Used Lab Equipment

High-performance, fully automated imaging has made the AlphaImager HP our most popular platform. The system's application versatility not only includes a wide range of fluorescent and colorimetric applications, but can also be expanded to chemiluminescence as laboratory needs change.

Specifications:

• Camera Resolution: 4 megapixels
• A/D: 12-bit
• Lens: 8 – 48 mm, f/1.2 Zoom, motorized
• Maximum Field of View: 42 x 31 cm
• Filter Positions: 5, motorized
• Size: 15 x 20 x 24 in
• Weight: 80 lbs

Description:

High-content screening (HCS) or high-content analysis (HCA) requires an instrument designed to extract the maximum information from your sample in a robust and reproducible manner with the speed for high-throughput analysis. The entire Thermo Scientific portfolio of HCA instruments helps deliver on those parameters and includes:

• Superior illumination—the LED light engine reduces intensity fluctuations and optical component wear, eliminates moving parts, and reduces both integration and channel switching times
• Rapid data analysis—Thermo Scientific HCS Studio software analyzes your images in real time, so that you collect all the data you need to make decisions that count
• Reagent compatibility—Thermo Scientific HCS platforms build on the expertise of Molecular Probes in reagent development with exquisite fluorescent tools for interrogating the biology of the cell

Specifications:

• Camera: Photometrics X1 Camera with 4.54um/pixel
• Live cell: Optional HCA On-Stage Incubator with Kinetic scheduling of acquisition
• Software: HCA Studio 3.1 Cell Analysis Software. Optional client software available
• Database: STORE Express Image and Database Management Software Included, Scalable SQL/Oracle database options available

Features: 

• 20-MHz readout: High-speed, high-sensitivity image capture
• 2048 x 2048 imaging array: Resolves fine detail
• 7.4 x 7.4-ìm pixels: Ideally matched to optical microscope
• Interline-transfer, progressive-scan CCD: Eliminates camera vibration and facilitates fast triggering
• Flexible binning and readout: Increases light sensitivity while increasing the frame rate
• 12-bit digitization: Quantifies bright and dim signals in the same image
• Thermoelectric cooling: Long integration times for higher sensitivity
• Enhanced quantum efficiency: Provides higher sensitivity than typical interline cameras
• C-mount: Easily attaches to microscopes, standard lenses, or optical equipment
• Acquisition software: Captures, analyzes, and saves high-resolution images
• PCI interface: High-bandwidth, uninterrupted data transfer
• PVCam, Circular buffers, Device sequencing: Supported by numerous third-party software packages, Real-time focus, Precise integration with shutters, filter wheels, etc. (Compatible with Windows XP/Vista 32, Mac OS X, and Linux (kernel versions 2.4 and 2.6.8))
• CCD image sensor: Kodak KAI-4020M; interline-transfer, progressive-scan device with microlenses
• CCD format: 2048 x 2048 imaging array, 7.4 x 7.4-um pixels, 15.16 x 15.16-mm imaging area (optically centered)
• Linear full well: 30,000 e- (single pixel); 60,000 e- (2 x 2 binned pixel)
• Read noise: ≤10 e- rms @ 20 MHz
• Nonlinearity: <1%
• Digitizer type: 12 bits @ 20 MHz
• CCD temperature: -25ͦC (regulated)
• Dark current : 0.1 e-/p/s @ -25ͦC
• Operating environment: 0 to 30ͦC ambient, 0 to 80% relative humidity noncondensing
• I/O TTL (trigger/status): trigger, invert, inhibit, exposing, interline shift, frame readout, 8-bit TTL (programmable), 8-bit DACs(two)

Compare this against “Features of Gel Doc XRS+ system”: 

• Chemiluminescence: Yes
• Fluorescence: Yes
• Colorimetry/densitometry: Yes
• Gel documentation: Yes  

Hardware Specifications        

• Maximum sample size: 28 x 36 cm
• Maximum image area: 26X 35 cm
• Excitation source: Trans-UV and epi-white are standard (302 nm included, with 254 and 365 nm available as options)
• Illumination control: 3 modes (trans-UV, trans white, epi-white)
• Detector: super cooled CCD
• Image resolution: 4 megapixels
• Pixel size (H x V): 6.45 x 6.45 µm
• Filter holder: 3 positions (2 for filters, 1 without filter)
• Emission filters: 1 included (standard), 3 optional
• Dynamic range: >4.0 orders of magnitude
• Pixel density (gray levels): 65,535
• Dynamic flat fielding: Application specific, for all applications
• Instrument size (L x W x H): 36 x 60 x 96 cm
• Instrument weight: 32 kg

 Operating Ranges         

• Operating voltage: 110/115/230 V AC nominal
• Operating temperature: 10–28°C (21°C recommended)
• Operating humidity: <70% noncondensing

 Automation Capabilities            

• Workflow automated selection: Application driven; user selected or recalled by a protocol
• Workflow automated execution: Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
• Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
• Autofocus: Precalibrated focus for any zoom setting or sample height
• Image flat fielding: Dynamic; precalibrated and optimized per application
• Autoexposure: 2 user-defined modes (intense or faint bands)

Includes:

• Cabinet & Camera
• Computer With Software
• Manual

The Bio-Rad ChemiDoc XRS System is Bio-Rad’s most advanced imaging system. The system is able to image chemiluminescent, fluorescent and colorimetric samples and comes complete with a compact darkroom, transilluminator and supercooled high resolution 1.44 megapixel CCD camera. The entire system is controlled by the provided Quantity One 1-D analysis software with additional controls on the compact darkroom to control focus, aperture and zoom. Consequently, the ChemiDoc XRS system is extremely flexible and can be used for imaging a diverse range of applications such as Western blotting, DNA/RNA gel electrophoresis, 2-D gel electrophoresis, densitometry, etc. The system is able to detect HRP and AP chemiluminescence, ethidium bromide, SYBR Green, green fluorescent protein, silver stain and Coomasie brilliant blue staining to name but a few that are frequently used with the above applications. The system features flat fielding technology which allows for superior uniformity and quantification. Flat-fielding is a technique that corrects for pixel-to-pixel variations by dividing the gain variations out of an image, thus flattening the relative response for each pixel.

Operation of the system is very simple. The gel or membrane is placed on the transilluminator, which is set into a drawer which can be moved in and out of the darkroom, facilitating placement of the gel or membrane. Once the gel or membrane is placed, it and the transilluminator are returned to the darkroom and the door closed. With the Epi-illumination switched on, the aperture, zoom and focus are adjusted using the touchpad on the darkroom and viewed on the computer monitor until the desired position, size and focus of the gel are achieved. To acquire the image, the appropriate light source for the gel or membrane is selected and the software is used to select an integration time. Using the Quantity One software, the image is frozen when a satisfactory image is acquired. The Quantity One 1-D image analysis software aids the user in obtaining the best images possible from the gel or membrane. The software is user-friendly and equipped with built-in quick guides and wizards to facilitate the optimization of images and printouts. The software is compatible with both Microsoft Windows and Macintosh platforms. The Quantity One 1-D software is used for analysis of 1-D electrophoresis gels, Western blotting membranes, Coomassie-stained SDS-PAGE gels, etc. whereas the PDQuest 2-D analysis software is also available and is intended to be used for comparative analysis where many 2-D gels are analyzed at once.

Specifications:

• CCD Resolution (HxV): 1,392 x 1,040µm
• Pixel density: 65,536 gray levels
• Imaging area: 25 x 26 cm
• Camera cooling temperature: -50°C
• Camera cooling system: Peltier
• Dynamic range: 4 orders of magnitude
• Motorized zoom lens: C-mount, f/1.2, 12-75 mm
• Illumination modes: Epi-white, trans-UV (302 nm), and white

Using advanced electrically cooled SuperChemiNova CCD camera technology, the FluorChem provides real time display and the R.S.D.P. standard of resolution, sensitivity, dynamic range and performance. The FluorChem is ideal for chemiluminescence, fluorescence, and visible imaging applications.

