Used Lab Equipment

The ACQUITY UPLC TUV Detector is a tunable, dual wavelength ultraviolet/visible (UV/Vis) detector that offers optimal linearity, resolution, and sensitivity for UPLC/UV separations.  It features uniquely designed, patented, light-guiding flow cell technology, low-noise performance (6 μAU), and support for data rates up to 80 Hz.  The ACQUITY UPLC TUV Detector, coupled with our innovative ACQUITY UPLC Column chemistries and separations modules, leverages ultra-low dwell volume and low-noise, high-speed detection to bring productivity, sensitivity, and resolution that are unprecedented in HPLC techniques being used in the laboratory today.

Specifications:

• Wavelength range: 190 to 700 nm
• Wavelength accuracy: ±1 nm
• Linearity range: Deviation at 2.5 AU ≤5%, propylparaben, at 257 nm
• Baseline noise:6 x 10^-3 AU, at 230 nm, 10 mm shunt cell, 1.0 second filter time constant
• Drift: ≤5.0 x 10^-4 AU/hour/°C at 230 nm
• Sampling rate: Up to 80 points/s
• Unattended operation: Leak sensors, full diagnostic data captured through console software
• Light source: Prealigned, intelligent technology Deuterium lamp
• Flow cell design: Light-guiding UPLC flow cell with intelligent chip technology
• Path length: 10 mm (analytical flow cell)
• Cell volume: 500 nL (analytical flow cell)
• Pressure limit: 1000 psi (all flow cells)
• Wetted materials: 316 stainless steel, fused silica, Teflon AF, PEEK (analytical flow cell)
• Optical bandwidth: 5 nm

Specification:

• Detection type: Double-beam photometer
• Light source: Deuterium lamp
• Wavelength range: 190–600 nm
• Short term noise (ASTM): ±0.75×10^-5 AU at 254 nm
• Drift: 3 × 10 ^-4 AU/hr at 254 nm
• Linearity: 2 AU (5%) upper limit
• Wavelength accuracy:
• Band width: 5 nm typical
• Flow cells:
  • Standard: 14-μl volume, 10-mm cell path length and 40 bar (588 psi) pressure maximum
  • High pressure: 14-μl volume, 10-mm cell path length and 400 bar (5880 psi) pressure maximum
  • Micro: 1-μl volume, 5-mm cell path length and 40 bar (588 psi) pressure maximum
  • Semimicro: 5-μl volume, 6-mm cell path length and 40 bar (588 psi) pressure maximum
• Control and data evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, one output
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and Agilent ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter
• Housing: All materials recyclable.

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

The Agilent 1100 Series G1315 Diode Array Detector is designed for the maximum optical performance. Flexible slit setting from fine bands to highest sensitivity, with settings of 1 nm the fine structure of benzene can be revealed - even at 0.7mAUFS. A broad slit of 8 or 16nm can be chosen to minimize baseline noise thus enhancing sensitivity. System specifications include:

Specifications:

• Wavelength range: 190-950 nm
• Light source: Deuterium and tungsten lamps
• Detection type: 1024 - element photodiode array
• Short term noise (ASTM) single & mult-wavelength: ± 1° x 10-5 AU at 254 and 750 nm
• Drift: 2° x 10-3 AU / hr at 254 nm
• Linear absorbance range: > 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 - 400 nm
• Diode width: < 1 nm
• Control and data evaluation: Agilent Chemstation for LC
• Slit width: 1, 2, 4, 8, 16 nm

Can be sold as an individual component or as part of a complete stack

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

• Simplified optical design for optimized baseline stability.
• Long-life Xenon lamp for highest sensitivity.
• Variable-mode monochromators for selective multisignal detection and online spectra acquisition.
• Easy front access to flow cell for fast inspection or exchange.

Specifications

• Detection type:  Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis
• Performance:  10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H20) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
• Light source: Xenon flash lamp, normal mode (20W) and economy mode (5W)
• Pulse frequency: 296 Hz for signal mode, 74 Hz for economy mode
• Excitation monochromator: Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm
• Emission monochromator: Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm
• Reference system: Inline excitation measurement
• Time programing: Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters
• Spectral acquisition: Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step
• Step size: 1—20 nm
• Spectra storage: Limited only by disk space
• Wavelength repeatability: ±0.2 nm
• Wavelength accuracy: ±3 nm
• Flow cells: Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz
• Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, 2 outputs
• Communications: Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

Specifications:

• Detection type: 1024-element photodiode array
• Light source: Deuterium and tungsten lamps
• Wavelength range: 190 – 950 nm
• Short term noise (ASTM) Single and Multi-Wavelength: ± 1 × 10-5AU at 254 and 750 nm
• Drift: 2 × 10-3AU/hr at 254 nm
• Linear absorbance Range: 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 – 400 nm
• Slit width: 1, 2, 4 , 8, 16 nm
• Diode width: < 1 nm
• Flow cells
  • Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
  • Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
  • High pressure: 1.7 µl volume, 6 mm cellpath length and 400 bar (5880 psi) pressure maximum
  • 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
• Control and data Evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter

Can be sold as an individual component or as part of a complete stack

Specifications:

• Detection type: 1024-element photodiode array
• Light source: Deuterium and tungsten lamps
• Wavelength range: 190 – 950 nm
• Short term noise (ASTM) Single and Multi-Wavelength: ± 1 × 10-5AU at 254 and 750 nm
• Drift: 2 × 10-3AU/hr at 254 nm
• Linear absorbance Range: 2 AU (upper limit)
• Wavelength accuracy: ± 1 nm
• Wavelength bunching: 1 – 400 nm
• Slit width: 1, 2, 4 , 8, 16 nm
• Diode width: < 1 nm
• Flow cells
  • Standard: 13 µl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum
  • Semi-Micro: 5 µl volume, 6 mm cell path length and 120 bar (1760 psi) pressure maximum
  • High pressure: 1.7 µl volume, 6 mm cellpath length and 400 bar (5880 psi) pressure maximum
  • 500 nano: 0.5 µl volume, 10 mm cell path length and 50 bar (725 psi) pressure maximum
• Control and data Evaluation: Agilent ChemStation for LC
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range 0.001 – 2 AU, two outputs
• Communications: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and shut-down signals, LAN optional
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.
• GLP features: Early maintenance feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user-settable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter

Can be sold as an individual component or as part of a complete stack

Specifications: 

• Detection type: Double-beam photometer
• Light source: Deuterium lamp
• Noise: ± 0.75 x 10 -5 AU, at 254 nm
• Drift: 3 x 10-4AU/hr, at 254 nm
• Linearity: >2 AU upper limit
• Wavelength range: 190-600 nm
• Wavelength accuracy: ± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter
• Wavelength bandwidth: 6.5 nm typical over whole wavelength range
• Maximum data rate: 55 Hz

This user-friendly detector brings high sensitivity fluorescence detection for you needs whether used alone or in an automated Agilent 1200 Series system. One run will provide quantitative data and fluorescence spectra all the while improving sensitivity and selectivity with the simultaneous multi-wavelength detection capabilities.

Specifications:

• Environment: 0 – 40°C
• Dimensions: 17 x 13.5 x 5.5 in
• Weight:5 lbs
• Flow Cells: 8 uL volume and 20 bar pressure max
• Scan Speed: 28 ms/data point
• Step Size: 1 – 20 nm
• Wavelength Repeatability: +/- 0.2 nm
• Wavelength Accuracy: +/- 3 nm
• Single Wavelength Operation: RAMAN (H2O) > 500
• Ex: 350 nm
• Em: 397 nm
• Dark Value: 450 nm
• Dual Wavelength Operation: RAMAN (H2O) > 300
• Ex: 350 nm
• Em: 397 nm
• Dark Value: 450 nm
• Pulse Frequency: 296 Hz for single signal mode. 74 Hz for economy mode
• Maximum Data Rate: 18 Hz
• Excitation Monochromator Range: 200 – 1200 nm
• Bandwidth: 20 nm
• Emission Monochromator Range: 200 – 1200 nm
• Bandwidth: 20 nm

Features:

• Advanced temperature controlled detector optics ready to use within two hours of installation
• Automatic zero and automatic purge combined with a recycle valve for automatic solvent recycling allow uninterrupted operation
• Automatic light intensity control circuit to ensure the optimum performance of the optics
• Integrated diagnostics for efficient troubleshooting
• Built-in refractive index calibration
• Front access to valves and capillaries for easy maintenance
•  

