Used Lab Equipment

Includes:

• Applied Biosystems 3730XL
• 96-Capillary
• Computer w/ Pre-loaded existing software
• Data Collections 3.0
• Warranty Period: 90 days
• Installation on site

Specifications 

• Laser: Argon-ion multi line, single mode laser: primary excitation lines 488 and 515.5nm
• Oven Temp: 18°C to 70°C
• Sample Volumes: 384 well plates = 5-30µl / 96 well plates = 10-50µl
• Plate Seal: Septa or Polypropylene heat seal
• Operating Environment: 18°C - 30°C, Humidity = 20 to 80%
• Capillary Array Lengths: 36 cm or 50 cm
• Capillary Dimensions: 150 5m o.d. / 50 5m i.d.
• Polymer Consumption
  • 36cm: approx. 200µl per run
  • 50cm: approx. 250µl per run

ABI 3730xl DNA Sequencer         

• Optics
  • In-capillary detection by dual-side illumination.
  • Multiple fiter sets: Z, E, G5, G5-RCT, Any4Dyeset, Any5Dyeset and S (optional)
  • Backside thinned CCD for maximum optical sensitivity
• Capillaries
  • Choice of capillary lengths, 36cm or 50cm
  • Dimensions: 150µm o.d./50µm i.d.
• Reagent Handling
  • Automated polymer replenishment pump for increase throughput
  • POP-7 performance robust polymer separation matrix
  • 5 sequencing run modules, 1 fragment analysis run and SNPlex run module
  • On-board reagents for up to 48-hours of unattended operation
• Performance Per Run Type  
  • LongSeq: 1152 samples per day (980 Kbases)
  • StdSeq: 2304 samples per day (1550 Kbases)
  • FastSeq: 2304 samples per day (1600 Kbases)
  • RapidSeq: 3840 samples per day (2100 Kbases)
  • GeneMapper Software
    • 4224 samples per day (92,000 genotypes)
    • 36 cm, up to 500 bp resolution with 0.15 bp sizing precision in 34 minutes

Power Requirements       

• Voltage: 200-220 volt or 230-240 volt

System Dimensions        

• Width 100 cm
• Depth 73 cm
• Height 89 cm
• Weight 180 kg

Description:

The HiSeq 2500 system is a high throughput sequencing system that combines scalable output with flexible (dual) run modes which supports multiple applications, and superior turnaround times. With rapid or high-output modes and single or dual flow cell options, the HiSeq 2500 can be tuned to meet output needs ranging from 9 Gb to 1 Tb or 300 million to 4 billion reads per run. This system is a 2014 model that has recently been deinstalled by Illumina and includes a cBot. It can run the V3 high output chemistry as-is, or V4 with a hardware upgrade from Illumina.  This can also run the most recent version of the rapid run chemistry.

Specifications:

• Dimensions - W x D x H: 118.6 cm x 76.0 cm x 94.0 cm (46.7 in x 30.0 in x 37.0 in)
• Weight: 221.4 kg (488 lbs)
• Dual mode processing (High Output and Rapid Run)
• HiSeq Control Software
• Instrument Control Computer                       
• Base Unit: Intel Xeon CPU E5-2620 @ 2.00 GHz (2 processors)
• Memory: 64 GB RAM
• Hard Drive: 8 x 1.0 TB 3.5” Enterprise SATA
• Operating System: Microsoft Windows 7
• Power Requirements: 100 - 240V AC 50/60Hz, 20A, 1500 W
• Instrument Configuration: Computer and touch screen display Installation setup and accessories Data collection and analysis software
• Laser: 532 nm, 660 nm, 650 nm

Description:

The Perkin Elmer Cetus DNA Thermal Cycler allows for flexible temperature programming, step duration and rapid heating and cooling from step to step for consistent results. Features a 48-position aluminum block for 0.5ml GeneAmp Reaction Tubes.  Includes four pre-programmed files: Soak, Time Delay, Thermo-Cycle and Step-Cycle files and 2 demonstration files: Gene-Amp Thermo-cycle and Gene-Amp Step-cycle (93 programmable user files are available). File modifications include, Automatic Segment Extension, Infinite Post-PCR Subambient Incubation.

Specifications:

• Dimensions (H x W x D): 12" x 13" x 19.25"
• Power Req: 120 V, 50/60 Hz, 1000 W

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

The DNA Thermal Cycler 480 automates the polymerase chain reaction (PCR) technique for amplifying DNA.

Specifications:

• Capacity: 48 x 0.5 mL
• Temperature Range: -5°C to 100°C

Features:

Mini 48 DNA purification instrument is used to automatically purify plasmid DNA directly from bacterial culture in just over one hour.

Specifications:

• Electrical: 110V

Includes:

• Agilent G2938A Bioanalyzer
• Computer with Bioanalyzer Software
• Vortexer
• Priming Station

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Results are delivered within 30-40 minutes in automated,

high quality digital data.

Miniaturization of analytical instrumentation has many advantages over conventional techniques. These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows

Areas of Application:

• On-chip flow cytometry
  • The easy way to acquire dual-color, cell-based fluorescence data
• RNA quality check with RIN
  • The industry standard for RNA analysis offering total RNA, mRNA and Small RNA's data including RIN algorithm (RNA Integrity Number)
• DNA size and quantity
  • Smart, high resolution separation and quantification of DNA Highly sensitive DNA sample QC for next generation sequencing experiments
• SDS-PAGE replacement for protein analysis
  • The fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity

Features:

• Faster than gels – analyze 10-12 samples in 30-40 minutes
• Easy to use – load the chip, press "start“, the instrument does the rest
• Ready-to-use assays and pre-packaged reagent kits
• Reproducible data, contamination-free hardware
• Quick and easy sample comparison – one-click overlay, scaling or zooming features
• Reproducible, complete and digital data
• Easy handling, storage and exchange of digital data
• Quantification of each individual fragment per sample offers purity results 2-color analysis of fluorescently stained cells
• Data is acquired and archived in digital format.

Includes:

• Agilent G2938C Bioanalyzer
• Computer with Bioanalyzer Software
• Vortexer
• Priming Station

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Results are delivered within 30-40 minutes in automated,

high quality digital data.

Miniaturization of analytical instrumentation has many advantages over conventional techniques. These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows

Areas of Application:

• On-chip flow cytometry
  • The easy way to acquire dual-color, cell-based fluorescence data
• RNA quality check with RIN
  • The industry standard for RNA analysis offering total RNA, mRNA and Small RNA's data including RIN algorithm (RNA Integrity Number)
• DNA size and quantity
  • Smart, high resolution separation and quantification of DNA Highly sensitive DNA sample QC for next generation sequencing experiments
• SDS-PAGE replacement for protein analysis
  • The fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity

Features:

• Faster than gels – analyze 10-12 samples in 30-40 minutes
• Easy to use – load the chip, press "start“, the instrument does the rest
• Ready-to-use assays and pre-packaged reagent kits
• Reproducible data, contamination-free hardware
• Quick and easy sample comparison – one-click overlay, scaling or zooming features
• Reproducible, complete and digital data
• Easy handling, storage and exchange of digital data
• Quantification of each individual fragment per sample offers purity results 2-color analysis of fluorescently stained cells
• Data is acquired and archived in digital format.

For use where gentle, low foaming agitation and uniform mixing of lab samples are required. Applications include immunoassays, hybridizations, mixing blood samples, coupling affinity chromatography media and DNA extractions. 

Specifications:

• Dimensions: 7 x 7.5 x 5 in
• Platform Dimensions: 12 x 12 x 12 in
• Weight: 5 lbs (3.4 kg)
• Speed: 6 – 23 orbits per minutes
• Tilt Angle: 0 ° - 23 °

Description:

