Used Lab Equipment

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

• Simplified optical design for optimized baseline stability.
• Long-life Xenon lamp for highest sensitivity.
• Variable-mode monochromators for selective multisignal detection and online spectra acquisition.
• Easy front access to flow cell for fast inspection or exchange.

Specifications

• Detection type:  Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis
• Performance:  10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H20) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
• Light source: Xenon flash lamp, normal mode (20W) and economy mode (5W)
• Pulse frequency: 296 Hz for signal mode, 74 Hz for economy mode
• Excitation monochromator: Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm
• Emission monochromator: Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm
• Reference system: Inline excitation measurement
• Time programing: Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters
• Spectral acquisition: Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step
• Step size: 1—20 nm
• Spectra storage: Limited only by disk space
• Wavelength repeatability: ±0.2 nm
• Wavelength accuracy: ±3 nm
• Flow cells: Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz
• Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, 2 outputs
• Communications: Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

This user-friendly detector brings high sensitivity fluorescence detection for you needs whether used alone or in an automated Agilent 1200 Series system. One run will provide quantitative data and fluorescence spectra all the while improving sensitivity and selectivity with the simultaneous multi-wavelength detection capabilities.

Specifications:

• Environment: 0 – 40°C
• Dimensions: 17 x 13.5 x 5.5 in
• Weight:5 lbs
• Flow Cells: 8 uL volume and 20 bar pressure max
• Scan Speed: 28 ms/data point
• Step Size: 1 – 20 nm
• Wavelength Repeatability: +/- 0.2 nm
• Wavelength Accuracy: +/- 3 nm
• Single Wavelength Operation: RAMAN (H2O) > 500
• Ex: 350 nm
• Em: 397 nm
• Dark Value: 450 nm
• Dual Wavelength Operation: RAMAN (H2O) > 300
• Ex: 350 nm
• Em: 397 nm
• Dark Value: 450 nm
• Pulse Frequency: 296 Hz for single signal mode. 74 Hz for economy mode
• Maximum Data Rate: 18 Hz
• Excitation Monochromator Range: 200 – 1200 nm
• Bandwidth: 20 nm
• Emission Monochromator Range: 200 – 1200 nm
• Bandwidth: 20 nm

The Waters 2475 Fluorescence Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag. The 2475 FLR Detector sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths, and has the ability to monitor up to four independent wavelength pairs.

Features:

• Innovative axially illuminated flow cell designed for less dispersion, less stray light, less volume, and more pathlength for maximum sensitivity
• Three-dimensional spectral scanning for faster method development and optimization, and enhanced peak identification
• Single or multi-channel operation monitors fluorescence at one or more discrete wavelength pairs
• Low noise performance - Less stray light due to orthogonal optics and the use of mirrors, not lenses, minimize scatter
• Multiple detection modes - 2D, 3D, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence detection methods by quickly determining wavelength maxima
• Integral erbium calibration reference – ensures wavelength accuracy
• Normalized emission units for better bench-to-bench reproducibility

The Thermo Scientific Dionex UltiMate 3000 FLD-3000 Fluorescence Detector series provides high-sensitivity fluorescence detection for UltiMate 3000 HPLC systems. It is available in rapid separation (200 Hz) and standard (100 Hz) versions. The optics of the FLD-3000 Fluorescence Detector series provide maximum stray light suppression for unrivaled detection sensitivity. Innovative technologies supply various tools for individual method development, such as 2D or 3D synchro, excitation, emission scans, zero order mode, or multichannel acquisition. Operated with Thermo Scientific Dionex Chromeleon Chromatography Data System software, these detectors provide automated qualification, performance optimization, and instrument wellness monitoring for ease of use, maximum uptime, and the highest degree of regulatory compliance.

• Data collection at up to 200 Hz for optimal support of even the shortest UHPLC separations
• Lowest limits of detection with a Raman S/N: > 550 ASTM (> 2100 using dark signal as noise reference)
• Unsurpassed reproducibility with active flow cell temperature control for stable fluorophore activity independent from changes in ambient temperature
• Long-life xenon flash lamp for highest sensitivity and long-term operation without the need for frequent lamp changing
• Optional second photomultiplier for unique Dual-PMT operation offering an extended wavelength range up to 900 nm without sacrificing sensitivity in the standard wavelength range
• 2D or 3D excitation, emission, or synchro scans provide the highest degree in flexibility for method development or routine sample characterization
• Innovative variable emission filter for real-time, compound-related sensitivity optimization (FLD-3400RS only)
• Large front panel display for easy monitoring of detector status
• Flow cell syringe injection kit as a cost-effective solution to perform off-line measurements
• Optimized flow cell design ideal for trace analysis in combination with narrow-bore UHPLC columns and conventional HPLC columns
• Full regulatory compliance with automated qualification period monitoring and automatic tracking of flow cells

Specifications:

• Light Source: Xenon Flash Lamp
• Lamp Pulse Frequency:
  • HighPower (~300 Hz)
  • Standard (~100 Hz)
  • LongLife (~20 Hz)
• Data Collection Rate:
  • Single wavelength: up to 200 Hz (FLD-3400RS)
  • Multiple wavelength: up to 4 Hz (FLD-3400RS)
  • Single wavelength: up to 100 Hz (FLD-3100)
• Spectrum Scanning Modes: 2D or 3D excitation, emission or synchro scans (3D scans under Chromeleon 7.1 control)
• Wavelength Range:   
  • Variant  Ex Min   Ex Max  Em Min Em Max
  • 3100     200       630       265       650
  • 3400RS 200       630       220       650
  • 3100 with Dual-PMT       200       880       265       900
  • 3400RS with Dual-PMT   200       880       220       900
• Spectral Bandwidth:
  • Excitation: 20 nm
  • Emission: 20 nm
• Emission Filter:          Variable, 5 positions (FLD-3400RS) Fixed, 280 nm (FLD-3100)
• of Fluorescence Channels: Up to 4 (FLD-3400RS) 1 (FLD-3100)
• Excitation/Emission Wavelength Switching Time: < 250 ms
• Wavelength Accuracy: ±2 nm
• Wavelength Repeatability: ±0.2 nm
• Sensitivity: Raman S/N: > 550 ASTM over the entire lifetime of the lamp (> 2100 using dark signal as noise reference)
• Flow Cell Thermostatting: Ambient + 10 °C to 50 °C
• Flow Cell:
  • Analytical flow cell volume: 8 μL
  • 20 bar (290 psi) maximum cell pressure
  • Micro flow cell volume: 2 μL
  • 40 bar (580 psi) maximum cell pressure
• PC Connection:           All functions controllable through USB
• GLP Features: Automatic Equipment Qualification (AutoQ), System Wellness, and Qualification Monitoring with Chromeleon, cell ID chip
• Dimensions (h × w × d):
  • 16 × 42 × 51 cm
  • (6.3 × 16.5 × 20 in.)
• Weight: 18 kg

The Waters 474 Scanning Fluorescence Detector can be used for in-vitro diagnostic testing to analyze many compounds, including diagnostic indicators and therapeutically monitored compounds.

Specifications:

• Wavelength Range: 200 - 700 nm
• Spectrum Band Width Excitation: 18 nm
• Spectrum Band Width Emission: 10, 18 or 40 nm
• Wavelength Accuracy: +/- 2 nm
• Wavelength Repeatability: +/- 0.3 nm
• Flow Cell: 16 uL (standard) or 6 uL
• Maximum Flow Cell Pressure: 16 uL, 10 bar; 5 uL, 34.5 bar
• Dimensions: 18.11 x 11.81 x 5.9 in
• Weight: 41.8 lbs
• Temperature Operation: 10 - 32C
• Temperature Storage: 10 - 60C
• Relative Humidity:  20 - 80%

The ACQUITY UPLC TUV Detector is a tunable, dual wavelength ultraviolet/visible (UV/Vis) detector that offers optimal linearity, resolution, and sensitivity for UPLC/UV separations.  It features uniquely designed, patented, light-guiding flow cell technology, low-noise performance (6 μAU), and support for data rates up to 80 Hz.  The ACQUITY UPLC TUV Detector, coupled with our innovative ACQUITY UPLC Column chemistries and separations modules, leverages ultra-low dwell volume and low-noise, high-speed detection to bring productivity, sensitivity, and resolution that are unprecedented in HPLC techniques being used in the laboratory today.

Specifications:

• Wavelength range: 190 to 700 nm
• Wavelength accuracy: ±1 nm
• Linearity range: Deviation at 2.5 AU ≤5%, propylparaben, at 257 nm
• Baseline noise:6 x 10^-3 AU, at 230 nm, 10 mm shunt cell, 1.0 second filter time constant
• Drift: ≤5.0 x 10^-4 AU/hour/°C at 230 nm
• Sampling rate: Up to 80 points/s
• Unattended operation: Leak sensors, full diagnostic data captured through console software
• Light source: Prealigned, intelligent technology Deuterium lamp
• Flow cell design: Light-guiding UPLC flow cell with intelligent chip technology
• Path length: 10 mm (analytical flow cell)
• Cell volume: 500 nL (analytical flow cell)
• Pressure limit: 1000 psi (all flow cells)
• Wetted materials: 316 stainless steel, fused silica, Teflon AF, PEEK (analytical flow cell)
• Optical bandwidth: 5 nm

Includes:

• Model Eclipse 80i Motorized Microscope
• Mechanical Stage
• (2) CFI10X/22 eyepieces
• Objectives
  • Plan Fluor 10x
  • Plan Fluor 100x OIL
• Motorized Filter Wheel
• Red, Green, Blue cubes
• RETIGA 2000R Q-Imaging Digital Camera
  
  Additional Objectives and Configurations Available. Cameras are also available.

Description:

This compact device is the ideal external light source for fluorescence excitation in microscopy. Coupling is preferably achieved via liquid light guides. The average service life of a lamp is 2,000 hours. The optimised internal air flow cools the lamp and enables quiet operation. A resettable operating hour meter shows lamp operating hours. The brightness of the lamp output can be varied in 5 steps (0-100%) by means of a mechanical dimmer unit. An ultra-fast shutter opens or closes after activation with approx. 6 ms. The shutter is actuated by means of an appliance switch, but is also possible externally by means of a remote control input or CAN-BUS connection. This compact light source is available as a VIS or UV version in addition to a version that permits the coupling of multi-pin liquid light guides.

Specifications:

• Dimensions (H x W x D): 210 x 130 x 290 mm
• Weight: 4.7 kg
• Ambient Temperature: 0°C to 40°C
• Storage Temperature: - 20°C to + 85°C

The Lumen 200 Fluorescence Illumination System is a powerful, cost effective alternative to standard 100 Watt mercury and halogen lamphouses. The combination of Prior's proprietary 2,000 hour life, 200 watt metal arc lamp and two or three meter liquid light guide provide unrivalled versatility and illumination power. The Lumen 200 utilizes a metal halide lamp rather than the more traditional high pressure mercury or xenon burners. This means 2000 hours of stable output compared to a maximum of 300 hours for a mercury lamp. 

Specifications:

• Voltage: 100-240V
• Current: 2A
• Frequency: 50-60Hz
• Ambient Operating Temperature: 5 - 30°C
• Weight: 5 kg

The Gemini XPS Fluorescence Microplate Reader uses dual monochromators for variable wavelength selection between 250 nm and 850 nm to eliminate the need for searching out the right pair of excitation and emission filters. Dual monochromators also enable wavelength scanning across a range of wavelengths in increments as small as 1 nm to optimize assay parameters.

Includes:

• Computer loaded with Softmax Pro
• All Necessary Cables and Cords
• Manuals

General Specifications

• Dimensions (in.): 8.6 (h) x 22.8 (w) x 15 (d)
• Dimensions (cm): 22 (h) x 58 (w) x 38 (d)
• Weight: 35 lb (16 kg)
• Power source: 100-240 VAC, 50/60 Hz

Performance Specifications

• Wavelength Range - Excitation: 250-850 nm
• Wavelength Range - Emission: 360-850 nm
• Wavelength Selection: Monochromator, tunable 1.0 nm increments
• Wavelength Bandwidth (Excitation and Emission): 9 nm
• Wavelength Accuracy: < ± 2.0 nm
• Top Read Sensitivity: 3.0 fmol/well FITC 200 µl in 96 wells
• Light Source: Xenon flash lamp
• Microplate Read Time:
  • Normal Mode
  • 96 wells. . . . 15 seconds
  • 384 wells. . . . 45 seconds
• Dynamic Range: 6 orders of magnitude
• Temperature Range: Ambient + 4 °C up to 45 °C
• Temperature Uniformity (Microplate): ± 1 °C @ 37 °C

The Gemini XPS Microplate Reader supports a multitude of fluorescence assay categories including:

• ELISAs and Immunoassays
• Nucleic Acid (DNA) Quantitation
• Protein Quantitation
• Reporter Gene Assays
• Cell Viability, Proliferation, and Cytotoxicity
• Enzyme Assays
• Transporter Assays
• Phosphotases/Kinases
• Microbial Growth

Asking Price: $4500 w/ 1 year manufacturer warranty and 25,000 hour lamp guarantee

List Price: $4995 (We are discounting 10% off List)

Includes: X-Cite 110LED, speedDIAL remote, 3mm x 1500mm Liquid Light Guide, Accessory Kit (tools, USB cable, power cord)

Does not include Microscope Adaptor

The new X-Cite 110LED from Excelitas Technologies is a compact, white light LED light source for fluorescence imaging applications. Providing excitation for DAPI, GFP, mCherry and Cy5 routine imaging, the X-Cite 110LED offers exceptional field uniformity through liquid light guide delivery. With manual, PC and TTL control, the X-Cite 110LED has LEDs guaranteed for 25,000 hours with no lamps or modules to replace. X-Cite 110LED is the newest addition to the X-Cite family which is known for high quality, reliability and exceptional delivery.

Features:

• LED Technology
• White Light Excitation
• Light Guide Delivery
• Multiple Control Options
• Small Footprint
• Optimized Thermal Management
• X-Cite Coupling Optics (optional)

Benefits:

• Mercury-free, higher efficiency, stability, instant ON/OFF and long lifetime
• Excitation of commonly used fluorophores such as DAPI, GFP/FITC, mCherry/Texas Red, and Cy5
• Flexibility in light delivery as well as unit location
• Simple operation through USB, TTL or speedDIAL
• Minimal space requirement; less bench top real estate used
• Maximum LED output, lifetime, stability and reliability
• Efficient optical output and uniformity with the highest performing coupling optics available

Technical Specifications

• Wavelength Range: 360-660nm
• LED Peaks/FWHM: 385/30 nm, 475/40 nm, 560/80 nm, 640/40 nm
• External Power Supply: Universal input 100-240VAC, 50/60Hz
• Power Consumption: 160W
• LED ON/OFF Response Times: 100μs TTL / 1 ms USB
• Control Options:        
  • ON/OFF - TTL compatible
  • speedDIAL
  • RS-232 commands (SDK available), USB
• I/O Connections:      
  • BNC input
  • USB (B-type)
• Dimensions (W x H x D): 95 mm x 230 mm x 212 mm (3.7" x 9.1" x 8.4")
• Weight: 3 kg (6.6 lbs)
• Certifications: CE, RoHS
• Warranty: LEDs – 25,000 hours or 3 years

Includes: Collimating adaptor, Liquid Light Guide

Also Available Used: $2500 without Collimating Adapter

Also ask us about replacement Accessories such as Light Guides and Lamps!

Features

• Pre-aligned lamp
• 2000 hour lamp life, guaranteed
• Powerful 120W lamp
• Intelli-Lamp technology
• Optional adjustable iris

Benefits

• Easy lamp replacement; no alignment required
• Fewer lamp replacements saves time and money
• Rich spectrum with high excitation intensity
• Maintains optimum lamp temperature, automatically accumulates lamp hours
• Minimize photobleaching by adjusting the light intensity of the microscope illumination system

Specifications

• X-Cite 120Q Microscope Illumination System includes: Lamp module, 1.5mm liquid light guide with wide-field fluorescence microscope adaptor, grounded and shielded power cord, and manual
• Lamp: Proprietary 120W Mercury Vapor Short Arc
• Lamp Life: 2000 hours guaranteed; 2500 hours typical
• Power Supply: High efficiency, switch mode, line isolated, 90-264 VAC, 47-63 Hz, universal input
• Warm-Up Period: 90 seconds (typical)
• Panel Controls: Power On/Off, intensity adjustment (if applicable)
• Panel Displays: Accumulated lamp usage, lamp status indicators
• Dimensions (LxWxH): 5″ x 5.5″ x 6.5″ (34.2cm x 13.9cm x 16.5cm)
• Weight: 7 lbs. (3.04 kg)
• Worldwide Certifications: CE Marked, Certified to IEC, Canadian and US Standards
• Warranty: 1 year (excluding lamp and light guide)
• Adaptors: Compatible with wide-field fluorescence microscopes from all major manufacturers

Includes: Collimating adaptor, Liquid Light Guide

Also Available Used: $2500 without Collimating Adapter

Also ask us about replacement Accessories such as Light Guides and Lamps!

Features

• Pre-aligned lamp
• 2000 hour lamp life, guaranteed
• Powerful 120W lamp
• Intelli-Lamp technology
• Optional adjustable iris

Benefits

• Easy lamp replacement; no alignment required
• Fewer lamp replacements saves time and money
• Rich spectrum with high excitation intensity
• Maintains optimum lamp temperature, automatically accumulates lamp hours
• Minimize photobleaching by adjusting the light intensity of the microscope illumination system

Specifications

• X-Cite 120Q Microscope Illumination System includes: Lamp module, 1.5mm liquid light guide with wide-field fluorescence microscope adaptor, grounded and shielded power cord, and manual
• Lamp: Proprietary 120W Mercury Vapor Short Arc
• Lamp Life: 2000 hours guaranteed; 2500 hours typical
• Power Supply: High efficiency, switch mode, line isolated, 90-264 VAC, 47-63 Hz, universal input
• Warm-Up Period: 90 seconds (typical)
• Panel Controls: Power On/Off, intensity adjustment (if applicable)
• Panel Displays: Accumulated lamp usage, lamp status indicators
• Dimensions (LxWxH): 5″ x 5.5″ x 6.5″ (34.2cm x 13.9cm x 16.5cm)
• Weight: 7 lbs. (3.04 kg)
• Worldwide Certifications: CE Marked, Certified to IEC, Canadian and US Standards
• Warranty: 1 year (excluding lamp and light guide)
• Adaptors: Compatible with wide-field fluorescence microscopes from all major manufacturers

Includes: Collimating adaptor, Liquid Light Guide

Also Available Used: $2500 without Collimating Adapter

Also ask us about replacement Accessories such as Light Guides and Lamps!

Features

• Pre-aligned lamp
• 2000 hour lamp life, guaranteed
• Powerful 120W lamp
• Intelli-Lamp technology
• Optional adjustable iris

Benefits

• Easy lamp replacement; no alignment required
• Fewer lamp replacements saves time and money
• Rich spectrum with high excitation intensity
• Maintains optimum lamp temperature, automatically accumulates lamp hours
• Minimize photobleaching by adjusting the light intensity of the microscope illumination system

Specifications

• X-Cite 120Q Microscope Illumination System includes: Lamp module, 1.5mm liquid light guide with wide-field fluorescence microscope adaptor, grounded and shielded power cord, and manual
• Lamp: Proprietary 120W Mercury Vapor Short Arc
• Lamp Life: 2000 hours guaranteed; 2500 hours typical
• Power Supply: High efficiency, switch mode, line isolated, 90-264 VAC, 47-63 Hz, universal input
• Warm-Up Period: 90 seconds (typical)
• Panel Controls: Power On/Off, intensity adjustment (if applicable)
• Panel Displays: Accumulated lamp usage, lamp status indicators
• Dimensions (LxWxH): 5″ x 5.5″ x 6.5″ (34.2cm x 13.9cm x 16.5cm)
• Weight: 7 lbs. (3.04 kg)
• Worldwide Certifications: CE Marked, Certified to IEC, Canadian and US Standards
• Warranty: 1 year (excluding lamp and light guide)
• Adaptors: Compatible with wide-field fluorescence microscopes from all major manufacturers

The X-Cite 120LEDBoost is more powerful than ever and efficiently excites a greater range of fluorophores than the original X-Cite 120LED, with no compromise. Even the popular red fluorophores are no challenge for the X-Cite 120LEDBoost. The X-Cite 120LEDBoost offers superior optical power and exceptional field uniformity at the specimen level with the broadest spectrum of fluorescence illumination through manual, PC and TTL control. With LEDs guaranteed for 25,000 hours and no lamps or modules to replace, X-Cite 120LEDBoost offers sheer simplicity and convenience to researchers allowing them to focus on their experiments instead of equipment maintenance.

Including microscope flange (direct coupled), PC/TTL control and a speed dial hand controller for easy manual on/off and 1% intensity steps

Features

• High Power LEDs
• Broad Spectrum
• Excellent Field Uniformity
• Instant ON/OFF (electronic shuttering)
• Several Control Options
• Direct Coupling
• LED Technology
• Unmatched Thermal Management
• Simple Operation
• Less Toxic Waste

Benefits

• Efficient fluorophore excitation
• Excitation of a variety of fluorophores
• Reliable image data
• No warm-up, no vibration from mechanical shuttering
• Manual, USB, TTL, 3rd party software
• Maximum light transmission to sample
• Long-life, stable, reliable, maintenance-free
• Vibration-free imaging
• Fingertip control, no training required
• No mercury disposal issues

Specifications:

• Wavelength Range: 370-700nm
• Power Supply: Universal input 100-240VAC, 50/60Hz
• Power Consumption: 230W
• LED ON/OFF: 50µs TTL
• Response Times: 1ms USB
• Control Options
  • Manual – speedDIAL (1% increments)
  • TTL compatible – BNC input
  • RS-232 commands (SDK available)
  • USB
• Height
  • powerCUBE: 213mm (8.4″)
  • LED Head: 116mm (4.6″)
  • speedDIAL: 59mm (2.3″)
• Width
  • powerCUBE: 173mm (6.8″)
  • LED Head: 90mm (3.5″)
  • speedDIAL: 80mm (3.1″)
• Depth
  • powerCUBE: 260mm (10.2″)
  • LED Head: 166mm (6.5″)
  • speedDIAL: 112mm (4.4″)
• Weight
  • powerCUBE: 4.8kg (10.6lbs)
  • speedDIAL: 0.3kg (0.7lbs)
• Certifications: CE marked; certified to IEC, Canadian and US standards; RoHS2 compliant
• Warranty: LEDs – 25,000 hours (or 3 years); all other X-Cite® 120LED components 1 year, parts and labor

**Brand New**