Used Lab Equipment

Description:

The E-Gel iBase Power System has an LCD display, which shows information about the program selected and running time. The display is located near the upper edge of the iBase device. Just below the display, the E-Gel iBase Power System has four buttons:

• Go button starts programs
• Mode button toggles between programs, minutes, and seconds
• Up button selects between programs on the display and increases running time
• Down button selects between programs on the display and decreases running time

An LED light (in the middle of the four buttons) indicates the status of the iBase device. The gel cassette is inserted into the two Electrode connections at the lower right of the iBase Power System. The back of the E-Gel iBase Power System contains a USB port and a power inlet. The supplied power cord has a matching connector that inserts into the power inlet, and connects the E-Gel® iBase™ Power System to the electrical outlet. A separate, stand-alone power supply is not required to run the iBase™ device.

Specification:

• Dimensions: 18.4 cm x 11 cm x 5.75 cm
• Weight: 500 g
• Electrical requirements: 100–240 V AC, 50/60 Hz< 1.0 A
• Temperature: 5°C to 40°C
• Built-in features: LCD Display, Alarm , LED light

Description:

The iBind™ is a benchtop device utilizing sequential lateral flow (SLF) to perform hands-free blocking, antibody binding, and washes for western detection workflows. The iBind™ uses no external power source, and relies on mechanical pressure from the iBind™ on a iBind™ Card to generate the sequential flow of immunodetection reagents for performing the blocking, antibody binding, and wash steps involved in western detection workflows

Specifications:

• Dimensions (L x W x H): 24.2 x 14.6 x 3.5 cm
• Material: Aluminum, plastic (PC/ABS), steel, silicone, neodymium (magnets)
• Operating Temperature: 18ËšC to 30ËšC

The revolutionary iBlot Dry Blotting System transfers proteins from polyacrylamide gels to membranes in seven minutes or less. The iBlot® western blotting system offers high transfer quality for downstream immunodetection sensitivities.

• Fast— protein transfer in 7 minutes or less
• High quality transfer— efficient transfers without bubbles or distortions
• Convenient—Self contained unit without the need for additional buffer or external power supply
• Reproducible—reduces blot preparation and running variability

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

The Invitrogen PowerEase 500 Power Supply is a microprocessor-controlled power supply for electrophoresis of pre-cast and hand-poured mini-gels. By combining high performance and programming flexibility this unit simplifies electrophoresis. This unit features: large LCD display, pre-programmed and custom methods, three step operating procedure, and a parallel port.

Specifications:

• Output Range: 1–500 V (minimum step size 1 V), 1–500 mA (minimum step size 1 mA), 0.1–50 W (minimum step size 0.1 W), Automatic crossover on reaching set limits.
• Accuracy: Voltage: ± 2% or ± 2 volts, Current: ± 2% or ± 2 mA, Wattage ± 2% or ± 0.2 watt
• Drift: < 1% in 8 hours after 30 minute warm-up with constant supply voltage
• Circuit Protection: Open Circuit; Short Circuit; Thermal Protection Over Voltage, Current, Wattage
• Safety: Load Detection, Thermal Shutoff (with optional temperature probe), Isolated Floating Ground
• Memory Control: Non-volatile, up to 24 hours, Custom Methods Stored to RAM
• Computer Interface: RS232C
• Display: LCD, view area 114 x 64 mm, 240 × 128 dots
• Dimensions (W x H x D): 16.5 cm × 18 cm × 20.5 cm
• Weight: 2.9 kg (6.4 lbs)
• Operating Environmental Conditions: Ambient temperature 4–30°C, ≤80% relative, humidity, altitude < 2000 meters, indoor use only, pollution degree

Description:

The E-Gel Safe Imager Real-time Transilluminator is designed for viewing E-Gel with SYBR Safe gels and E-Gel CloneWell gels on the laboratory benchtop for real-time monitoring on the E-Gel iBase Power System or for documentation purposes at the end of the run directly on the E-Gel Safe Imager Transilluminator. The E-Gel Safe Imager Real-time Transilluminator has the following features:

• An array of 12 LED sources behind a blue filter that emit high intensity blue light.
• Red ON/OFF button is located at the front.
• Automatic shut-off option at 30 seconds and 5 minutes
• LED indicator light adjacent to the ON/OFF button indicates the status of the Safe Imager unit.
• Attached short electrical cord connects to the iBase unit.
• USB port enables future program updates.

Specifications:

• Viewing surface dimensions: 62 mm x 77 mm
• Case dimensions: 200 mm x 110 mm x 43 mm
• Amber filter dimensions: 121 mm x 138 mm x 31 mm
• Weight of Safe Imager: 243 g
• Weight of filter: 55 g
• Temperature: 5°C to 40°C
• Built in features: LED light
• LED life: 50,000 hours
• LED specifications: Array of 12 high power LEDs emitting at 480 ± 5 nm. LEDs radiate <10 Lumens each at 200 mA.

Andor’s new iXon Life EMCCD (Electron Multiplying CCD) platform is available exclusively for fluorescence microscopy applications and is engineered to deliver single photon sensitivity with absolutely unparalleled price/performance. The ideal detector to drive superlative performance from confocal spinning disk technology. Whilst affording superb confocality and low rates of phototoxicity, spinning disk experiments are inherently photon starved, by virtue of the photon rejection implicit to optical sectioning. The superior sensitivity of the iXon Life detector brings these low light images to life.

Specifications:

• Sensor Type: Back Illuminated, standard AR coated
• Active pixels (H x V): 1024 x 1024
• Pixel Size (H x V: µm): 13 x 13
• Image Area: 13.3 x 13.3 mm with 100% Fill Factor
• Active Area Pixel Well Depth (e-): 80,000 180,000
• Pixel Readout Rate
• Minimum Temperature, air cooled, ambient 20°C: 10 MHz -70ºC, 30 MHz•3 -55ºC
• Chiller Liquid Cooling, coolant @ 10°C, >0.75l/min: 10 MHz -80ºC, 30 MHz•3 -65ºC
• Thermostatic Precision: ± 0.01°C
• Triggering: Internal, External, External Start, External Exposure, Software Trigger
• System Window Type: UV-grade fused silica, Broadband Visible-Near Infrared, 0.5 degree wedge
• Digitization: 16-bit (at all speeds)
• PC Interface: USB 3.0•12
• Lens Mount: C-mount
• Dark Current and Background Events•4,5:
• Dark Current (e- /pixel/sec) @ -80°C: 0.00025
• Spurious Background (events/pix) @ 1000x gain / -80°C: 0.005
• Active Area Pixel Well Depth: 80,000 e-
• Gain Register Pixel Well Depth•6: 730,000 e-
• Read noise (e-) •7: <1
• Linearity•8: Better than 99.9%
• Vertical Clock Speed: 0.6 to 4.33 µs (user selectable)
• Timestamp accuracy: 10 ns

Andor’s new iXon Life EMCCD (Electron Multiplying CCD) platform is available exclusively for fluorescence microscopy applications and is engineered to deliver single photon sensitivity with absolutely unparalleled price/performance. The ideal detector to drive superlative performance from confocal spinning disk technology. Whilst affording superb confocality and low rates of phototoxicity, spinning disk experiments are inherently photon starved, by virtue of the photon rejection implicit to optical sectioning. The superior sensitivity of the iXon Life detector brings these low light images to life.

Specifications:

• Sensor Type: Back Illuminated, standard AR coated
• Active pixels (H x V): 512 x 512
• Pixel Size (H x V: µm): 16 x 16 μm
• Image Area: 8.2 x 8.2 mm with 100% Fill Factor
• Active Area Pixel Well Depth (e-): 80,000 180,000
• Pixel Readout Rate
• Minimum Temperature, air cooled, ambient 20°C: 10 MHz -70ºC, 17 MHz•3 -70ºC
• Chiller Liquid Cooling, coolant @ 10°C, >0.75l/min: 10 MHz -80ºC, 17 MHz•3 -80ºC
• Thermostatic Precision: ± 0.01°C
• Triggering: Internal, External, External Start, External Exposure, Software Trigger
• System Window Type: UV-grade fused silica, Broadband Visible-Near Infrared, 0.5 degree wedge
• Digitization: 16-bit (at all speeds)
• PC Interface: USB 2.0
• Lens Mount: C-mount
• Dark Current and Background Events•4,5:
• Dark Current (e- /pixel/sec) @ -80°C: 0.00030
• Spurious Background (events/pix) @ 1000x gain / -80°C: 0.0018
• Active Area Pixel Well Depth: 180,000 e
• Gain Register Pixel Well Depth•6: 800, 000 e
• Read noise (e-) •7: <1
• Linearity•8: Better than 99.9%
• Vertical Clock Speed: 0.3 to 3.3 µs (user selectable)
• Timestamp accuracy: 10 ns

The Leap technologies CTC PAL HTC Auto sampler for HPLCs offer Precise and Accurate sample Loading, high throughput, flexible analysis while taking up limited bench space.

Includes:

• HTC PAL Autosampler (Width: 534mm Depth: 385mm Height: 648mm)
• Temperature controlled sample storage 3 drawer
• XYZ injector/Arm
• LC Injection valve- 6 port
• Wash station
• Gameboy controller
• Power Supply
• Manual

Description:

FlowCam® is an imaging particle analysis system that uses flow microscopy to image and analyze subvisible particles with diameters ranging from 1 μm to 600 μm. Simultaneously determine particle shape, type, and size distribution of all detectable particles in your solution.

Specifications:

• Particle Size Range: 1 μm to 600 μm
• Magnification & FlowCells:
  • 20X (~200X magnification), flow cell depth option: 50 μm Field-of-View (FOV)
  • 10X (~100X magnification), flow cell depth option: 100 μm FOV
  • 4X (~40X magnification), flow cell depth option: 300 μm and 600 μm FOV
• Sample Processing Capability: 0.05 mL/minute at 20X and up to 3mL/minute at 4X
• Camera: High resolution (1920x1200 pixels) CMOS. Monochrome and color available.
• Frame Rate: Shutters up to 100 frames per second.
• Fluidics: Micro-syringe pump with multiple sizes to optimize flow rates: 0.5 mL, 1 mL, 5 mL

Description:

• Programmable pre-analysis sample preparation
• Heating & cooling features
• Self-contained system
• Vertical set-up saves bench space
• Standard SBS format labware
• Disposable pipette tips
• High accuracy fluid dispensing
• Complete control of workflows
• Particle identification via Microsoft Excel®

Specifications:

• Operating principle: Single robotic XYZ arm, hydraulic fill on all fluidics
• Labware format: SBS
• Number of positions for labware: 7
• Maximum number of wells per deck position: 96
• Maximum volume dispensed per operation: 1000μl
• Minimum volume dispensed during dilution or reagent operations: 5μl
• Minimum volume dispensed into FlowCam for analysis: 100μl
• CV of aspiration/dispensing accuracy: ±0.5%
• Pipette format: Disposable
• Environmental (particulate) control: Positive pressure HEPA filtration
• Sterilization: UV, programmable exposure time
• Mechanical orbital method: 100 -2000 RPM clockwise
• Sample conditioning: Heating/cooling 2-90°C
• Mixing: Aspiration/dispensing method
• Multiple assays/workflows supported: Yes
• Operation pause and resume: Yes
• CFR21 Compliant: Yes
• Workspace illumination: User controlled white LED
• Dimensions (H x W x D): 49 x 30 x 25” (124.5 x 76.2 x 63.5 cm)
• Weight: 190 lbs. (86.7 kg)
• Voltage: 110-220 V
• Power: 700 watts, including FlowCam
• Operating Temperature Operating: 5-40°C
• Storage Temperature: -20-60°C

Description:

Based on proven FlowCam DIPA technology, and optimized for larger particles (50μm to 5mm), FlowCam Macro provides rapid particle characterization that goes beyond just particle size. Direct, image-based measurements of particle size and shape enable differentiation of particle types in a heterogeneous mixture.

Specifications:

• Particle Count: Yes
• Concentration: Yes
• Particle Size (ESD): Yes
• Particle Size Range: 50μm to 5mm
• Technology: Dynamic Imaging Particle Analysis
• Image Type: 8-bit Grayscale (Monochrome Camera) or 24-bit Color (Color Camera)
• Image Format: 8-bit (monochrome) TIFF or 24-bit (color) TIFF
• Fluidics: High capacity industrial peristaltic pump, 2mm (deep) or 5mm (deep) flow cell
• Flow Rate: Up to 1 liter/minute

Description:

Introducing the FlowCam Nano® by Fluid Imaging Technologies. This patented oil immersion, flow imaging technology complements our industry-leading imaging particle analysis system to provide you with the most comprehensive particle analysis research and development tool for parenteral drug analysis.

Specifications:

• Method: Oil immersion flow microscopy
• Size Range: 300 nm to 30 μm
• Minimum Sample Volume: 20 μl
• Magnification & Flow Cell: 40X magnification with 50 μm flowcell
• Numerical Aperture (NA): 1.4 NA
• Camera’s Field of View: 150 μm height x 200 μm wide
• Camera Frame Rate: Up to 120 frames per second
• Focus Method: Manual
• Flow Rate: 0.02 mL/minute
• Image Format/Type: TIFF/ 8-bit Grayscale

Optimized sensitivity and selectivity, for reliable quantitative and qualitative information

The Agilent 1100 Series fluorescence detector provides high-sensitivity multisignal detection, online fluorescence spectra, straightforward Operation and maintenance, and built-in features for GLP compliance.

• Simplified optical design for optimized baseline stability.
• Long-life Xenon lamp for highest sensitivity.
• Variable-mode monochromators for selective multisignal detection and online spectra acquisition.
• Easy front access to flow cell for fast inspection or exchange.

Specifications

• Detection type:  Multisignal fluorescence detector with rapid online scanning capabilities and spectral data analysis
• Performance:  10 fg anthracene LOD, Ex 250 nm, Em 400 nm*, Raman (H20) >400 Ex 350 nm, Em 397 nm, dark value 450 nm, standard flow cell
• Light source: Xenon flash lamp, normal mode (20W) and economy mode (5W)
• Pulse frequency: 296 Hz for signal mode, 74 Hz for economy mode
• Excitation monochromator: Concave holographic grating, F/1.6, blaze 300 nm, range 200—700 nm and zero-order, bandwidth 20 nm
• Emission monochromator: Concave holographic grating, F/1.6, blaze 400 nm, range 280—900 nm and zero-order, bandwidth 20 nm
• Reference system: Inline excitation measurement
• Time programing: Up to 4 signals, response time, PMT gain, baseline behaviour (append, free, zero), spectral parameters
• Spectral acquisition: Excitation or emission spectra, scan speed 28 ms per datapoint, e.g. 0.6 s per spectrum 200—400 nm, 10 nm step
• Step size: 1—20 nm
• Spectra storage: Limited only by disk space
• Wavelength repeatability: ±0.2 nm
• Wavelength accuracy: ±3 nm
• Flow cells: Standard: 8 µl volume, 20 bar (2 MPa) pressure maximum, quartz
• Control and data evaluation: Agilent ChemStation for HPLC or Agilent 1100 Series control module with limited spectral data analysis and printing of spectra
• Analog outputs: Recorder/integrator: 100 mV or 1 V, output range >102 luminescence units, 2 outputs
• Communications: Controller-area network (CAN), HP-IB, RS-232C, LAN, APG Remote: ready, start, stop and shut-down signals
• Safety and maintenance: Extensive diagnostics, error detection and display (through control module and ChemStation), leak detection, safe leak handling, leak output signal for shutdown of pumping system. Low voltages in major maintenance areas.

The Agilent Technologies 1200 Series (G1310B) 1260 Infinity Isocratic Pump is a HPLC workhorse for demanding QA/QC and routine applications, designed to provide virtually pulse-free and stable solvent flow. This delivery principle allows variable stroke volume optimized to selected flow rate. The broadened pressure range with maximum pressure of 600 bar allows the use of small particle size columns, longer columns, or alternative solvents of higher viscosity. Additionally, the G1310B is the ideal pump for GPC/SEC applications where run-to-run and day-to-day precision of retention times are important.

Specifications:

• Settable flow range: set points 0.001 - 5 mL/min, in 0.001 mL/min increments
• Flow range: 0.2 to 10.0 mL/min
• Flow precision: ≤ 0.07 % RSD or ≤ 0.02 min SD
• Flow accuracy: ± 1 % or 10 μL/min whatever is greater, pumping degassed H2O at 10 MPa (100 bar)
• Pressure operating range:
  • Operating range up to 60 MPa (600 bar, 8700 psi) up to 5 mL/min
  • Operating range up to 20 MPa (200 bar, 2950 psi) up to 10 mL/min