Used Lab Equipment

Description:

The HiSeq 2500 system is a high throughput sequencing system that combines scalable output with flexible (dual) run modes which supports multiple applications, and superior turnaround times. With rapid or high-output modes and single or dual flow cell options, the HiSeq 2500 can be tuned to meet output needs ranging from 9 Gb to 1 Tb or 300 million to 4 billion reads per run. This system is a 2014 model that has recently been deinstalled by Illumina and includes a cBot. It can run the V3 high output chemistry as-is, or V4 with a hardware upgrade from Illumina.  This can also run the most recent version of the rapid run chemistry.

Specifications:

• Dimensions - W x D x H: 118.6 cm x 76.0 cm x 94.0 cm (46.7 in x 30.0 in x 37.0 in)
• Weight: 221.4 kg (488 lbs)
• Dual mode processing (High Output and Rapid Run)
• HiSeq Control Software
• Instrument Control Computer                       
• Base Unit: Intel Xeon CPU E5-2620 @ 2.00 GHz (2 processors)
• Memory: 64 GB RAM
• Hard Drive: 8 x 1.0 TB 3.5” Enterprise SATA
• Operating System: Microsoft Windows 7
• Power Requirements: 100 - 240V AC 50/60Hz, 20A, 1500 W
• Instrument Configuration: Computer and touch screen display Installation setup and accessories Data collection and analysis software
• Laser: 532 nm, 660 nm, 650 nm

Includes:

• Applied Biosystems 3730XL
• 96-Capillary
• Computer w/ Pre-loaded existing software
• Data Collections 3.0
• Warranty Period: 90 days
• Installation on site

Specifications 

• Laser: Argon-ion multi line, single mode laser: primary excitation lines 488 and 515.5nm
• Oven Temp: 18°C to 70°C
• Sample Volumes: 384 well plates = 5-30µl / 96 well plates = 10-50µl
• Plate Seal: Septa or Polypropylene heat seal
• Operating Environment: 18°C - 30°C, Humidity = 20 to 80%
• Capillary Array Lengths: 36 cm or 50 cm
• Capillary Dimensions: 150 5m o.d. / 50 5m i.d.
• Polymer Consumption
  • 36cm: approx. 200µl per run
  • 50cm: approx. 250µl per run

ABI 3730xl DNA Sequencer         

• Optics
  • In-capillary detection by dual-side illumination.
  • Multiple fiter sets: Z, E, G5, G5-RCT, Any4Dyeset, Any5Dyeset and S (optional)
  • Backside thinned CCD for maximum optical sensitivity
• Capillaries
  • Choice of capillary lengths, 36cm or 50cm
  • Dimensions: 150µm o.d./50µm i.d.
• Reagent Handling
  • Automated polymer replenishment pump for increase throughput
  • POP-7 performance robust polymer separation matrix
  • 5 sequencing run modules, 1 fragment analysis run and SNPlex run module
  • On-board reagents for up to 48-hours of unattended operation
• Performance Per Run Type  
  • LongSeq: 1152 samples per day (980 Kbases)
  • StdSeq: 2304 samples per day (1550 Kbases)
  • FastSeq: 2304 samples per day (1600 Kbases)
  • RapidSeq: 3840 samples per day (2100 Kbases)
  • GeneMapper Software
    • 4224 samples per day (92,000 genotypes)
    • 36 cm, up to 500 bp resolution with 0.15 bp sizing precision in 34 minutes

Power Requirements       

• Voltage: 200-220 volt or 230-240 volt

System Dimensions        

• Width 100 cm
• Depth 73 cm
• Height 89 cm
• Weight 180 kg

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit