Used Lab Equipment

Description:

Built on more than 25 years of BD experience and leadership in flow cytometry and multicolor analysis, the BD FACSCanto™ II system is an easy-to-use benchtop analyzer that delivers proven performance, accuracy, and high-quality results. The BD FACSCanto II can be configured with two or three lasers to detect up to eight colors. It features many innovations, including a true fixed alignment flow cell to minimize startup time and improve reproducibility. The optical system maximizes signal detection and increases sensitivity and resolution for each color in a multicolor assay. These and other capabilities make the BD FACSCanto II ideal for today’s busy clinical lab, providing a high degree of automation and quality control. With optimal reproducibility, the BD FACSCanto II system reduces hands-on technician time and costs associated with repeat testing.

Specifications:

• Carryover: ≤0.1%
• Sample Injection: Direct into flow cell
• Max Particle Size: 50 μm
• Sample Flow Rate, Min: 10 μL/min
• Sample Flow Rate, Max: 120 μL/min
• Sample Acquisition Rate: 10,000 events/second, 6 compensated fluorescence parameters and 2 scatter parameters
• Sample Dead Volume: 30 μL
• Sheath Consumption: 1.10 L/h normal operation; <1 mL/h standby

Features:

• BD CSampler- 96 well plate and 24 tube rack automated sampler for BD Accuri C6 flow cytometer  
• Blue and red laser
• Two light scatter detectors
• Optical filters such as FITC, PE, PerCP and APC
• A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use
• Optional filters and the Selectable Laser Module expand the available fluorochrome combinations
• Compact system and portable weight (13.6 Kg)
• Intuitive interface of the software
• Measures: 11 x 14.75 x 16.5 inches (H x W x D) (27.9 x 37.5 x 41.9 cm)

Recent PM Guaranteed Installable:

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software
• Fluids Cart
• 3 laser, Blue(488) and Red (633) and Violet (405)
• 8 PMTs in 4-2-2 configuration w/ side scatter
• All Necessary Cables
• Manuals

Optics

Lasers

• Air-cooled
• 488-nm solid state, 20-mW laser output
• 633-nm HeNe, 17-mW laser output
• 405 nm Violet laser
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 0 μm
• SSC Resolution: 5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP
• Fluorescence Detectors: 8 PMTs in 4-2-2 configuration w/ side scatter

The Millipore Guava EasyCyte HT offers sample handling formats for both tubes and 96-well plates, direct sampling using patented microcapillary system, and a user-friendly, versatile software. EasyCyte instruments are also compatible with many fluorochromes and fluorescent reagents.

Specifications:

• Operating temperature: 16°–35°C (60°–95°F)
• Operating humidity: 10–90% relative humidity (non-condensing)
• Power: 100–240 VAC, 50/60 Hz, 120W
• Dimensions: 21 x 24 x 12 inches
• Weight: 71lb
• Detection: 5 colors including forward scatter
• Laser: blue 488 nm (50mW)
• Flow cell dimension: standard square capillary with ID of 100 µm
• Pump: 7 µL/min to 36 µL/min
• Sample flow rate: positive displacement
• Waste and cleaning vials: 30-mL glass vial with screw top
• Waste generation: typically <40 mL in 8 hours of continuous use
• Dead volume: 50 µL (for 96-well microplate); 75 µL (for 0.5-mL microcentrifuge tubes)
• Sample concentration: final particle concentration of 104 to 5 x 105 particles/ml
• Sample requirement: for accurate results (up to 2.5 x 106 beads/mL for the RapidQuant Assay) as few as 2,000 cells/test; typically 25,000-100,000 cells/test, depending on the assay
• Data management: Output data file formats - (1) binary data storage in FCS 3.0 format; (2) spreadsheet results file in CSV format

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software
• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• 3 PMTs in 4-2 configuration w/ side scatter
• All Necessary Cables
• Manuals

Optics

Lasers

• Air-cooled
• 488-nm solid state, 20-mW laser output
• 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 1.0 μm
• SSC Resolution: 0.5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP

• Fluorescence Detectors: 6 PMTs in 4-2 configuration w/ side scatter

Single Laser Configuration

Includes Computer Loaded with Cytosoft Software

The guava easyCyte flow cytometry systems are cost-effective, easy to use and deliver complete and comprehensive cell analysis- right on your benchtop. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers—all while providing the analytical power you need to expand your research horizons.

Features:

• Blue Laser
• Forward and Side Scatter
• Tube Sampling
• Automated 96 Well Micrplate Sampling
• Small Sample Size (<20 μL)
• Minimal waste (<50 mL per day)
• Detectors
  • Green
  • Yellow
  • Red

Fluidics Specifications:

• Flow Cell Dimension: Standard square capillary with ID of 100 µm
• Pump: Positive displacement
• Sheath Fluid: None
• Sample Flow Rate: 7µL/min to 72 µL/min
• Waste Generation: typically <40mL in 8 hours of continuous use
• Dead Volume: 50 µL (for 96-well microplate)
• Sample Requirement: as few as 2,000 cells/test; typically 25,000 – 100,000 cells
• Cell Size: 1-60 µm
• Typical # of Cells/Protocol: 1,000 – 10,000 cells/test

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software

• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• Laser
• 6 PMTs unit and side scatter

• All Necessary Cables
• Manuals

Optics Specifications:

• Lasers
  • Air-cooled:
  • 488-nm solid state, 20-mW laser output
  • 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 0 μm
• SSC Resolution: 5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP
• Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
• Blue Laser Dyes
  • FITC, PE, PerCP or PerCP-Cy™5.5,
  • PE-Cy7 (525, 575, 678 or 695, 785 nm)
• Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
• Detector Bands
  • Blue Laser: 530/30; 585/42; >670; 780/60 nm
  • Red Laser: 660/20; 780/60 nm
• Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF