Used Lab Equipment

Features:

Mini 48 DNA purification instrument is used to automatically purify plasmid DNA directly from bacterial culture in just over one hour.

Specifications:

• Electrical: 110V

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Features:

• Simple column selection with huge built-in library
• Automatic titration of buffers across a broad pH range
• Quick and easy transfer of methods to production-scale
• UNICORN method wizard High performance purification and characterization
• State-of-the-art computer-controlled pumps, UV/conductance monitors, and fraction collector
• UV-Vis detection of up to three wavelengths simultaneous

Specifications:

• Flow rate: 0.01-100 ml/min
• Operating pressure max.: 10 MPa
• Tubing i.d. (flow path): 0.75 mm
• UV wavelength: 190-700 nm
• Flow cell path length: 2 mm
• pH monitoring: 2–12
• Buffer selection: 11 buffer inlets
• Relative humidity: 10%-95% (non condensing)
• pH Stability: 1-14
• Viscosity: max 5 cP
• Multiple wavelength detection: Yes, up to 3 wavelengths
• Multiple sample injection: Yes, up to 8 samples
• Autosampler injection: Optional
• Automatic buffer preparation: BufferPrep
• Pump type: Piston pump, metering type
• Application: Purification of microgram to gram quantities of protein
• Dimensions (W x H x D): 480 x 610 x 470 mm
• Power Requirements: 100-120/220-240 VAC, 600W

Description:

Purifier Vertical Clean Benches direct HEPA-filtered air downward over the work area to provide ISO Class 5 (formerly Class 100) conditions and protect your work from particulate contamination. Applications for clean benches include plant tissue culture, media plate preparation, electronics inspection, medical device assembly and pharmacy drug preparation. Because they do not provide protection to the user, they should not be used in conjunction with biohazardous material, toxins or radionuclides.

Specifications:

• Weight: 125.0 lbs (57.0 kg)
• Dimensions (W x D x H): 24" x 28.6" x 36.8"
• Electrical: 115 volts, 60 Hz, 5 A
• Laminar Flow: Vertical
• Nominal Width: 2'
• Conformance: CAN/CSA C22.2, UL, UL Standard 3101-1/61010-1
• Nominal downflow velocity of 45-65 fpm
• Aerodynamic air foil
• Supply HEPA filter, 99.99% efficient on particles 0.3 micron
• ISO Class 5 air (formerly Class 100 per Federal Standard 209E)
• Variable speed built-in blower with motorized impeller and solid state control rated for 10 amps
• Intrinsically-sound, negative pressure design
• Minihelic pressure gauge
• Air diffuser
• UV-resistant, glacier white and gray, powder-coated aluminum and steel construction
• UV-opaque and resistant 1/4" thick tempered safety glass sash and sides
• Fluorescent lamp
• Light and blower switches
• 9.4" high sash opening
• Ergonomic 10° angled and hinged sash that pivots upward, locks to a loading height of 20" and has wiping seals to promote ISO Class 5 conditions

Includes:

• Applied Biosystems 3730XL
• 96-Capillary
• Computer w/ Pre-loaded existing software
• Data Collections 3.0
• Warranty Period: 90 days
• Installation on site

Specifications 

• Laser: Argon-ion multi line, single mode laser: primary excitation lines 488 and 515.5nm
• Oven Temp: 18°C to 70°C
• Sample Volumes: 384 well plates = 5-30µl / 96 well plates = 10-50µl
• Plate Seal: Septa or Polypropylene heat seal
• Operating Environment: 18°C - 30°C, Humidity = 20 to 80%
• Capillary Array Lengths: 36 cm or 50 cm
• Capillary Dimensions: 150 5m o.d. / 50 5m i.d.
• Polymer Consumption
  • 36cm: approx. 200µl per run
  • 50cm: approx. 250µl per run

ABI 3730xl DNA Sequencer         

• Optics
  • In-capillary detection by dual-side illumination.
  • Multiple fiter sets: Z, E, G5, G5-RCT, Any4Dyeset, Any5Dyeset and S (optional)
  • Backside thinned CCD for maximum optical sensitivity
• Capillaries
  • Choice of capillary lengths, 36cm or 50cm
  • Dimensions: 150µm o.d./50µm i.d.
• Reagent Handling
  • Automated polymer replenishment pump for increase throughput
  • POP-7 performance robust polymer separation matrix
  • 5 sequencing run modules, 1 fragment analysis run and SNPlex run module
  • On-board reagents for up to 48-hours of unattended operation
• Performance Per Run Type  
  • LongSeq: 1152 samples per day (980 Kbases)
  • StdSeq: 2304 samples per day (1550 Kbases)
  • FastSeq: 2304 samples per day (1600 Kbases)
  • RapidSeq: 3840 samples per day (2100 Kbases)
  • GeneMapper Software
    • 4224 samples per day (92,000 genotypes)
    • 36 cm, up to 500 bp resolution with 0.15 bp sizing precision in 34 minutes

