Used Lab Equipment

The GS-800 calibrated densitometer offers superior accuracy, sensitivity, and data reproducibility. It automatically self-calibrates the optical density to optimize detection.Unlike laser densitometer systems, the GS-800 calibrated densitometer has both transmittance and true reflectance capabilities that allow accurate scans of both transparent and opaque samples. The advantage of true reflectance scanning is accurate detection and analysis of molecules on the surface of membranes, rather than molecules distributed throughout the membrane matrix.

Specifications:

• Wavelength: 400-750 nm
• Absorbance Range:0 - 3 OD (transmissive), 0 - 2 OD (reflective)
• Scanning Area: 29 x 40 cm (transmissive), 30 x 40 cm (reflective)
• Sampling Rate:800 x 1600 dpi
• Pixel Density:12 bit
• Dimensions: 3 x 62.6 x 19.3 cm
• Weight:8 kg

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

• Complete protein transfer in 7 minutes or less
• High detection sensitivity and even transfer
• Increased blotting reliability and reproducibility
• Flexible gel size formats and membrane types
• A simple, user-friendly system
• Options to create new custom programs
• Built-in tutorial and application notes
• High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

• For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
• Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
• Allows users to create custom programs

iBlot 2 Transfers Save Time

• With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
• The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

• Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
• The high density of ions in the gel matrix enables rapid protein transfer
• During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
• Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
• Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

Key Features:

• The ultimate Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) system provides the highest quality data
• Powerful Voyager Version 5 with Data Explorer software for flexible acquisition and data processing
• Reflectron provides high mass accuracy and post-source decay (PSD)
• Fully automated acquisition and processing of data provides higher throughput