The Bio-Rad Model 3000xi Computer Controlled Power Supply produces a constant voltage to 3,000 volts, constant current to 300 mA, and constant power to 400 watts. This fully switching, microprocessor controlled unit may be used with any electrophoresis instrument. The high outputs make the Model 3000xi Power Supply ideal for electrofocusing, DNA sequencing, and isotachophoresis. The supply is useful as a general purpose instrument, and is recommended for SDS-PAGE electrophoresis, two-dimensional electrophoresis, native gel electrophoresis, electrophoretic blotting, and DNA/RNA separations. The 3000xi Power Supply is a fully programmable and computerized instrument that incorporates several unique features. The supply offers four operating modes: standard, time, volt-hour, and step. The operator has a choice of running electrophoresis manually, for a set period of time, or for a set number of volt-hours. These parameters can be combined using the step mode. While operating in any one of the four modes, the user simply enters the desired power conditions and begins the run. The operation parameters are displayed on the LCD. Output voltage, current, and power are displayed on the LED display. Electrophoresis running conditions can also be programmed. Up to 10 routines can be saved in the Model 300xi Power Supply's memory, where they can be recalled at any time. This is especially useful when using the special step mode which allows three separate electrophoresis running conditions to be programmed simultaneously. A single gel can then be run safely at three different power conditions without the need for constant monitoring.

Features:

• Output Voltage: 25-3000 Volts DC
• Output Current: 0-300 mA
• Output Power: 0-400 W
• Overload and Short Protection: Circuit breaker
• Leakage Detection: Lamp and alarm >500 µA
• Operating Temperature: 0-40°C
• Dimensions (W x H x D): 40 x 32 x 11.5 cm
• Weight: 9.7 kg

Features:

Applied Biosystems 3130 (4-capillary) Genetic Analyzer was refurbished by the original manufacturer to meet both manufacturing and product specifications. Availability may be limited.

Applied Biosystems 3130 Genetic Analyzer is the latest generation of 4-capillary electrophoresis instruments for the low to medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing you to save time, reduce costs and increase productivity.

Specifications:

• Capillary Length: 22cm, 36cm, 50 cm, 80cm
• Current Limit: 15 A
• Dimensions: Depth: 55cm, Height: 81cm, Width (open): 149cm
• For Use With (Equipment): 3130 Genetic Analyzer
• Format: 384-well plate, 96-well plate
• Number of Capillaries: 4 Capillaries
• Operating Environment:Humidity: 20%-80%, Temperature: 15°C-30°C
• Operating System: Microsoft Windows 7
• Performance: 98.5% base calling accuracy
• Polymer Consumption: Up to 960 samples per 7000µl Bottle
• Product Size: 1 instrument
• Read Length: Up to 950bp
• Sample Volume: > 10µL
• Throughput: 30,400 bases/24hr (Long Read Sequencing), 4,320 genotypes/24hr (4-dye DNA Sizing), 5,760 genotypes/24hr (5-dye DNA sizing), 82,000 bases/24hr (Ultra Rapid Sequencing)
• Voltage Limit: 200-220V or 230-240V
• Weight: 130 kg

Features:

The FlashGel System for fast electrophoresis of DNA is the ideal sample screening tool. Check up to 32 PCR or restriction fragments quickly without having to plan your day around agarose gels or verify your DNA sample integrity prior to downstream analysis. The FlashGel System is the fastest way to separate DNA and the best way to watch DNA migration as it happens. This revolutionary new tool separates DNA in 2 - 7 minutes. Monitor gel runs right at the bench, without UV light. The highly sensitive system allows a 5-20X reduction in DNA levels - saving both time and money.

Specifications:

• 5 minute separation and recovery

• See bands in as little as 2 minutes

• Recover samples directly without UV light band excision or purification,

• Real-time separation and documentation

• Watch band migration as it happens

• Photograph gels at the bench without DNA damaging UV light

• Outstanding sensitivity and resolution

Features:

The FlashGel Camera captures data from the FlashGel System and say goodbye to darkrooms and UV light. Complete separation and documentation safely, at your bench, in minutes. This simple digital camera in an enclosed hood connects directly to your laptop or PC via USB cable.

Specifications:

• 5 minute separation and recovery, See bands in as little as 2 minutes

• Recover samples directly without UV light band excision or purification

• Real-time separation and documentation

• Watch band migration as it happens

• Photograph gels at the bench without DNA damaging UV light

• Outstanding sensitivity and resolution

Ideal for most applications including protein mini-gels, SDS-PAGE, and DNA electrophoresis.

Specifications:

• Temperature Range: 0 to 40 °C
• Dimensions: 10” D x 7.5” W x 4” H