Used Lab Equipment

The Invitrogen PowerEase 500 Power Supply is a microprocessor-controlled power supply for electrophoresis of pre-cast and hand-poured mini-gels. By combining high performance and programming flexibility this unit simplifies electrophoresis. This unit features: large LCD display, pre-programmed and custom methods, three step operating procedure, and a parallel port.

Specifications:

• Output Range: 1–500 V (minimum step size 1 V), 1–500 mA (minimum step size 1 mA), 0.1–50 W (minimum step size 0.1 W), Automatic crossover on reaching set limits.
• Accuracy: Voltage: ± 2% or ± 2 volts, Current: ± 2% or ± 2 mA, Wattage ± 2% or ± 0.2 watt
• Drift: < 1% in 8 hours after 30 minute warm-up with constant supply voltage
• Circuit Protection: Open Circuit; Short Circuit; Thermal Protection Over Voltage, Current, Wattage
• Safety: Load Detection, Thermal Shutoff (with optional temperature probe), Isolated Floating Ground
• Memory Control: Non-volatile, up to 24 hours, Custom Methods Stored to RAM
• Computer Interface: RS232C
• Display: LCD, view area 114 x 64 mm, 240 × 128 dots
• Dimensions (W x H x D): 16.5 cm × 18 cm × 20.5 cm
• Weight: 2.9 kg (6.4 lbs)
• Operating Environmental Conditions: Ambient temperature 4–30°C, ≤80% relative, humidity, altitude < 2000 meters, indoor use only, pollution degree

Description:

The E-Gel iBase Power System has an LCD display, which shows information about the program selected and running time. The display is located near the upper edge of the iBase device. Just below the display, the E-Gel iBase Power System has four buttons:

• Go button starts programs
• Mode button toggles between programs, minutes, and seconds
• Up button selects between programs on the display and increases running time
• Down button selects between programs on the display and decreases running time

An LED light (in the middle of the four buttons) indicates the status of the iBase device. The gel cassette is inserted into the two Electrode connections at the lower right of the iBase Power System. The back of the E-Gel iBase Power System contains a USB port and a power inlet. The supplied power cord has a matching connector that inserts into the power inlet, and connects the E-Gel® iBase™ Power System to the electrical outlet. A separate, stand-alone power supply is not required to run the iBase™ device.

Specification:

• Dimensions: 18.4 cm x 11 cm x 5.75 cm
• Weight: 500 g
• Electrical requirements: 100–240 V AC, 50/60 Hz< 1.0 A
• Temperature: 5°C to 40°C
• Built-in features: LCD Display, Alarm , LED light

Description:

The E-Gel Safe Imager Real-time Transilluminator is designed for viewing E-Gel with SYBR Safe gels and E-Gel CloneWell gels on the laboratory benchtop for real-time monitoring on the E-Gel iBase Power System or for documentation purposes at the end of the run directly on the E-Gel Safe Imager Transilluminator. The E-Gel Safe Imager Real-time Transilluminator has the following features:

• An array of 12 LED sources behind a blue filter that emit high intensity blue light.
• Red ON/OFF button is located at the front.
• Automatic shut-off option at 30 seconds and 5 minutes
• LED indicator light adjacent to the ON/OFF button indicates the status of the Safe Imager unit.
• Attached short electrical cord connects to the iBase unit.
• USB port enables future program updates.

Specifications:

• Viewing surface dimensions: 62 mm x 77 mm
• Case dimensions: 200 mm x 110 mm x 43 mm
• Amber filter dimensions: 121 mm x 138 mm x 31 mm
• Weight of Safe Imager: 243 g
• Weight of filter: 55 g
• Temperature: 5°C to 40°C
• Built in features: LED light
• LED life: 50,000 hours
• LED specifications: Array of 12 high power LEDs emitting at 480 ± 5 nm. LEDs radiate <10 Lumens each at 200 mA.

Description:

The iBind™ is a benchtop device utilizing sequential lateral flow (SLF) to perform hands-free blocking, antibody binding, and washes for western detection workflows. The iBind™ uses no external power source, and relies on mechanical pressure from the iBind™ on a iBind™ Card to generate the sequential flow of immunodetection reagents for performing the blocking, antibody binding, and wash steps involved in western detection workflows

Specifications:

• Dimensions (L x W x H): 24.2 x 14.6 x 3.5 cm
• Material: Aluminum, plastic (PC/ABS), steel, silicone, neodymium (magnets)
• Operating Temperature: 18ËšC to 30ËšC

The revolutionary iBlot Dry Blotting System transfers proteins from polyacrylamide gels to membranes in seven minutes or less. The iBlot® western blotting system offers high transfer quality for downstream immunodetection sensitivities.

• Fast— protein transfer in 7 minutes or less
• High quality transfer— efficient transfers without bubbles or distortions
• Convenient—Self contained unit without the need for additional buffer or external power supply
• Reproducible—reduces blot preparation and running variability

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting