Used Lab Equipment

Features:

The FastPrep® FP120 is a high-speed, benchtop reciprocating device for efficient
disruption of cell membranes. The unit is designed for use with the FastPrep®
Extraction Kits (Qbiogene, www.qbiogene.com). These kits are available for a
wide variety of extraction and purification applications.

Specifications:

• Controls: Programmable run time and speed; large LED readout
• Time: Range: 1-45 sec/ Programmable in 1 sec increments
• Speed: Range: 4.0-6.5m/sec/ Programmable in 0.5 m/sec increments
• Acceleration: < 2 seconds to maximum speed
• Deceleration: < 2 seconds to maximum speed
• Dimensions (W*D*H): 11 in x 15.75 in x 12 in

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

Includes:

• Computer w/ Software
• Monitor
• Manuals

Product Features:

• Automation of the standard trypan blue assay
• % Viability
• Total cell concentration
• Total viable cell concentration
• Mean cell size
• Real time cellular images
• Bioprocess monitoring
• Validated reagents
• Convenient reagent pack

 The Vi-CELL XR offers additional benefits over the S and AS Models:

• Extended size range to 2 microns, allowing additional analyses of yeast, and smaller cells.
• Increased sample throughput with greater efficiency.
• Reduced sample volume results in less reagent use.
• Auto focus capability increases instrument ease of use.
• Variable aspiration and mixing cycles optimize results.
• Enhanced image magnification provides unparalleled cellular detail.
• Circularity measurement helps isolate debris from cells.

 Specifications:

• Analysis Time: < 2.5 min
• Capacity: Single or 12 position via autoloader
• Concentration Range: 5 x 10^4 to 1 x 10^7 cells/mL
• Counting Accuracy: ± 6%
• Depth: 41 cm (16 in)
• Digitizing Resolution: 1394 x 1040
• Height: 44.5 cm (17.5 in)
• Imaging Technology: Auto-focus CCD array, Firewire camera
• Instrument Type: Video image through a quartz flow cell
• Overall Analysis Range: Size Range: 2 µm - 70 µm
• Sample Volume: 0.5 mL
• Temperature: 10°C to 40°C (50°F to 104°F)
• Viability Range: 0 to 100%
• Weight: 11.3 kg (25 lb)
• Width: 38 cm (15 in)

Description:

These cost-effective vertical laminar flow and horizontal laminar flow workstations feature the Clean-Ceil fan filter module, which utilizes a HEPA filter that is 99.99% efficient for all particles >=0.3µm. The workstations may be used as either benchtop or console units. They incorporate a modular design approach that allows the user to customize a workstation by choosing the optional accessories necessary to meet individual requirements. The workstations come complete with aluminum support frame, clear Plexiglas side panels, 40% efficient ASHRAE-rated prefilter, white plastic laminate back panel (vertical model only, filter is on top), or clear Plexiglas top panel (horizontal model only, filter is on back).

Specifications:

• Dimensions (D x H x W): 21” x 22” x 46”
• Electrical: 115 V, 60 Hz
• Air velocity: 90 ±20 fpm

Features:

The Countess™ automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.

Specifications:

• User-friendly, benchtop design for simple, fast, automated cell count and viability measurements within a minute  
• Provides data on cell size and is compatible with a wide variety of eukaryotic cells without the need for any special changes between large or small sizes  
• Measures cell concentrations ranging from 1 × 104 to 1 × 107 cells/mL and cells with sizes ranging from 5 μm to 60 μm 
• Uses disposable counting chamber slides that eliminate washing steps and cross contamination between samples  
• Saves and prints cell count data including images using the Countess™ software and Countess™ USB drive  
• Presents comprehensive data with graphical reports and as a .CSV (comma separated value) file for sample comparisons  
• Allows you to gate and analyze cells based on cell size 

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Features:

The Muse Cell Analyzer delivers accurate assessments of cell health parameters, cell signaling activation pathways & immune status in just minutes, revolutionizing the way you analyze cells.

