Used Lab Equipment

The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

Includes:

• Computer w/ Software
• Monitor
• Manuals

Product Features:

• Automation of the standard trypan blue assay
• % Viability
• Total cell concentration
• Total viable cell concentration
• Mean cell size
• Real time cellular images
• Bioprocess monitoring
• Validated reagents
• Convenient reagent pack

 The Vi-CELL XR offers additional benefits over the S and AS Models:

• Extended size range to 2 microns, allowing additional analyses of yeast, and smaller cells.
• Increased sample throughput with greater efficiency.
• Reduced sample volume results in less reagent use.
• Auto focus capability increases instrument ease of use.
• Variable aspiration and mixing cycles optimize results.
• Enhanced image magnification provides unparalleled cellular detail.
• Circularity measurement helps isolate debris from cells.

 Specifications:

• Analysis Time: < 2.5 min
• Capacity: Single or 12 position via autoloader
• Concentration Range: 5 x 10^4 to 1 x 10^7 cells/mL
• Counting Accuracy: ± 6%
• Depth: 41 cm (16 in)
• Digitizing Resolution: 1394 x 1040
• Height: 44.5 cm (17.5 in)
• Imaging Technology: Auto-focus CCD array, Firewire camera
• Instrument Type: Video image through a quartz flow cell
• Overall Analysis Range: Size Range: 2 µm - 70 µm
• Sample Volume: 0.5 mL
• Temperature: 10°C to 40°C (50°F to 104°F)
• Viability Range: 0 to 100%
• Weight: 11.3 kg (25 lb)
• Width: 38 cm (15 in)

Features:

The Muse Cell Analyzer delivers accurate assessments of cell health parameters, cell signaling activation pathways & immune status in just minutes, revolutionizing the way you analyze cells.

Specifications:

• Cell Number per Assay: User defined; cell concentrations of 10,000–500,000 cells/mL
• Sample Format: Single loader
• Sample Volume: 200 µL
• Emission Max: Yellow: 28nm bandwidth at 576nm, Red: 30nm bandwidth at 680nm, Forward scatter = laser wavelength
• Excitation Max: Green Laser (532nm)
• Scatter: Forward
• Throughput: Less than 2 minutes per sample
• Dimensions 8.69” H  x 8.12” W x 11.11” L

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

Features:

The YSI 2300 STAT PLUS Glucose and Lactate Analyzer is a laboratory instrument intended for use in clinical care and sports medicine applications. It provides quick measurements of glucose in whole blood, plasma or serum; and of L-lactate in whole blood, plasma, or cerebrospinal fluid (CSF). In whole blood or plasma, glucose and L-lactate can be measured simultaneously.

Specifications:

• Sample size: 25 microliters (aspirated volume)
• Response Time (from Test Tube Holder station):

Normal Mode: Displayed/printed result in 65 seconds

                           Sample to sample interval is 100 seconds

Screen Mode: Displayed/printed result in 45 seconds

                          Sample to sample interval is 70 seconds

• Linear Range:

 Normal Mode:

Glucose: to 50.0 mmol/L (900 mg/dL or 9000 mg/L)

               Lactate: to 30.0 mmol/L (267 mg/dL or 2670 mg/L)

 Screen Mode:

Glucose: to 27.8 mmol/L (500 mg/dL or 5000 mg/L)

Lactate: to 15.0 mmol/L (134 mg/dL or 1335 mg/L)

• Calibration point:

Glucose: 10.0 mmol/L; 180 mg/dL; 1800 mg/L

Lactate: 5.00 mmol/L; 45 mg/dL; 445 mg/L

• Enzyme membrane working life (typical):

Glucose: 21 days (YSI 2365)

               Lactate: 14 days (YSI 2329)

• Precision:

Glucose: ±2% of the reading or 2.5 mg/dL (25 mg/L, 0.2 mmol/L),whichever is larger

 Lactate: ±2% of the reading or 0.1 mmol/L (1 mg/dL, 10 mg/L), whichever is larger

• Resolution:

              Glucose: 1 mg/dL (1 mg/L, 0.1 mmol/L)

Lactate: 0.1 mmol/L (1 mg/L, 1 mg/dL)

Optional Xylose kit w/computer and Xylose software

 Includes:

• Turntable
• Free Starter Kit  
• Biochemistry analyzer for bioprocessing, food and beverage, and biofuels applications

It provides rapid, accurate analysis of key nutrients and byproducts, including:

• Glucose
• Lactate
• Glutamine
• Glutamate
• Ethanol
• Lactose
• Sucrose
• Hydrogen peroxide
• Methanol
• Starch
• Choline
• Xylose
• Galactose

Specifications:

• Aspirated sample volume: User
• Selects: 5 to 65 microliters
• Analysis time: 60 seconds
• Precision: <2% cv, n=10
• Linearity: 5%, calibration value to range maximum
• Includes: Turntable 2710

Optional Xylose kit w/computer and Xylose software

 Includes:

• Turntable
• Free Starter Kit  
• Biochemistry analyzer for bioprocessing, food and beverage, and biofuels applications

It provides rapid, accurate analysis of key nutrients and byproducts, including:

• Glucose
• Lactate
• Glutamine
• Glutamate
• Ethanol
• Lactose
• Sucrose
• Hydrogen peroxide
• Methanol
• Starch
• Choline
• Xylose
• Galactose

Specifications:

• Aspirated sample volume: User
• Selects: 5 to 65 microliters
• Analysis time: 60 seconds
• Precision: <2% cv, n=10
• Linearity: 5%, calibration value to range maximum
• Includes: Turntable 2710

Description:

FlowCam® is an imaging particle analysis system that uses flow microscopy to image and analyze subvisible particles with diameters ranging from 1 μm to 600 μm. Simultaneously determine particle shape, type, and size distribution of all detectable particles in your solution.

Specifications:

• Particle Size Range: 1 μm to 600 μm
• Magnification & FlowCells:
  • 20X (~200X magnification), flow cell depth option: 50 μm Field-of-View (FOV)
  • 10X (~100X magnification), flow cell depth option: 100 μm FOV
  • 4X (~40X magnification), flow cell depth option: 300 μm and 600 μm FOV
• Sample Processing Capability: 0.05 mL/minute at 20X and up to 3mL/minute at 4X
• Camera: High resolution (1920x1200 pixels) CMOS. Monochrome and color available.
• Frame Rate: Shutters up to 100 frames per second.
• Fluidics: Micro-syringe pump with multiple sizes to optimize flow rates: 0.5 mL, 1 mL, 5 mL

Description:

Based on proven FlowCam DIPA technology, and optimized for larger particles (50μm to 5mm), FlowCam Macro provides rapid particle characterization that goes beyond just particle size. Direct, image-based measurements of particle size and shape enable differentiation of particle types in a heterogeneous mixture.

Specifications:

• Particle Count: Yes
• Concentration: Yes
• Particle Size (ESD): Yes
• Particle Size Range: 50μm to 5mm
• Technology: Dynamic Imaging Particle Analysis
• Image Type: 8-bit Grayscale (Monochrome Camera) or 24-bit Color (Color Camera)
• Image Format: 8-bit (monochrome) TIFF or 24-bit (color) TIFF
• Fluidics: High capacity industrial peristaltic pump, 2mm (deep) or 5mm (deep) flow cell
• Flow Rate: Up to 1 liter/minute

Description:

Introducing the FlowCam Nano® by Fluid Imaging Technologies. This patented oil immersion, flow imaging technology complements our industry-leading imaging particle analysis system to provide you with the most comprehensive particle analysis research and development tool for parenteral drug analysis.

Specifications:

• Method: Oil immersion flow microscopy
• Size Range: 300 nm to 30 μm
• Minimum Sample Volume: 20 μl
• Magnification & Flow Cell: 40X magnification with 50 μm flowcell
• Numerical Aperture (NA): 1.4 NA
• Camera’s Field of View: 150 μm height x 200 μm wide
• Camera Frame Rate: Up to 120 frames per second
• Focus Method: Manual
• Flow Rate: 0.02 mL/minute
• Image Format/Type: TIFF/ 8-bit Grayscale

Description:

These cost-effective vertical laminar flow and horizontal laminar flow workstations feature the Clean-Ceil fan filter module, which utilizes a HEPA filter that is 99.99% efficient for all particles >=0.3µm. The workstations may be used as either benchtop or console units. They incorporate a modular design approach that allows the user to customize a workstation by choosing the optional accessories necessary to meet individual requirements. The workstations come complete with aluminum support frame, clear Plexiglas side panels, 40% efficient ASHRAE-rated prefilter, white plastic laminate back panel (vertical model only, filter is on top), or clear Plexiglas top panel (horizontal model only, filter is on back).

Specifications:

• Dimensions (D x H x W): 21” x 22” x 46”
• Electrical: 115 V, 60 Hz
• Air velocity: 90 ±20 fpm

Features:

The Countess™ automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.

Specifications:

• User-friendly, benchtop design for simple, fast, automated cell count and viability measurements within a minute  
• Provides data on cell size and is compatible with a wide variety of eukaryotic cells without the need for any special changes between large or small sizes  
• Measures cell concentrations ranging from 1 × 104 to 1 × 107 cells/mL and cells with sizes ranging from 5 μm to 60 μm 
• Uses disposable counting chamber slides that eliminate washing steps and cross contamination between samples  
• Saves and prints cell count data including images using the Countess™ software and Countess™ USB drive  
• Presents comprehensive data with graphical reports and as a .CSV (comma separated value) file for sample comparisons  
• Allows you to gate and analyze cells based on cell size 

Specifications:

• Capacity: One 2-chamber disposable or 2-chamber reusable slide
• Cell Size: 4-60 µm (detection), 7-60 µm (viability)
• Concentration: 1x10^4-1x10^7 cells⁄mL
• Detection Method: Brightfield & 2 user-definable FL channels
• Dimensions: 9 in (228.6 mm) W x 5.5 in (139.7 mm) D x 9 in (228.6 mm) W
• Display: 7in Capacitive touch screen
• Field: 2.15 mm x 1.62 mm (3.48 mm2)
• Voltage: 100-240 VAC
• Weight (English): 8 lbs
• Memory: USB Stick

The BD FACSAria III sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary ease-of-use that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. The fluidics and optical systems are precisely integrated to maximize signal detection. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allows the system to achieve unrivaled sensitivity and resolution.

Specifications:

• Laser Configuration: 405 nm (violet), 488 nm (blue/green), 633 nm (red) lasers
• Manufacture Date: August 2010
• Catalog No: 648282-03

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.