Used Lab Equipment

Description:

The HiSeq 2500 system is a high throughput sequencing system that combines scalable output with flexible (dual) run modes which supports multiple applications, and superior turnaround times. With rapid or high-output modes and single or dual flow cell options, the HiSeq 2500 can be tuned to meet output needs ranging from 9 Gb to 1 Tb or 300 million to 4 billion reads per run. This system is a 2014 model that has recently been deinstalled by Illumina and includes a cBot. It can run the V3 high output chemistry as-is, or V4 with a hardware upgrade from Illumina.  This can also run the most recent version of the rapid run chemistry.

Specifications:

• Dimensions - W x D x H: 118.6 cm x 76.0 cm x 94.0 cm (46.7 in x 30.0 in x 37.0 in)
• Weight: 221.4 kg (488 lbs)
• Dual mode processing (High Output and Rapid Run)
• HiSeq Control Software
• Instrument Control Computer                       
• Base Unit: Intel Xeon CPU E5-2620 @ 2.00 GHz (2 processors)
• Memory: 64 GB RAM
• Hard Drive: 8 x 1.0 TB 3.5” Enterprise SATA
• Operating System: Microsoft Windows 7
• Power Requirements: 100 - 240V AC 50/60Hz, 20A, 1500 W
• Instrument Configuration: Computer and touch screen display Installation setup and accessories Data collection and analysis software
• Laser: 532 nm, 660 nm, 650 nm

Includes:

• Applied Biosystems 3730XL
• 96-Capillary
• Computer w/ Pre-loaded existing software
• Data Collections 3.0
• Warranty Period: 90 days
• Installation on site

Specifications 

• Laser: Argon-ion multi line, single mode laser: primary excitation lines 488 and 515.5nm
• Oven Temp: 18°C to 70°C
• Sample Volumes: 384 well plates = 5-30µl / 96 well plates = 10-50µl
• Plate Seal: Septa or Polypropylene heat seal
• Operating Environment: 18°C - 30°C, Humidity = 20 to 80%
• Capillary Array Lengths: 36 cm or 50 cm
• Capillary Dimensions: 150 5m o.d. / 50 5m i.d.
• Polymer Consumption
  • 36cm: approx. 200µl per run
  • 50cm: approx. 250µl per run

ABI 3730xl DNA Sequencer         

• Optics
  • In-capillary detection by dual-side illumination.
  • Multiple fiter sets: Z, E, G5, G5-RCT, Any4Dyeset, Any5Dyeset and S (optional)
  • Backside thinned CCD for maximum optical sensitivity
• Capillaries
  • Choice of capillary lengths, 36cm or 50cm
  • Dimensions: 150µm o.d./50µm i.d.
• Reagent Handling
  • Automated polymer replenishment pump for increase throughput
  • POP-7 performance robust polymer separation matrix
  • 5 sequencing run modules, 1 fragment analysis run and SNPlex run module
  • On-board reagents for up to 48-hours of unattended operation
• Performance Per Run Type  
  • LongSeq: 1152 samples per day (980 Kbases)
  • StdSeq: 2304 samples per day (1550 Kbases)
  • FastSeq: 2304 samples per day (1600 Kbases)
  • RapidSeq: 3840 samples per day (2100 Kbases)
  • GeneMapper Software
    • 4224 samples per day (92,000 genotypes)
    • 36 cm, up to 500 bp resolution with 0.15 bp sizing precision in 34 minutes

Power Requirements       

• Voltage: 200-220 volt or 230-240 volt

System Dimensions        

• Width 100 cm
• Depth 73 cm
• Height 89 cm
• Weight 180 kg

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

The DNA Engine Tetrad 2 thermal cycler is a four-bay thermal cycler ideal for high-throughput research and production environments. The Tetrad 2 thermal cycler is an updated version of the cycler which powered the Human Genome Project.

Features:

• High-throughput cycling in a compact footprint
• Intuitive graphical interface for rapid input of protocols and easy file management
• Interchangeable Alpha units with adjustable heated lids that accommodate a wide range of reaction vessels and sealing options
• Excellent temperature accuracy and uniformity for consistent results
• Real-time displays of block and lid temperatures provide quality assurance that the thermal cycler is performing as directed
• Software features, such as error tracking logs, run tracking files, and file management tools, offer valuable information for high-throughput runs

Alpha unit modules are available in several formats, including:

• Gradient-enabled 96-well unit that allows simultaneous incubation at 8 different temperatures to optimize reactions in a single run
• Dual 48/48-well unit that allows 2 independent experiments to run simultaneously
• High-throughput 384-well unit

Includes:

• Computer w/ GenePix Pro -  MicroArray Acquisition & Analysis Software
• Flat Panel Monitor
• All Necessary Cords, Cables
• Manual

Features:

• Speed. Unlike most commercially available microarray scanners, the GenePix 4000B Microarray Scanner acquires data at two wavelengths simultaneously. This means drastically reduced scan times and hence greater workflow efficiency. Apart from superior speed, simultaneous scanning eliminates potential errors associated with aligning two separately scanned images, and provides real-time access to data as they are acquired.
• Add Reliability to Your Research. The performance calibration standards that come with your GenePix 4000B Microarray Scanner allow you to check the performance when you receive your scanner and at any time in the future. GenePix Pro Acquisition and Analysis Software includes one-touch calibration to restore any GenePix scanner to benchmark performance. You can rest assured knowing the signals you generate today are the same as a couple of years ago and in the future.
• Automate Your Workflow. With automated photomultiplier (PMT) balancing. The fully integrated GenePix Pro Acquisition and Analysis Software, controls the GenePix 4000B Microarray Scanner and can automatically evaluate the required PMT gain values, for fast and easy optimization of signal intensity and channel balance. The GenePix 4000B Microarray Scanner then acquires data at your selected resolution between 5-100 micron, allowing the adjustment of image resolution and file size for each experiment.
• Get More Published. For over 10 years and close to 6000 literature references, the GenePix 4000B Microarray Scanner has expanded the overall sample compatibility, with user-adjustable focus offset and laser power settings. This allows proper imaging of slides with either a raised surface, as with membrane-coated glass, or a recessed surface, as in embedded arrays.

Performance Specifications

• Sample type: Standard microscope slide (1" x 3", 25 x 75 mm or 26 x 76 mm)
• Scan area: Adjustable, 22 x 71.5 mm max.
• Excitation: 532 nm and 635 nm solid-state lasers
• Laser power settings: User-selectable 100%, 33% or 10% laser power
• Emission filters: Two, optimized for Cy3 and Cy5 or spectrally similar dyes
• Detection: Dual photomultipliers (PMTs), automatic and manual gain adjustment
• Focus offset: Adjustable between -50 and +200 μm (5 μm increments)
• Optics: Non-confocal
• Scanning method: Simultaneous, 2-channel
• Scan time:5 minutes for both channels, 10 μm resolution, full scan area
• Pixel resolution: Adjustable from 5-100 μm
• Digital resolution: 16-bit
• Dynamic range: Four orders of magnitude at SNR > 3
• Image type: Single- or multi-image TIFF
• Barcode reading: Image-based software barcode reader

General Specifications

• Dimensions (in.):5 (W) x 8.0 (H) x 17.5 (D)
• Dimensions (cm): 34 (W) x 20 (H) x 44 (D)
• Weight: 45 lbs. (20 kg)
• Power supply: 110/220V universal
• Genomics: Directed at the genome sequence itself, microarrays have been used to identify novel genes, binding sites of transcription factors, changes in DNA copy number, and variations from a baseline sequence, such as in emerging strains of pathogens or complex mutations in disease-causing human genes.