Features:

• Applications: chemiluminescence, fluorescence and colorimetric
• Transillumination White: For protein gels,autorads,film, plates and flasks
• Transillumination UV: For the fluorescent gels such as EtBr, SYPRO Red, etc
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: High-performance, high resolution, CCD camera allows imaging of low-light samples in UV-illuminated, and fluorescent applications. 12.5x75mm, F1.2 Motorized Zoom Lens for ChemiImager 5500 with DE 500/400 cabinet (Includes motorized lens, ChemiImager 5500 camera mount, and cables. Requires AlphaEaseFC software version with software controls of motorized lens) -
• Lens: Zoom lens, close-up 2+ diopter lens and interference filter
• Filters: chemiluminescence samples, ethidium bromide, SYPRO Orange (595 nm) each filter has a bandwith +/-40 nm
• Allows to visualize chemiluminscent reactions

How is this unit better than Alpha Imager 3400:

For low-light applications, such as chemiluminescence, a longer exposure time may be appropriate and it is best to go to the red ACQUIRE IMAGE button to directly acquire the image once the desired exposure time is set. Alternatively, if a good exposure time is unknown, try selecting Auto-Expose and using the super sensitivity selection. Auto-Expose will generate an image showing the signal intensity usually in less than 3 minutes. Afterwards, a different sensitivity (binning) mode can be selected to generate a better resolution image and the software will automatically calculate the new exposure time for you.

 Features:

• Applications: fluorescence and colorimetric
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: high sensitivity, high resolution scientific grade CCD camera with 2.0 megapixel resolution, extendable to 6.0 megapixels
• Lens:5-75 f/1.2
• Overhead Light Selector: White light, 365 nm UV, 254 nm UV
• Flters: ethidium bromide, SYBR Green, SYBR Gold
• Visible color stains: Coomassie, Silver
• Allows to visualize chemiluminscent reactions
• Manual lift platform, 3 emission filters, white light plate, overhead UV, white light and blue light
• Transilluminator: Choice of UV transilluminator models

Using advanced electrically cooled SuperChemiNova CCD camera technology, the FluorChem provides real time display and the R.S.D.P. standard of resolution, sensitivity, dynamic range and performance. The FluorChem is ideal for chemiluminescence, fluorescence, and visible imaging applications.

Features:

• Applications: chemiluminescence, fluorescence and colorimetric
• Transillumination White: For protein gels,autorads,film, plates and flasks
• Transillumination UV: For the fluorescent gels such as EtBr, SYPRO Red, etc
• Software: easy-to-use software for darkroom control, image capture, enhancement and analysis
• Camera: High-performance, high resolution, CCD camera allows imaging of low-light samples in UV-illuminated, and fluorescent applications. 12.5x75mm, F1.2 Motorized Zoom Lens for ChemiImager 5500 with DE 500/400 cabinet (Includes motorized lens, ChemiImager 5500 camera mount, and cables. Requires AlphaEaseFC software version with software controls of motorized lens) -
• Lens: Zoom lens, close-up 2+ diopter lens and interference filter
• Filters: chemiluminescence samples, ethidium bromide, SYPRO Orange (595 nm) each filter has a bandwith +/-40 nm
• Allows to visualize chemiluminscent reactions

How is this unit better than Alpha Imager 3400:

For low-light applications, such as chemiluminescence, a longer exposure time may be appropriate and it is best to go to the red ACQUIRE IMAGE button to directly acquire the image once the desired exposure time is set. Alternatively, if a good exposure time is unknown, try selecting Auto-Expose and using the super sensitivity selection. Auto-Expose will generate an image showing the signal intensity usually in less than 3 minutes. Afterwards, a different sensitivity (binning) mode can be selected to generate a better resolution image and the software will automatically calculate the new exposure time for you.

Features:

• Transillumination White: For protein gels, autorads, film, plates, flasks
• Transillumination UV: For fluorescent gels such EtBr, SYPRO Red, SYBR Safe, etc.
• Reflective White: For colorimetric blots and membranes
• For chemiluminescence
• High-performance, high resolution, CCD camera
• Manual f/2.8 28-70mm zoom lens
• 92 Megapixel Resolution
• Advanced Camera with 16-bit 1MHz Digitization
• Regulated Cooling and Low Noise Readout
• Multiple Image Acquisition Modes for a Wide Range of Applications

Includes Computer Loaded with Software

The FluorChem HD2 System provides a complete array of image acquisition modes for common fluorescent and chemiluminescent applications.  The FluorChem HD2 System delivers higher sensitivity and a broader dynamic range than film can provide at similar exposure times. Captured images are saved digitally, making them easy to import into presentations and reports

Features:

• Applications Types: Chemiluminescent, Colorimetric, uv, Fluorescent
• Camera Resolution: 19 megapixels (2048 x 2048)
• a/D: 16-bit
• Recommended lens: 50 mm, f/0.95 Fixed, manual or motorized
• Pixels: 4 x 7.4 μm
• Filter Positions: 5, motorized
• Size: 20" W x 15" D x 39.5" h (51 x 38 x 100 cm)
• Weight: 100 lbs (45.4 kg)

Description:

• Programmable pre-analysis sample preparation
• Heating & cooling features
• Self-contained system
• Vertical set-up saves bench space
• Standard SBS format labware
• Disposable pipette tips
• High accuracy fluid dispensing
• Complete control of workflows
• Particle identification via Microsoft Excel®

Specifications:

• Operating principle: Single robotic XYZ arm, hydraulic fill on all fluidics
• Labware format: SBS
• Number of positions for labware: 7
• Maximum number of wells per deck position: 96
• Maximum volume dispensed per operation: 1000μl
• Minimum volume dispensed during dilution or reagent operations: 5μl
• Minimum volume dispensed into FlowCam for analysis: 100μl
• CV of aspiration/dispensing accuracy: ±0.5%
• Pipette format: Disposable
• Environmental (particulate) control: Positive pressure HEPA filtration
• Sterilization: UV, programmable exposure time
• Mechanical orbital method: 100 -2000 RPM clockwise
• Sample conditioning: Heating/cooling 2-90°C
• Mixing: Aspiration/dispensing method
• Multiple assays/workflows supported: Yes
• Operation pause and resume: Yes
• CFR21 Compliant: Yes
• Workspace illumination: User controlled white LED
• Dimensions (H x W x D): 49 x 30 x 25” (124.5 x 76.2 x 63.5 cm)
• Weight: 190 lbs. (86.7 kg)
• Voltage: 110-220 V
• Power: 700 watts, including FlowCam
• Operating Temperature Operating: 5-40°C
• Storage Temperature: -20-60°C

Includes:

• Cooling Module
• Control Module
• Drive Module

Drive Module 

• Dimensions (W x D x H): 43 x 26 x 14 cm
• Construction: Aluminum chassis
• Weight: 8 kg (17.2 lb)
• Maximum voltage:250 V DC
• Maximum current: 500 mA
• Electrode potentials:Dynamically regulated (feedback adjustment) +/- 0.5%
• Input voltage range:
  • 90–132 VAC/47–63 Hz/0.7 A (maximum)
  • 198–264 VAC/47–63 Hz/0.35 A (maximum)
• Fuses:
  • 5 A Fast Blow for high voltage output (front panel)
  • A Slow-Blow (100/120 V) or
  • 5 A Slow-Blow (220/240 V)

Control Module          

• Dimensions (W x D x H): 31 x 13.5 x 17 cm
• Maximum current: 500 mA
• Allowable voltage gradients:6–6 V/cm, in 0.1 V/cm increments
• Data entry: Keyboard
• Power supply: 250 V maximum, to allow maximum gradient of 6 V/cm, continuously adjustable

Cooling Module

• Chassis: Aluminum
• Dimensions (W x L x H):23 x 42 x 24 cm
• Weight: 14 kg (30.1 lb)
• Compressor:1/12 HP
• Refrigerant:CFC 12
• Heat exchanger:Stainless steel
• Operating range: 5°C–25°C
• Cooling rate (pre-cool):75°C/min from ambient to 14°C
• Cooling capacity (input power): 75 W at 14°C
• Input voltage range       
  • 90–132 VAC/47–63 Hz/4 A
  • 198–264 VAC/47–63 Hz/2 A
• Fuses   
• 3 A Slow-Blow (100/120 V) or
• 0 A Slow-Blow (220/240 V)

The Mini Gel Tank is compatible with a variety of Novex mini gels, NuPAGE mini gels, and Bolt Bis-Tris Plus gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient side-by-side gel loading and enhanced viewing during use. Optimized conditions using constant voltage allow for Bolt Bis-Tris Plus gels to be run in approximately 35 minutes. Run times may vary depending on buffer conditions and power supplies being used.

Specifcations:

• Capacity: Up to 2 gels per run
• Gel Compatibility: Bolt Bis-Tris Plus Gels, Novex Mini Gels