Used Lab Equipment

Used Lab Equipment

We offer an assortment of used and refurbished Thermal Cyclers ranging from Real Time PCR to qPCR, and Gradient Thermal Cyclers to non-Gradient Thermal Cyclers. Some of our popular manufacturers include ABI, MJ-Research, Bio-Rad, Agilent / Stratagene, and Eppendorf.

Dna Sequencer: 1-15 of 53

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Applied Biosystems 3730XL DNA Sequencer

Applied Biosystems 3730XL DNA Sequencer

Cambridge ID #: 7164

Includes:

  • Applied Biosystems 3730XL
  • 96-Capillary
  • Computer w/ Pre-loaded existing software
  • Data Collections 3.0
  • Warranty Period: 90 days
  • Installation on site

 

Specifications 

  • Laser: Argon-ion multi line, single mode laser: primary excitation lines 488 and 515.5nm
  • Oven Temp: 18°C to 70°C
  • Sample Volumes: 384 well plates = 5-30µl / 96 well plates = 10-50µl
  • Plate Seal: Septa or Polypropylene heat seal
  • Operating Environment: 18°C - 30°C, Humidity = 20 to 80%
  • Capillary Array Lengths: 36 cm or 50 cm
  • Capillary Dimensions: 150 5m o.d. / 50 5m i.d.
  • Polymer Consumption
    • 36cm: approx. 200µl per run
    • 50cm: approx. 250µl per run

ABI 3730xl DNA Sequencer         

  • Optics
    • In-capillary detection by dual-side illumination.
    • Multiple fiter sets: Z, E, G5, G5-RCT, Any4Dyeset, Any5Dyeset and S (optional)
    • Backside thinned CCD for maximum optical sensitivity
  • Capillaries
    • Choice of capillary lengths, 36cm or 50cm
    • Dimensions: 150µm o.d./50µm i.d.
  • Reagent Handling
    • Automated polymer replenishment pump for increase throughput
    • POP-7 performance robust polymer separation matrix
    • 5 sequencing run modules, 1 fragment analysis run and SNPlex run module
    • On-board reagents for up to 48-hours of unattended operation
  • Performance Per Run Type  
    • LongSeq: 1152 samples per day (980 Kbases)
    • StdSeq: 2304 samples per day (1550 Kbases)
    • FastSeq: 2304 samples per day (1600 Kbases)
    • RapidSeq: 3840 samples per day (2100 Kbases)
    • GeneMapper Software
      • 4224 samples per day (92,000 genotypes)
      • 36 cm, up to 500 bp resolution with 0.15 bp sizing precision in 34 minutes

 

Power Requirements       

  • Voltage: 200-220 volt or 230-240 volt

System Dimensions        

  • Width 100 cm
  • Depth 73 cm
  • Height 89 cm
  • Weight 180 kg
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Illumina HiSeq 2500 Genome Sequencer

Illumina HiSeq 2500 Genome Sequencer

Cambridge ID #: 19391

Description:

The HiSeq 2500 system is a high throughput sequencing system that combines scalable output with flexible (dual) run modes which supports multiple applications, and superior turnaround times. With rapid or high-output modes and single or dual flow cell options, the HiSeq 2500 can be tuned to meet output needs ranging from 9 Gb to 1 Tb or 300 million to 4 billion reads per run. This system is a 2014 model that has recently been deinstalled by Illumina and includes a cBot. It can run the V3 high output chemistry as-is, or V4 with a hardware upgrade from Illumina.  This can also run the most recent version of the rapid run chemistry.

Specifications:

  • Dimensions - W x D x H: 118.6 cm x 76.0 cm x 94.0 cm (46.7 in x 30.0 in x 37.0 in)
  • Weight: 221.4 kg (488 lbs)
  • Dual mode processing (High Output and Rapid Run)
  • HiSeq Control Software
  • Instrument Control Computer                       
  • Base Unit: Intel Xeon CPU E5-2620 @ 2.00 GHz (2 processors)
  • Memory: 64 GB RAM
  • Hard Drive: 8 x 1.0 TB 3.5” Enterprise SATA
  • Operating System: Microsoft Windows 7
  • Power Requirements: 100 - 240V AC 50/60Hz, 20A, 1500 W
  • Instrument Configuration: Computer and touch screen display Installation setup and accessories Data collection and analysis software
  • Laser: 532 nm, 660 nm, 650 nm

 

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Perkin Elmer DNA PCR / Thermal Cyclers

Cambridge ID #: 6758

Description:

The Perkin Elmer Cetus DNA Thermal Cycler allows for flexible temperature programming, step duration and rapid heating and cooling from step to step for consistent results. Features a 48-position aluminum block for 0.5ml GeneAmp Reaction Tubes.  Includes four pre-programmed files: Soak, Time Delay, Thermo-Cycle and Step-Cycle files and 2 demonstration files: Gene-Amp Thermo-cycle and Gene-Amp Step-cycle (93 programmable user files are available). File modifications include, Automatic Segment Extension, Infinite Post-PCR Subambient Incubation.

Specifications:

  • Dimensions (H x W x D): 12" x 13" x 19.25"
  • Power Req: 120 V, 50/60 Hz, 1000 W
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Bio-Rad DNA Tetrad 2 / PTC-240 PCR / Thermal Cyclers

Bio-Rad DNA Tetrad 2 / PTC-240 PCR / Thermal Cyclers

Cambridge Scientific ID #'s: 19287, 8607

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

  • High-throughput cycling in a compact footprint
  • Intuitive graphical interface for rapid input of protocols and easy file management
  • Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
  • Excellent temperature accuracy and uniformity for consistent results
  • Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
  • Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

  • Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
  • Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
  • High-throughput 384-well unit
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MJ Research DNA Tetrad 2 / PTC-240 PCR / Thermal Cyclers

MJ Research DNA Tetrad 2 / PTC-240 PCR / Thermal Cyclers

Cambridge Scientific ID #'s: 14629, 9372, 13876, 10213

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

  • High-throughput cycling in a compact footprint
  • Intuitive graphical interface for rapid input of protocols and easy file management
  • Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
  • Excellent temperature accuracy and uniformity for consistent results
  • Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
  • Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

  • Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
  • Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
  • High-throughput 384-well unit
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Perkin Elmer DNA Thermal Cycler PCR / Thermal Cyclers

Cambridge ID #: 12487

The DNA Thermal Cycler 480 automates the polymerase chain reaction (PCR) technique for amplifying DNA.

Specifications:

  • Capacity: 48 x 0.5 mL
  • Temperature Range: -5°C to 100°C
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Macconnell MP4800 DNA Purifier System

Macconnell MP4800 DNA Purifier System

Cambridge ID #: 20049

Features:

 

Mini 48 DNA purification instrument is used to automatically purify plasmid DNA directly from bacterial culture in just over one hour.



Specifications:

 

 

  • Electrical: 110V

 

 

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Agilent 2100 (G2938A) Bioanalyzer

Agilent 2100 (G2938A) Bioanalyzer

Cambridge ID #: 8388

Includes:

  • Agilent G2938A Bioanalyzer
  • Computer with Bioanalyzer Software
  • Vortexer
  • Priming Station

 

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Results are delivered within 30-40 minutes in automated,

high quality digital data.

 

Miniaturization of analytical instrumentation has many advantages over conventional techniques. These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows

 

Areas of Application:

  • On-chip flow cytometry
    • The easy way to acquire dual-color, cell-based fluorescence data
  • RNA quality check with RIN
    • The industry standard for RNA analysis offering total RNA, mRNA and Small RNA's data including RIN algorithm (RNA Integrity Number)
  • DNA size and quantity
    • Smart, high resolution separation and quantification of DNA Highly sensitive DNA sample QC for next generation sequencing experiments
  • SDS-PAGE replacement for protein analysis
    • The fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity

 

Features:

  • Faster than gels – analyze 10-12 samples in 30-40 minutes
  • Easy to use – load the chip, press "start“, the instrument does the rest
  • Ready-to-use assays and pre-packaged reagent kits
  • Reproducible data, contamination-free hardware
  • Quick and easy sample comparison – one-click overlay, scaling or zooming features
  • Reproducible, complete and digital data
  • Easy handling, storage and exchange of digital data
  • Quantification of each individual fragment per sample offers purity results 2-color analysis of fluorescently stained cells
  • Data is acquired and archived in digital format.
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Agilent 2100 (G2938B) Bioanalyzer

Agilent 2100 (G2938B) Bioanalyzer

Cambridge ID #: 16169

Includes:

  • Agilent G2938C Bioanalyzer
  • Computer with Bioanalyzer Software
  • Vortexer
  • Priming Station

 

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA, RNA, proteins and cells. Results are delivered within 30-40 minutes in automated,

high quality digital data.

 

Miniaturization of analytical instrumentation has many advantages over conventional techniques. These advantages include improved data precision and reproducibility, short analysis times, minimal sample consumption, improved automation and integration of complex workflows

 

Areas of Application:

  • On-chip flow cytometry
    • The easy way to acquire dual-color, cell-based fluorescence data
  • RNA quality check with RIN
    • The industry standard for RNA analysis offering total RNA, mRNA and Small RNA's data including RIN algorithm (RNA Integrity Number)
  • DNA size and quantity
    • Smart, high resolution separation and quantification of DNA Highly sensitive DNA sample QC for next generation sequencing experiments
  • SDS-PAGE replacement for protein analysis
    • The fast and reliable way to determine the quantity and purity of proteins from Coomassie down to silver stain sensitivity

 

Features:

  • Faster than gels – analyze 10-12 samples in 30-40 minutes
  • Easy to use – load the chip, press "start“, the instrument does the rest
  • Ready-to-use assays and pre-packaged reagent kits
  • Reproducible data, contamination-free hardware
  • Quick and easy sample comparison – one-click overlay, scaling or zooming features
  • Reproducible, complete and digital data
  • Easy handling, storage and exchange of digital data
  • Quantification of each individual fragment per sample offers purity results 2-color analysis of fluorescently stained cells
  • Data is acquired and archived in digital format.
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Applied Biosystems 3130xl Genetic Analyzer Genetic Analyzer

Applied Biosystems 3130xl Genetic Analyzer Genetic Analyzer

Cambridge ID #: 19975

Features:

Applied Biosystems 3130 (4-capillary) Genetic Analyzer was refurbished by the original manufacturer to meet both manufacturing and product specifications. Availability may be limited.

