Used Lab Equipment

Features include:
• Ability to store 3 separate user-programmed cycles
• RS 232 interface for printer or PC communication
• Delayed start and stop function
• Time range up to 99 hours and 59 minutes
• Digital temperature readout
• User-friendly interface
• 304 stainless steel chamber
• Working temperature from 5°C over ambient to 250°C (300°C as option)
• 5” of insulation for low heat radiation
• Natural low velocity air flow
• Easy open door latching system
• Attractive look and design
• Removable air distribution plates (no tools required)
• Two internal wire shelves included

Convincingly cost saving laboratory dryer with a 707-liter chamber volume. High-quality and safe drying, heating and tempering of common laboratory materials in laboratories and industry. High operation comfort, precise temperature regulation and short times of temperature recovery in the chamber after the door opening.

Specifications:

• Interior of stainless steel material: volume (22 l), depth (320 mm), width (240 mm), height (295 mm)
• External dimensions: depth (580 mm), diameter of the air branch outer/inner (52/49 mm), width (406 mm), height (604 (N) mm)
• Weight: 36 kg (gross)
• Heat emission at 250 °C: 300 W
• Air exchange speed at 250 °C: 6 per hour
• Working Temperature (from 5°C ambient temp): 250 °C

The Pharmacia Electrophoresis Power Supply EPS 500/400 is an accurately controlled power source of modern design that has been designed for safe and easy operation. The EPS 500/400 is designed to be used with the Pharmacia electrophoresis apparatuses (eg. the Gel Electrophoresis Apparatusus GE 2/4 and GE 2/4 LS, and the Flat Bed Electrophoresis apparatuses FBE Immuno and FBE 3000) or with any other suitable electrophoresis or immunoelectrophoresis chambers.

Description:

The Pharmacia Electrophoresis Power Supply EPS 3500 is a high quality, high precision and safe power supply for electrophoresis applications that require a maximum of 3500 volts. EPS 3500 is primarily designed for the following techniques: ● DNA sequencing ● IEF (Isoelectric focusing) EPS 3500 is also suitable for: ● ● ● ● ● SDS-PAGE (Polyacrylamide Gel Electrophoresis) Native PAGE Agarose electrophoresis Electroblotting DNA pulsed field electrophoresis Electrophoresis separations can be controlled by voltage, current or power. The EPS 3500 automatically switches over the controlling parameter according to programmed limits and conductivity variations in the system. Two electrophoresis units can be connected to the EPS 3500 and run with the same programmed method at one time. Three programs can be saved.

Specifications:

• Output modes: Constant voltage, current or power
• Output voltage: 1 - 600 V in 1 V steps
• Output current: 1 - 400 mA in 1 mA steps
• Output power: 1 - 100 W in 1 W steps
• Program storage: 3 programmed methods
• Time: 1 min – 500 hr
• Volthours: 1 Vhr - 500kVhr
• Power outlets: 2 pairs, recessed 4 mm high voltage
• Operating temperature: 4°-40°C
• Power Consumption: 140 W
• Mains power: 100-120/220-240 VAC, 50-60 Hz
• Dimensions (W x D x H ): 25 x 39 x 10 cm
• Weight: 4.8 kg
• Safety features: Overload/short circuit detection
• Automatic cross-over
• Automatic recovery after power failure

The BD FACSAria III sorter is built on the solid foundation of patented technologies, superior multicolor performance, and legendary ease-of-use that opened the complex world of cell sorting to a broader audience of researchers and wider range of applications. The fluidics and optical systems are precisely integrated to maximize signal detection. A patented flow cell with gel-coupled cuvette and patented octagon and trigon detection system allows the system to achieve unrivaled sensitivity and resolution.

Specifications:

• Laser Configuration: 405 nm (violet), 488 nm (blue/green), 633 nm (red) lasers
• Manufacture Date: August 2010
• Catalog No: 648282-03

Includes PM From BD and Guaranteed Installable under BD Service Contract

Upgraded to IIu and Added Violet laser in 2014

The new BD FACSAria II cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria II cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria II cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cell sorting.

Includes:

• 3 lasers
• Blue 7PMTs, Red 3PMTs, Violet 3 PMTs
• Computer w/ FACSDiva 8 Software
• ACDU
• 2 monitors
• Fluidics Cart

• Temperature Control
• Lauda Chiller

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

Includes PM From BD and Guaranteed Installable under BD Service Contract

New Flow Cell installed in September 2018

Also included are the excitation laser lines, laser powers and optical configuration.

The new BD FACSAria IIu cell sorter improves ease of use, flexibility, and aseptic capability to make it the best choice for consistent results in sorting across a broad range of applications. The heart of this cell sorting revolution is the true fixed-alignment flow cell, gel-coupled to the collection optics. Fixed alignment minimizes startup time and improves reproducibility. Gel coupling to the objective lens improves collection efficiency and increases the sensitivity and resolution needed for multicolor applications.

The BD FACSAria IIu cell sorter advances cell sorting with multiple improvements. A new fluidics design improves aseptic setup and cleaning. New nozzle sizes enable a wide range of particles to be sorted. Software improvements dramatically cut the time spent on cytometer setup, adjustment, and maintenance.

The BD FACSAria IIu cell sorter also sets a new standard for ease of use to improve both workflow and productivity. A new nozzle design allows for reproducible insertions. A new universal sort tube collection mount makes it easier to insert and remove tube holders. Software wizards and automation simplify aseptic sort setup, drop-delay determination, and clog detection. Compatibility with BD digital analyzers makes it easier to move from analysis to cellsorting.

Includes:

• 4 lasers (375nm, 488nm, 405nm, 633nm)
• Blue 7PMTs, Red 3PMTs, Violet 3PMTs
• HP Computer w/ FACSDiva 8.0 Software
• ACDU
• 1 HP monitor
• Fluidics Cart
• Temperature Control
• Lauda Chiller
• Buffalo Whisper Aerosol Control
• Under BD service contract since installed in 2012

Performance

Fluorescence Sensitivity

• Measurements performed at 70 psi and 90 kHz using SPHERO Rainbow Calibration Particles (RCP-30-5A)
• FITC: 125 molecules of equivalent soluble fluorochrome (MESF-FITC)
• PE: 125 molecules of equivalent soluble fluorochrome (MESF-PE)

Fluorescence Resolution

• Coefficient of variation (CV)
• PI: Area, <3.0%, full G0/G1 peak for propidium iodide (PI)-stained chicken erythrocyte nuclei (CEN)
• Hoechst: Area, <3.5%, full G0/G1 peak for Hoechst-stained CEN

Fluorescence Linearity

• Doublet/singlet ratio:
• CEN stained with PI: 1.95–2.05 (off the 488-nm laser) or Hoechst: 1.95–2.05 (off the 405-nm laser)

Forward and Side Scatter Sensitivity

• Sensitivity enables separation of fixed platelets from noise, identification of bacteria, and detection of 0.5-micron beads.

Forward and Side Scatter Resolution

• Scatter performance is optimized for resolving lymphocytes, monocytes, and granulocytes.

Sample Acquisition Rate

• Maximum acquisition rate (events per second) with 12 compensation pairs and 8 parameters: 70,000.

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software

• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• Laser
• 6 PMTs unit and side scatter

• All Necessary Cables
• Manuals

Optics Specifications:

• Lasers
  • Air-cooled:
  • 488-nm solid state, 20-mW laser output
  • 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 0 μm
• SSC Resolution: 5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP
• Fluorescence Detectors: 6 PMTs in 4-2 standard configuration
• Blue Laser Dyes
  • FITC, PE, PerCP or PerCP-Cy™5.5,
  • PE-Cy7 (525, 575, 678 or 695, 785 nm)
• Red Laser Dyes: APC, APC-Cy7 (660, 785 nm)
• Detector Bands
  • Blue Laser: 530/30; 585/42; >670; 780/60 nm
  • Red Laser: 660/20; 780/60 nm
• Fluorescence Threshold Sensitivities: FITC <100 MESF; PE <50 MESF

The BD FACSCanto system breaks new frontiers in flow cytometry by providing an efficient, easy-to-use yet powerful six-color benchtop analyzer. It incorporates many technology advances first developed in the BD LSR II expandable analyzer and the BD FACSAria cell sorter. It supports a broad range of research applications, being particularly suited for rare event analysis with its high speed, great sensitivity, and minimal sample-to-sample carryover.

