Used Lab Equipment

AKTA FPLC system is a fully automated liquid chromatography system designed for research scale purification of proteins, wherein proteins of various sizes can be readily purified using different kinds of columns.

Features:

• P-920 Pump
• UPC-900
• Frac-900 Fraction Collector
• INV-907 Injection Valve
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• Box 900: Storage compartment and Solvent Tray
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 
• Manuals

Description:

Single Path Monitor UV-1 is a dual beam flow through UV absorptiometer for monitoring the UV absorption of a flowing liquid at 254nm, 280 nm, or 405 nm, comes with the UV-1 control unit, optical unit, one 280 nm wavelength filter and one 280 nm converter, and with a flow cell with optical path length of either 3 mm or 10 mm.

The ACQUITY UPLC TUV Detector is a tunable, dual wavelength ultraviolet/visible (UV/Vis) detector that offers optimal linearity, resolution, and sensitivity for UPLC/UV separations.  It features uniquely designed, patented, light-guiding flow cell technology, low-noise performance (6 μAU), and support for data rates up to 80 Hz.  The ACQUITY UPLC TUV Detector, coupled with our innovative ACQUITY UPLC Column chemistries and separations modules, leverages ultra-low dwell volume and low-noise, high-speed detection to bring productivity, sensitivity, and resolution that are unprecedented in HPLC techniques being used in the laboratory today.

Specifications:

• Wavelength range: 190 to 700 nm
• Wavelength accuracy: ±1 nm
• Linearity range: Deviation at 2.5 AU ≤5%, propylparaben, at 257 nm
• Baseline noise:6 x 10^-3 AU, at 230 nm, 10 mm shunt cell, 1.0 second filter time constant
• Drift: ≤5.0 x 10^-4 AU/hour/°C at 230 nm
• Sampling rate: Up to 80 points/s
• Unattended operation: Leak sensors, full diagnostic data captured through console software
• Light source: Prealigned, intelligent technology Deuterium lamp
• Flow cell design: Light-guiding UPLC flow cell with intelligent chip technology
• Path length: 10 mm (analytical flow cell)
• Cell volume: 500 nL (analytical flow cell)
• Pressure limit: 1000 psi (all flow cells)
• Wetted materials: 316 stainless steel, fused silica, Teflon AF, PEEK (analytical flow cell)
• Optical bandwidth: 5 nm

Features:

• Loop volume 5-1000µl
• 96 vials 1.5 ml vial capacity
• A-900 autosampler designed for use with AKTA
• Syringes 100 - 1000 µl
• Built-in compressor

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

Features:

• Fraction collector
• pH Electrode
• UV Monitor
• Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

Features:

• Fraction collector
• pH Electrode
• UV Monitor
• Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

Features:

• Fraction collector
• pH Electrode
• UV Monitor
• Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Features:

• Simple column selection with huge built-in library
• Automatic titration of buffers across a broad pH range
• Quick and easy transfer of methods to production-scale
• UNICORN method wizard High performance purification and characterization
• State-of-the-art computer-controlled pumps, UV/conductance monitors, and fraction collector
• UV-Vis detection of up to three wavelengths simultaneous

Specifications:

• Flow rate: 0.01-100 ml/min
• Operating pressure max.: 10 MPa
• Tubing i.d. (flow path): 0.75 mm
• UV wavelength: 190-700 nm
• Flow cell path length: 2 mm
• pH monitoring: 2–12
• Buffer selection: 11 buffer inlets
• Relative humidity: 10%-95% (non condensing)
• pH Stability: 1-14
• Viscosity: max 5 cP
• Multiple wavelength detection: Yes, up to 3 wavelengths
• Multiple sample injection: Yes, up to 8 samples
• Autosampler injection: Optional
• Automatic buffer preparation: BufferPrep
• Pump type: Piston pump, metering type
• Application: Purification of microgram to gram quantities of protein
• Dimensions (W x H x D): 480 x 610 x 470 mm
• Power Requirements: 100-120/220-240 VAC, 600W

ÄKTAxpress Module

ÄKTA xpress is a chromatography system designed for automated, multistep protein purification of both single and multiple samples.

• Unattended purification: Built-in purification knowledge combines software, hardware, and prepacked columns to eliminate time-consuming manual tasks.
• Highest possible purity: Optimized protocols with up to four steps will deliver tagged proteins of near 100% purity. Built-in cleaning procedures between MAb samples to minimize carry over
• High productivity: Each module can purify up to four proteins. The modular design offers adaptability to meet increased throughput needs.
• Yield : The system is capable of purifying target proteins in yields varying from 1 to 50+ mg.
• Akta Express has a Small footprint: A compact design allows the system to occupy minimal space on the bench or in the cold room.

ÄKTAxpress Module

• Complete Packsize: 1 Piece
• Application: Unattended multistep purification of tagged proteins and antibodies
• Flow Rate: 0.1-65 ml/min
• Operating Pressure Max.: 3 MPa (435 psi)
• Tubing i.d. [Flow Path]: 1 mm
• Single Wavelength Detection: Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436 and 546 nm.
• Multiple Wavelength Detection: No
• Flow Cell Path Length: 2 mm
• Multiple Sample Injection: Yes, up to 4 samples
• Autosampler Injection: No
• Automatic Buffer Preparation: No
• Buffer Selection: 10 buffer inlets
• Multi-Step Purification: Yes, up to 4 steps
• Relative Humidity: 20%-95% (non condensing)
• pH Stability Long Term: 2-12
• pH Stability Short Term: 1-14
• Viscosity: max 5 cP
• Width: 250 mm
• Height: 660 nm
• Depth: 600 mm
• Weight: 30 kg
• Voltage: 100-120/220-240 VAC
• Power Consumption: 120 VA
• Protection Class: IP 20
• Pump Type: Piston pump, metering type

Features:

• Frac-900 designed to work with AKTA series FPLC
• 95 places rack for 10-18mm tubes
• Fraction size 0.05 - 100 ml (0.001 ml increments)
• Time mode 0.01-9.999min, volume mode 0.01-9.999 ml
• Max flow rate 100 ml/min
• Peak detection and location capability, programmable collection modes

Features:

• Frac-900 designed to work with AKTA series FPLC
• 95 places rack for 10-18mm tubes
• Fraction size 0.05 - 100 ml (0.001 ml increments)
• Time mode 0.01-9.999min, volume mode 0.01-9.999 ml
• Max flow rate 100 ml/min
• Peak detection and location capability, programmable collection modes

Includes:

• Manual
• Vial Holder rack
• Power Cord

Specifications:

• Maximum flow rate: 100 ml/min
• pH stability range: 1 to 13, 1 to 14 (<1 day exposure)

Fraction size

• Volume mode: to 99999.99 ml
• Time mode: to 99999.99 min

No spillage range for DropSync

• Microplates: 0 to 1.0 ml/min
• 12 mm rack: 0 to 1.5 ml/min
• 18 and 30 mm rack: 0 to 2.0 ml/min

No spillage range for Accumulator

• All racks: 0 to 100 ml/min