Used Lab Equipment

Specifications:

• Hydraulic system: Two dual piston in series, with proprietary servo-controlled variable stroke drive, floating piston, active inlet valve, solvent selection valve and electronic flow control for flow rates up to 100 µl/min
• Settable column flow Range:
  • – 20 µl/min
  • – 100 µl/min (with the extended flow range kit)
  • – 2.5 µl/min (with the electronic flow control bypassed)
• Recommended column flow range:
  • – 20 µl/min
  • 10 – 100 µl/min (with extended flow range kit)
  • – 2.5 ml/min (with the electronic flow sensor bypassed)
• Column flow precision: < 0.7 % RSD or 0.03 % SD (typically 0.4 % RSD or 0.02 % SD), at 10 µl/min and 50 µl/min column flow (based on RT, default setting)
• Optimum composition Range: 1 to 99% or 5 µl/min per channel (primary flow), whatever is greater
• Composition precision: < 0.2 % SD, at 10 µl/min (20 µl flow sensor), 50 µl/min (100 µl flow sensor) and 1 ml/min (normal mode) default setting
• Delay volume:
  • Typically 3 µl from the electronic flow control to the pump outlet for flow rates up to 20 µl/min.
  • Typically 12 µl from the electronic flow control to the pump outlet for flow rates up to 100 µl.
  • for flow rates up to 100 µl/min and electronic flow control active:
  • primary flow path 180 - 480 µl without mixer, 600 - 900 µl with mixer (system pressure dependent)
  • Typically 180 to 480 µl (system pressure dependent) without mixer for flow rates up to 2.5 ml/min. (Mixer delay volume 420 µl)
• Pressure range: 20 to 400 bar (5880psi) system pressure
• Compressibility Compensation: User-selectable, based on mobile phase compressibility
• Recommended pH Range: 0 – 8.5, solvents with pH < 2.3 should not contain acids which attack stainless steel. Upper pH range is limited by fused silica capillaries.
• Control and data Evaluation: Agilent ChemStation for LC
• Analog output For pressure monitoring, 2 mV/bar, one output
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user-settable limits and feedback messages. Electronic records of maintenance and errors.
• Housing: All materials recyclable

The Agilent 1200 Series autosampler is designed for use with other modules of the Agilent 1200 Series LC system or with other LC systems if adequate remote control inputs and outputs are available. The autosampler is controlled from the Agilent 1200 Series control module or from the Agilent ChemStation

for LC systems.

Specifications:

• Weight: 15.5 kg (34.2 lbs)
• Dimensions (height × width × depth): 200 × 345 × 440 mm (8 × 13.5 × 17 inches)
• Line voltage: 100 – 240 VAC, ±10 % Wide-ranging capability
• Line frequency: 50 or 60 Hz, ±5 %
• Power consumption (apparent power): 300 VA Maximum
• Power consumption (active power): 200 W Maximum
• Ambient operating temperature: 4 to 55 8C (41 to 131 8F)
• Ambient non-operating temperature: -40 to 70 8C (-4 to 158 8F)
• Humidity: < 95 %, at 25 to 40 8C (77 to 104 8F) Non-condensing
• Operating Altitude: Up to 2000 m (6500 ft)
• Non-operating altitude: Up to 4600 m (14950 ft) For storing the autosampler

Specifications:

• Flow rate range: 1 to 5000 μL/min
• Backpressure range 0 to 600 bar (8702 psi)
• Residual pulsation < 2 bar Amplitude
• Flow rate accuracy ±0.5% or 1 μL/min, whichever is greater
• Gradient composition accuracy ±0.5% absolute
• Gradient resolution 1% from 0 to 100%
• Firmware version (November 2010) 23.67 e
• Gradient composition range 0 to 100% from up to 4 independent feed channels employing 2 of them at the same time
• Delay volume 90 μL (LDA) + 65 μL (static mixer)

The HP / Agilent 1100 HPLC series is the current model and arguably the best design in the industry because of the optimized design of the quaternary pump ensuring virtually pulse-free and stable solvent flow, with dual floating pistons in series, precisely servo-controlled. This design with variable stroke volume allows pulse-free solvent delivery and efficient mixing. An inlet-valve electronically activated and synchronized to piston stroke - eliminates vapor formation with volatile solvents and is maintenance free. With high-speed proportioning valve and pre-configured vacuum degasser, for convenient low-pressure mixing of up to four solvents and highest flexibility in solvent choices.

