Used Lab Equipment

Includes:

• Computer w/ GenePix Pro -  MicroArray Acquisition & Analysis Software
• Flat Panel Monitor
• All Necessary Cords, Cables
• Manual

Features:

• Speed. Unlike most commercially available microarray scanners, the GenePix 4000B Microarray Scanner acquires data at two wavelengths simultaneously. This means drastically reduced scan times and hence greater workflow efficiency. Apart from superior speed, simultaneous scanning eliminates potential errors associated with aligning two separately scanned images, and provides real-time access to data as they are acquired.
• Add Reliability to Your Research. The performance calibration standards that come with your GenePix 4000B Microarray Scanner allow you to check the performance when you receive your scanner and at any time in the future. GenePix Pro Acquisition and Analysis Software includes one-touch calibration to restore any GenePix scanner to benchmark performance. You can rest assured knowing the signals you generate today are the same as a couple of years ago and in the future.
• Automate Your Workflow. With automated photomultiplier (PMT) balancing. The fully integrated GenePix Pro Acquisition and Analysis Software, controls the GenePix 4000B Microarray Scanner and can automatically evaluate the required PMT gain values, for fast and easy optimization of signal intensity and channel balance. The GenePix 4000B Microarray Scanner then acquires data at your selected resolution between 5-100 micron, allowing the adjustment of image resolution and file size for each experiment.
• Get More Published. For over 10 years and close to 6000 literature references, the GenePix 4000B Microarray Scanner has expanded the overall sample compatibility, with user-adjustable focus offset and laser power settings. This allows proper imaging of slides with either a raised surface, as with membrane-coated glass, or a recessed surface, as in embedded arrays.

Performance Specifications

• Sample type: Standard microscope slide (1" x 3", 25 x 75 mm or 26 x 76 mm)
• Scan area: Adjustable, 22 x 71.5 mm max.
• Excitation: 532 nm and 635 nm solid-state lasers
• Laser power settings: User-selectable 100%, 33% or 10% laser power
• Emission filters: Two, optimized for Cy3 and Cy5 or spectrally similar dyes
• Detection: Dual photomultipliers (PMTs), automatic and manual gain adjustment
• Focus offset: Adjustable between -50 and +200 μm (5 μm increments)
• Optics: Non-confocal
• Scanning method: Simultaneous, 2-channel
• Scan time:5 minutes for both channels, 10 μm resolution, full scan area
• Pixel resolution: Adjustable from 5-100 μm
• Digital resolution: 16-bit
• Dynamic range: Four orders of magnitude at SNR > 3
• Image type: Single- or multi-image TIFF
• Barcode reading: Image-based software barcode reader

General Specifications

• Dimensions (in.):5 (W) x 8.0 (H) x 17.5 (D)
• Dimensions (cm): 34 (W) x 20 (H) x 44 (D)
• Weight: 45 lbs. (20 kg)
• Power supply: 110/220V universal
• Genomics: Directed at the genome sequence itself, microarrays have been used to identify novel genes, binding sites of transcription factors, changes in DNA copy number, and variations from a baseline sequence, such as in emerging strains of pathogens or complex mutations in disease-causing human genes.

The SureScan Microarray Scanner is a laser induced fluorescence scanner designed to read microarrays printed on a standard 1 inch x 3 inch glass slides. It measures the fluorescence intensity of two dyes simultaneously from labeled sample nucleic acid bound to microarrays.

Features:

• 24 slide cassette allows for continuous loading during operation
• Automatic loading of raw data files into Feature Extraction software
• Built-in ozone protection
• Manufactured under ISO 13485 certification

Specifications:

• Dynamic Range
  • 104 (16-bit data format)
  • 105 (20-bit data format)
  • 106 (with XDR scanning)
• Dynamic AutoFocus: Continually adjusts scanner's focus, keeping features in focus at all times
• Resolution: 2, 3, 5, and 10 microns
• Autoloader: 24-slide cassette allows for hands-off operation
• Integrated Barcode Reader: Reads code 128 (A,B,C), Code 39, Code 93, and CODABAR
• Compatible Dyes: Cyanine 3 and Cyanine 5, and Alexa 647, 555, and 660
• Laser Information:
  • Green solid-state laser, 532 nm;
  • red solid-state laser, 640 nm
• Power: 20 mW at 532 nm and 640 nm both controlled to 13 mW
• Scan Window Maximum: 71 mm x 21.6 mm
• PMT Adjustment Automatic PMT gain calibration before each run
• Allows adjustment of signal levels from 100% (default) to 1%
• Detection Limit 01 chromophores per square micron
• Pixel Placement Error 1 pixel @ 5 micron resolution
• Uniformity: 5% CV global non-uniformity; average local non-uniformity is typically 1% based on 100 micron features
• Glass Dimensions Supported: 1 x 3" (25 mm x 75 mm) glass slide
  • Width: 24.95 mm to 26.1 mm
  • Length: 74.8 mm to 76.45 mm
  • Thickness: 0.9 mm to 1.1mm
  • Non-Mirrored slides of low intrinsic fluorescence
• Scan time: Two-color simultaneous data acquisition in 16 minutes per for 3-micron scans and 24 minutes for 2-micron scans (scan region of 61 mm x 21.6 mm)
• Data Workstation and Operating System: PC-based with Windows® 7 – 64 bit
• Approximate Scanner Dimensions: Height: 16.5” (42 cm), Width: 17” (43 cm); Depth: 26” (67 cm)
• Weight Scanner: 125 lbs (56.8 kg)
• Power Input: 100 – 240 VAC, 50 - 60 Hz, 250 VA max.
• Fuses: Two power supply fuses: T4A, 250 VAC
• Humidity: Operating: 15% to 85% RH at 30°C
• Altitude: Operating maximum: 2,300 m (7,500 ft)
• Operating Temperature Range: 15°C to 30°C
• Laser Product Classification: Class 1

Specifications:

Performance:

• Restacking feature allows plates to be returned to the input stack in their original order
• Can be operated without an external computer when used with the ELx405 Microplate Washer, MicroFill Microplate Dispenser, NanoQuot Microplate Dispenser, or MicroFlo Select Microplate Dispenser
• 30 or 50 standard height microplates (96- or 384-well) per interchangeable stack Optional rotational wrist allows users to integrate both landscape and portrait orientation microplate carriers with the instrument remaining in its optimal, ergonomic-friendly position
• Variable microplate input Small footprint conserves precious benchtop space; fits easily in most standard laboratory hoods

Design:

• Universal design compatible with most supported BioTek instrumentation Designed for heavy use with aluminum precision-ground base Linear mechanisms built around proven BioTek technology Quiet operation with cams activated by stepper motors No moving parts to wear within motors Gripper incorporates rack and pinion drive for synchronistic movement Reliable transport with microplate gripped from below Onboard self-diagnostics ensure trouble-free operation

Features:

• <10 seconds plate exchange time
• Range of formats including 24-, 48-, 96-, 384- and 1536-well
• 10, 30, or 50 standard height microplates per interchangeable stack
• De-lidding and re-lidding capability with patented BioStack 4
• Rotational gripper for ergonomic  and compact integration
• Restacking capability
• Optional barcode scanner
• Direct control through washer and dispenser interface or via LHC or Gen5 PC software
• Small footprint conserves precious bench top space
• Walk away automation

Features:

• Olympus BX61 Trinocular Frame
• (2) 10X/22 EyePieces
• 6 Position Fluorescence Illuminator
• Trinocular Head
• Applied Scientific MS-2000 X,Y,Z Motorized Stage
• MS-200 Controller
• Olympus BX-UCB and Controller for Motorized Nosepiece
• 100mg Fluorescent light w/ power supply OR Xcite 120Q Metal Halide OR Xcite 120LED Mini or Boost
• Objectives
  • UPlanSApo 4x
  • UPlanSApo 10X
  • UPlanSApo 20X
  • UPlanSApo 40X with focus adjustment ring
  • PlanApo N 60X Oil Immersion
• Filter Cubes
  • Rhodomine
  • FITC
  • Dapi

The Incu-Mixer MP heated microplate mixer is designed to provide an optimal method for vortexing and incubating microplates. Simultaneous heating from both the bottom surface and the lid provide temperature uniformity throughout the sample. Additionally, the heated lid minimizes evaporation and prevents condensation. A compact, 2mm orbit ensures thorough mixing in microplates without splashing. Speed, time and temperature are all displayed in real time on the large LCD screen while an internal validation system constantly monitors all parameters for accuracy. Designed for application flexibility, the Incu-Mixer series is available in two, and four-plate capacities, both capable of accepting all standard plates up to 40mm high. Independent time, temperature and speed controls allow the mixer to function as 3 machines in one; a multi-plate incubator, vortexer, or a combination of the two. The Incu-Mixer MP series is ideal for a wide variety of molecular biology applications including immunochemical reactions, enzyme and protein analysis, and microarray analysis.

Specifications:

• Temp. Range: Ambient +5 to 70°C
• Orbit: 2mm

• Mixing Speed:

• 100 to 1500rpm (4-Plate)
• 100 to 1200rpm (2-Plate)
• Temp. Increment: 0.1°C
• Temp. Accuracy: +/- 0.2C
• Temp. Uniformity: +/- 0.5C
• Speed Increment: 10 rpm

• Dimensions (W x D x H):

• 12.6 x 13.8 x 7.3 in. / 32 x 35 x 18.5 cm (4-Plate)
• 11 x 10.6 x 5.5 in. / 28 x 27 x 14 cm (2-Plate)
• Weight: 18 lbs (8kg)
• Timer: 1 min. to 99 hours 99min. / continuous

• Electrical:

• 115 or 230V, 50-60Hz, 600W (4-Plate)
• 100 to 240V, 50-60Hz, 500W (2-Plate)

The Incu-Mixer MP heated microplate mixer is is designed to provide an optimal method for vortexing and incubating microplates. Simultaneous heating from both the bottom surface and the lid provide temperature uniformity throughout the sample. Additionally, the heated lid minimizes evaporation and prevents condensation. A compact, 2mm orbit ensures thorough mixing in microplates without splashing. Speed, time and temperature are all displayed in real time on the large LCD screen while an internal validation system constantly monitors all parameters for accuracy. Designed for application flexibility, the Incu-Mixer series is available in two, and four-plate capacities, both capable of accepting all standard plates up to 40mm high. Independent time, temperature and speed controls allow the mixer to function as 3 machines in one; a multi-plate incubator, vortexer, or a combination of the two. The Incu-Mixer MP series is ideal for a wide variety of molecular biology applications including immunochemical reactions, enzyme and protein analysis, and microarray analysis.

Specifications:

• Temp. Range: Ambient +5 to 70°C
• Orbit: 2mm

• Mixing Speed:

• 100 to 1500rpm (4-Plate)
• 100 to 1200rpm (2-Plate)
• Temp. Increment: 0.1°C
• Temp. Accuracy: +/- 0.2C
• Temp. Uniformity: +/- 0.5C
• Speed Increment: 10 rpm

• Dimensions (W x D x H):

• 12.6 x 13.8 x 7.3 in. / 32 x 35 x 18.5 cm (4-Plate)
• 11 x 10.6 x 5.5 in. / 28 x 27 x 14 cm (2-Plate)
• Weight: 18 lbs (8kg)
• Timer: 1 min. to 99 hours 99min. / continuous

• Electrical:

• 115 or 230V, 50-60Hz, 600W (4-Plate)
• 100 to 240V, 50-60Hz, 500W (2-Plate)

The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. Offering unmatched flexibility and reliability, the systems are ideal for a wide variety of applications and chemistries (including but not limited to: gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis and chromatin immune-precipitation studies.

Highly reproducible results are the product of the Mx3005P’s single light source, single detector precision optic scanning design, providing uniform excitation and detection, coupled with the trusted Peltier-based thermal system, which ensures uniform ramping and thermal accuracy.

Features:

• Computer w/ MxPro QPCR Software
• Four channels with customizable filters for multiplex up to 4 simultaneous targets
• Open platform supports all fluorescent chemistries
• 96 Well Block

Instrument Specifications

• Excitation source: Quartz Tungsten Halogen lamp
• Detector: 1 scanning photomultiplier tube (PMT)
• Multiplexing capabilities: 5 targets in the same tube
• Thermal system: solid-state, Peltier-based
• Embedded computer safeguards data
• Tube/plate formats: 200 µL , 96-well format, optimized for 25 µL sample size
• Filters: Dual customizable filter wheels with four user-selected filters*
• Dynamic Range: 10 orders of magnitude
• Excitation Range: 350nm-750nm
• Emission Range: 350nm-700nm
• Temperature Uniformity: +/- .25°C at 72ËšC
• Temperature Ramp Rate: up to 2.5°C/second
• Temperature Range: 25-99°C
• Dimensions: 14" (35.6 cm) W x 20" (50.8 cm) D x 18" (45.7 cm) H, weight: 45 lbs (20.4 kg)
• Standard Filters
  • FAM/SYBR Green 1
  • HEX/JOE/VIC
  • ROX/Texas Red
  • Cy5
  • Cy3

Includes:

• Computer
• Software
• All Necessary Cables
• Manuals

Quantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables.

The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses.

The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction.

Applications

• DNA target quantification
• Gene expression
• SNP detection, Allele discrimination, GenotypingDNA methylation, Apoptosis
• Viral load assays, Pathogen detection
• Clinical diagnosis (Cancer therapy response)

Features:

Enhance the limit of detection for DNA, RNA and protein with the Thermo Scientific NanoDrop 2000c UV-Vis spectrophotometer featuring both microvolume pedestal and cuvette measurement options. The cuvette station is ideal for measuring dilute nucleic acid and protein assays, includes heating and microstirring capabilities, and allows users to perform kinetics and cell culture readings (600nm).

• Wide spectral range (190-840nm) for measuring a variety of samples types:
  • Peptides (205nm)
  • DNA and RNA (260nm)
  • Purified protein (280nm)
  • Toxicology assays and industrial dyes (490nm)
  • Gold nanoparticles (520nm)
  • Colorimetric protein assays (BCA 562nm, Bradford 595nm, Modified Lowry 650nm, Pierce 660 660nm)
  • Optical Density measurements (600nm)

Includes:
• Computer w/ Software
• Necessary Cables
• Manual

The NanoDrop 1000 Spectrophotometer measures 1 ul samples with high accuracy and reproducibility. The full spectrum (220nm-750nm) spectrophotometer utilizes a patented sample retention technology that employs surface tension alone to hold the sample in place. This eliminates the need for cumbersome cuvettes and other sample containment devices and allows for clean up in seconds. In addition, the NanoDrop 1000 Spectrophotometer has the capability to measure highly concentrated samples without dilution (50X higher concentration than the samples measured by a standard cuvette spectrophotometer).

UV/VIS spectrophotometry is simple for samples as small as 1 ul using the NanoDrop 1000 Spectrophotometer. The small sample requirement and ease of use make the NanoDrop 1000 Spectrophotometer ideally suited for measuring:
• Nucleic acid concentration and purity of nucleic acid samples up to 3700 ng/ul (dsDNA) without dilution
• Fluorescent dye labeling density of nucleic acid microarray samples
• Purified protein analysis (A280) up to 100 mg/ml (BSA)
• Expanded spectrum measurement and quantitation of fluorescent dye labeled proteins, conjugates, and metalloproteins
• Bradford Assay analysis of protein
• BCA Assay analysis of protein
• Lowry Assay analysis of protein
• Pierce Protein 660 nm Protein Assay
• Cell density measurements
• General UV-Vis spectrophotometry

Specifications:

Sample Size: 1 microliter

Path Length: 1 mm (with auto-ranging to 0.2 mm)

Light Source: Xenon flash lamp

Detector Type: 2048-element linear silicon CCD array

Wavelength Range: 220-750 nm

Wavelength Accuracy: 1 nm

Wavelength Resolution: 3 nm (FWHM at Hg 546 nm)

Absorbance Precision: 0.003 absorbance ( 1mm path)

Absorbance Accuracy: 2% (at 0.76 absorbance at 257 nm)

Absorbance Range: 0.02-75 (10 mm equivalent absorbance)

Detection Limit: 2 ng/microliter (dsDNA)

Maximum Concentration: 3700 ng/microliter (dsDNA)

Measurement Cycle Time: 10 seconds

Dimensions: 14 cm X 20 cm

Weight: 1.6 kg

Sample Pedestal Material of Construction: 303 stainless steel and quartz fiber

Operating Voltage: 12 Vdc

Operating Power Consumption: 6 W

Standby Power Consumption: 1.5 W Heating : 37 ± 0.5 °C

Includes Computer Loaded w/Control Software

The Typhoon FLA 7000 is a fast and versatile laser scanner for biomolecular imaging applications including sensitive and quantitative measurements of radioisotopic labels by storage phosphor and single fluorescence, as well as digitization of colorimetric stains (e.g., Coomassie™ Blue and silver-stained gels). Typhoon FLA 7000 IP is a model dedicated to phosphor imaging. It comes with only one laser configuration. Upgrade paths for any of blue, green, and red excited fluorescence imaging are provided.  

Features:

• Versatility: The system images radioisotopic, visible fluorescent, chemifluorescent, and colorimetric samples.
• High resolution and quantitation: 16-bit images are generated at up to 25 μm pixel resolution. A linear signal response over a span of five orders of magnitude gives precise quantitation in gels, blots, and tissue sections.
• High speed: a 24 × 25 cm gel can be scanned in less than two minutes at 100 μm resolution without compromising sensitivity
• High sample throughput: a scanning area of 24 × 40 cm enables simultaneous imaging of up to 12 gels or blots, measuring 10 × 8 cm in size. This facilitates comparisons among blots, and reduces workload and waiting time.

Specifications:

• Imaging modes: Fluorescence, phosphorimaging, and digitization
• Excitation wavelengths:
  • 473 nm (blue LD laser),
  • 532 nm (green SHG laser)
  • 635 nm (red LD laser)
  • 650 nm (red LD laser)
• Dynamic range: 5 orders of magnitude
• Bit depth: 16-bit
• Max scanning area:
  • Phosphorimaging: 20 × 40 cm
  • Fluorescence: 24 × 40 cm
• Pixel sizes: 25, 50, 100, and 200 μm
• Standard filters: IP (phosphorimaging), Y520, O580, R670
• Dimensions (W × H × D): 940 × 360 × 556 mm
• Weight: 62 kg
• Line frequency: 50 to 60 Hz
• Temperature: 15°C to 30°C
• Humidity: 30% to 70% (no condensation)
• Supply voltage: 100 - 240 VAC ± 10%
• Power consumption: Approx. 0.3 kVA

The Typhoon instrument is a variable-mode imager that produces digital images of radioactive, fluorescent, or chemiluminescent samples. The Typhoon instrument is available in several models. The Typhoon Trio instrument contains three internal lasers (blue, green, and red).

Includes:

• Red, Blue, Green Laser Modules Built in
• Image Quant and Scan Control Software loaded on computer
• All Necessary Cables, Cords and Manuals

For DNA, RNA, and protein samples, users can choose from:

• Storage phosphor autoradiography
• Direct green (532 nm) excited fluorescence
• Direct red (633 nm) excited fluorescence
• Direct blue (488 nm) excited fluorescence
• Chemiluminescence

Features:

• When one of the five scanning modes is selected, the appropriate optical components are automatically activated.
• Typhoon imagers scan mounted and unmounted storage phosphor screens, gels and blots up to 35 x 43 cm as well as microarrays.
• Typhoon imagers exhibit outstanding linearity and quantitative  accuracy, and include ImageQuant TL Image Analysis Software.
• Typhoon imagers are fully optimized as part of the Ettan DIGE system, and seamless integration with DeCyder Differential Analysis Software
• is ensured for all models.
• Special tray templates that are part of the latest scanner control software and new optional Gel Alignment Guides add higher throughput and ease of handling 2-D gels run on Ettan DALT and SE600.

The Typhoon instrument is a variable-mode imager that produces digital images of radioactive, fluorescent, or chemiluminescent samples. The Typhoon instrument is available in several models. The Typhoon Trio instrument contains three internal lasers (blue, green, and red).

Includes:

• Red, Blue, Green Laser Modules Built in
• Image Quant and Scan Control Software loaded on computer
• All Necessary Cables, Cords and Manuals

For DNA, RNA, and protein samples, users can choose from:

• Storage phosphor autoradiography
• Direct green (532 nm) excited fluorescence
• Direct red (633 nm) excited fluorescence
• Direct blue (488 nm) excited fluorescence
• Chemiluminescence

Features:

• When one of the five scanning modes is selected, the appropriate optical components are automatically activated.
• Typhoon imagers scan mounted and unmounted storage phosphor screens, gels and blots up to 35 x 43 cm as well as microarrays.
• Typhoon imagers exhibit outstanding linearity and quantitative  accuracy, and include ImageQuant TL Image Analysis Software.
• Typhoon imagers are fully optimized as part of the Ettan DIGE system, and seamless integration with DeCyder Differential Analysis Software
• is ensured for all models.
• Special tray templates that are part of the latest scanner control software and new optional Gel Alignment Guides add higher throughput and ease of handling 2-D gels run on Ettan DALT and SE600.