Applied Biosystems 3130 Genetic Analyzer is the latest generation of 4-capillary electrophoresis instruments for the low to medium throughput laboratories. The system offers industry-leading performance, plus sophisticated automation capabilities allowing you to save time, reduce costs and increase productivity.

Specifications:

  • Capillary Length: 22cm, 36cm, 50 cm, 80cm
  • Current Limit: 15 A
  • Dimensions: Depth: 55cm, Height: 81cm, Width (open): 149cm
  • For Use With (Equipment): 3130 Genetic Analyzer
  • Format: 384-well plate, 96-well plate
  • Number of Capillaries: 4 Capillaries
  • Operating Environment:Humidity: 20%-80%, Temperature: 15°C-30°C
  • Operating System: Microsoft Windows 7
  • Performance: 98.5% base calling accuracy
  • Polymer Consumption: Up to 960 samples per 7000µl Bottle
  • Product Size: 1 instrument
  • Read Length: Up to 950bp
  • Sample Volume: > 10µL
  • Throughput: 30,400 bases/24hr (Long Read Sequencing), 4,320 genotypes/24hr (4-dye DNA Sizing), 5,760 genotypes/24hr (5-dye DNA sizing), 82,000 bases/24hr (Ultra Rapid Sequencing)
  • Voltage Limit: 200-220V or 230-240V
  • Weight: 130 kg
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Lonza 57025 FlashGel System

Lonza 57025 FlashGel System

Cambridge ID #: 20076

Features:

 

The FlashGel System for fast electrophoresis of DNA is the ideal sample screening tool. Check up to 32 PCR or restriction fragments quickly without having to plan your day around agarose gels or verify your DNA sample integrity prior to downstream analysis. The FlashGel System is the fastest way to separate DNA and the best way to watch DNA migration as it happens. This revolutionary new tool separates DNA in 2 - 7 minutes. Monitor gel runs right at the bench, without UV light. The highly sensitive system allows a 5-20X reduction in DNA levels - saving both time and money.

 

Specifications:

 

 

  • 5 minute separation and recovery
  • See bands in as little as 2 minutes
  • Recover samples directly without UV light band excision or purification,
  • Real-time separation and documentation
  • Watch band migration as it happens
  • Photograph gels at the bench without DNA damaging UV light
  • Outstanding sensitivity and resolution

 

 

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Lonza 57040 FlashGel System

Lonza 57040 FlashGel System

Cambridge ID #: 20077

Features:

 

The FlashGel Camera captures data from the FlashGel System and say goodbye to darkrooms and UV light. Complete separation and documentation safely, at your bench, in minutes. This simple digital camera in an enclosed hood connects directly to your laptop or PC via USB cable.

 

Specifications:

 

 

  • 5 minute separation and recovery, See bands in as little as 2 minutes
  • Recover samples directly without UV light band excision or purification
  • Real-time separation and documentation
  • Watch band migration as it happens
  • Photograph gels at the bench without DNA damaging UV light
  • Outstanding sensitivity and resolution

 

 

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VWR Accupower 500-2 Electrophoresis Power Supply

Cambridge ID #: 6545

Ideal for most applications including protein mini-gels, SDS-PAGE, and DNA electrophoresis.

Specifications:

 

  • Temperature Range: 0 to 40 °C
  • Dimensions: 10” D x 7.5” W x 4” H
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VWR AccuPower Model 300 Electrophoresis Power Supply

Cambridge ID #: 7703

Ideal for most applications including protein mini-gels, SDS-PAGE, and DNA electrophoresis.

Specifications:

 

  • Temperature Range: 0 to 40 °C
  • Dimensions: 10” D x 7.5” W x 4” H
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MJ Research ALD-1234 Alpha Blocks

Cambridge Scientific ID #'s: 8483, 7829

Description:

The MJ Research Alpha Slide ALD-0211 Thermal Cycler is engineered to adapt to the diverse needs of modern laboratories. This thermal cycler is designed as a swappable sample-block/heat-pump assembly, capable of customizing any DNA Engine line thermal cycler. The ALD-0211 is equipped with a thermal cycler base which efficiently distributes electric power, as well as thermoelectric heat pumps and sensors which help control sample temperatures. The ALD-0211 Thermal Cycler meets NIST-traceable standards with its ability to provide outstanding temperature uniformity using multi-sensor, multi zone temperature controls. Designed for humidified chamber hybridizations and in situ procedures, the Alpha Slide ALD-0211 system thermally cycles glass microscope slides in a dual-block format, with each block accommodating up to 16 slides.

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