Used Lab Equipment

The Millipore Guava EasyCyte HT offers sample handling formats for both tubes and 96-well plates, direct sampling using patented microcapillary system, and a user-friendly, versatile software. EasyCyte instruments are also compatible with many fluorochromes and fluorescent reagents.

Specifications:

• Operating temperature: 16°–35°C (60°–95°F)
• Operating humidity: 10–90% relative humidity (non-condensing)
• Power: 100–240 VAC, 50/60 Hz, 120W
• Dimensions: 21 x 24 x 12 inches
• Weight: 71lb
• Detection: 5 colors including forward scatter
• Laser: blue 488 nm (50mW)
• Flow cell dimension: standard square capillary with ID of 100 µm
• Pump: 7 µL/min to 36 µL/min
• Sample flow rate: positive displacement
• Waste and cleaning vials: 30-mL glass vial with screw top
• Waste generation: typically <40 mL in 8 hours of continuous use
• Dead volume: 50 µL (for 96-well microplate); 75 µL (for 0.5-mL microcentrifuge tubes)
• Sample concentration: final particle concentration of 104 to 5 x 105 particles/ml
• Sample requirement: for accurate results (up to 2.5 x 106 beads/mL for the RapidQuant Assay) as few as 2,000 cells/test; typically 25,000-100,000 cells/test, depending on the assay
• Data management: Output data file formats - (1) binary data storage in FCS 3.0 format; (2) spreadsheet results file in CSV format

Single Laser Configuration

Includes Computer Loaded with Cytosoft Software

The guava easyCyte flow cytometry systems are cost-effective, easy to use and deliver complete and comprehensive cell analysis- right on your benchtop. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers—all while providing the analytical power you need to expand your research horizons.

Features:

• Blue Laser
• Forward and Side Scatter
• Tube Sampling
• Automated 96 Well Micrplate Sampling
• Small Sample Size (<20 μL)
• Minimal waste (<50 mL per day)
• Detectors
  • Green
  • Yellow
  • Red

Fluidics Specifications:

• Flow Cell Dimension: Standard square capillary with ID of 100 µm
• Pump: Positive displacement
• Sheath Fluid: None
• Sample Flow Rate: 7µL/min to 72 µL/min
• Waste Generation: typically <40mL in 8 hours of continuous use
• Dead Volume: 50 µL (for 96-well microplate)
• Sample Requirement: as few as 2,000 cells/test; typically 25,000 – 100,000 cells
• Cell Size: 1-60 µm
• Typical # of Cells/Protocol: 1,000 – 10,000 cells/test

Descriptions:

The Cogent M1 system is an easy-to-use bench-top TFF system that is ideally suited for process development, scaling studies, as well as clinical trials and small-scale production. An innovative and intelligent design, the system enables an extremely low hold-up volume for maximum volume concentration and optimal product recovery. Equipped with a 10-liter tank with embedded balance, the Cogent M1 system can also run in fed-batch or diafiltration mode using an optional transfer pump, which enables the system to process up to 100 liters or more based on product and membrane filter characteristics.

Specifications:

• Filtration Area: 0.1 sq. m to 1.14 sq. m
• Minimum Working Volume: 300 mL at 20% feed pump speed
• Starting Volume: Tank volume of 10 liters
• Hold-up Volume: < 10 mL (excluding cassette)
• Process Temperature Range: 4°C to 50°C (39°F to 122°F)
• Maximum Feed Flow Rate: 4.5 L/min at 5 bar g (72.5 psi)
• Minimum Feed Flow Rate: 200 mL/min
• Maximum Operating Pressure: 5 bar g (72.5 psi)
• Maximum System Pressure: 6 bar g (86 psi) before pump shut-off
• Optional Transfer Pump Flow Rate: Tubing dependant (0.16 to 2300 mL/mn)

The Direct Detect system provides accurate results without the pitfalls of colorimetric assays. By measuring amide bonds in protein chains, the system accurately determines an intrinsic component of every protein without relying on amino acid composition, dye binding properties or reduction-oxidation (redox) potential. Also, evaluation of other components of complex mixtures separable from the protein spectral region, like lipids, make lysates and membrane preps easier than ever.

