Used Lab Equipment

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

• Simplified optical design for optimized baseline stability.
• Long-life Xenon lamp for highest sensitivity.
• Variable-mode monochromators for selective multisignal detection and online spectra acquisition.
• Easy front access to flow cell for fast inspection or exchange.

Specifications

• Detection type:  Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis
• Performance:  10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H20) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
• Light source: Xenon flash lamp, normal mode (20W) and economy mode (5W)
• Pulse frequency: 296 Hz for signal mode, 74 Hz for economy mode
• Excitation monochromator: Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm
• Emission monochromator: Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm
• Reference system: Inline excitation measurement
• Time programing: Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters
• Spectral acquisition: Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step
• Step size: 1—20 nm
• Spectra storage: Limited only by disk space
• Wavelength repeatability: ±0.2 nm
• Wavelength accuracy: ±3 nm
• Flow cells: Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz
• Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, 2 outputs
• Communications: Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

 The 1100 HPLC G1321A Fluorescence Detector brings high-sensitivity and selection through simultaneous multi-wavelength detection. This easy-to-use detector provides quantitative data and fluorescence spectra from a single run. The FD's spectral information can be used for rapid method optimization and verification of separation quality.

Specifications:

• Dimensions: 5 x 13.5 x 17 in
• Weight:5 lbs
• Environment:0 to 40°C at <95% humidity
• Performance:10 fg Anthracene, Ex=250 nm, Em=400 nm*
• Performance:RAMAN (H2O) > 200 (FLF rev A)
• Performance:RAMAN (H2O) > 400 (FLF rev >A)
• Performance:Ex=350 nm, Em=397 nm, dark value 450 nm, standard flow cell time constant=4 seconds (8 seconds response time)
• Pulse Frequency:296Hz (single signal mode), 74Hz (spectral mode)
• Excitation Monochromator Range:200 nm - 700 nm
• Excitation Monochromator Bandwidth:20 nm
• Emission Monochromator Range:280 nm - 900 nm
• Emission Monochromator Bandwidth:20 nm
• Spectrum Acquisition Scan Speed:28 ms/datapoint
• Wavelength Characteristic Repeatability:+/- 0.2 nm
• Wavelength Characteristic Accuracy: +/- 3 nm
• Flow cells: 8 uL (standard) and 20 bar (2MPa) pressure maximum

The Waters 2475 Fluorescence Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag. The 2475 FLR Detector sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths, and has the ability to monitor up to four independent wavelength pairs.

Features:

• Innovative axially illuminated flow cell designed for less dispersion, less stray light, less volume, and more pathlength for maximum sensitivity
• Three-dimensional spectral scanning for faster method development and optimization, and enhanced peak identification
• Single or multi-channel operation monitors fluorescence at one or more discrete wavelength pairs
• Low noise performance - Less stray light due to orthogonal optics and the use of mirrors, not lenses, minimize scatter
• Multiple detection modes - 2D, 3D, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence detection methods by quickly determining wavelength maxima
• Integral erbium calibration reference – ensures wavelength accuracy
• Normalized emission units for better bench-to-bench reproducibility

The Waters 474 Scanning Fluorescence Detector can be used for in-vitro diagnostic testing to analyze many compounds, including diagnostic indicators and therapeutically monitored compounds.

Specifications:

• Wavelength Range: 200 - 700 nm
• Spectrum Band Width Excitation: 18 nm
• Spectrum Band Width Emission: 10, 18 or 40 nm
• Wavelength Accuracy: +/- 2 nm
• Wavelength Repeatability: +/- 0.3 nm
• Flow Cell: 16 uL (standard) or 6 uL
• Maximum Flow Cell Pressure: 16 uL, 10 bar; 5 uL, 34.5 bar
• Dimensions: 18.11 x 11.81 x 5.9 in
• Weight: 41.8 lbs
• Temperature Operation: 10 - 32C
• Temperature Storage: 10 - 60C
• Relative Humidity:  20 - 80%

The ACQUITY UPLC TUV Detector is a tunable, dual wavelength ultraviolet/visible (UV/Vis) detector that offers optimal linearity, resolution, and sensitivity for UPLC/UV separations.  It features uniquely designed, patented, light-guiding flow cell technology, low-noise performance (6 μAU), and support for data rates up to 80 Hz.  The ACQUITY UPLC TUV Detector, coupled with our innovative ACQUITY UPLC Column chemistries and separations modules, leverages ultra-low dwell volume and low-noise, high-speed detection to bring productivity, sensitivity, and resolution that are unprecedented in HPLC techniques being used in the laboratory today.

Specifications:

• Wavelength range: 190 to 700 nm
• Wavelength accuracy: ±1 nm
• Linearity range: Deviation at 2.5 AU ≤5%, propylparaben, at 257 nm
• Baseline noise:6 x 10^-3 AU, at 230 nm, 10 mm shunt cell, 1.0 second filter time constant
• Drift: ≤5.0 x 10^-4 AU/hour/°C at 230 nm
• Sampling rate: Up to 80 points/s
• Unattended operation: Leak sensors, full diagnostic data captured through console software
• Light source: Prealigned, intelligent technology Deuterium lamp
• Flow cell design: Light-guiding UPLC flow cell with intelligent chip technology
• Path length: 10 mm (analytical flow cell)
• Cell volume: 500 nL (analytical flow cell)
• Pressure limit: 1000 psi (all flow cells)
• Wetted materials: 316 stainless steel, fused silica, Teflon AF, PEEK (analytical flow cell)
• Optical bandwidth: 5 nm

Features:

• Multiple collection modes: Fluorescence, phosphorescence, chemi/bio-luminescence, and time resolved phosphorescence measurements can all be easily made without changing a single hardware component
• Lowest cost of ownership: The Cary Eclipse is so robust and reliable that every new instrument comes with a 10-year lamp life guarantee, reducing ongoing expenses to virtually zero. 
• Improved laboratory safety: Additionally the guaranteed exceptionally long lifetime of the lamp in the Cary Eclipse eliminates lamp replacements, a hugely time-consuming and potentially hazardous operation.
• Easy to use: Modular, application-specific software, which satisfies the needs of novice and expert users alike, and the ability to switch between measurement modes without touching the hardware, ensure that any user can quickly get reliable, accurate results with minimal training.
• Expand your analytical capabilities: The superior, unique design of the Cary Eclipse ensures room light immunity allowing the instrument to operate with the front and top panels removed for easy access to sample and fast installation of large accessories.
• Proven sensitivity where you need it most: Optimized design coupled with unique, high-sensitivity optics and detectors ensure that the Cary Eclipse gives you the performance that you need right across the spectrum. 
• Robustness, reliability and sensitivity: Engineered to be the most robust instrument on the market, the Cary Eclipse incorporates the unique Agilent xenon flash lamp to capture a data point every 12.5 ms and scans at 24,000 nm/min without peak shifts perfect for high throughput laboratories and fast kinetics applications.
• Unique Sampling: By incorporating Agilent’s unique capacity to guarantee room light immunity coupled with the highly accurate and reproducible Peltier temperature control, the Cary Eclipse delivers unique sampling opportunities that will expand your analytical capabilities, simplify your workflow and increase your sample throughput.
• Reliable Results: The Cary Eclipse only illuminates the sample when data is actually being acquired. This means that the Cary Eclipse prevents your valuable samples from photodegradation and preserves their integrity to the point that you can measure them over and over again and continue to get the same, reliable result. 

Specifications:

• Dimensions(WxDxH): 60 x 64 x 27 cm (23 x 25 x 10 inches)
• Sensitivity:> 500:1 RMS, 500 nm excitation, excitation and emission slits 10 nm / > 750:1 RMS, 350 nm excitation, excitation and emission slits 10 nm
• Wavelength(s):190 - 1100 nm
• Temperature Range: 10 – 35°C
• Optical System: Cerny-Turner 0.125 m Monochromators
• Light Source: Xenon Flash Lamp
• Software: Cary Eclipse Software

Includes:

• Model Eclipse 80i Motorized Microscope
• Mechanical Stage
• (2) CFI10X/22 eyepieces
• Objectives
  • Plan Fluor 10x
  • Plan Fluor 100x OIL
• Motorized Filter Wheel
• Red, Green, Blue cubes
• RETIGA 2000R Q-Imaging Digital Camera
  
  Additional Objectives and Configurations Available. Cameras are also available.

Specifications:

• Shaker: Built-in orbital shaker with adjustable speed and diameter.
• Incubator: Temperature range from RT (25°C) +3°C to 45°C, when the ambient temperature is 25°C. Temperature is selected using Ascent Software.
• Light source: Quartz-halogen lamp. Power consumption 30 W.
• Dimensions: 16.5” x 16.5” x 13.4” (420 x 420 x 340 mm) (W x D x H)
• Weight: 46 lbs (21 kg)

Features:
This is a used Chiu technical fo-150 lumina fiber optic illuminator light source


Specifications:

• Fuse: 5x20mm 3.15 AmpF

• Output: 150 Watts

• Input: 115v 60Hz 3 Amp

Description:

This compact device is the ideal external light source for fluorescence excitation in microscopy. Coupling is preferably achieved via liquid light guides. The average service life of a lamp is 2,000 hours. The optimised internal air flow cools the lamp and enables quiet operation. A resettable operating hour meter shows lamp operating hours. The brightness of the lamp output can be varied in 5 steps (0-100%) by means of a mechanical dimmer unit. An ultra-fast shutter opens or closes after activation with approx. 6 ms. The shutter is actuated by means of an appliance switch, but is also possible externally by means of a remote control input or CAN-BUS connection. This compact light source is available as a VIS or UV version in addition to a version that permits the coupling of multi-pin liquid light guides.