The BD FACSCanto system combines a patented optical design for enhanced signal retention on six fluorescent and two scatter parameters, digital electronics for processing up to 10,000 events per second, and a novel sample injection tube supporting carryover of less than 0.1%. Your total benefit consists of the best fluorescence sensitivity to resolve dim events, cleaner data due to minimal sample contamination, and rapid processing rates, preserving valuable time for science. BD FACSCanto performance overcomes obstacles in rare event analysis and in functional studies for low detection ranges.

Includes:

• Computer w/ FacsDiva Software
• Fluids Cart
• 2 laser, Blue(488) and Red (633)
• 3 PMTs in 4-2 configuration w/ side scatter
• All Necessary Cables
• Manuals

Optics

Lasers

• Air-cooled
• 488-nm solid state, 20-mW laser output
• 633-nm HeNe, 17-mW laser output
• Laser Configuration: Spatially separated beams with 9 x 65-μm elliptical spots
• Optical Alignment Procedure: Fixed, no operator alignment required
• Flow Cell: 180 x 430-μm rectangular quartz flow cell
• Collection Optics: Optical-gel coupled 1.2 NA lens
• FSC Resolution: 1.0 μm
• SSC Resolution: 0.5 μm
• Fluorescence Detector Design: Reflective optics with single transmission filter in front of each PMT
• FSC Detector: Photodiode with 488/10 BP
• SSC Detector: PMT with 488/10 BP

• Fluorescence Detectors: 6 PMTs in 4-2 configuration w/ side scatter

Description:

• Compact, benchtop, constant voltage power supply designed for gel electrophoresis. Additionally suitable for Mini-SDS-PAGE and pulse field electrophoresis applications
• Equipped with dual banana output jacks and high-low voltage switch
• 3 digit digital readout, over-current alarm (500mA), check fuse indicator

Specifications:

• Weight: 12 lbs.
• Dimensions: 9” x 6” x 6”
• Power Supply Output: 20 to 250V in 10V increments, max 300 mA (at 150V – 250V), 500 mA (below 150V)

Description:

The E-Gel iBase Power System has an LCD display, which shows information about the program selected and running time. The display is located near the upper edge of the iBase device. Just below the display, the E-Gel iBase Power System has four buttons:

• Go button starts programs
• Mode button toggles between programs, minutes, and seconds
• Up button selects between programs on the display and increases running time
• Down button selects between programs on the display and decreases running time

An LED light (in the middle of the four buttons) indicates the status of the iBase device. The gel cassette is inserted into the two Electrode connections at the lower right of the iBase Power System. The back of the E-Gel iBase Power System contains a USB port and a power inlet. The supplied power cord has a matching connector that inserts into the power inlet, and connects the E-Gel® iBase™ Power System to the electrical outlet. A separate, stand-alone power supply is not required to run the iBase™ device.

Specification:

• Dimensions: 18.4 cm x 11 cm x 5.75 cm
• Weight: 500 g
• Electrical requirements: 100–240 V AC, 50/60 Hz< 1.0 A
• Temperature: 5°C to 40°C
• Built-in features: LCD Display, Alarm , LED light

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

Features:

The ImageQuant LAS 4000 is a camera system for producing digital images of gel or membrane samples or films. It is used together with the ImageQuant LAS 4000 Control Software to:

• Expose chemiluminescent samples or membranes.

• Expose fluorescent samples using epi (incident) or trans (transmitted) light sources, for example gels stained by ethidium bromide (EtBr). The ImageQuant LAS 4000 can be supplied with NIR, Red, Green, Blue and UV epi lights.

• Expose dye-stained gels and membranes or films using epi or trans illumination.

• Expose a sample repeatedly, incrementally or by programming exposure schemes.

Specifications:

• CCD: 3 200 000 pixels (Fujifilm super CCD
• Cooling temperature: -25°C
• Exposure time: 1/100 seconds to 2 hours (Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000 Control Software.)
• Lens: High-sensitivity lens F0.85 43 mm, Wide view lens F1.8 24 mm
• Number of gradations:

Recorded image: 16 bit

Focusing: 8 bit

The New Brunswick INNOVA 4000 Series are benchtop gyratory incubator shakers. Offering rigid control of temperature and agitation, the large-capacity INNOVA 4000 shaker is capable of culturing a wide array of organisms in test tubes and flasks up to 2.8 L.

The INNOVA series offers a wide temperature range. The INNOVA Model 4000 provides uniform temperature control in a range up to 60°C, while the high-temperature INNOVA 4080 incubator shaker increases the range to 80°C for added hybridization, adding capabilities for various molecular biology techniques, including hybridization studies, plasmid preps, DNA fingerprinting and more. The optional factory-installed cooling coil is also available for operating at sub-ambient conditions.

The INNOVA 4000 was designed with safety and ease of use in mind. The system includes an automatic cut-out feature of the shaker when the lid is opened to protect the operator. A fail-safe thermostat also turns off heat if temperature high limit is exceeded to protect cultures, and the hydraulic arm eases lid opening and closing.

Equipped with a large capacity blower and tightly constructed chamber, the INNOVA 4000 series incubator shaker has a self-correcting microprocessor feedback control which instantly compensates for changes in temperature. Another superior feature of the INNOVA 4000 include the interchangeable, large-capacity accessory platforms, 18" x 18" (46 x 46 cm) that accept gel trays, culture tubes and flasks to 2.8 L.

Specifications:

• Platform Size: 18 x 18
• Maximum Capacity (250 ml Flasks on Universal Platform): 18
• Capacity & Largest Flask Size on Universal Platform: 5 x 2L
• Orbit Diameter (in) 75
• Temperature Range (C°): Ambient +5 to 60 (4080 is Ambient +5 to 80)
• Digital Display: Yes
• Agitation: 25 to 500 rpm
• Type/Application: Benchtop
• Gassing Manifold: Optional
• Overall dimensions: W x D x H (in) 21 x 27.5 x 19.75
• Weight (lbs): 115

The New Brunswick INNOVA 4000 Series are benchtop gyratory incubator shakers. Offering rigid control of temperature and agitation, the large-capacity INNOVA 4000 shaker is capable of culturing a wide array of organisms in test tubes and flasks up to 2.8 L.

The INNOVA series offers a wide temperature range. The INNOVA Model 4000 provides uniform temperature control in a range up to 60°C, while the high-temperature INNOVA 4080 incubator shaker increases the range to 80°C for added hybridization, adding capabilities for various molecular biology techniques, including hybridization studies, plasmid preps, DNA fingerprinting and more. The optional factory-installed cooling coil is also available for operating at sub-ambient conditions.

The INNOVA 4000 was designed with safety and ease of use in mind. The system includes an automatic cut-out feature of the shaker when the lid is opened to protect the operator. A fail-safe thermostat also turns off heat if temperature high limit is exceeded to protect cultures, and the hydraulic arm eases lid opening and closing.

Equipped with a large capacity blower and tightly constructed chamber, the INNOVA 4000 series incubator shaker has a self-correcting microprocessor feedback control which instantly compensates for changes in temperature. Another superior feature of the INNOVA 4000 include the interchangeable, large-capacity accessory platforms, 18" x 18" (46 x 46 cm) that accept gel trays, culture tubes and flasks to 2.8 L.

Specifications:

• Platform Size: 18 x 18
• Maximum Capacity (250 ml Flasks on Universal Platform): 18
• Capacity & Largest Flask Size on Universal Platform: 5 x 2L
• Orbit Diameter (in)75
• Temperature Range (C°): Ambient +5 to 60 (4080 is Ambient +5 to 80)
• Digital Display: Yes
• Agitation: 25 to 500 rpm
• Type/Application: Benchtop
• Gassing Manifold: Optional
• Overall dimensions: W x D x H (in) 21 x 27.5 x 19.75
• Weight (lbs): 115