 Features:

• Ease-of-use - handheld control module lets you enter operating parameters fast, store methods easily and display pressure profile, diagnostics and task-oriented information.
• Durable - stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE ensure long lifetime. Seal wash option for high salt mobile phases keeps maintenance to a minimum for lower operation costs.
• Wide flow range - up to 10 ml/min for broad HPLC work, including semi-preparative separations.
• Better for GLP - built-in leak and pressure test with graphical presentation of pressure profiles, and early maintenance feedback.

Specifications:

• Hydraulic system: Dual piston in series pump with proprietary servo-controlled variable stroke drive, floating pistons and active inlet valve
• Settable flow range: .001– 10 ml/min, in 0.001 ml/min increments
• Flow range: 2 – 10.0 ml/min
• Recommended pH range: – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel.
• Operating voltage: 230V

The G1311A is an optimized design ensuring virtually pulse-free and stable solvent flow with dual floating pistions.  An inlet valve electronically activated and synchronized to piston stroke eliminates vapor formation with volatile solvents and is maintenance free.  Instrument comes equipped with four solvent inlets with built-in gradient control. 

Specifications:

• Configurable Flow Range: 0.001 – 10ml/min – in 0.001ml/min increments
• Flow Range: 0.2 – 10.0 ml/min
• Recommended pH Range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
• Gradient Formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve. Delay volume 800-1100µl, depending on back pressure

The G1311A is an optimized design ensuring virtually pulse-free and stable solvent flow with dual floating pistions.  An inlet valve electronically activated and synchronized to piston stroke eliminates vapor formation with volatile solvents and is maintenance free.  Instrument comes equipped with four solvent inlets with built-in gradient control. 

Specifications:

• Configurable Flow Range: 0.001 – 10ml/min – in 0.001ml/min increments
• Flow Range: 0.2 – 10.0 ml/min
• Recommended pH Range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
• Gradient Formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve. Delay volume 800-1100µl, depending on back pressure

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack

Features:

• Extended flow rate range
• Without changing pump heads, provides flow rates up to 100 mL/min at 400 bar
• For preparative and analytical applications with low flow rates (0.5 mL/min, upward), provides precise high-pressure binary gradient formation with low delay volume (700 uL)
• Automated seal wash using a peristaltic pump extends seal lifetime
• Pumps stop in response to column blockage or leaks
• Automated electromagnetic purge valve simplifies system setup
• Software provides solvent level control that keeps the pump from running dry

Can be sold as an individual component or as part of a complete stack

The G1311A is an optimized design ensuring virtually pulse-free and stable solvent flow with dual floating pistions.  An inlet valve electronically activated and synchronized to piston stroke eliminates vapor formation with volatile solvents and is maintenance free.  Instrument comes equipped with four solvent inlets with built-in gradient control. 

Specifications:

• Configurable Flow Range: 0.001 – 10ml/min – in 0.001ml/min increments
• Flow Range: 0.2 – 10.0 ml/min
• Recommended pH Range: 1.0 – 12.5, solvents with pH > 2.3 should not contain acids which attack stainless steel
• Gradient Formation: Low pressure quaternary mixing/gradient capability using proprietary high-speed proportioning valve. Delay volume 800-1100µl, depending on back pressure

This Agilent 1200 Model G2226A ( G 2226A, G 2226 A, G2226 A ) Series Nano (NanoFlow) Pump is in excellent condition!  The Agilent 1200 Series nanoflow pump uses a unique solvent delivery technology optimized for nanoliter-per-minute flows and features EFC (Electronic Flow Control) with active feedback and real time flow adjustment. EFC is the gold standard for robust and reliable nanoflow performance and delivers the precise flow and gradient performance necessary for retention time reproducibility and stable ion generation essential for high sensitivity nanospray MS performance.

Features:

• Electronic Flow Control (EFC) provides real-time adjustment of the flow rate throughout the analysis for reliable and reproducible results.
• Micro vacuum degassing with very low internal volume per channel (1 mL) for fast changeover of mobile phase and quick purge.
• Control from handheld controller for convenient local status monitoring.
• 2nd generation flow sensors.
• Application focus: High resolution separations and high sensitivity MS detection. Proteomics is a typical application area for the nanoflow pump.

Features:

This versatile and highly reliable pump offers flow rates up to 10 mL/min and pressures up to 600 bar. It is suited for the use of smaller particle size columns giving higher resolution and faster separations. It maintains virtually pulse-free, well mixed and stable solvent flows. Its dual floating, precise servo-controlled pistons adjust the stroke volume according to your chosen flow rate. Offering access to up to four solvents, the 1260 Infinity II Quaternary Pump provides the greatest flexibility in automated solvent blending. It is recommended for a wide range of research and routine applications (especially in the food, environmental and pharmaceutical sectors) as well as for method development.

Specifications:

• Higher resolution, faster separations - the maximum pressure of 600 bar allows you to use smaller particle size columns, longer columns and/or higher viscous solvents as an alternative to acetonitrile.
• Analytical and semi-preparative purifications - the wide flow range (up to 10 mL/min) perfectly supports standard and semi-preparative applications. The perfect choice for 4.6 and 3.0 mm id columns.
• Quicker experiments - the pump offers convenient access to up to four solvents for isocratic or gradient analysis, which can really speed up method development, preparation of mobile phases and system flushing. 
• Easy, fast and secure transfer of methods - from conventional to sub-2-micron columns.
• Integrated degasser
• Longevity and low operating costs - robust materials (stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE) withstand the most demanding applications and keep your pump functioning for years. Our optional seal wash for high-salt mobile phases keeps maintenance to a minimum and reduces operating costs.

The Agilent Technologies 1200 Series (G1310B) 1260 Infinity Isocratic Pump is a HPLC workhorse for demanding QA/QC and routine applications, designed to provide virtually pulse-free and stable solvent flow. This delivery principle allows variable stroke volume optimized to selected flow rate. The broadened pressure range with maximum pressure of 600 bar allows the use of small particle size columns, longer columns, or alternative solvents of higher viscosity. Additionally, the G1310B is the ideal pump for GPC/SEC applications where run-to-run and day-to-day precision of retention times are important.

Specifications:

• Settable flow range: set points 0.001 - 5 mL/min, in 0.001 mL/min increments
• Flow range: 0.2 to 10.0 mL/min
• Flow precision: ≤ 0.07 % RSD or ≤ 0.02 min SD
• Flow accuracy: ± 1 % or 10 μL/min whatever is greater, pumping degassed H2O at 10 MPa (100 bar)
• Pressure operating range:
  • Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min
  • Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min

1260 Infinity - G1311B 

The quaternary pump comprises an optional solvent cabinet, a vacuum degasser

 and a four- channel gradient pump. The four- channel gradient 

pump comprises a high- speed proportioning valve and a pump assembly. 

It provides gradient generation by low pressure mixing. A solvent cabinet 

provides enough space for four one- liter bottles. An active seal wash 

(optional) is available when the quaternary pump is used with 

concentrated buffer solution 

Specifications:  

• Hydraulic System: Dual piston in series pump with servo-controlled variable stroke drive, 
• power transmission by gears and ball screws, floating pistons 
• Setable Flow Range: Set points 0.001 – 10 mL/min, in 0.001 mL/min increments 
• Flow Range: 0.2 – 10.0 mL/min 
• Flow Precision: ≤ 0.07 % RSD, or ≤ 0.02 min SD whatever is greater, based on retention time at constant room temperature 
• Flow Accuracy: ±1 % or 10 µL/min whatever is greater, pumping degassed H2O at 10 MPa (100 bar) 
• Pressure Operating Range: Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min, Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min 
• Pressure Pulsation: < 2 % amplitude (typically < 1.3 %), or < 0.3 MPa (3 bar), whatever is greater, at 1 mL/min isopropanol, at all pressures > 1 MPa (10 bar,147 psi) 

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack