Used Lab Equipment

The RF10AXL, Shimadzu's newest fluorescence detector for HPLC, offers a refined optical design for the ultimate in high sensitivity detection. This unit offers an improved Raman signal to noise ratio of 300 for excellent sensitivity, improved wavelength accuracy of ± 2.0 nm for strong betweenunit consistency. A newly developed ratio system compensates for lamp intensity fluctuation and provides superb noise characteristics. The RF10AXL features other enhancements including a new remote lamp on/off function to allow lamp shutdown upon run completion, and a new rugged flow cell design with elevated back pressure tolerance and backward compatibility with the RF10A.  The RF10AXL has a compact, modular design and is used with an SCL10Avp system controller to complement any existing HPLC system. It easily integrates with other Shimadzu LC10A Series components via fiber optic communication cables for "plug and play" convenience in building a customized Shimadzu LC system. The RF10AXL offers many buil in GLP functions including self diagnostics, maintenance information (lamp lit hours, lamp energy), and span calibration functionality.

Specifications

• Light source: High Precision Xenon lamp, 150W
• Excitation/emission monochromators: Concave, blazed holographic grating monochromators,F2.4
• Measuring wavelength range: 200-650 nm (200-900nm option)
• Spectral bandwidth: 15 nm both in the excitation and emission sides
• Wavelength accuracy: +/- 2 nm
• Wavelength reproducibility: +/- 0.2 nm
• Sensitivity: The S/N ratio is 300 for the Raman line of distilled water(350nm excitation wavelength and 1.5 sec. time constant)
• Detector: Photomultiplier
• Cell (volume, pressure, material): 12 =L, approx. 20kgf/cm2 (2MPa) SUS316, Quartz, PTFE, as standard (2 =L cell and inert cells are available as option)
• Wavelength scanning:Possible both for excitation and emission wavelengths.
• Time programming: Wavelengths parameters may be programmed in up to 32 steps
• Dimensions, weight: 260W x 520D x 205H mm, 17kg
• Ambient temperature requirements: 4 - 35° C
• Power requirements: 100-240 V as ordered, 350 VA, 50/60 Hz

This user-friendly detector brings high sensitivity fluorescence detection for you needs whether used alone or in an automated Agilent 1200 Series system. One run will provide quantitative data and fluorescence spectra all the while improving sensitivity and selectivity with the simultaneous multi-wavelength detection capabilities.

Specifications:

• Environment: 0 – 40°C
• Dimensions: 17 x 13.5 x 5.5 in
• Weight:5 lbs
• Flow Cells: 8 uL volume and 20 bar pressure max
• Scan Speed: 28 ms/data point
• Step Size: 1 – 20 nm
• Wavelength Repeatability: +/- 0.2 nm
• Wavelength Accuracy: +/- 3 nm
• Single Wavelength Operation: RAMAN (H2O) > 500
• Ex: 350 nm
• Em: 397 nm
• Dark Value: 450 nm
• Dual Wavelength Operation: RAMAN (H2O) > 300
• Ex: 350 nm
• Em: 397 nm
• Dark Value: 450 nm
• Pulse Frequency: 296 Hz for single signal mode. 74 Hz for economy mode
• Maximum Data Rate: 18 Hz
• Excitation Monochromator Range: 200 – 1200 nm
• Bandwidth: 20 nm
• Emission Monochromator Range: 200 – 1200 nm
• Bandwidth: 20 nm

The Waters 2475 Fluorescence Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag. The 2475 FLR Detector sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths, and has the ability to monitor up to four independent wavelength pairs.

Features:

• Innovative axially illuminated flow cell designed for less dispersion, less stray light, less volume, and more pathlength for maximum sensitivity
• Three-dimensional spectral scanning for faster method development and optimization, and enhanced peak identification
• Single or multi-channel operation monitors fluorescence at one or more discrete wavelength pairs
• Low noise performance - Less stray light due to orthogonal optics and the use of mirrors, not lenses, minimize scatter
• Multiple detection modes - 2D, 3D, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence detection methods by quickly determining wavelength maxima
• Integral erbium calibration reference – ensures wavelength accuracy
• Normalized emission units for better bench-to-bench reproducibility

The Thermo Scientific Dionex UltiMate 3000 FLD-3000 Fluorescence Detector series provides high-sensitivity fluorescence detection for UltiMate 3000 HPLC systems. It is available in rapid separation (200 Hz) and standard (100 Hz) versions. The optics of the FLD-3000 Fluorescence Detector series provide maximum stray light suppression for unrivaled detection sensitivity. Innovative technologies supply various tools for individual method development, such as 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition. Operated with Thermo Scientific Dionex Chromeleon Chromatography Data System software, these detectors provide automated qualification, performance optimization, and instrument wellness monitoring for ease of use, maximum uptime, and the highest degree of regulatory compliance.

• Data collection at up to 200 Hz for optimal support of even the shortest UHPLC separations
• Lowest limits of detection with a Raman S/N: > 550 ASTM (> 2100 using dark signal as noise reference)
• Unsurpassed reproducibility with active flow cell temperature control for stable fluorophore activity independent from changes in ambient temperature
• Long-life xenon flash lamp for highest sensitivity and long-term operation without the need for frequent lamp changing
• Optional second photomultiplier for unique Dual-PMT operation offering an extended wavelength range up to 900 nm without sacrificing sensitivity in the standard wavelength range
• 2D or 3D excitation, emission, or synchro scans provide the highest degree in flexibility for method development or routine sample characterization
• Innovative variable emission filter for real-time, compound-related sensitivity optimization (FLD-3400RS only)
• Large front panel display for easy monitoring of detector status
• Flow cell syringe injection kit as a cost-effective solution to perform off-line measurements
• Optimized flow cell design ideal for trace analysis in combination with narrow-bore UHPLC columns and conventional HPLC columns
• Full regulatory compliance with automated qualification period monitoring and automatic tracking of flow cells

Specifications:

• Light Source: Xenon Flash Lamp
• Lamp Pulse Frequency:
  • HighPower (~300 Hz)
  • Standard (~100 Hz)
  • LongLife (~20 Hz)
• Data Collection Rate:
  • Single wavelength: up to 200 Hz (FLD-3400RS)
  • Multiple wavelength: up to 4 Hz (FLD-3400RS)
  • Single wavelength: up to 100 Hz (FLD-3100)
• Spectrum Scanning Modes: 2D or 3D excitation, emission or synchro scans (3D scans under Chromeleon 7.1 control)
• Wavelength Range:   
  • Variant  Ex Min   Ex Max  Em Min Em Max
  • 3100     200       630       265       650
  • 3400RS 200       630       220       650
  • 3100 with Dual-PMT       200       880       265       900
  • 3400RS with Dual-PMT   200       880       220       900
• Spectral Bandwidth:
  • Excitation: 20 nm
  • Emission: 20 nm
• Emission Filter:          Variable, 5 positions (FLD-3400RS) Fixed, 280 nm (FLD-3100)
• of Fluorescence Channels: Up to 4 (FLD-3400RS) 1 (FLD-3100)
• Excitation/Emission Wavelength Switching Time: < 250 ms
• Wavelength Accuracy: ±2 nm
• Wavelength Repeatability: ±0.2 nm
• Sensitivity: Raman S/N: > 550 ASTM over the entire lifetime of the lamp (> 2100 using dark signal as noise reference)
• Flow Cell Thermostatting: Ambient + 10 °C to 50 °C
• Flow Cell:
  • Analytical flow cell volume: 8 μL
  • 20 bar (290 psi) maximum cell pressure
  • Micro flow cell volume: 2 μL
  • 40 bar (580 psi) maximum cell pressure
• PC Connection:           All functions controllable through USB
• GLP Features: Automatic Equipment Qualification (AutoQ), System Wellness, and Qualification Monitoring with Chromeleon, cell ID chip
• Dimensions (h × w × d):
  • 16 × 42 × 51 cm
  • (6.3 × 16.5 × 20 in.)
• Weight: 18 kg

The Thermo Scientific Dionex UltiMate 3000 FLD-3000 Fluorescence Detector series provides high-sensitivity fluorescence detection for UltiMate 3000 HPLC systems. It is available in rapid separation (200 Hz) and standard (100 Hz) versions. The optics of the FLD-3000 Fluorescence Detector series provide maximum stray light suppression for unrivaled detection sensitivity. Innovative technologies supply various tools for individual method development, such as 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition. Operated with Thermo Scientific Dionex Chromeleon Chromatography Data System software, these detectors provide automated qualification, performance optimization, and instrument wellness monitoring for ease of use, maximum uptime, and the highest degree of regulatory compliance.