Specifications:

• Cell Number per Assay: User defined; cell concentrations of 10,000–500,000 cells/mL
• Sample Format: Single loader
• Sample Volume: 200 µL
• Emission Max: Yellow: 28nm bandwidth at 576nm, Red: 30nm bandwidth at 680nm, Forward scatter = laser wavelength
• Excitation Max: Green Laser (532nm)
• Scatter: Forward
• Throughput: Less than 2 minutes per sample
• Dimensions 8.69” H  x 8.12” W x 11.11” L

Specifications:

• Loading time: App. 60 ul
• Measurement volume: 1.4 ul
• Analysis time: 50 sec (one step), 120 sec (two step)
• Dimensions: 38 x 26 x 22 cm (W x H x D)
• Weight: 6.7 lb (3 kg)

Features and Benefits

• Transfers take place in as little as 15–60 min
• Minimal buffer requirements save money
• Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
• Single-step locking system allows simple, speedy setup
• Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
• Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
• Safety cover breaks the electrical current when lifted, preventing electrical shock

Applications and Uses

• Western blotting
• Nucleic acid transfer
• Northern and Southern blot transfers in 10–35 min
• Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

Features:

• Maximum gel size (W x L), cm: 24 x 16
• Buffer requirement, ml: 200
• Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
• Recommended power supply: PowerPac HC (High Current)
• Dimensions (W x L x H), cm: 37 x 24 x 11
• Weight, kg/lb: 3.6/7.9

The Trans-Blot Turbo transfer system reduces western blotting setup time to 1 minute and transfer to as little as 3 without sacrificing sensitivity.

Paired with Trans-Blot Turbo transfer packs or Trans-Blot RTA kits, this system provides a complete solution for fast, high-efficiency transfer of even high molecular weight proteins.
Used on its own or as part of the V3 Western Workflow, the Trans-Blot Turbo system is the no-compromise solution for high-performance western blotting.

Features:

• Fast Transfer Times: Transfer your proteins in as little as 3 minutes.
• High Throughput: Transfer up to 4 mini or 2 midi gels simultaneously.
• Flexible Design: Easily customize transfer conditions; compatible with traditional semi-dry consumables.
• Ease of Use: Reduce set-up time to only 1 minute from opening the gel cassette to starting the transfer.
• Two Blotting Sandwich Options: Trans-Blot Turbo Transfer Packs or Trans-Blot Ready-to-Assemble Kits

Features:

Frontier 5000 Multi Pro Centrifuges are designed for universal use in research, industrial & clinical laboratories, and can be combined with our rotors & accessories for customized use in specific applications. Available in 5 models with capacities from low microliter to 6x250 ml, and featuring high-speed performance at maximum volume, automatic rotor identification and safety features - these multi-purpose centrifuges are a must for any lab.

Applications

Centrifugal Separation, General Sample Preparation, Cell Pelleting, Protein Separation, Nucleic Acid Separation, Cell Concentrating, Chemical Separation, Diagnostics, Environmental Sample Preparation, Blood Sample Processing

Display

Backlit Liquid Crystal Display (LCD)

Operation

Designed to be used with a variety of rotors and accessories

Communication

N/A

Construction

Corrosion-resistant stainless steel chamber with Polycarbonate/ABS plastic lid

Design Features

Ten acceleration & deceleration rates, selection of running time from 10s to 99h 59min or continuous, quick spin, CFC-free refrigeration system, sample temp. indication (refrigerated models), lid-locking system, over-speed protection, dual imbalance sensing

Specifications:

• Speed Range:200 rpm – 15,000 rpm

• Maximum Relative Centrifuge Force (x g):21,379 g

• Maximum Capacity (Rotor):6x250 ml

• Acceleration Gear:10

• Deceleration Gear:10

• Dimensions (HxLxW):13.94 in x 21.18 in x 17.56 in (354 mm x 538 mm x 446 mm)

• Display: LCD with backlight

• Net Weight：115 lb (52 kg)