Specifications:

• Detection type: Refractive Index
• Refractive index range: 1.00 – 1.75 RIU, calibrated
• Measurement range: +/- 600 x 10-6 RIU
• Optics temperature control: 5 ° C above ambient to 55 ° C
• Sample cell volume: 8uL
• Maximum pressure: 5 bar (0.5Mpa)
• Maximum flow rate: 5mL/minute
• Valves: Automatic purge and automatic solvent recycle
• Volumes: Inlet port to sample cell 62uL, inlet port to outlet port 590uL

Features:

The Agilent 1260 Infinity II Diode Array Detector WR offers multiple wavelengths and full spectral detection at up to120 Hz sampling rate, keeping up with the analysis speed of fast LC. Even at high data rates, the 1260 Infinity II DAD WR enables precise identification, quantification, and peak purity analysis at trace levels. Maximum flexibility is guaranteed with a wide wavelength range of 190 to 950 nm with dual-lamp design, as well as the choice of 12 analytical, preparative and SFC flow cells.

Specifications:

• Fast data acquisition - full spectral data acquisition rate of 120 Hz increases resolution and delivers fast, highly sensitive peak purity analysis and spectral confirmation – at trace levels.
• Highest sensitivity - dual-lamp design allows analysis of wavelengths between190 to 950 nm.
• Simultaneous data acquisition - The diode array design enables simultaneous acquisition of up to eight compound-specific wavelengths for increased sensitivity and selectivity.
• Rapid optimization of sensitivity, linearity and spectral resolution-programmable slit (1 to 16 nm) provides optimum incident light conditions.
• Eliminates background interference- with reference wavelengths.
• Minimized short-term noise (< ± 7 µAU ASTM) - low noise front-end electronics and special flow cell design deliver lowest detection limits.
• Maximum flexibility, compatibility and investment protection – with a range of 12 analytical, preparative and SFC flow cells.

The 1290 Infinity Diode Array Detector (DAD) features a completely new optical design based on the Agilent Max-Light cartridge cell with optofluidic waveguides. With typical detector noise levels of < ± 0.6 μAU/cm the revolutionary 6 cm flow cell gives up to 10 times higher sensitivity than the 1200 Series DAD and VWD.  Any compromising refractive index and thermal effects are almost completely eliminated,

resulting in significantly less baseline drift for more reliable and precise peak integration. For ultra high productivity the 1290 Infinity DAD offers multiple wavelength and full spectral detection at sampling rates up to 160 Hz.

Features:

• Ultra sensitivity through revolutionary Agilent Max-Light cartridge cell with 60 mm
• optical path length (typically noise: < ± 0.6 μAU/cm).
• Universal Agilent Max-Light cartridge standard cell with 10 mm optical path length
• provides high sensitivity (noise: < ± 3 μAU) and lowest peak dispersion for 2.1, 3
• and 4.6 mm ID columns.
• Programmable slit from 1 to 8 nm provides optimum incident light conditions for
• rapid optimization of sensitivity, linearity and spectral resolution.
• Multiple wavelength and full spectral detection at high sampling rate of 160 Hz,
• keeping pace with fastest possible analysis speed.
• More reliable and robust peak integration process due through less baseline drift.
• Full spectral detection for compound identification by spectral libraries or verification
• of the separation quality with peak purity analysis for ultra fast LC. Simultaneous
• detection of up to 8 signals for increased sensitivity and selectivity.
• Wide linear range (typically up to 2.5 AU) – for reliable, simultaneous quantification
• of primary compounds, by-products and impurities.
• Radio frequency identification (RFID) technology for flow cells and lamp provide
• new levels of data security and traceability.
• Electronic temperature control (ETC) provides maximum baseline stability and
• practical sensitivity under fluctuating ambient temperature and humidity conditions.
• Reference wavelength for elimination of background interference.

The Waters 2475 Fluorescence Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag. The 2475 FLR Detector sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths, and has the ability to monitor up to four independent wavelength pairs.

Features:

• Innovative axially illuminated flow cell designed for less dispersion, less stray light, less volume, and more pathlength for maximum sensitivity
• Three-dimensional spectral scanning for faster method development and optimization, and enhanced peak identification
• Single or multi-channel operation monitors fluorescence at one or more discrete wavelength pairs
• Low noise performance - Less stray light due to orthogonal optics and the use of mirrors, not lenses, minimize scatter
• Multiple detection modes - 2D, 3D, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence detection methods by quickly determining wavelength maxima
• Integral erbium calibration reference – ensures wavelength accuracy
• Normalized emission units for better bench-to-bench reproducibility

The Waters 2487 Dual λ Absorbance Detector is a two-channel, tunable, ultraviolet/visible (UV/Vis) detector designed for high-performance liquid chromatography (HPLC) applications. The Waters 2487 Dual λ Absorbance Detector can operate either as a stand-alone unit (with a chart recorder or integrator) or as an integral part of a Waters chromatography system.

Specifications:

• Wavelength range: 190 to 700 nm
• Bandwidth: <5 nm
• Wavelength accuracy: +1.0 nm
• Wavelength repeatability: +0.1 nm
• Single λ dry noise: <5 µAU (at 230 nm, 1 –second digital filter, 30-second segments, 10-Hz data rate, analytical cell)
• Wet noise: <8 µAU (at 230 nm, 1-second digital filter 30-second segments, 10-Hz data rate, analytical cell)
• Dual channel noise (dry): <35 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; dry, analytical cell)
• Dual channel noise (wet): <45 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; 1 ml/min, flow of acetonitrile, analytical cell)
• Linearity: <5% at 2.5 AU, propylparaben at 257 nm
• Drift: 1.0 x 10-4 AU/hr. max (∆T = +2°C/hr.)
• Thermal drift: 1.0 x 10-4 AU/°C max (∆T = +2°C/hr.)
• Sensitivity setting range: 0.0001 to 4.0000 AUFS
• Filter setting range
  • Single-wavelength: 0.1 to 5.0 seconds
  • Dual-wavelength: 1 to 50 seconds
• Time-constant: To 5.0-second Hamming filter
• Digital data rates:
  • 1,2,5,10,20,40,80 Hz (single channel)
  • 1,2 Hz (dual channel)
• Analog output data rates (single λ mode):
  • 10,20,40,80 Hz (channel A)
  • 10 Hz only (channel B)
• Lamp source: 30-W High Brightness Deuterium lamp, 0.5 nm aperture, pre-aligned 2000 silicon photodiodes (matched pair)
• Second-order filter: Automatic for wavelengths > 370 nm
• Wavelength calibration filter: Erbium filter, used at startup or on demand
• Flow cell: TaperSlit flow cell design
• Nitrogen purge: Purge fitting present on optics bench
• Path length: 0 mm (standard analytical)
• Cell volume: 10 µL (standard analytical)
• Pressure limit: 6895 kPa (69 bar,1000 psi)
• Materials: 316 stainless steel, fused silica, Tygon
• Operating temperature: 4 to 40 °C (39 to 104 °F)
• Operating humidity: 20% to <95%, noncondensing
  
  

Waters 2996 Photo Diode Array (PDA) Detector has uniquely-integrated software and optics innovations that deliver no-compromise chromatographic and spectral sensitivity, reliability and ease of use for analytical and preparative separations. Advanced spectral analysis enhances component recognition and homogeneity assessment.

Features:

• Maximum chromatographic sensitivity means unprecedented trace impurity detection and quantification.
• Optimized spectral sensitivity and Waters purity algorithms mean definitive compound identification and co-elution detection.
• Superior linearity range defined by a fixed optical bandpass
• Simplified optics that are reliable and easy to maintain
• Method development and validation are fast and straight forward with uncomplicated method development tools within Empower
• Compliance-ready for the FDA Electronic Records and Electronic Signatures Rule
• Start-up diagnostics that ensure detector performance prior to use

Specification:

• Linearity range: 5% over -0.1 to 2.0 AU
• Spectral resolution: 1.2 nm per photodiode
• Baseline noise: 5 x 10 -5 AU peak-to-peak
• Drift: 1 x 10 -3 AU/hour at 254 nm (after warmup)
• Operating temperature: 4 to 40 ¡ãC (39 to 104 ¡ãF)
• Operating humidity: 20 to 80% non-condensing
• Wavelength accuracy: 1nm
• Wavelength range: 190 to 800 nm