Used Lab Equipment

The Millipore Guava EasyCyte HT offers sample handling formats for both tubes and 96-well plates, direct sampling using patented microcapillary system, and a user-friendly, versatile software. EasyCyte instruments are also compatible with many fluorochromes and fluorescent reagents.

Specifications:

• Operating temperature: 16°–35°C (60°–95°F)
• Operating humidity: 10–90% relative humidity (non-condensing)
• Power: 100–240 VAC, 50/60 Hz, 120W
• Dimensions: 21 x 24 x 12 inches
• Weight: 71lb
• Detection: 5 colors including forward scatter
• Laser: blue 488 nm (50mW)
• Flow cell dimension: standard square capillary with ID of 100 µm
• Pump: 7 µL/min to 36 µL/min
• Sample flow rate: positive displacement
• Waste and cleaning vials: 30-mL glass vial with screw top
• Waste generation: typically <40 mL in 8 hours of continuous use
• Dead volume: 50 µL (for 96-well microplate); 75 µL (for 0.5-mL microcentrifuge tubes)
• Sample concentration: final particle concentration of 104 to 5 x 105 particles/ml
• Sample requirement: for accurate results (up to 2.5 x 106 beads/mL for the RapidQuant Assay) as few as 2,000 cells/test; typically 25,000-100,000 cells/test, depending on the assay
• Data management: Output data file formats - (1) binary data storage in FCS 3.0 format; (2) spreadsheet results file in CSV format

Single Laser Configuration

Includes Computer Loaded with Cytosoft Software

The guava easyCyte flow cytometry systems are cost-effective, easy to use and deliver complete and comprehensive cell analysis- right on your benchtop. The culmination of over a decade of flow cytometry expertise, these instruments consume less sample, generate less waste and are easier to use and maintain than traditional flow cytometers—all while providing the analytical power you need to expand your research horizons.

Features:

• Blue Laser
• Forward and Side Scatter
• Tube Sampling
• Automated 96 Well Micrplate Sampling
• Small Sample Size (<20 μL)
• Minimal waste (<50 mL per day)
• Detectors
  • Green
  • Yellow
  • Red

Fluidics Specifications:

• Flow Cell Dimension: Standard square capillary with ID of 100 µm
• Pump: Positive displacement
• Sheath Fluid: None
• Sample Flow Rate: 7µL/min to 72 µL/min
• Waste Generation: typically <40mL in 8 hours of continuous use
• Dead Volume: 50 µL (for 96-well microplate)
• Sample Requirement: as few as 2,000 cells/test; typically 25,000 – 100,000 cells
• Cell Size: 1-60 µm
• Typical # of Cells/Protocol: 1,000 – 10,000 cells/test

The Direct Detect system provides accurate results without the pitfalls of colorimetric assays. By measuring amide bonds in protein chains, the system accurately determines an intrinsic component of every protein without relying on amino acid composition, dye binding properties or reduction-oxidation (redox) potential. Also, evaluation of other components of complex mixtures separable from the protein spectral region, like lipids, make lysates and membrane preps easier than ever.

Specifications:

• Assay Range: 250-5,000 μg / mL
• Sample Volume: 2 μL
• Protein Required to Measure 1 mg/mL: 6 μg
• Compatible: Detergents, Reducing agents, SDS, Chelators
• Time (To Analyze Single Samples): 2 mins

Features:

The Milli-Q Integral system includes a comprehensive and optimized sequence of water purification and monitoring technologies in a single unit, allowing both pure and ultrapure water to be produced from tap. It provides pressurized, bacteria-free water through E-POD units and Milli-Q ultra pure water through Q-POD units. Water can be delivered from the system's exclusive production unit to as many as 3 independent PODs that can be conveniently located at different placed in the lab. Each POD incorporates a color backlit screen enabling used to check the operation of the entire system and water quality at a glance.

Specifications:

• Feed Water Nature: Potable Tap Water
• Flow Rate: 10 Liters per hour
• Product Water Volume: 200L/day
• Product Water Conductivity: 0.056 uS/cm

Product Water Resistivity:

• Ultrapure Water: 18.2 MegOhm-cm
• Pure Water: >5 MegOhm-cm
• Product Water TOC: < 5 ppb
• Voltage: 100-240 V/50-60 Hz

Specifications:

Flow rate:

• 1.3 – 1.7 LPM with Millipak® Express 20
• 1.3 – 1.7 LPM with BioPak

Resistivity:

• Minimum 18.0 MΩ.cm @ 25°C
• Typically 18.2 MΩ.cm @ 25°C

Particles: ≤ 1/ml (particle size > 0.22 μm)

Pyrogens: BioPak installed < 0.001 Eu/ml

TOC: Maximum 5 ppb (this specification came from test conditions where a Milli-Q Gradient was fitted with a Q-Gard® 1 Purification Pack and a Quantum EX Ultrapure Cartridge and the feedwater came from a Millipore RiOs Water System. The feedwater TOC value was < 50 ppb). The TOC of the Milli-Q Product Water can vary as a function of the feedwater TOC.

Micro-Organisms: ≤ 1 CFU/ml

Weight:

Milli-Q Gradient: Operating weight (wetted Q-Gard, Quantum and Millipak): 16 kg.

• Milli-Q Gradient A10: Operating weight: 16.8 kg.

Electrical: 

The electrical requirements for a Milli-Q Gradient (or Milli-Q Gradient A10) are:

• 120 VAC ± 10%, 50/60 Hz, 100 VA, 0.5 amp source, 2 amp Time Lag fuse used (ref. ZMQS6V0T1, MQP6V0T1, ZMQS6V001, ZMQP6V001).
• 230 VAC ± 10%, 50/60 Hz. 0.26 amp source, 1 amp Time Lag fuse used (ref. ZMQS5V0T1, ZMQP5V0T1, ZMQS5V001, ZMQP5V001).
• The source of electrical power should be within 1 meter of the Milli-Q. The source of electrical power must be earth grounded.

Measurement Ranges for TOC, Resistivity, Conductivity and Temperature:

• Resistivity Measurement Range: 2.4 – 18.2 MΩ.cm (compensated to a 25°C value)
• Conductivity Measurement Range: 0.055 – 0.294 μS/cm
• Temperature Measurement Range: 0 – 45°C (not the system temperature operating range)
• TOC Measurement Range: 1 – 999 ppb

Specifications:

Flow rate:

• 1.3 – 1.7 LPM with Millipak® Express 20
• 1.3 – 1.7 LPM with BioPak

Resistivity:

• Minimum 18.0 MΩ.cm @ 25°C
• Typically 18.2 MΩ.cm @ 25°C

Particles: ≤ 1/ml (particle size > 0.22 μm)

Pyrogens: BioPak installed < 0.001 Eu/ml

TOC: Maximum 5 ppb (this specification came from test conditions where a Milli-Q Gradient was fitted with a Q-Gard® 1 Purification Pack and a Quantum EX Ultrapure Cartridge and the feedwater came from a Millipore RiOs Water System. The feedwater TOC value was < 50 ppb). The TOC of the Milli-Q Product Water can vary as a function of the feedwater TOC.

Micro-Organisms: ≤ 1 CFU/ml

Weight:

Milli-Q Gradient: Operating weight (wetted Q-Gard, Quantum and Millipak): 16 kg.

• Milli-Q Gradient A10: Operating weight: 16.8 kg.

Electrical: 

The electrical requirements for a Milli-Q Gradient (or Milli-Q Gradient A10) are:

• 120 VAC ± 10%, 50/60 Hz, 100 VA, 0.5 amp source, 2 amp Time Lag fuse used (ref. ZMQS6V0T1, MQP6V0T1, ZMQS6V001, ZMQP6V001).
• 230 VAC ± 10%, 50/60 Hz. 0.26 amp source, 1 amp Time Lag fuse used (ref. ZMQS5V0T1, ZMQP5V0T1, ZMQS5V001, ZMQP5V001).
• The source of electrical power should be within 1 meter of the Milli-Q. The source of electrical power must be earth grounded.

Measurement Ranges for TOC, Resistivity, Conductivity and Temperature:

• Resistivity Measurement Range: 2.4 – 18.2 MΩ.cm (compensated to a 25°C value)
• Conductivity Measurement Range: 0.055 – 0.294 μS/cm
• Temperature Measurement Range: 0 – 45°C (not the system temperature operating range)
• TOC Measurement Range: 1 – 999 ppb

Features:

The Muse Cell Analyzer delivers accurate assessments of cell health parameters, cell signaling activation pathways & immune status in just minutes, revolutionizing the way you analyze cells.

Specifications:

• Cell Number per Assay: User defined; cell concentrations of 10,000–500,000 cells/mL
• Sample Format: Single loader
• Sample Volume: 200 µL
• Emission Max: Yellow: 28nm bandwidth at 576nm, Red: 30nm bandwidth at 680nm, Forward scatter = laser wavelength
• Excitation Max: Green Laser (532nm)
• Scatter: Forward
• Throughput: Less than 2 minutes per sample
• Dimensions 8.69” H  x 8.12” W x 11.11” L

Data Module