Used Lab Equipment

The EMax Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. A staggering mean time between failure (MTBF) of 20,000 hours is achieved by 97 independent and stationary fiber optics so that the microplate remains stationary during the entire reading cycle, reducing the number of moving parts. No optical alignment needed during the lifetime of the instrument!

Includes:

• Computer Loaded with SoftMax Pro 5.4.1
• All Necessary Cables and Cords
• Manuals

Features:

• Extend uptime with an estimated 20,000 hour mean time between failures
• Maximize assay signal-to-noise ratios through a unique optical system that directs all available light to each well
• Achieve higher throughput with a 5 second microplate read time
• Read up to 2 wavelengths in endpoint absorbance for 96-well plates
• Mix samples in microplate wells by user-programmable Automix feature
• Operate without an attached computer using the onboard microprocessor
• Add assay flexibility with additional filters

General Specifications:

• Dimensions (H x W x D):4 x 18.3 x 13.4 in.
• Wavelength range: 400 - 750 nm
• Photometric range:000 to 4.000 OD
• Wavelength selection: Filters
• Photometric resolution: 0.001 OD
• Endpoint read time: 5 seconds
• Temperature range: Ambient

Specifications:

• Optical System: 12 measurement channels, 1 reference channel
• Wavelength Range: 340 to 750nm
• Wavelength selection: Interefence filters (slide of 4 filters) Bandwidth:10nm
• Measurement Range: 340 to 399nm: 0 to 3.0 OD, 400 to 750 nm: 0 to 4.0 OD
• Accuracy: 0.0 to 2.0 OD/492 nm
• 4 Filter Optics

The ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. The Microsoft Windows-based software guarantees smooth operation as it includes single and dual wavelength read, kinetic readings, automix (shake), endpoints, blank patterns, as well as curve fits. The ThermoMax microplate reader provides thermal control and vertical shake, perfect for kinetic readings involving growth curves at 5 seconds per plate. The system can perform readings in a photometric analysis mode as well as kinetic analysis at user-configurable kinetic ranges and intervals, run on-board and temperature regulation diagnostics.

Features:

• Wide photometric range
• Temperature-regulated
• Thermal control and vertical shake
• Microsoft Windows-based software
• Automatic mixing
• Five-second read per plate
• Photometric and kinetic analysis mode

Specifications:

• Wavelength range:- 340-750 nm
• Photometric range: 0.000-4.000 OD
• Kinetic read time: 5 second minimum between readings.
• Endpoint read time: 6 sec.
• Modes: Single wavelength, Dual wavelength, Kinetics, Range, Blank pattern.

The SmartReader 96 comes complete with 4 popular filters: 405,450,492,630nm and twenty six additional filters are available separately to cover a full range from 340 to 750nm.  The integrated filter wheel allows up to 8 filters to be loaded at once, and filters can be selected during programming. Up to two filters can be used for 2 measurements of the sample plate, for calculated comparisons.  Kinetic calculations can be performed by setting multiple readings. The SmartReader 96 is a convenient stand alone reader, all software and the programing system is incorporated into the electronics. However, a convenient USB port on the side allows quick and easy saving of data files to a USB drive, and the universal .csv files can then be shared or transferred to a computer for storage or further processing.  A data port can be used for connecting a computer or basic printer.

Specification:

• Control and Display: 7 inch touch screen (800 x 480 pixels)
• Light Source: Quartz-halogen lamp 6V/10W
• Wavelength Range: 340-750nm (405, 450, 492 and 630 nm filters included)
• Half-Bandwidth of filter: 3~9nm
• Readout Range: 0~4.000 Abs
• Linearity (405nm): 0~2.000 Abs <+1% / 0~4.000 Abs <+2%
• Precision: CV</= 0.2% (0-3) Abs ; CV</= 01.0% (3-4) Abs
• Test Speed: <6 sec. for 96 well plate
• Shaking: 3 modes - slow, medium, fast
• User Interface: Integrated software and touch screen control
• Storage: 200 programs; 100,000 test records
• Ports: USB ports for PC, printer, mouse and flash drive
• Dimensions: 295x440x225 mm (11.6 x 17 x 8.75 inches)
• Weight: 10 kg

Description:

FlowCam® is an imaging particle analysis system that uses flow microscopy to image and analyze subvisible particles with diameters ranging from 1 μm to 600 μm. Simultaneously determine particle shape, type, and size distribution of all detectable particles in your solution.

Specifications:

• Particle Size Range: 1 μm to 600 μm
• Magnification & FlowCells:
  • 20X (~200X magnification), flow cell depth option: 50 μm Field-of-View (FOV)
  • 10X (~100X magnification), flow cell depth option: 100 μm FOV
  • 4X (~40X magnification), flow cell depth option: 300 μm and 600 μm FOV
• Sample Processing Capability: 0.05 mL/minute at 20X and up to 3mL/minute at 4X
• Camera: High resolution (1920x1200 pixels) CMOS. Monochrome and color available.
• Frame Rate: Shutters up to 100 frames per second.
• Fluidics: Micro-syringe pump with multiple sizes to optimize flow rates: 0.5 mL, 1 mL, 5 mL

Features:

PD2100 Series Detector

Description:

The Dynamax UV-DII absorbance detector measures absorbance alternately at first and second wavelengths. When a measurement is made at one wavelength, a projection is made, based on past measurement, of the value of absorbance at the other wavelength. A ratio is taken of the measured value at one wavelength with the projected absorbance at the other wavelength

Specifications:

• Power: 100V/120V/220V/240V
• Hz: 50-60Hz
• Fuse: 5ASB/100/120V, 1.25ASB/220/240V

Features:

The Bio-Plex 3D suspension array system is the next generation multiplexing platform based on xMAP technology. Expanded multiplexing capability, faster time to results, and automation capability make it the platform of choice for high-throughput testing for nucleic acid and protein applications.

Specifications:

• Software: xPONENT (acquisition) Bio-Plex Manager (analysis)
• Optics: Lasers/APDs/ PMTs
• Multiplex Capacity: 500
• Bead Compatibility: MagPlex-C, MicroPlex, LumAvidin, xTAG
• Read Time: ~20 min/96-well plate, ~75 min/384-well plate
• Applications: High-throughput
• Plate Format: 96-well/384-well
• Dynamic Range:5 logs

Includes: computer, touch screen monitor, manuals, and software.

Includes:

• Bar Code reader
• Computer loaded with Software

The 2103 EnVision Multilabel Plate Readers are fast, sensitive and versatile benchtop readers that deliver optimized performance in every application and for every label. Their unique design features modular label-specific optical mirror modules, high energy flash lamps, and high speed detectors.

Specifications:

• Light Source: Xenon flash lamp
• Plate Formats: 1 to 3456
• Temperature Control:
  • Temperature range: from 2 °C above ambient up to 45 °C (0.5 °C increments)
  • Uniformity: ± 1 °C
  • Heating time: < 10 minutes (from RT up to 37 °C)
• Shaking: linear, orbital, dual orbital
• Dispenser:
  • Dispense volume: 2-475 μL (1 μL increments)
  • Pump speed: 100-500 μL/s
  • Dead volume: 750 μL. With pump-back function < 50 μL using stardard tubing length and factory settings.

Includes:

• Bar Code reader
• Computer loaded with Software

The 2103 EnVision Multilabel Plate Readers are fast, sensitive and versatile benchtop readers that deliver optimized performance in every application and for every label. Their unique design features modular label-specific optical mirror modules, high energy flash lamps, and high speed detectors.

Specifications:

• Light Source: Xenon flash lamp
• Plate Formats: 1 to 3456
• Temperature Control:
  • Temperature range: from 2 °C above ambient up to 45 °C (0.5 °C increments)
  • Uniformity: ± 1 °C
  • Heating time: < 10 minutes (from RT up to 37 °C)
• Shaking: linear, orbital, dual orbital
• Dispenser:
  • Dispense volume: 2-475 μL (1 μL increments)
  • Pump speed: 100-500 μL/s
  • Dead volume: 750 μL. With pump-back function < 50 μL using stardard tubing length and factory settings.

The FilterMax F5 Multi-Mode Microplate Reader offerS a wide set of features at an affordable price-to-performance ratio. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements.

Specifications:

• Read modes:
  • Absorbance
  • Fluorescence (top/bottom read)
  • Luminescence (top read)
  • Time-Resolved Fluorescence (top read)
  • Fluorescence Polarization (top read)
• Wavelength ranges:
  • Abs: 230 - 650 nm
  • FL Ex: 230 - 650 nm 
  • FL Em: 390 - 700 nm 
  • Lumi: 400 - 700 nm
  • TRF: 340 - 700 nm
  • FP Ex: 300 - 650 nm
  • FP Em: 390 - 700 nm
• Wavelength Selection: Filters
• Absorbance photometric accuracy/linearity: 
  • <±0.5% and ±0.005 OD at 2.0 OD (400 nm to 650 nm)
  • <±0.5% and ±0.005 OD at 1.0 OD (230 nm to 399 nm)
• Absorbance photometric precision/repeatability: 
  • <±0.5% and ±0.005 OD at 2.0 OD (400 nm to 650 nm)
  • <±0.5% and ±0.005 OD at 1.0 OD (230 nm to 399 nm)
• Sensitivity top read optimized: 
  • FL: 0.3 fmol/well in 200 μL fluorescein 96 well (1.5 pM) , 0.1 fmol/well in 75 μL fluorescein 384 well (1.3 pM)
  • Lumi: 2 fmol/well in 200 μL ATP 96 well (10 pM), 0.75 fmol/well in 50 μL ATP 384 well (15 pM) 
  • TRF: 30 amol/well in 200 μL Europium 96 well (0.15 pM), 15 amol/well in 100 μL Europium 384 well (0.15 pM) 
  • FP: 1 mP at 10 nM fluorescein 96 well, 1.5 mP at 10 nM fluorescein 384 well
• Light source: 
  • High power LEDs
  • Deuterium lamp
• Detector: 
  • Silicon photodiode
  • Photomutiplier tube
• Read types:
  • Endpoint
  • Kinetic
  • Well scan
• Read times (kinetic interval for 96-well plate):
  • Abs: 27 seconds
  • FL: 29 seconds
  • Lumi: 28 seconds
  • FP: 54 seconds
• Plate shaking:
  • Linear
  • Orbital
• Plate type: 6-1536 well plates
• Temperature control: Ambient + 4°C to 45°C
• Dimensions: H 24 cm x W 39 cm x D 58 cm
• Validation tools: 
  • Multi-mode validation plate
  • SoftMax Pro GxP Software

Multiskan Spectrum makes getting results easier, replacing separate spectrophotometers and microplate reading instruments by providing both functions in one convenient, compact instrument.  The Multiskan Spectrum is truly two instruments in one, combining both cuvette reading capabilities and advanced microplate reading in one convenient, compact instrument.  You can easily take quick absorbance measurements in a cuvette between the cycles of a microplate enzyme kinetics experiment. 

For maximum flexibility, Multiskan Spectrum accepts all microplate for mats from 6- to 384-wells, as well as cuvettes from standard to ultra micro capacities in glass, plastic, or quartz. You can take multiple photometric readings per well on large format plates, or single measure ments requiring just 30 μl in 384-well plates.

Scanning range of 200 – 1000 nm.  The built-in monochromator makes it simple to select any wavelength from 200 nm to 1000 nm in 1 nm steps without ever changing a filter. Sample absorbances can be scanned over the whole spectrum either in microplates or cuvettes.

Typical applications of the Multiskan Spectrum

• UV/Visible spectrophotometry
• Spectrum scan
• Nucleic acid analysis
• RNA concentration (A260)
• ssDNA concentration (A260)
• dsDNA concentration (A260)
• DNA purity (A260/A280)
• Enzyme kinetics
• E.g.,Vmax and Km
• Protein analysis
• Biuret protein determination
• Bradford protein determination
• BCA protein determination

Technical Specifications

Performance

• Wavelength range 200 – 1000 nm
• Read-out range 0 – 4 Abs
• Linearity 0 – 3 Abs, ±/- 2% at 450 nm
• Accuracy ± 1% or ± 0.005 Abs (0 – 2 Abs)
• ± 2.0% (2 – 3 Abs)
• Precision CV < 1% (0 – 2 Abs)
• CV < 2% (2 – 3 Abs)
• Optical bandpass 2 nm
• Wavelength accuracy ± 1 nm
• Stray light < 0.02% at 230 nm

General Specifications

• Microplate types 6-, 12-, 24-, 48-, 96- and 384-well plates
• Wavelength selection Tunable monochromator, 200 – 1000 nm in increments of 1 nm
• Light source 10 W Xenon flash lamp
• Detectors Photodiodes
• Incubator temperature range Ambient (+25°C) +4°C to 45°C
• Mains input 100 – 240 V (50/60 Hz) autoranging
• Power consumption 200 VA
• Dimensions 397 mm (W) x 217 mm (H) x 450 mm (D)
• [15.6” (W) x 8.5” (H) x 17.7” (D)]
• Weight 12.5 kg (27.5 lbs.)

Includes:

• Computer Loaded with SoftMax Pro 5.4.1
• All Necessary Cables and Cords
• Manuals

The SPECTRAmax 250 microplate spectrophotometer has a xenon flash lamp and grating monochromator that facilitate direct UV measurement of DNA- RNA- and protein in microplates. Up to 96 assays can be run at one time using disposable or quartz microplates. The purity of DNA can be measured with A260/A280 ratios. Also- users can perform spectral scans from 250 nm to 750 nm to simplify qualitative analyses of numerous samples. The product reportedly reduces sample volumes in experiments because the assays are performed in a microplate instead of cuvettes. SOFTmax PRO microplate data- analysis software- for use with the SPECTRAmax 250- provides built-in spreadsheet capabilities for easy data analysis and reporting.

Specifications:

• Wavelength Range: 250-700 nm
• Photometric Range: 0.000 to 4.000 OD
• Wavelength Selection: Monochromator, tunable in 1-nm increments
• Photometric Linearity (405 nm): 0 to 3.000 OD
• Wavelength Bandwidth: 5 nm
• OD Resolution: 0.001 OD
• Endpoint Read Time: 9 seconds
• Temperature Range: Ambient to 45 C
• Kinetic Read Time: 9-second minimum interval
• Temperature Uniformity (Microplate): +/-0.5 C @ 37 C well-to-well

Includes:

• Computer Loaded with Softmax Pro 5.4.1
• All Necessary Cables and Cords
• Manuals

General Specifications

• Dimensions (in.): 8.6 (h) x 22.8 (w) x 15 (d)

Performance Specifications

• Wavelength Range: 340 - 850 nm
• Wavelength Selection: Monochromator, tunable 1.0 nm increments
• Wavelength Bandwidth: 2 nm
• Wavelength Accuracy: < ± 1.0 nm
• Wavelength Repeatability: ± 0.2 nm
• Photometric Range: 0.0 to 4.0 OD
• Photometric Accuracy: < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD
• Photometric Precision: < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD
• Light Source: Xenon flash lamp

• Temperature Range: Ambient + 4 °C up to 45 °C
• Temperature Uniformity: ± 0.5 °C @ 37 °C

Microplate Read Time:

• Endpoint
• 96-well: 9 seconds
• 384-well: 29 seconds
• Kinetic
• 96-well: 9 second minimum interval
• 384-well: 29 second minimum interval

Premier dual-mode readers for absorbance & fluorescence intensity-based assays. Users can select from top- or bottom-read modes for improved sensitivity for solution and cell-based assays.

Includes:

• SoftMax Pro software version 5.4.1
• Computer Workstation w/ Flat Screen Monitor
• Complete Set of Communication & Power Cables
• User/Reference Manuals for M2 and SoftMax Pro software

Specifications:

• Absorbance Photometric Performance
• Wavelength range: 200-1000 nm
• Wavelength selection: Monochromator
• Wavelength bandwidth: ≤ 4.0 nm
• Wavelength accuracy: ±2.0 nm
• Wavelength repeatability: ±0.2 nm

Fluorescence Photometric Performance (M2)

• Dual monochromators: 1 nm increments
• EX 250-850 nm
• EM 360-850 nm
• Bandwidth (EX, EM): 9, 9 nm
• Detection limit: 3.0 fmol/well FITC 200 μL in 96 wells