Used Lab Equipment

Features:

• BD CSampler- 96 well plate and 24 tube rack automated sampler for BD Accuri C6 flow cytometer  
• Blue and red laser
• Two light scatter detectors
• Optical filters such as FITC, PE, PerCP and APC
• A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use
• Optional filters and the Selectable Laser Module expand the available fluorochrome combinations
• Compact system and portable weight (13.6 Kg)
• Intuitive interface of the software
• Measures: 11 x 14.75 x 16.5 inches (H x W x D) (27.9 x 37.5 x 41.9 cm)

• Configuration: B4-R2-V0 (i.e. 6 detectors, 2 lasers: 4 colors enabled off the blue laser, 2 off the red, the violet laser is not enabled.
• A USB drive can be purchased from manufacturer to enable additional fluorescent channels, including off of the violet laser, instead of buying an entire new instrument).
• Ref#: At the time of purchase, the ref# for this product was B53013. BC has changed their ref #s and these specs now correspond to ref # C09748.
• Date of manufacture: 6/2018.
• Purchased new from manufacturer on 8/2018.
• Condition: Like new. I.e., the instrument has never had true samples in it. It was only run once, as a demonstration -- the Beckman Coulter team used their test samples to show the instrument’s functionality. (Explanation: purchased by administrators and not aligned with needs of research staff, who did not use it).
• All original drives, accessories included.
• This includes license USB (required to authorize daily use), software USB (required to set up instrument for use), documentation, Packing slip, setup guide)
• Cords, sheath fluid containers, etc. Original reagents have since expired.

Single Laser Configuration

Includes Computer Loaded with Cytosoft Software

The guava easyCyte flow cytometry systems are cost-effective, easy to use and deliver complete and comprehensive cell analysis- right on your benchtop. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers—all while providing the analytical power you need to expand your research horizons.

Features:

• Blue Laser
• Forward and Side Scatter
• Tube Sampling
• Automated 96 Well Micrplate Sampling
• Small Sample Size (<20 μL)
• Minimal waste (<50 mL per day)
• Detectors
  • Green
  • Yellow
  • Red

Fluidics Specifications:

• Flow Cell Dimension: Standard square capillary with ID of 100 µm
• Pump: Positive displacement
• Sheath Fluid: None
• Sample Flow Rate: 7µL/min to 72 µL/min
• Waste Generation: typically <40mL in 8 hours of continuous use
• Dead Volume: 50 µL (for 96-well microplate)
• Sample Requirement: as few as 2,000 cells/test; typically 25,000 – 100,000 cells
• Cell Size: 1-60 µm
• Typical # of Cells/Protocol: 1,000 – 10,000 cells/test

Software and the computer are included. The machine has 3 lasers which can run 8 colors.3 lasers: red (633), blue (488) and violet (465). Runs 8 colors. Blue has 5 PMT with Sidescatter. red has 2 PMT. Violet has 2 PMT.

Specifications:

(BLUE)

Wavelength: 488 

Inteded Dyes for PMT Position (nm) A: PE-Cy7

Inteded Dyes for PMT Position (nm) B: PerCP, PerCP-Cy5.5

Inteded Dyes for PMT Position (nm) C: N/A

Inteded Dyes for PMT Position (nm) D: PE

Inteded Dyes for PMT Position (nm) E: FITC 

Inteded Dyes for PMT Position (nm) F:  Side Scatter (SSC)

(RED)

Wavelength: 633

Inteded Dyes for PMT Position (nm) A: APC-Cy7

Inteded Dyes for PMT Position (nm) B: N/A

Inteded Dyes for PMT Position (nm) C: APC

(VIOLET)

Wavelength: 405

Inteded Dyes for PMT Position (nm) A: AmCyan v500

Inteded Dyes for PMT Position (nm) B: Pacific Blue 

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software

• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• Laser
• 6 PMTs unit and side scatter

• All Necessary Cables
• Manuals

Optics Specifications:

• Lasers
  • Air-cooled:
  • 488-nm solid state, 20-mW laser output
  • 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 0 μm
• SSC Resolution: 5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP
• Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
• Blue Laser Dyes
  • FITC, PE, PerCP or PerCP-Cy™5.5,
  • PE-Cy7 (525, 575, 678 or 695, 785 nm)
• Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
• Detector Bands
  • Blue Laser: 530/30; 585/42; >670; 780/60 nm
  • Red Laser: 660/20; 780/60 nm
• Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software
• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• 3 PMTs in 4-2 configuration w/ side scatter
• All Necessary Cables
• Manuals

Optics

Lasers

• Air-cooled
• 488-nm solid state, 20-mW laser output
• 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 1.0 μm
• SSC Resolution: 0.5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP

• Fluorescence Detectors: 6 PMTs in 4-2 configuration w/ side scatter

Specifications:

 Lasers: 20-mw 488 Argon Ion 

• Detectors: FS, SS, 5 FL PMT 
• Flow Cell: 150x450 micron rectangular Channel 
• Sample Loading: 96-well, 24-well, 40-tube 
• Signal Processing: 20-bit Digital Signal Processing 
• Software: MXP v 2.2 
• Overall Analysis Range:5-40 micron particle size 
• Sample Volume: 25-500 microliters 
• Weight: 187 lbs 
• Width: 5 inches 

"The Millipore Guava EasyCyte HT offers sample handling formats for both tubes and 96-well plates, direct sampling using patented microcapillary system, and a user-friendly, versatile software. EasyCyte instruments are also compatible with many fluorochromes and fluorescent reagents.

Specifications:

Operating temperature: 16°–35°C (60°–95°F)

Operating humidity: 10–90% relative humidity (non-condensing)

Power: 100–240 VAC, 50/60 Hz, 120W

Dimensions: 21 x 24 x 12 inches

Weight: 71lb

Detection: 5 colors including forward scatter

Laser: blue 488 nm (50mW)

Flow cell dimension: standard square capillary with ID of 100 µm

Pump: 7 µL/min to 36 µL/min

Sample flow rate: positive displacement

Waste and cleaning vials: 30-mL glass vial with screw top

Waste generation: typically <40 mL in 8 hours of continuous use

Dead volume: 50 µL (for 96-well microplate); 75 µL (for 0.5-mL microcentrifuge tubes)

Sample concentration: final particle concentration of 104 to 5 x 105 particles/ml

Sample requirement: for accurate results (up to 2.5 x 106 beads/mL for the RapidQuant Assay) as few as 2,000 cells/test; typically 25,000-100,000 cells/test, depending on the assay

Data management: Output data file formats - (1) binary data storage in FCS 3.0 format; (2) spreadsheet results file in CSV format"

The Tali Image-Based Cytometer is a 3-channel (bright field, green fluorescence, red fluorescence) benchtop assay platform that uses state-of-the-art optics and image analysis to perform assays for cells in suspension, including GFP and RFP expression, apoptosis, cell viability (live, dead, and total cells), cell cycle, and cell counting assays. It is compatible with a wide variety of eukaryotic cells. In addition to the bright field channel, the Tali® Image-Based Cytometer features two fluorescent channels (green and red), enabling it to simultaneously count green or red fluorescent stains, as well as cells expressing GFP and RFP.

Specification:

• Instrument type: Benchtop cell counter and suspension cell-based assay platform
• Dimensions (W x D x H): 11.5 x 17.5 x 11.5 in.
• Weight: 19.4 lbs
• Operating power: 100–240 VAC, 2.5 A, 120 V
• Frequency: 50/60 Hz
• Electrical input: 12 VDC, 13 A
• Installation site: Indoor use only, Class A environments; pollution degree 2
• Operating temperature: 10 - 40°C
• Operating humidity: < 80% (non-condensing)
• Processing time: 10 seconds to 2 minutes, depending on the number of fields captured
• Sample concentration range: 1 x 10^5 - 1 x 10^7 cells / mL
• Particle/cell diameter range: 5-60 µm
• Required sample volume: 25 µL
• Firmware: Tali Image-Based Cytometer Firmware
• USB drive: 4 Gigabyte
• Optics: 3 channels (bright field, green fluorescence, red fluorescence)
• Excitation: Green channel LED (458 ± 20 nm), Red channel LED (530 ± 20 nm)
• Green channel filter: 466/40 EX, 495 LP Di, 525/50 EM
• Red channel filter: 543/22 EX, 580 LP Di, 585 LP EM
• Camera: 1.3 Mega pixels, 4x objective, 4x or 16x digital zoom

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Includes PM From BD and Guaranteed Installable under BD Service Contract

New Flow Cell installed in September 2018

Also included are the excitation laser lines, laser powers and optical configuration.

The new BD FACSAria IIu cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria IIu cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria IIu cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cellsorting.

Includes:

• 4 lasers (375nm, 488nm, 405nm, 633nm)
• Blue 7PMTs, Red 3PMTs, Violet 3PMTs
• HP Computer w/ FACSDiva 8.0 Software
• ACDU
• 1 HP monitor
• Fluidics Cart
• Temperature Control
• Lauda Chiller
• Buffalo Whisper Aerosol Control
• Under BD service contract since installed in 2012

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.