Used Lab Equipment

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Includes PM From BD and Guaranteed Installable under BD Service Contract

New Flow Cell installed in September 2018

Also included are the excitation laser lines, laser powers and optical configuration.

The new BD FACSAria IIu cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria IIu cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria IIu cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cellsorting.

Includes:

• 4 lasers (375nm, 488nm, 405nm, 633nm)
• Blue 7PMTs, Red 3PMTs, Violet 3PMTs
• HP Computer w/ FACSDiva 8.0 Software
• ACDU
• 1 HP monitor
• Fluidics Cart
• Temperature Control
• Lauda Chiller
• Buffalo Whisper Aerosol Control
• Under BD service contract since installed in 2012

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software

• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• Laser
• 6 PMTs unit and side scatter

• All Necessary Cables
• Manuals

Optics Specifications:

• Lasers
  • Air-cooled:
  • 488-nm solid state, 20-mW laser output
  • 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 0 μm
• SSC Resolution: 5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP
• Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
• Blue Laser Dyes
  • FITC, PE, PerCP or PerCP-Cy™5.5,
  • PE-Cy7 (525, 575, 678 or 695, 785 nm)
• Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
• Detector Bands
  • Blue Laser: 530/30; 585/42; >670; 780/60 nm
  • Red Laser: 660/20; 780/60 nm
• Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software
• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• 3 PMTs in 4-2 configuration w/ side scatter
• All Necessary Cables
• Manuals

Optics

Lasers

• Air-cooled
• 488-nm solid state, 20-mW laser output
• 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 1.0 μm
• SSC Resolution: 0.5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP

• Fluorescence Detectors: 6 PMTs in 4-2 configuration w/ side scatter

Using advanced electrically cooled SuperChemiNova CCD camera technology, the FluorChem provides real time display and the R.S.D.P. standard of resolution, sensitivity, dynamic range and performance. The FluorChem is ideal for chemiluminescence, fluorescence, and visible imaging applications.

Features:

• Applications: chemiluminescence, fluorescence and colorimetric
• Transillumination White: For protein gels,autorads,film, plates and flasks
• Transillumination UV: For the fluorescent gels such as EtBr, SYPRO Red, etc
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: High-performance, high resolution, CCD camera allows imaging of low-light samples in UV-illuminated, and fluorescent applications. 12.5x75mm, F1.2 Motorized Zoom Lens for ChemiImager 5500 with DE 500/400 cabinet (Includes motorized lens, ChemiImager 5500 camera mount, and cables. Requires AlphaEaseFC software version with software controls of motorized lens) -
• Lens: Zoom lens, close-up 2+ diopter lens and interference filter
• Filters: chemiluminescence samples, ethidium bromide, SYPRO Orange (595 nm) each filter has a bandwith +/-40 nm
• Allows to visualize chemiluminscent reactions

How is this unit better than Alpha Imager 3400:

For low-light applications, such as chemiluminescence, a longer exposure time may be appropriate and it is best to go to the red ACQUIRE IMAGE button to directly acquire the image once the desired exposure time is set. Alternatively, if a good exposure time is unknown, try selecting Auto-Expose and using the super sensitivity selection. Auto-Expose will generate an image showing the signal intensity usually in less than 3 minutes. Afterwards, a different sensitivity (binning) mode can be selected to generate a better resolution image and the software will automatically calculate the new exposure time for you.

Features:

This spectrophotometer is a modular system that allows its user to interchange a versatile range of accessories to correspond with the characteristics of a given sample. It is equipped with limitless configuration of accessories to enhance the accuracy and speed of the user’s application.

Specifications:

• Dimensions (W x H x D): (30.6 in x 13.7 in x 40.2 in)
• Optics: All reflective optics for high sensitivity at all wavelengths, and for
  Microsamples
• Source: 450 W CW Ozone-free Xenon arc lamp (250 to 2500 nm)
• Excitation grating: 1200 groove/mm blazed at 330 nm
• Emission grating: 1200 groove/mm blazed at 500 nm
• Bandpass: 0 to 30 nm (single mono, 1200 gr/mm grating), continuously adjustable
                   0 to 15 nm (double mono, 1200 gr/mm grating), continuously adjustable
• Wavelength Accuracy: ± 0.5 nm
• Integration Time: 1 ms to 160 s
• Base detector: Photomultiplier R928P, spectral coverage 200 to 870 nm
• Reference Detector: UV enhanced silicon photodiode
• Water Raman S/N: > 30,000:1 RMS (> 15,000:1 FSD)
• Phosphorescence Option: < 10 μs to >10 s

The FluoroMax® series represents HORIBA's industry-leading fluorometer performance in a convenient, affordable, easy-to-use benchtop model. The FluoroMax® family, with its unique all reflective optics and photon counting was the first to bring the sensitivity of a modular fluorometer to a tabletop fluorescence instrument.

