Used Lab Equipment

Features:

• Olympus BX60 Fluorescence Trinocular Microscope
• (2) WH10x-H/22 Eyepieces
• U-AC abbe condenser
• Mechanical Stage
• (2) 100W halogen lamphouses
• Objectives        
  • UplanFL 10x/.30
  • UPlanFL 20x/.50
  • UPlanFL 40x/ .75
  • UPlanFL 100x/ 1.30 Oil
• Reflective Cube

 Additional Objectives and Configurations Available.  Cameras are also available.

Features: 

• 20-MHz readout: High-speed, high-sensitivity image capture
• 2048 x 2048 imaging array: Resolves fine detail
• 7.4 x 7.4-ìm pixels: Ideally matched to optical microscope
• Interline-transfer, progressive-scan CCD: Eliminates camera vibration and facilitates fast triggering
• Flexible binning and readout: Increases light sensitivity while increasing the frame rate
• 12-bit digitization: Quantifies bright and dim signals in the same image
• Thermoelectric cooling: Long integration times for higher sensitivity
• Enhanced quantum efficiency: Provides higher sensitivity than typical interline cameras
• C-mount: Easily attaches to microscopes, standard lenses, or optical equipment
• Acquisition software: Captures, analyzes, and saves high-resolution images
• PCI interface: High-bandwidth, uninterrupted data transfer
• PVCam, Circular buffers, Device sequencing: Supported by numerous third-party software packages, Real-time focus, Precise integration with shutters, filter wheels, etc. (Compatible with Windows XP/Vista 32, Mac OS X, and Linux (kernel versions 2.4 and 2.6.8))
• CCD image sensor: Kodak KAI-4020M; interline-transfer, progressive-scan device with microlenses
• CCD format: 2048 x 2048 imaging array, 7.4 x 7.4-um pixels, 15.16 x 15.16-mm imaging area (optically centered)
• Linear full well: 30,000 e- (single pixel); 60,000 e- (2 x 2 binned pixel)
• Read noise: ≤10 e- rms @ 20 MHz
• Nonlinearity: <1%
• Digitizer type: 12 bits @ 20 MHz
• CCD temperature: -25ͦC (regulated)
• Dark current : 0.1 e-/p/s @ -25ͦC
• Operating environment: 0 to 30ͦC ambient, 0 to 80% relative humidity noncondensing
• I/O TTL (trigger/status): trigger, invert, inhibit, exposing, interline shift, frame readout, 8-bit TTL (programmable), 8-bit DACs(two)

Compare this against “Features of Gel Doc XRS+ system”: 

• Chemiluminescence: Yes
• Fluorescence: Yes
• Colorimetry/densitometry: Yes
• Gel documentation: Yes  

Hardware Specifications        

• Maximum sample size: 28 x 36 cm
• Maximum image area: 26X 35 cm
• Excitation source: Trans-UV and epi-white are standard (302 nm included, with 254 and 365 nm available as options)
• Illumination control: 3 modes (trans-UV, trans white, epi-white)
• Detector: super cooled CCD
• Image resolution: 4 megapixels
• Pixel size (H x V): 6.45 x 6.45 µm
• Filter holder: 3 positions (2 for filters, 1 without filter)
• Emission filters: 1 included (standard), 3 optional
• Dynamic range: >4.0 orders of magnitude
• Pixel density (gray levels): 65,535
• Dynamic flat fielding: Application specific, for all applications
• Instrument size (L x W x H): 36 x 60 x 96 cm
• Instrument weight: 32 kg

 Operating Ranges         

• Operating voltage: 110/115/230 V AC nominal
• Operating temperature: 10–28°C (21°C recommended)
• Operating humidity: <70% noncondensing

 Automation Capabilities            

• Workflow automated selection: Application driven; user selected or recalled by a protocol
• Workflow automated execution: Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
• Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
• Autofocus: Precalibrated focus for any zoom setting or sample height
• Image flat fielding: Dynamic; precalibrated and optimized per application
• Autoexposure: 2 user-defined modes (intense or faint bands)

Includes:

• Cabinet & Camera
• Computer With Software
• Manual

The Bio-Rad ChemiDoc XRS System is Bio-Rad’s most advanced imaging system. The system is able to image chemiluminescent, fluorescent and colorimetric samples and comes complete with a compact darkroom, transilluminator and supercooled high resolution 1.44 megapixel CCD camera. The entire system is controlled by the provided Quantity One 1-D analysis software with additional controls on the compact darkroom to control focus, aperture and zoom. Consequently, the ChemiDoc XRS system is extremely flexible and can be used for imaging a diverse range of applications such as Western blotting, DNA/RNA gel electrophoresis, 2-D gel electrophoresis, densitometry, etc. The system is able to detect HRP and AP chemiluminescence, ethidium bromide, SYBR Green, green fluorescent protein, silver stain and Coomasie brilliant blue staining to name but a few that are frequently used with the above applications. The system features flat fielding technology which allows for superior uniformity and quantification. Flat-fielding is a technique that corrects for pixel-to-pixel variations by dividing the gain variations out of an image, thus flattening the relative response for each pixel.

Operation of the system is very simple. The gel or membrane is placed on the transilluminator, which is set into a drawer which can be moved in and out of the darkroom, facilitating placement of the gel or membrane. Once the gel or membrane is placed, it and the transilluminator are returned to the darkroom and the door closed. With the Epi-illumination switched on, the aperture, zoom and focus are adjusted using the touchpad on the darkroom and viewed on the computer monitor until the desired position, size and focus of the gel are achieved. To acquire the image, the appropriate light source for the gel or membrane is selected and the software is used to select an integration time. Using the Quantity One software, the image is frozen when a satisfactory image is acquired. The Quantity One 1-D image analysis software aids the user in obtaining the best images possible from the gel or membrane. The software is user-friendly and equipped with built-in quick guides and wizards to facilitate the optimization of images and printouts. The software is compatible with both Microsoft Windows and Macintosh platforms. The Quantity One 1-D software is used for analysis of 1-D electrophoresis gels, Western blotting membranes, Coomassie-stained SDS-PAGE gels, etc. whereas the PDQuest 2-D analysis software is also available and is intended to be used for comparative analysis where many 2-D gels are analyzed at once.

Specifications:

• CCD Resolution (HxV): 1,392 x 1,040µm
• Pixel density: 65,536 gray levels
• Imaging area: 25 x 26 cm
• Camera cooling temperature: -50°C
• Camera cooling system: Peltier
• Dynamic range: 4 orders of magnitude
• Motorized zoom lens: C-mount, f/1.2, 12-75 mm
• Illumination modes: Epi-white, trans-UV (302 nm), and white

Using advanced electrically cooled SuperChemiNova CCD camera technology, the FluorChem provides real time display and the R.S.D.P. standard of resolution, sensitivity, dynamic range and performance. The FluorChem is ideal for chemiluminescence, fluorescence, and visible imaging applications.

Features:

• Applications: chemiluminescence, fluorescence and colorimetric
• Transillumination White: For protein gels,autorads,film, plates and flasks
• Transillumination UV: For the fluorescent gels such as EtBr, SYPRO Red, etc
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: High-performance, high resolution, CCD camera allows imaging of low-light samples in UV-illuminated, and fluorescent applications. 12.5x75mm, F1.2 Motorized Zoom Lens for ChemiImager 5500 with DE 500/400 cabinet (Includes motorized lens, ChemiImager 5500 camera mount, and cables. Requires AlphaEaseFC software version with software controls of motorized lens) -
• Lens: Zoom lens, close-up 2+ diopter lens and interference filter
• Filters: chemiluminescence samples, ethidium bromide, SYPRO Orange (595 nm) each filter has a bandwith +/-40 nm
• Allows to visualize chemiluminscent reactions

How is this unit better than Alpha Imager 3400:

For low-light applications, such as chemiluminescence, a longer exposure time may be appropriate and it is best to go to the red ACQUIRE IMAGE button to directly acquire the image once the desired exposure time is set. Alternatively, if a good exposure time is unknown, try selecting Auto-Expose and using the super sensitivity selection. Auto-Expose will generate an image showing the signal intensity usually in less than 3 minutes. Afterwards, a different sensitivity (binning) mode can be selected to generate a better resolution image and the software will automatically calculate the new exposure time for you.

With improved image quality and ergonomics, the Olympus CKX53 inverted microscope delivers stable performance and a comfortable workflow for a variety of cell culture needs, including live cell observation, cell sampling and handling, image capture, and fluorescence observation.

Specifications:

• Observation Method: Fluorescence (Blue/Green Excitation), Fluorescence (Ultraviolet Excitation), Phase Contrast, Brightfield
• Revolving Nosepiece(Manual, Standard Type): Built in, 4 positions
• Observation Tubes: Widefield (FN 22), Trinocular
• Illuminator: Transmitted Illuminator, LED Lamp, Fluorescence Illuminator, 100 W Mercury Lamp, Light Guide Illumination
• Stage: Manual, Plain Stage
• Condenser(Manual, Ultra-Long Working Distance Condenser): NA 0.3/ W.D. 72 mm (built in)
• Dimensions (W×D×H): 200 mm ×498 mm ×454 mm (7.9 in. ×19.6 in. ×17.9 in.) (Phase contrast entry configuration)
• Weight: Approx. 6.9 kg (15.2 lb)

High-quality family of climatic boxes with active simulation of temperature, humidity and lighting option and chamber volume 222 liters. User-friendly control using the touch display, integrated data logger, multi-language communication, 100 programs – each of them with up to 100 segments offering unique interface suitable for simple applications and for demanding users as well. Traditional fluorescence or more powerful LED lighting placed in the doors or in the racks of the device, adjustable lighting intensity in 1% steps, optional spectrum of lighting supporting day and night simulation in any location of the world or testing of stability or ageing of materials in industrial locations (option).

Specifications:

• Interior of stainless steel material: volume (222 l), depth (530 mm), width (540 mm), height (765 mm)
• Volume of the steam space: 305 l
• Working temperature (from 0.0 to): 100 °C
• Heat emission at 37 °C: 63 W
• Outer dimensions: depth (885 mm), width (780 mm), height (1450 mm)
• Weight: 263 kg (brutt cca)

Includes:

• 50x and 100x Objectives
• 10x/25 Eyepieces

• 6x6" sample area with a 6x6" Marzhauser motorized stage

• Easily upgradable to DIC and Polarized Light
• Additional Objectives available.

The modular design of the automated Leica DM6000 microscope for materials and geo science makes it possible to configure a system for every user’s specific application and preference.

The microscope can be used with all common incident light methods (brightfield, darkfield, polarization, interference contrast, fluorescence contrast), all fully automated on request. The incident light axis also has a 4x reflector disk with 2 permanently mounted positions and 2 interchangeable positions for adding reflectors or fluorescence filter cubes, which you can select from a wide range of accessories.

Features:

• Fully motorized and encoded microscope
• 6x6" sample area with a 6x6" Marzhauser motorized stage
• Complete with BF/DF/DIC/POL with a 1.25x, 10x, 20x, 50x objectives, trinocular head and eyepieces.  

• Perfectly tailored system for research tasks – with high-resolution digital cameras and software modules for image analysis and archiving
• Fully automated incident light axis for brightfield, darkfield, polarization, interference contrast, and fluorescence
• Incident light with 4-position reflector disk (two fixed positions, two variable positions) for reflectors or fluorescence filter cubes
• Automated illumination manager and contrast manager, and fully motorized aperture and field diaphragms
• Motorized z focus drive and motorized stages, reproducible x, y, and z positions
• Motorized, encoded 6-position objective nosepiece
• One-of-a-kind memory function for simultaneous changeover of the objective and contrast method
• Integrated Leica SmartTouch panel for control and monitoring of all automated components

• Research upright
• Phase Contrast
• Fluorescence
• Imaging
• 10x ph1
• 40x ph2
• 63x
• 100x

Description:

The Eclipse 90i delivers a completely integrated, totally optimized digital microscopy system engineered to master the most demanding applications. The system is equipped with a broad range of designed-for-digital CFI Plan Apo VC objectives, delivering incredibly crisp, clean and true-to-life images. Combine this with the 90i's exclusive motorized digital head, proprietary "fly-eye" lens array, and integrated epi-fluorescence attachment, and you have a motorized microscope system geared to take the work out of adjusting the microscope so you can spend more time on what really matters – your research.

Specifications:

• Optical system: CFI60 infinity optical system
• Illumination:
• Precentered 12V100W halogen transmitted illumination
• Built-in fly-eye lens
• Built-in NCB11/ ND8/ ND32 filters & diffuser
• Preset switch
• Motorized field diaphragm
• External power supply
• Focusing:
• Motorized coaxial coarse/fine/super-fine focusing
• Built-in linear encoder, resolution : 0.05um
• Focusing stroke : 27mm
• Escape function as refocusing mechanism
• Auto Link Focus
• Description:
• The Eclipse 90i delivers a completely integrated, totally optimized digital microscopy system engineered to master the most demanding applications. The system is equipped with a broad range of designed-for-digital CFI Plan Apo VC objectives, delivering incredibly crisp, clean and true-to-life images. Combine this with the 90i's exclusive motorized digital head, proprietary "fly-eye" lens array, and integrated epi-fluorescence attachment, and you have a motorized microscope system geared to take the work out of adjusting the microscope so you can spend more time on what really matters – your research.
• Specifications:
• Optical system: CFI60 infinity optical system
• Illumination:
• Precentered 12V100W halogen transmitted illumination
• Built-in fly-eye lens
• Built-in NCB11/ ND8/ ND32 filters & diffuser
• Preset switch
• Motorized field diaphragm
• External power supply
• Focusing:
• Motorized coaxial coarse/fine/super-fine focusing
• Built-in linear encoder, resolution : 0.05um
• Focusing stroke : 27mm
• Escape function as refocusing mechanism
• Auto Link Focus
• Filter Cubes
• Cy5, DAPI, FITC, CY3
• Objectives:
• 2x   planApo
• 4x    planApo
• 10x   planApo
• 20x   planApo
• 40x  planApo

• Camera Nikon DSRi1
• Windows 7, 32 bit CPU preload with Micromanager 
• NEW* Excelitas X-Cite Xylis XT720S Microscope LED Fluorescence light Source 

Includes:

• Trinocular Head
• (2) 10X/25 Eyepieces
• Objectives
  • Plan Flour 10X
  • Plan Flour 20X
  • Plan Fluor 40X
  • Plan Flour 100X oil
• Camera Port
• Fluorescence
  • Fitc, Dapi, Cy3
• 100mg Fluorescent light w/ power supply OR Xcite 120Q Metal Halide OR Xcite 120LED

Additional Objectives and Configurations Available. Cameras are also available.

Specifications:

• Dual fluorescence filters manual
  • Upper Thor laser
• 40x PA .95NA objective with correction collar
• Phase condenser
• Nikon Perfect Focus 2
• Prior H117EINS motorized X/Y stage with Proscan 3 controller
• Photometrics 95B digital Camera with 1x c-mount adapter (left side)
• Lumencor Solar Epi Light source lower fluorescence turret 535nm cube
• Right Port Thor SM1CP2 Nikon Beam splitter with eyeview USB 3 digital camera
  • Launch to 32 fiber optics with Thor CB74EX UK 6 axis manipulator for launch alignment
  • 2x Hamamatsu 8 channel PMT detectors H11452-20 PA0347
  • 16x Thor EF112 Low Pass Filter
  • National Instruments BNC-2110 controller with USB switching
• Coherent OBIS 488 Laser Mod 150nm to Thor launch
• Thor laser expander with manual Z axis control
• West Pro-EC44 temp control
• Custom climate controller
• i7-7700 CPU 3.60GHz PC 16gb ram
  • Micro-Manager
  • Stage Auto
  • Genturi Viper
  • Pulsed AO
• 20x PA .75NA objective
• 20x Splan FL .7 objective with correction
• 10x Fluor objective
• Newport Isolation anti-vibration 3’ / 4’ Tables with blackened enclosures access doors in front and rear including laser safety switch

Specifications:

• Dual fluorescence filters manual
  • Upper Thor laser
• 40x PA .95NA objective with correction collar
• Phase condenser
• Nikon Perfect Focus 2
• Prior H117EINS motorized X/Y stage with Proscan 3 controller
• Photometrics 95B digital Camera with 1x c-mount adapter (left side)
• Lumencor Solar Epi Light source lower fluorescence turret 535nm cube
• Right Port Thor SM1CP2 Nikon Beam splitter with eyeview USB 3 digital camera
  • Launch to 32 fiber optics with Thor CB74EX UK 6 axis manipulator for launch alignment
  • 2x Hamamatsu 8 channel PMT detectors H11452-20 PA0347
  • 16x Thor EF112 Low Pass Filter
  • National Instruments BNC-2110 controller with USB switching
• Coherent OBIS 488 Laser Mod 150nm to Thor launch
• Thor laser expander with manual Z axis control
• West Pro-EC44 temp control
• Custom climate controller
• i7-7700 CPU 3.60GHz PC 16gb ram
  • Micro-Manager
  • Stage Auto
  • Genturi Viper
  • Pulsed AO
• 20x PA .75NA objective
• 20x Splan FL .7 objective with correction
• 10x Fluor objective
• Newport Isolation anti-vibration 3’ / 4’ Tables with blackened enclosures access doors in front and rear including laser safety switch

Specifications:

• Prior ES107NTE with Optiscan 3
• Fluorescence TRITC cube
• Photometrics Coolsnap DYNO
• Phase condenser
• Lumencor Solar Epi Light source lower fluorescence turret 535nm cube
• Coherent OBIS 488 Laser Mod 150nm to Thor launch
• 2x Plan UV , 10x Fluor, 40x PA or .95NA, 40x Fluor 1.3NA oil, 100x clone RMS 1.25 oil
• OKO Lab Stage top Incubator with controller 
• Newport Isolation anti-vibration 3’ / 4’ Tables with blackened enclosures access doors in front and rear including laser safety switch

Features:

The Leica EL6000 external light source for enhanced fluorescence imaging connects via a liquid light guide to all Leica Microsystems’ upright, inverted, and stereo microscopes. The Leica EL6000 keeps heat away from the specimen and microscope and uses an alignment-free, mercury metal halide bulb with an extremely long lifetime of more than 2000 hours. This reduces operating costs and saves the time it takes for centering lamp housings. The Leica EL6000 has an integrated fast shutter, which is fully supported by the Leica LAS AF software.

 Features:

• Applications: fluorescence and colorimetric
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: high sensitivity, high resolution scientific grade CCD camera with 2.0 megapixel resolution, extendable to 6.0 megapixels
• Lens:5-75 f/1.2
• Overhead Light Selector: White light, 365 nm UV, 254 nm UV
• Flters: ethidium bromide, SYBR Green, SYBR Gold
• Visible color stains: Coomassie, Silver
• Allows to visualize chemiluminscent reactions
• Manual lift platform, 3 emission filters, white light plate, overhead UV, white light and blue light
• Transilluminator: Choice of UV transilluminator models