Power Requirements       

• Voltage: 200-220 volt or 230-240 volt

System Dimensions        

• Width 100 cm
• Depth 73 cm
• Height 89 cm
• Weight 180 kg

Description:

The Perkin Elmer Cetus DNA Thermal Cycler allows for flexible temperature programming, step duration and rapid heating and cooling from step to step for consistent results. Features a 48-position aluminum block for 0.5ml GeneAmp Reaction Tubes.  Includes four pre-programmed files: Soak, Time Delay, Thermo-Cycle and Step-Cycle files and 2 demonstration files: Gene-Amp Thermo-cycle and Gene-Amp Step-cycle (93 programmable user files are available). File modifications include, Automatic Segment Extension, Infinite Post-PCR Subambient Incubation.

Specifications:

• Dimensions (H x W x D): 12" x 13" x 19.25"
• Power Req: 120 V, 50/60 Hz, 1000 W

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

The DNA Thermal Cycler 480 automates the polymerase chain reaction (PCR) technique for amplifying DNA.

Specifications:

• Capacity: 48 x 0.5 mL
• Temperature Range: -5°C to 100°C

This LCMS brings you the best in quantitative sensitivity and robustness to expand upon the experiments going beyond MRM, and give you substantial improvements in data quality.

Includes:

• AB Sciex 4500 LC/MS/MS
• Shimadzu Prominence 20 UFLC  
• Shimadzu DGU-20A3 Degasser
• (2)Shimadzu LC_20AD Liquid Chromatograph
• Shimadzu CTO-20AC Column Oven
• Shimadzu SIL-20AC UFLC
• Windows 7 Computer Loaded with Analyst 1.6 Software       

Specifications:

• Sensitivity MRM: mode Reserpine 200 fg on column S/N > 2000  C.V. < 5%
• Maximum scan speed: 12,000 Da/sec
• Polarity switching time: 50 msec, in MRM and scheduled MRM (sMRM) modes
• Minimum MRM dwell time: 1 msec
• Mass range (m/z): 5-2000
• Cross talk for reserpine 609/195: <0.5% cross talk detectable with 2 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion MS for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), and scheduled MRM (sMRM)
• Detector type: AcQuRate Pulse Counting CEM
• Dynamic range: 4 orders of magnitude
• Ionization sources: Turbo V source housing with TurboIonSpray probe or APCI probe.
• Maximum temperature: 750ºC
• Flow rate compatibility: 5 μL/minute to 3 mL/minute
• Power: 208V 50/60Hz AB Sciex 4500, 208V Vacuum Pump, 208V Peak N2 Generator, 115V Shimadzu UPLC
• Dimensions: 37" x 33" x 55" Sciex 4500
• Weight: 250lbs

 Includes One Year Service Contract, PM at 6 Months, and Installation

The AB SCIEX QTRAP 5500 System is a high sensitivity, bench top hybrid triple quadrupole-Linear Accelerator trap mass spectrometer designed for LC/MS/MS analyses. This instrument provides an uncompromised combination of Linear Accelerator trap and triple quadrupole functionality, uniquely allowing both  qualitative and quantitative analyses to be carried out in a single experiment.

Includes:

• AB Sciex 5500 Qtrap LC/MS/MS
• Agilent 1200 HPLC System
  
  • Agilent G1322A Degasser
  • Agilent G1311A Quat Pump
  • Agilent G1315D DAD (Diode Array Detector)
• Solvent Tray
• Computer Loaded with Analyst 1.6 Software       

Specifications

• Sensitivity MRM mode Reserpine: 50 fg on column S/N > 2000
• V.: < 5%
• Triple quad scan speed: 12,000 Da/sec
• Linear ion trap scan speed: 20,000 Da/sec
• Polarity switching time: 50 msec
• Minimum MRM Dwell Time: 1 msec
• Triple quad mass range (m/z): 5-1250
• Linear ion trap mass range (m/z): 50-1000
• Cross talk for Reserpine 609/195: No significant cross talk detectable with 1 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), enhanced MS scan, enhanced product ion scan, enhanced resolution scan, MS  scan, MRM3 scan, and TripleTrap scan modes

Includes

• Gene Pulser II
• Capacitance Extender Plus
• Pulse Controller Plus