It’s a compact spectrofluorometer, yet offers the ultimate sensitivity in fluorescence investigations as well as features not found in most table-top fluorescence detection systems.

The FluoroMax® series consists of the FluoroMax Plus, the latest generation of the original, high performance tabletop fluorometer, which offers extended performance with detection of emission spectra out to 1700 nm and (TCSPC) Time Correlated Single Photon Counting lifetime measurements as short as 25ps.

The FluoroMax series spectrofluorometers are ideal for measuring solid and liquid samples.

Description:

The MyiQ real time PCR detection system offers an affordable solution for the detection of common fluorescent green dyes such as FAM and SYBR Green I. This system interfaces directly with the iCycler thermal cycler, offering superior features such as thermal gradient capabilities and Peltier-effect driven performance.

Specifications:

• Temperature Range: 4 - 100℃

• Temperature Uniformity: ± 0.4℃

• Heated Lid Temperature: up to 100℃

• Gradient Mode

• Gradient Accuracy: ± 0.4℃

• Row Uniformity: ± 0.4℃

• Gradient Range: 40 - 100℃

• Sample Capacity: 96 Wells

• Sample Size: 15 - 100 µl

• Dimensions (W x D x H): 29.2 x 58.4 x 38.7 cm

• Fluorescence Excitation Range: 475 - 495 nm

• Fluorescence Detection Range: 515 - 545 nm

• Dynamic Range: 9 orders of magnitude

Includes:

• Olympus IX51 Inverted Phase Contrast Microscope
• (2) 10x/22 Eyepieces
• Mechanical Stage
• Objectives
  • PhL Plan 4x DL
  • Uplan FI 10x/0.30 Ph1
  • LC Plan FI 20x/0.40 Ph1
  • LC Plan FI 40x/0.60 Ph2

Additional Objectives, Configurations, and Cameras Available

Andor’s new iXon Life EMCCD (Electron Multiplying CCD) platform is available exclusively for fluorescence microscopy applications and is engineered to deliver single photon sensitivity with absolutely unparalleled price/performance. The ideal detector to drive superlative performance from confocal spinning disk technology. Whilst affording superb confocality and low rates of phototoxicity, spinning disk experiments are inherently photon starved, by virtue of the photon rejection implicit to optical sectioning. The superior sensitivity of the iXon Life detector brings these low light images to life.

Specifications:

• Sensor Type: Back Illuminated, standard AR coated
• Active pixels (H x V): 1024 x 1024
• Pixel Size (H x V: µm): 13 x 13
• Image Area: 13.3 x 13.3 mm with 100% Fill Factor
• Active Area Pixel Well Depth (e-): 80,000 180,000
• Pixel Readout Rate
• Minimum Temperature, air cooled, ambient 20°C: 10 MHz -70ºC, 30 MHz•3 -55ºC
• Chiller Liquid Cooling, coolant @ 10°C, >0.75l/min: 10 MHz -80ºC, 30 MHz•3 -65ºC
• Thermostatic Precision: ± 0.01°C
• Triggering: Internal, External, External Start, External Exposure, Software Trigger
• System Window Type: UV-grade fused silica, Broadband Visible-Near Infrared, 0.5 degree wedge
• Digitization: 16-bit (at all speeds)
• PC Interface: USB 3.0•12
• Lens Mount: C-mount
• Dark Current and Background Events•4,5:
• Dark Current (e- /pixel/sec) @ -80°C: 0.00025
• Spurious Background (events/pix) @ 1000x gain / -80°C: 0.005
• Active Area Pixel Well Depth: 80,000 e-
• Gain Register Pixel Well Depth•6: 730,000 e-
• Read noise (e-) •7: <1
• Linearity•8: Better than 99.9%
• Vertical Clock Speed: 0.6 to 4.33 µs (user selectable)
• Timestamp accuracy: 10 ns

Andor’s new iXon Life EMCCD (Electron Multiplying CCD) platform is available exclusively for fluorescence microscopy applications and is engineered to deliver single photon sensitivity with absolutely unparalleled price/performance. The ideal detector to drive superlative performance from confocal spinning disk technology. Whilst affording superb confocality and low rates of phototoxicity, spinning disk experiments are inherently photon starved, by virtue of the photon rejection implicit to optical sectioning. The superior sensitivity of the iXon Life detector brings these low light images to life.

Specifications:

• Sensor Type: Back Illuminated, standard AR coated
• Active pixels (H x V): 512 x 512
• Pixel Size (H x V: µm): 16 x 16 μm
• Image Area: 8.2 x 8.2 mm with 100% Fill Factor
• Active Area Pixel Well Depth (e-): 80,000 180,000
• Pixel Readout Rate
• Minimum Temperature, air cooled, ambient 20°C: 10 MHz -70ºC, 17 MHz•3 -70ºC
• Chiller Liquid Cooling, coolant @ 10°C, >0.75l/min: 10 MHz -80ºC, 17 MHz•3 -80ºC
• Thermostatic Precision: ± 0.01°C
• Triggering: Internal, External, External Start, External Exposure, Software Trigger
• System Window Type: UV-grade fused silica, Broadband Visible-Near Infrared, 0.5 degree wedge
• Digitization: 16-bit (at all speeds)
• PC Interface: USB 2.0
• Lens Mount: C-mount
• Dark Current and Background Events•4,5:
• Dark Current (e- /pixel/sec) @ -80°C: 0.00030
• Spurious Background (events/pix) @ 1000x gain / -80°C: 0.0018
• Active Area Pixel Well Depth: 180,000 e
• Gain Register Pixel Well Depth•6: 800, 000 e
• Read noise (e-) •7: <1
• Linearity•8: Better than 99.9%
• Vertical Clock Speed: 0.3 to 3.3 µs (user selectable)
• Timestamp accuracy: 10 ns

Designed to give optimized performance in all major multilabel reading applications including reporter gene assays, cell applications, genotyping, enzyme assays, signal transduction, receptor ligand binding, imunnoassays, and quantification, also capable of fluorometry, fluorescence polarization, TR-fluorometry, luminometry, photometry, features 96-well, 384-well and 1536-well capabilities, high speed dual emission reading, high throughput light source, top and bottom reading modes for fluorescence, allows for adjustments of measurement height, configured with enhanced luminescence, and a bar code reader, comes with plate stacker unit with two 50-plate capacity stackers, a computer operating on Windows XP or Windows 7 OS with EnVision v1.12 software installed, monitor, keyboard and mouse, 100V/240V, 50/60Hz, 250VA.

Specifications:

• Maximum Speed: 12,100 rpm / 13,500 g
• Maximum Tube Size (Diameter x Length): 11mm x 38mm
• Electrical: 115 V, 60 Hz, 8 A
• Interlock: Timer Safety Interlock

Features:

A premium color camera for both brightfield and fluorescence

Specifications:

• Full color sensor
• 6.0MP resolution
• Large field of view
• 75 % Peak QE
• USB 3.0 Connection
• Compatible with Windows 7/8/10

SENTERRA Raman Microscope combines numerous novel and patented features such as fluorescence removal and on-demand confocal depth profiling. The most important innovation is certainly its continuous calibration method, as it eliminates daily calibrations with external standards, providing you reliable, reproducible measurements.

Specifications:

• High wavenumber accuracy with SureCal
• All-in-one, compact, confocal design
• Removal of fluorescence background with Concave Rubberband Correction
• Confocal depth profiling with FlexFocus
• Up to 4 excitation lasers
• Computer controlled XYZ mapping stages with autofocus

Includes:

• Computer Loaded with SoftMax Pro 5.4.1
• All Necessary Cables and Cords
• Manuals

The SPECTRAmax 250 microplate spectrophotometer has a xenon flash lamp and grating monochromator that facilitate direct UV measurement of DNA- RNA- and protein in microplates. Up to 96 assays can be run at one time using disposable or quartz microplates. The purity of DNA can be measured with A260/A280 ratios. Also- users can perform spectral scans from 250 nm to 750 nm to simplify qualitative analyses of numerous samples. The product reportedly reduces sample volumes in experiments because the assays are performed in a microplate instead of cuvettes. SOFTmax PRO microplate data- analysis software- for use with the SPECTRAmax 250- provides built-in spreadsheet capabilities for easy data analysis and reporting.

Specifications:

• Wavelength Range: 250-700 nm
• Photometric Range: 0.000 to 4.000 OD
• Wavelength Selection: Monochromator, tunable in 1-nm increments
• Photometric Linearity (405 nm): 0 to 3.000 OD
• Wavelength Bandwidth: 5 nm
• OD Resolution: 0.001 OD
• Endpoint Read Time: 9 seconds
• Temperature Range: Ambient to 45 C
• Kinetic Read Time: 9-second minimum interval
• Temperature Uniformity (Microplate): +/-0.5 C @ 37 C well-to-well