Specifications:

• Dimensions (H x W x D): 210 x 130 x 290 mm
• Weight: 4.7 kg
• Ambient Temperature: 0°C to 40°C
• Storage Temperature: - 20°C to + 85°C

The Lumen 200 Fluorescence Illumination System is a powerful, cost effective alternative to standard 100 Watt mercury and halogen lamphouses. The combination of Prior's proprietary 2,000 hour life, 200 watt metal arc lamp and two or three meter liquid light guide provide unrivalled versatility and illumination power. The Lumen 200 utilizes a metal halide lamp rather than the more traditional high pressure mercury or xenon burners. This means 2000 hours of stable output compared to a maximum of 300 hours for a mercury lamp. 

Specifications:

• Voltage: 100-240V
• Current: 2A
• Frequency: 50-60Hz
• Ambient Operating Temperature: 5 - 30°C
• Weight: 5 kg

The Gemini XPS Fluorescence Microplate Reader uses dual monochromators for variable wavelength selection between 250 nm and 850 nm to eliminate the need for searching out the right pair of excitation and emission filters. Dual monochromators also enable wavelength scanning across a range of wavelengths in increments as small as 1 nm to optimize assay parameters.

Includes:

• Computer loaded with Softmax Pro
• All Necessary Cables and Cords
• Manuals

General Specifications

• Dimensions (in.): 8.6 (h) x 22.8 (w) x 15 (d)
• Dimensions (cm): 22 (h) x 58 (w) x 38 (d)
• Weight: 35 lb (16 kg)
• Power source: 100-240 VAC, 50/60 Hz

Performance Specifications

• Wavelength Range - Excitation: 250-850 nm
• Wavelength Range - Emission: 360-850 nm
• Wavelength Selection: Monochromator, tunable 1.0 nm increments
• Wavelength Bandwidth (Excitation and Emission): 9 nm
• Wavelength Accuracy: < ± 2.0 nm
• Top Read Sensitivity: 3.0 fmol/well FITC 200 µl in 96 wells
• Light Source: Xenon flash lamp
• Microplate Read Time:
  • Normal Mode
  • 96 wells. . . . 15 seconds
  • 384 wells. . . . 45 seconds
• Dynamic Range: 6 orders of magnitude
• Temperature Range: Ambient + 4 °C up to 45 °C
• Temperature Uniformity (Microplate): ± 1 °C @ 37 °C

The Gemini XPS Microplate Reader supports a multitude of fluorescence assay categories including:

• ELISAs and Immunoassays
• Nucleic Acid (DNA) Quantitation
• Protein Quantitation
• Reporter Gene Assays
• Cell Viability, Proliferation, and Cytotoxicity
• Enzyme Assays
• Transporter Assays
• Phosphotases/Kinases
• Microbial Growth

Asking Price: $4500 w/ 1 year manufacturer warranty and 25,000 hour lamp guarantee

List Price: $4995 (We are discounting 10% off List)

Includes: X-Cite 110LED, speedDIAL remote, 3mm x 1500mm Liquid Light Guide, Accessory Kit (tools, USB cable, power cord)

Does not include Microscope Adaptor

The new X-Cite 110LED from Excelitas Technologies is a compact, white light LED light source for fluorescence imaging applications. Providing excitation for DAPI, GFP, mCherry and Cy5 routine imaging, the X-Cite 110LED offers exceptional field uniformity through liquid light guide delivery. With manual, PC and TTL control, the X-Cite 110LED has LEDs guaranteed for 25,000 hours with no lamps or modules to replace. X-Cite 110LED is the newest addition to the X-Cite family which is known for high quality, reliability and exceptional delivery.