Specifications:

• Assay Range: 250-5,000 μg / mL
• Sample Volume: 2 μL
• Protein Required to Measure 1 mg/mL: 6 μg
• Compatible: Detergents, Reducing agents, SDS, Chelators
• Time (To Analyze Single Samples): 2 mins

Specifications:

Flow rate:

• 1.3 – 1.7 LPM with Millipak® Express 20
• 1.3 – 1.7 LPM with BioPak

Resistivity:

• Minimum 18.0 MΩ.cm @ 25°C
• Typically 18.2 MΩ.cm @ 25°C

Particles: ≤ 1/ml (particle size > 0.22 μm)

Pyrogens: BioPak installed < 0.001 Eu/ml

TOC: Maximum 5 ppb (this specification came from test conditions where a Milli-Q Gradient was fitted with a Q-Gard® 1 Purification Pack and a Quantum EX Ultrapure Cartridge and the feedwater came from a Millipore RiOs Water System. The feedwater TOC value was < 50 ppb). The TOC of the Milli-Q Product Water can vary as a function of the feedwater TOC.

Micro-Organisms: ≤ 1 CFU/ml

Weight:

Milli-Q Gradient: Operating weight (wetted Q-Gard, Quantum and Millipak): 16 kg.

• Milli-Q Gradient A10: Operating weight: 16.8 kg.

Electrical: 

The electrical requirements for a Milli-Q Gradient (or Milli-Q Gradient A10) are:

• 120 VAC ± 10%, 50/60 Hz, 100 VA, 0.5 amp source, 2 amp Time Lag fuse used (ref. ZMQS6V0T1, MQP6V0T1, ZMQS6V001, ZMQP6V001).
• 230 VAC ± 10%, 50/60 Hz. 0.26 amp source, 1 amp Time Lag fuse used (ref. ZMQS5V0T1, ZMQP5V0T1, ZMQS5V001, ZMQP5V001).
• The source of electrical power should be within 1 meter of the Milli-Q. The source of electrical power must be earth grounded.

Measurement Ranges for TOC, Resistivity, Conductivity and Temperature:

• Resistivity Measurement Range: 2.4 – 18.2 MΩ.cm (compensated to a 25°C value)
• Conductivity Measurement Range: 0.055 – 0.294 μS/cm
• Temperature Measurement Range: 0 – 45°C (not the system temperature operating range)
• TOC Measurement Range: 1 – 999 ppb

Specifications:

Flow rate:

• 1.3 – 1.7 LPM with Millipak® Express 20
• 1.3 – 1.7 LPM with BioPak

Resistivity:

• Minimum 18.0 MΩ.cm @ 25°C
• Typically 18.2 MΩ.cm @ 25°C

Particles: ≤ 1/ml (particle size > 0.22 μm)

Pyrogens: BioPak installed < 0.001 Eu/ml

TOC: Maximum 5 ppb (this specification came from test conditions where a Milli-Q Gradient was fitted with a Q-Gard® 1 Purification Pack and a Quantum EX Ultrapure Cartridge and the feedwater came from a Millipore RiOs Water System. The feedwater TOC value was < 50 ppb). The TOC of the Milli-Q Product Water can vary as a function of the feedwater TOC.

Micro-Organisms: ≤ 1 CFU/ml

Weight:

Milli-Q Gradient: Operating weight (wetted Q-Gard, Quantum and Millipak): 16 kg.

• Milli-Q Gradient A10: Operating weight: 16.8 kg.

Electrical: 

The electrical requirements for a Milli-Q Gradient (or Milli-Q Gradient A10) are:

• 120 VAC ± 10%, 50/60 Hz, 100 VA, 0.5 amp source, 2 amp Time Lag fuse used (ref. ZMQS6V0T1, MQP6V0T1, ZMQS6V001, ZMQP6V001).
• 230 VAC ± 10%, 50/60 Hz. 0.26 amp source, 1 amp Time Lag fuse used (ref. ZMQS5V0T1, ZMQP5V0T1, ZMQS5V001, ZMQP5V001).
• The source of electrical power should be within 1 meter of the Milli-Q. The source of electrical power must be earth grounded.

Measurement Ranges for TOC, Resistivity, Conductivity and Temperature:

• Resistivity Measurement Range: 2.4 – 18.2 MΩ.cm (compensated to a 25°C value)
• Conductivity Measurement Range: 0.055 – 0.294 μS/cm
• Temperature Measurement Range: 0 – 45°C (not the system temperature operating range)
• TOC Measurement Range: 1 – 999 ppb

Data Module