• Noise Level:≤61±2 dB(A)

• Power Consumption: 520 W

• Rotor Recognition Method: Auto

• Running Time: Quickspin;  10 sec to 99 h 59 min 59 sec;  Continous

• Safety Certification: CE;  FCC

• Working Environment:36°F – 95°F, 80%RH, non-condensing (2°C – 35°C, 80%RH, non-condensing)

Features:

OHAUS Microplate Vortex Mixers are perfect for mixing microplates throughout the speed range. All models are designed for continuous-duty use in the lab. Digital models offer a timer and exact speed control for applications that demand repeatable results and non-digital variable speed models are an economical alternative. The high-speed and small orbit of these mixers make them optimal for effectively mixing microplates.

Applications

ELISA Assays, PCR Preparations, Digestions, Cell Disruptions

Display

Independent LED displays for speed/time allow the operator to view both settings simultaneously (digital models) and adjustment knob with dial markings for variable speed control (analog models)

Operation

Detachable 3-wire cord and plug (included)

Communication

N/A

Construction

Cycoloy

Design Features

Microplate adapter and cup head (included)

Specifications:

• Control：Analog

• Duty Rating：Continuous

• Orbit：0.1 in (3.5 mm)

• Speed Range：On Mode, 300 rpm – 2500 rpm;  Touch Mode, 300 rpm – 3500 rpm

• Capacity：2 Microplates

• Dimensions (HxLxW)：4.5 in x 10.5 in x 5.4 in (11.4 cm x 26.7 cm x 13.7 cm)

• Net Weight：11.7 lb (5.3 kg)

• Power：120V, 0.25A, 50/60Hz

• Power Consumption：30 W

• Safety Certification：TUV

• Working Environment:39°F – 104°F, 20% – 85%RH, non-condensing (4°C – 40°C, 20% – 85%RH, non-condensing)

 The Agilent 1100 HPLC G1321A Fluorescence Detector brings high-sensitivity and selection through simultaneous multi-wavelength detection. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. The FD's spectral information can be used for rapid method optimization and verification of separation quality.

Specifications:

• Dimensions: 5 x 13.5 x 17 in
• Weight:5 lbs
• Environment:0 to 40°C at <95% humidity
• Performance:10 fg Anthracene, Ex=250 nm, Em=400 nm*
• Performance:RAMAN (H2O) > 200 (FLF rev A)
• Performance:RAMAN (H2O) > 400 (FLF rev >A)
• Performance:Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds response time)
• Pulse Frequency:296Hz (single signal mode), 74Hz (spectral mode)
• Excitation Monochromator Range:200 nm - 700 nm
• Excitation Monochromator Bandwidth:20 nm
• Emission Monochromator Range:280 nm - 900 nm
• Emission Monochromator Bandwidth:20 nm
• Spectrum Acquisition Scan Speed:28 ms/datapoint
• Wavelength Characteristic Repeatability:+/- 0.2 nm
• Wavelength Characteristic Accuracy: +/- 3 nm
• Flow cells: 8 uL (standard) and 20 bar (2MPa) pressure maximum

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

• Simplified optical design for optimized baseline stability.
• Long-life Xenon lamp for highest sensitivity.
• Variable-mode monochromators for selective multisignal detection and online spectra acquisition.
• Easy front access to flow cell for fast inspection or exchange.

Specifications

• Detection type:  Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis
• Performance:  10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H20) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
• Light source: Xenon flash lamp, normal mode (20W) and economy mode (5W)
• Pulse frequency: 296 Hz for signal mode, 74 Hz for economy mode
• Excitation monochromator: Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm
• Emission monochromator: Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm
• Reference system: Inline excitation measurement
• Time programing: Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters
• Spectral acquisition: Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step
• Step size: 1—20 nm
• Spectra storage: Limited only by disk space
• Wavelength repeatability: ±0.2 nm
• Wavelength accuracy: ±3 nm
• Flow cells: Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz
• Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, 2 outputs
• Communications: Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.