• Data collection at up to 200 Hz for optimal support of even the shortest UHPLC separations
• Lowest limits of detection with a Raman S/N: > 550 ASTM (> 2100 using dark signal as noise reference)
• Unsurpassed reproducibility with active flow cell temperature control for stable fluorophore activity independent from changes in ambient temperature
• Long-life xenon flash lamp for highest sensitivity and long-term operation without the need for frequent lamp changing
• Optional second photomultiplier for unique Dual-PMT operation offering an extended wavelength range up to 900 nm without sacrificing sensitivity in the standard wavelength range
• 2D or 3D excitation, emission, or synchro scans provide the highest degree in flexibility for method development or routine sample characterization
• Innovative variable emission filter for real-time, compound-related sensitivity optimization (FLD-3400RS only)
• Large front panel display for easy monitoring of detector status
• Flow cell syringe injection kit as a cost-effective solution to perform off-line measurements
• Optimized flow cell design ideal for trace analysis in combination with narrow-bore UHPLC columns and conventional HPLC columns
• Full regulatory compliance with automated qualification period monitoring and automatic tracking of flow cells

Specifications:

• Light Source: Xenon Flash Lamp
• Lamp Pulse Frequency:
  • HighPower (~300 Hz)
  • Standard (~100 Hz)
  • LongLife (~20 Hz)
• Data Collection Rate:
  • Single wavelength: up to 200 Hz (FLD-3400RS)
  • Multiple wavelength: up to 4 Hz (FLD-3400RS)
  • Single wavelength: up to 100 Hz (FLD-3100)
• Spectrum Scanning Modes: 2D or 3D excitation, emission or synchro scans (3D scans under Chromeleon 7.1 control)
• Wavelength Range:   
  • Variant  Ex Min   Ex Max  Em Min Em Max
  • 3100     200       630       265       650
  • 3400RS 200       630       220       650
  • 3100 with Dual-PMT       200       880       265       900
  • 3400RS with Dual-PMT   200       880       220       900
• Spectral Bandwidth:
  • Excitation: 20 nm
  • Emission: 20 nm
• Emission Filter:          Variable, 5 positions (FLD-3400RS) Fixed, 280 nm (FLD-3100)
• of Fluorescence Channels: Up to 4 (FLD-3400RS) 1 (FLD-3100)
• Excitation/Emission Wavelength Switching Time: < 250 ms
• Wavelength Accuracy: ±2 nm
• Wavelength Repeatability: ±0.2 nm
• Sensitivity: Raman S/N: > 550 ASTM over the entire lifetime of the lamp (> 2100 using dark signal as noise reference)
• Flow Cell Thermostatting: Ambient + 10 °C to 50 °C
• Flow Cell:
  • Analytical flow cell volume: 8 μL
  • 20 bar (290 psi) maximum cell pressure
  • Micro flow cell volume: 2 μL
  • 40 bar (580 psi) maximum cell pressure
• PC Connection:           All functions controllable through USB
• GLP Features: Automatic Equipment Qualification (AutoQ), System Wellness, and Qualification Monitoring with Chromeleon, cell ID chip
• Dimensions (h × w × d):
  • 16 × 42 × 51 cm
  • (6.3 × 16.5 × 20 in.)
• Weight: 18 kg

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

• Simplified optical design for optimized baseline stability.
• Long-life Xenon lamp for highest sensitivity.
• Variable-mode monochromators for selective multisignal detection and online spectra acquisition.
• Easy front access to flow cell for fast inspection or exchange.

Specifications

• Detection type:  Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis
• Performance:  10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H20) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
• Light source: Xenon flash lamp, normal mode (20W) and economy mode (5W)
• Pulse frequency: 296 Hz for signal mode, 74 Hz for economy mode
• Excitation monochromator: Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm
• Emission monochromator: Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm
• Reference system: Inline excitation measurement
• Time programing: Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters
• Spectral acquisition: Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step
• Step size: 1—20 nm
• Spectra storage: Limited only by disk space
• Wavelength repeatability: ±0.2 nm
• Wavelength accuracy: ±3 nm
• Flow cells: Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz
• Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, 2 outputs
• Communications: Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.