen hexagonal key, vinyl dust cover, power cord
• Universal Power Supply 100-240V

Live Cell Microscope Platform

• Trinocular head Siedentopf type, 45^o inclined (Light split 100:0/20:80)
• Widefield high eyepoint eyepieces WFPL10X/22mm with diopter adjustment on both eyepieces and rubber eyecups
• Side facing quintuple nosepiece CCIS Plan achromatic objective PL 4X
• Coaxial coarse and fine focusing adjustment
• Hard coated stage with metal and glass stage inserts
• ELWD condenser N.A. 0.30 (WD 72mm)
• Phase slider Ph1 and brightfield
• Phase centering telescope
• Koehler illumination quartz halogen 6V/30W with external lamp house and intensity control
• Objectives
• CCIS Plan achromatic Phase objective PL Ph 10X, LWD
• CCIS Plan achromatic Phase objective PL Ph 20X
• Plan Achromat Phase LWD PL Ph1 40X, N.A. 0.5 / W.D. 3.0
• Filter Cubes
• DAPI/Hoechst/AlexaFluor 350
• EGFP/FITC/Cy2/AlexaFluor 488  
• TRITC/Cy3/TagRFP/AlexaFluor 546
• 100w Mercury Fluorescent light w/ power supply OR X-Cite 120LEDMini
• 54mm Petri dish holder
• 0.5X C-mount camera adapter for 1/2” chip sensors

Includes: X-Cite 120LED Head, miniCube, speedDIAL, microscope flange, accessory kit

Features

• LED Technology
• White Light Excitation
• Direct Coupled
• Multiple Control Options
• Small Footprint
• Optimized Thermal Management
• X-Cite Coupling Optics

Benefits

• Efficiency, stability, instant ON/OFF and long lifetime
• Excitation of commonly used fluorophores such as DAPI, CFP, GFP/FITC, YFP, mCherry/Texas Red, and Cy5
• High Power excitation
• Simple operation through USB, TTL or speedDIAL
• Minimal space requirement ; less bench top real estate used
• Maximum LED output, lifetime, stability and reliability
• Efficient optical output and uniformity with highest performing coupling optics

Specifications:

• Wavelength Range: 370-700nm
• External Power Supply: Universal input 100-240VAC, 50/60Hz
• Power Consumption: 150W
• LED ON/OFF Response Times: 85μs TTL / 1ms USB
• Control Options: ON/OFF - TTL compatible, speedDIAL, RS-232 commands (SDK available), USB
• I/O Connections: BNC input , USB (B-type)
• Dimensions – HEAD: 100mm x 135mm x 110mm (W x H x D)
• Dimensions – CONTROLLER: 180mm x 110mm x 230mm (W x H x D)
• Dimensions – speedDIAL: 80mm x 59mm x 112mm (W x H x D)
• Weight: 3.4 kg (7.5 lb)
• Certifications: RoHS, CE
• Warranty: LEDs – 20,000 hours, All other X-Cite 120LEDmini components - 1 year, parts and labor

Features:

The Motic BA310POL Trinocular with infinity optics is ideal for life sciences, geology, material science and forensics. It features strain-free CCIS optics built-up around a sturdy frame with centerable objectives, making it a dedicated, professional polarizing microscope.

Specifications:

• 30° inclined Trinocular head: for comfortable viewing while seated
• Light split: 100:0/0:100
• Interpupillary distance adjustment: between 55-75mm
• Widefield high eyepoint: N-WF10X/20mm, diopter adjustment on both eyepieces, rubber eyecup (paired), cross hair on one eyepiece
• Intermediate tube: with rotatable analyzer 360º and Bertrand lens, focusable
• Quartz wedge: full λ and 1/4λ retardation plates
• Reversed quadruple nosepiece: with click stops for precise magnification changes, individually centrable with one reference position
• CCIS EC Plan Strain-free objectives: EC PL 4X/0.10, 10X/0.25, 40X/0.65-Spring, 60X/0.80-Spring
• Coaxial coarse and fine focusing system: with 2 micron minimum increment with tension adjustment
• Vertical travel range: 20mm
• 360º circular rotating stage: with a diameter of 160mm, lockable position
• Focusable and centrable Achromat swing-out condenser N.A. 0.90/0.13: Strain-free Rotatable polarizer, fixed on condenser carrier
• Collector lens assembly: with screw-on filter holder
• Koehler illumination: quartz halogen 6V/30W with intensity control

Features:

The Bio-Plex 3D suspension array system is the next generation multiplexing platform based on xMAP technology. Expanded multiplexing capability, faster time to results, and automation capability make it the platform of choice for high-throughput testing for nucleic acid and protein applications.

Specifications:

• Software: xPONENT (acquisition) Bio-Plex Manager (analysis)
• Optics: Lasers/APDs/ PMTs
• Multiplex Capacity: 500
• Bead Compatibility: MagPlex-C, MicroPlex, LumAvidin, xTAG
• Read Time: ~20 min/96-well plate, ~75 min/384-well plate
• Applications: High-throughput
• Plate Format: 96-well/384-well
• Dynamic Range:5 logs

Includes: computer, touch screen monitor, manuals, and software.

Features:

• Multiple collection modes: Fluorescence, phosphorescence, chemi/bio-luminescence, and time resolved phosphorescence measurements can all be easily made without changing a single hardware component
• Lowest cost of ownership: The Cary Eclipse is so robust and reliable that every new instrument comes with a 10-year lamp life guarantee, reducing ongoing expenses to virtually zero. 
• Improved laboratory safety: Additionally the guaranteed exceptionally long lifetime of the lamp in the Cary Eclipse eliminates lamp replacements, a hugely time-consuming and potentially hazardous operation.
• Easy to use: Modular, application-specific software, which satisfies the needs of novice and expert users alike, and the ability to switch between measurement modes without touching the hardware, ensure that any user can quickly get reliable, accurate results with minimal training.
• Expand your analytical capabilities: The superior, unique design of the Cary Eclipse ensures room light immunity allowing the instrument to operate with the front and top panels removed for easy access to sample and fast installation of large accessories.
• Proven sensitivity where you need it most: Optimized design coupled with unique, high-sensitivity optics and detectors ensure that the Cary Eclipse gives you the performance that you need right across the spectrum. 
• Robustness, reliability and sensitivity: Engineered to be the most robust instrument on the market, the Cary Eclipse incorporates the unique Agilent xenon flash lamp to capture a data point every 12.5 ms and scans at 24,000 nm/min without peak shifts perfect for high throughput laboratories and fast kinetics applications.
• Unique Sampling: By incorporating Agilent’s unique capacity to guarantee room light immunity coupled with the highly accurate and reproducible Peltier temperature control, the Cary Eclipse delivers unique sampling opportunities that will expand your analytical capabilities, simplify your workflow and increase your sample throughput.
• Reliable Results: The Cary Eclipse only illuminates the sample when data is actually being acquired. This means that the Cary Eclipse prevents your valuable samples from photodegradation and preserves their integrity to the point that you can measure them over and over again and continue to get the same, reliable result. 

Specifications:

• Dimensions(WxDxH): 60 x 64 x 27 cm (23 x 25 x 10 inches)
• Sensitivity:> 500:1 RMS, 500 nm excitation, excitation and emission slits 10 nm / > 750:1 RMS, 350 nm excitation, excitation and emission slits 10 nm
• Wavelength(s):190 - 1100 nm
• Temperature Range: 10 – 35°C
• Optical System: Cerny-Turner 0.125 m Monochromators
• Light Source: Xenon Flash Lamp
• Software: Cary Eclipse Software

Features:

The second product in the EcoLine by Motic series is a digital microscope that can be viewed by more than one student at the same time. The bright and clear TFT screen mounted on the head of this glass-optics microscope serves as both a camera and a viewing platform. Capture still (5.0MP) images as well as Video onto the system's internal memory or onto an SD card that can be put into the microscope. The glass 4x, 10x and 40x(s) objectives provide clear images and adhere to normal educational standards. With the 4x objective, viewing of other specimens such as fabrics is made possible through the bright LED toplight, which is mounted on a flexible arm. This is a simple but versatile microscope packed in a hard carrying case along with sample microscope slides.

Specifications:

• Monitor: 3" TFT monitor
• Sensor: 5.0MP CMOS
• Still capture resolution (interpolation): 12.0MP maximum
• Memory: 64MB internal ,SD card slot
• Illumination: Top LED, Bottom LED, Dimming control
• Objectives: Coated Glass 4x, 10x, 40x(s)
• Carrying Case: Plastic

The EMax Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. A staggering mean time between failure (MTBF) of 20,000 hours is achieved by 97 independent and stationary fiber optics so that the microplate remains stationary during the entire reading cycle, reducing the number of moving parts. No optical alignment needed during the lifetime of the instrument!

Includes:

• Computer Loaded with SoftMax Pro 5.4.1
• All Necessary Cables and Cords
• Manuals

Features:

• Extend uptime with an estimated 20,000 hour mean time between failures
• Maximize assay signal-to-noise ratios through a unique optical system that directs all available light to each well
• Achieve higher throughput with a 5 second microplate read time
• Read up to 2 wavelengths in endpoint absorbance for 96-well plates
• Mix samples in microplate wells by user-programmable Automix feature
• Operate without an attached computer using the onboard microprocessor
• Add assay flexibility with additional filters

General Specifications:

• Dimensions (H x W x D):4 x 18.3 x 13.4 in.
• Wavelength range: 400 - 750 nm
• Photometric range:000 to 4.000 OD
• Wavelength selection: Filters
• Photometric resolution: 0.001 OD
• Endpoint read time: 5 seconds
• Temperature range: Ambient

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.