Features:

• LED Technology
• White Light Excitation
• Light Guide Delivery
• Multiple Control Options
• Small Footprint
• Optimized Thermal Management
• X-Cite Coupling Optics (optional)

Benefits:

• Mercury-free, higher efficiency, stability, instant ON/OFF and long lifetime
• Excitation of commonly used fluorophores such as DAPI, GFP/FITC, mCherry/Texas Red, and Cy5
• Flexibility in light delivery as well as unit location
• Simple operation through USB, TTL or speedDIAL
• Minimal space requirement; less bench top real estate used
• Maximum LED output, lifetime, stability and reliability
• Efficient optical output and uniformity with the highest performing coupling optics available

Technical Specifications

• Wavelength Range: 360-660nm
• LED Peaks/FWHM: 385/30 nm, 475/40 nm, 560/80 nm, 640/40 nm
• External Power Supply: Universal input 100-240VAC, 50/60Hz
• Power Consumption: 160W
• LED ON/OFF Response Times: 100μs TTL / 1 ms USB
• Control Options:        
  • ON/OFF - TTL compatible
  • speedDIAL
  • RS-232 commands (SDK available), USB
• I/O Connections:      
  • BNC input
  • USB (B-type)
• Dimensions (W x H x D): 95 mm x 230 mm x 212 mm (3.7" x 9.1" x 8.4")
• Weight: 3 kg (6.6 lbs)
• Certifications: CE, RoHS
• Warranty: LEDs – 25,000 hours or 3 years

Includes: Collimating adaptor, Liquid Light Guide

Also Available Used: $2500 without Collimating Adapter

Also ask us about replacement Accessories such as Light Guides and Lamps!

Features

• Pre-aligned lamp
• 2000 hour lamp life, guaranteed
• Powerful 120W lamp
• Intelli-Lamp technology
• Optional adjustable iris

Benefits

• Easy lamp replacement; no alignment required
• Fewer lamp replacements saves time and money
• Rich spectrum with high excitation intensity
• Maintains optimum lamp temperature, automatically accumulates lamp hours
• Minimize photobleaching by adjusting the light intensity of the microscope illumination system

Specifications

• X-Cite 120Q Microscope Illumination System includes: Lamp module, 1.5mm liquid light guide with wide-field fluorescence microscope adaptor, grounded and shielded power cord, and manual
• Lamp: Proprietary 120W Mercury Vapor Short Arc
• Lamp Life: 2000 hours guaranteed; 2500 hours typical
• Power Supply: High efficiency, switch mode, line isolated, 90-264 VAC, 47-63 Hz, universal input
• Warm-Up Period: 90 seconds (typical)
• Panel Controls: Power On/Off, intensity adjustment (if applicable)
• Panel Displays: Accumulated lamp usage, lamp status indicators
• Dimensions (LxWxH): 5″ x 5.5″ x 6.5″ (34.2cm x 13.9cm x 16.5cm)
• Weight: 7 lbs. (3.04 kg)
• Worldwide Certifications: CE Marked, Certified to IEC, Canadian and US Standards
• Warranty: 1 year (excluding lamp and light guide)
• Adaptors: Compatible with wide-field fluorescence microscopes from all major manufacturers

Includes: Collimating adaptor, Liquid Light Guide

Also Available Used: $2500 without Collimating Adapter

Also ask us about replacement Accessories such as Light Guides and Lamps!

Features

• Pre-aligned lamp
• 2000 hour lamp life, guaranteed
• Powerful 120W lamp
• Intelli-Lamp technology
• Optional adjustable iris

Benefits

• Easy lamp replacement; no alignment required
• Fewer lamp replacements saves time and money
• Rich spectrum with high excitation intensity
• Maintains optimum lamp temperature, automatically accumulates lamp hours
• Minimize photobleaching by adjusting the light intensity of the microscope illumination system

Specifications

• X-Cite 120Q Microscope Illumination System includes: Lamp module, 1.5mm liquid light guide with wide-field fluorescence microscope adaptor, grounded and shielded power cord, and manual
• Lamp: Proprietary 120W Mercury Vapor Short Arc
• Lamp Life: 2000 hours guaranteed; 2500 hours typical
• Power Supply: High efficiency, switch mode, line isolated, 90-264 VAC, 47-63 Hz, universal input
• Warm-Up Period: 90 seconds (typical)
• Panel Controls: Power On/Off, intensity adjustment (if applicable)
• Panel Displays: Accumulated lamp usage, lamp status indicators
• Dimensions (LxWxH): 5″ x 5.5″ x 6.5″ (34.2cm x 13.9cm x 16.5cm)
• Weight: 7 lbs. (3.04 kg)
• Worldwide Certifications: CE Marked, Certified to IEC, Canadian and US Standards
• Warranty: 1 year (excluding lamp and light guide)
• Adaptors: Compatible with wide-field fluorescence microscopes from all major manufacturers