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Bio-Rad S3e Cell Sorter (2-Lasers) 480nm / 640nm Cell Sorter

Bio-Rad S3e Cell Sorter (2-Lasers) 480nm / 640nm Cell Sorter

Cambridge ID #: 22262

This compact system is equipped with one laser, two fluorescence detectors and forward and side-scatter detectors.

Features of the S3e 488 Cell Sorter (one-laser):

  • Hands-free nozzle tip alignment features the fully automatic AutoGimbal System, driven completely by software
  • Simplified instrument setup fully automated drop delay calculation, stream alignment, and droplet break-off monitoring enable precise 14 color sorting with minimal training
  • 2-way cell sorting sort two different defined populations at the same time
  • High speed and high purity sort cells fast while maintaining high sensitivity and purity
  • Intuitive interface user-friendly ProSort Software enables effortless instrument control and sort logic definition

Manufacture Specification

  • Droplet frequency: 3743 kHz
  • Sorting type: True jet-in-air for high-performance sorting
  • Sorting rate: No hardware limitations for sort rate, limited only by droplet frequency and application
  • Sorting purity: >99% pure
  • Nozzle size: 100 m
  • Sorting direction: 2-way sorting
  • Sorting collection: Up to 5 x 5 ml sample tubes each direction
  • Up to 5 x 1.5 ml tubes each direction
  • Microscope slides
  • 8-well strip each direction
  • Lasers: Single-laser: 488 nm 100 mW
  • Single-laser upgrade: 488 nm 100 mW plus 561 or 640 nm 100 mW
  • Dual-laser: 488 and 561 or 640 nm 100 mW
  • Tri-laser: 405, 488, and 561 or 405, 488, and 640 or 488, 561, and 640 mW 100 mW
  • Detection: Forward scatter (FSC) with PMT
  • Side scatter (SSC) with PMT
  • Up to 4 fluorescence detectors with PMT: Minimum resolution: 0.5 m
  • Sensitivity: <125 MESF for FITC and PE
  • Temperature range: Sample and collection temperature control system: 437C
  • Peltier solid state system
  • Fluidics: Onboard fluidics and dilution of 8x sheath fluid with deionized water
  • Data format: FCS 3.1

 

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BD Biosciences FacsAria IIu Cell Sorter

BD Biosciences FacsAria IIu Cell Sorter

Cambridge Scientific ID #'s: 14996, 17981

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

 

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

 

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

 

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

 

Includes:

  • 3 lasers
  • Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
  • Computer w/ FACSDiva 8 Software
  • ACDU
  • 2 monitors
  • Fluidics Cart
  • Temperature Control
  • Lauda Chiller

 

Performance

Fluorescence Sensitivity

  • Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
  • FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
  • PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

  • Coefficient of variation (CV)
  • PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
  • Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

  • Doublet/singlet ratio:
  • CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

  • Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

  • Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

  • Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.
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BD Biosciences FacsAria IIu  - (4 Laser) Cell Sorter

BD Biosciences FacsAria IIu - (4 Laser) Cell Sorter

Cambridge ID #: 19571

Includes PM From BD and Guaranteed Installable under BD Service Contract

 

New Flow Cell installed in September 2018

Also included are the excitation laser lines, laser powers and optical configuration.

 

The new BD FACSAria IIu cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

 

The BD FACSAria IIu cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

 

The BD FACSAria IIu cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cellsorting.

 

Includes:

  • 4 lasers (375nm, 488nm, 405nm, 633nm)
  • Blue 7PMTs, Red 3PMTs, Violet 3PMTs
  • HP Computer w/ FACSDiva 8.0 Software
  • ACDU
  • 1 HP monitor
  • Fluidics Cart
  • Temperature Control
  • Lauda Chiller
  • Buffalo Whisper Aerosol Control
  • Under BD service contract since installed in 2012

 

Performance

Fluorescence Sensitivity

  • Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
  • FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
  • PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

  • Coefficient of variation (CV)
  • PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
  • Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

  • Doublet/singlet ratio:
  • CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

  • Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

  • Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

  • Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.
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Bio-Rad Trans-Blot SD Cell Semi-Dry Transfer Cell

Bio-Rad Trans-Blot SD Cell Semi-Dry Transfer Cell

Cambridge ID #: 22074

Features and Benefits

 

  • Transfers take place in as little as 15–60 min
  • Minimal buffer requirements save money
  • Capacity to transfer up to four Mini-PROTEAN precast or handcast gels, or three Criterion gels
  • Single-step locking system allows simple, speedy setup
  • Platinum-coated titanium anode plate electrodes and stainless-steel cathode plate electrodes provide consistent and reliable transfers, durability, and long life
  • Extra plastic templates required by other semi-dry blotters to prevent electrode short circuits are unnecessary
  • Safety cover breaks the electrical current when lifted, preventing electrical shock

 

Applications and Uses

 

  • Western blotting
  • Nucleic acid transfer
  • Northern and Southern blot transfers in 10–35 min
  • Transfer of DNA and RNA using the unique agarose gel semi-dry blotting support frame

 

Features:

 

  • Maximum gel size (W x L), cm: 24 x 16
  • Buffer requirement, ml: 200
  • Gel capacity: 4 Mini-PROTEAN precast or handcast gels, 3 Criterion gels, 1–3 PROTEAN II gel sandwiches
  • Recommended power supply: PowerPac HC (High Current)
  • Dimensions (W x L x H), cm: 37 x 24 x 11
  • Weight, kg/lb: 3.6/7.9
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Beckman Coulter Vi-Cell Auto Cell Viability Analyzer

Beckman Coulter Vi-Cell Auto Cell Viability Analyzer

Cambridge ID #: 14344

The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

Includes:

  • Computer w/ Software
  • Monitor
  • Manuals

Product Features:

  • Automation of the standard trypan blue assay
  • % Viability
  • Total cell concentration
  • Total viable cell concentration
  • Mean cell size
  • Real time cellular images
  • Bioprocess monitoring
  • Validated reagents
  • Convenient reagent pack

 Specifications:

  • Analysis Time: < 2.5 min
  • Capacity: Single or 12 position via autoloader
  • Concentration Range: 5 x 10^4 to 1 x 10^7 cells/mL
  • Counting Accuracy: ± 6%
  • Depth: 41 cm (16 in)
  • Digitizing Resolution: 1394 x 1040
  • Height: 44.5 cm (17.5 in)
  • Imaging Technology: Auto-focus CCD array, Firewire camera
  • Instrument Type: Video image through a quartz flow cell
  • Overall Analysis Range: Size Range: 2 µm - 70 µm
  • Sample Volume: 0.5 mL
  • Temperature: 10°C to 40°C (50°F to 104°F)
  • Viability Range: 0 to 100%
  • Weight: 11.3 kg (25 lb)
  • Width: 38 cm (15 in)
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Beckman Coulter Vi-Cell XR Cell Viability Analyzer

Beckman Coulter Vi-Cell XR Cell Viability Analyzer

Cambridge Scientific ID #'s: 14527, 26022, 20807

The new Vi-CELL Series Cell Viability Analyzers provide an automatic and cost effective means to perform the trypan blue dye exclusion method. This data is essential to the decision making process for basic tissue culture cell passage and maintaining optimum culture conditions in bioreactors. Cell and tissue culture applications in life science research, including recombinant protein and biopharmaceutical production, require the accuracy, precision and automation available only in the Vi-CELL cell viability analyzer.

The Vi-CELL automates the widely accepted trypan blue cell exclusion method. Historically, cell viability determinations were performed manually using a light microscope and hemacytometer. This technique has major shortcomings due to subjective determination of cell count, as well as manual, time consuming steps. In comparison to the manual technique, the Vi-CELL automatically performs the trypan blue with video imaging of the flow-through cell. Results are obtained in minutes.

Includes:

  • Computer w/ Software
  • Monitor
  • Manuals

Product Features:

  • Automation of the standard trypan blue assay
  • % Viability
  • Total cell concentration
  • Total viable cell concentration
  • Mean cell size
  • Real time cellular images
  • Bioprocess monitoring
  • Validated reagents
  • Convenient reagent pack

 The Vi-CELL XR offers additional benefits over the S and AS Models:

  • Extended size range to 2 microns, allowing additional analyses of yeast, and smaller cells.
  • Increased sample throughput with greater efficiency.
  • Reduced sample volume results in less reagent use.
  • Auto focus capability increases instrument ease of use.
  • Variable aspiration and mixing cycles optimize results.
  • Enhanced image magnification provides unparalleled cellular detail.
  • Circularity measurement helps isolate debris from cells.

 Specifications:

  • Analysis Time: < 2.5 min
  • Capacity: Single or 12 position via autoloader
  • Concentration Range: 5 x 10^4 to 1 x 10^7 cells/mL
  • Counting Accuracy: ± 6%
  • Depth: 41 cm (16 in)
  • Digitizing Resolution: 1394 x 1040
  • Height: 44.5 cm (17.5 in)
  • Imaging Technology: Auto-focus CCD array, Firewire camera
  • Instrument Type: Video image through a quartz flow cell
  • Overall Analysis Range: Size Range: 2 µm - 70 µm
  • Sample Volume: 0.5 mL
  • Temperature: 10°C to 40°C (50°F to 104°F)
  • Viability Range: 0 to 100%
  • Weight: 11.3 kg (25 lb)
  • Width: 38 cm (15 in)
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Invitrogen Countess III FL Automated Cell CounterImage Coming Soon

Invitrogen Countess III FL Automated Cell Counter

Cambridge Scientific ID #'s: 26354, 26351
This item is new to the Cambridge Scientific Used Lab Equipment Inventory. As a result, a product description is currently unavailable. For further information about this product, please inquire and a representative will contact you shortly.
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Beckman Coulter Vi-Cell MetaFlex Image Coming Soon

Beckman Coulter Vi-Cell MetaFlex

Cambridge ID #: 26020
This item is new to the Cambridge Scientific Used Lab Equipment Inventory. As a result, a product description is currently unavailable. For further information about this product, please inquire and a representative will contact you shortly.
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 C100 *NEW* Cell Counter

C100 *NEW* Cell Counter

Cambridge ID #: 25806

RWD automated cell counter is a reliable cell counting device with highly intelligent analysis software and excellent microscopy optics structure. It can realize accurate, safe cell-counting, and fluorescence analysis in 9 seconds, which liberates researchers from the heavy work of daily cell counting.

Specifications:

Optional Fluoresence application: ï¼ˆEx :375/28nm ï¼›Em:460/50nm),(Ex :480/30nm ï¼›Em:535/40nm),(Ex :540/25nm ï¼›Em:620/60nm)

Amplification Factor: 2.5×, 5.0 Megapixel
Focus Method: Manual focusing & Autofocusing
Counting Area: 2.15 mm×1.62 mm
Cell Type: Cell lines, stem cells, primary cells, pollens, beer yeast
Cell Size Range: 4-60 µm (Optimal: 7-60 µm)
Cell Concentration Range: 10^4-10^7 cells/mL
Cell counting Time: Less than 9s
Historical Data Storage: 1000 counting reports and images at most
Languages: Chinese and English
Dimensions: 212 mm(W)* 264 mm(H)* 165 mm (D)
Weight: ∼3.2 kg"

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 C100-Pro Cell Counter

C100-Pro Cell Counter

Cambridge ID #: 26030

RWD automated cell counter is a reliable cell counting device with highly intelligent analysis software and excellent microscopy optics structure. It can realize accurate, safe cell-counting, and fluorescence analysis in 9 seconds, which liberates researchers from the heavy work of daily cell counting.

Specifications:

Optional Fluoresence application: ï¼ˆEx :375/28nm ï¼›Em:460/50nm),(Ex :480/30nm ï¼›Em:535/40nm),(Ex :540/25nm ï¼›Em:620/60nm)

Amplification Factor: 2.5×, 5.0 Megapixel
Focus Method: Manual focusing & Autofocusing
Counting Area: 2.15 mm×1.62 mm
Cell Type: Cell lines, stem cells, primary cells, pollens, beer yeast
Cell Size Range: 4-60 µm (Optimal: 7-60 µm)
Cell Concentration Range: 10^4-10^7 cells/mL
Cell counting Time: Less than 9s
Historical Data Storage: 1000 counting reports and images at most
Languages: Chinese and English
Dimensions: 212 mm(W)* 264 mm(H)* 165 mm (D)
Weight: ∼3.2 kg"

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 C100-SE *NEW* Cell Counter

C100-SE *NEW* Cell Counter

Cambridge ID #: 25941

RWD automated cell counter is a reliable cell counting device with highly intelligent analysis software and excellent microscopy optics structure. It can realize accurate, safe cell-counting, and fluorescence analysis in 9 seconds, which liberates researchers from the heavy work of daily cell counting.

Specifications:

Optional Fluoresence application: N/A

Amplification Factor: 2.5×, 5.0 Megapixel
Focus Method: Manual focusing & Autofocusing
Counting Area: 2.15 mm×1.62 mm
Cell Type: Cell lines, stem cells, primary cells, pollens, beer yeast
Cell Size Range: 4-60 µm (Optimal: 7-60 µm)
Cell Concentration Range: 10^4-10^7 cells/mL
Cell counting Time: Less than 9s
Historical Data Storage: 1000 counting reports and images at most
Languages: Chinese and English
Dimensions: 212 mm(W)* 264 mm(H)* 165 mm (D)
Weight: ∼3.0 kg

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Invitrogen Countess  Automated Cell Counter

Invitrogen Countess Automated Cell Counter

Cambridge Scientific ID #'s: 25687, 22012

Features:

 

The Countess™ automated cell counter is a benchtop automated cell counter that performs cell count and viability measurements using trypan blue stain.

 

Specifications:

 

  • User-friendly, benchtop design for simple, fast, automated cell count and viability measurements within a minute  
  • Provides data on cell size and is compatible with a wide variety of eukaryotic cells without the need for any special changes between large or small sizes  
  • Measures cell concentrations ranging from 1 × 104 to 1 × 107 cells/mL and cells with sizes ranging from 5 μm to 60 μm 
  • Uses disposable counting chamber slides that eliminate washing steps and cross contamination between samples  
  • Saves and prints cell count data including images using the Countess™ software and Countess™ USB drive  
  • Presents comprehensive data with graphical reports and as a .CSV (comma separated value) file for sample comparisons  
  • Allows you to gate and analyze cells based on cell size 
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Invitrogen Countess II FL Automated Cell Counter

Invitrogen Countess II FL Automated Cell Counter

Cambridge ID #: 26200

Specifications:

  • Capacity: One 2-chamber disposable or 2-chamber reusable slide
  • Cell Size: 4-60 µm (detection), 7-60 µm (viability)
  • Concentration: 1x10^4-1x10^7 cells⁄mL
  • Detection Method: Brightfield & 2 user-definable FL channels
  • Dimensions: 9 in (228.6 mm) W x 5.5 in (139.7 mm) D x 9 in (228.6 mm) W
  • Display: 7in Capacitive touch screen
  • Field: 2.15 mm x 1.62 mm (3.48 mm2)
  • Voltage: 100-240 VAC
  • Weight (English): 8 lbs
  • Memory: USB Stick
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 Curiosis Celloger Nano *New* Automated Live Cell Imaging System

Curiosis Celloger Nano *New* Automated Live Cell Imaging System

Cambridge ID #: 24632

The NEW super compact and intuitive live cell imaging system, Celloger Nano – got everything you need to perform your sophisticate laboratory works. Equipped with exceptional fluorescence and auto-focusing technology, precise stage controller, and user-friendly software, accelerates your cell-based research works. Expand your cell-based research through our multifunction live cell imaging system, Celloger Nano.

Description:

  • One color fluorescence (GFP or RFP) and brightfield imaging technology
  • Compact size that easily fits into standard CO2 incubators
  • High quality system yet cost-effective
  • Compatible with various cell and tissue culture vessel types
  • Intuitive UI/UX and easy to acquire confluency data
  • Increased focus speed and reproducibility with reliable autofocusing function
  • Movie maker provides one-click easy way to make a time-lapse video

 

Specifications:
  • Dimensions:  211*146*188mm
  • Weight: 3.2kg (2.7kg / No Stage)
  • Objective Lens: 2X / 4X / 10X
  • Camera: 1.25MP / 5MP CMOS
  • Stage: Manual xy stage
  • Operating environment: 5-40℃, 20-95% humidity
  • File export format: TIFF, JPEG, PNG, AVI
  • Resolution: 1296 x 972 pixel (1.25MP) / 2592 x 1944 pixel (5MP)
  • Imaging positions: Single
  • Imaging mode: Bright-field / GFP / RFP
  • Vessel holders: Well plate(6-96), Petri dish(35-100), T-flask(25-75)
Application:
  • Wound healing assay
  • Cell migration
  • Cell morphology
  • Cell confluency
  • Cell proliferation
  • Cytotoxicity assay
  • Transfection efficiency assessment
  • Coculture monitoring
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 Curiosis Celloger Mini *New* Automated Live Cell Imaging System

Curiosis Celloger Mini *New* Automated Live Cell Imaging System

Cambridge ID #: 24630
Celloger Mini is an automated live cell imaging system based on bright-field microscopy with fully motorized stages.
This all-around and compact system provides autofocusing, time lapse imaging, and analytical software that lets you perform various types of sophisticated researches. Celloger Mini is designed to withstand the temperature and humidity suitable for the growth of cells that makes it compatible with CO2 incubators. Diversify your research paradigm with our integrated live cell imaging system, 
Celloger Mini.
 

Description:

  • Live cell monitoring and time-lapse imaging based on bright-field microscopy
  • Compact size that easily fits into standard CO2 incubators
  • Fully motorized stages and multi-position imaging up to 96 well plates
  • Compatible with various cell and tissue culture vessel types
  • Intuitive UI/UX and easy to acquire confluency data
  • Increased focus speed and reproducibility with reliable autofocusing function
  • Movie maker provides one-click easy way to make a time-lapse video
Specifications:
  • Dimensions:  195*305*220mm
  • Weight: 4.5kg
  • Objective Lens: 4X
  • Camera: 1.25MP / 5MP CMOS
  • Stage: Motorized XYZ stage
  • Operating environment: 5-40℃, 20-95% humidity
  • File export format: TIFF, JPEG, PNG, AVI
  • Resolution: 2592 x 1944 pixel (5MP)
  • Imaging positions: up to 1000
  • Imaging mode: Bright-field 
  • Vessel holders: T-flask A25, Petri dish 35mm / Petri dish 60mm
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