Used Lab Equipment

Comes with computer loaded w/ Unicorn Software

The purification of biological therapeutics requires processes which usually employ two technologies used in combination: membrane separations and chromatography. Chromatography offers greater selectivity than membrane separations, and can purify much greater quantities of solution in one operation.

However, membrane separations technologies offer a number of other advantages including the fact that they are a fast and robust process step.

ÄKTAcrossflow covers three main filtration techniques:

• Ultrafiltration of proteins
• Diafiltration of proteins
• Microfiltration of cell and lysate solutions

The system supports both hollow fibers and flat sheet cassettes with sizes suitable for flow rates up to 300 ml/min for flat sheet cassettes and up to 600 ml/ min for hollow fibers.

The main application is method development and scale-up in process development laboratories.

Specifications:

• Main Voltage: 100 to 240 V~ ±10%, 50–60Hz (Auto range)
• Weight: 70 kg
• Recirculation Volume Min.: 25 ml (with 1.7 mm i.d. tubing kit)
• Width: 620 mm
• Application: Well-suited for process development, filter screening, and process optimization at small scale.
• Compliance: The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from GE Healthcare and connected only to other CE labeled GE Healthcare modules or other products as recommended. For regulatory details please see declaration of conformity.
• Flow Cell Path Length: 2 mm
• Connections: UNF 5/16"
• Wavelength [Zn Lamp]: 214 nm
• Hold-Up Volume Recirculation Line: < 20 ml (with 1.7 mm i.d. tubing kit, empty reservoir)
• Length: 400 mm
• Operating Pressure Max.:2 bar (75.4 psi)
• Material Compliance: USP Class V
• Height: 650 mm
• Protection Class: IP 20
• Complete Packsize: 1 Piece
• pH Range: 2 - 12
• Mixing Principle: Magnetic stirrer

AKTA FPLC system is a fully automated liquid chromatography system designed for research scale purification of proteins, wherein proteins of various sizes can be readily purified using different kinds of columns.

Features:

• P-920 Pump
• UPC-900
• Frac-900 Fraction Collector
• INV-907 Injection Valve
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• Box 900: Storage compartment and Solvent Tray
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 
• Manuals

Features:

AKTAmicro, including BOX900, PH/C-900. UV-900,P-905

Micro Purification and purity analysis of biomolecules at submicrogram levels

Specifications:

• Voltage: 100-240V
• Frequency: 50-60Hz
• Max Power: 600 VA
• Fuse: T 6,3AL 250V
• Lamp intensity:210nm 69% 300nm 70%

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

Features:

• Fraction collector
• pH Electrode
• UV Monitor
• Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

Features:

• Fraction collector
• pH Electrode
• UV Monitor
• Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

Features:

• Fraction collector
• pH Electrode
• UV Monitor
• Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Features:

• Simple column selection with huge built-in library
• Automatic titration of buffers across a broad pH range
• Quick and easy transfer of methods to production-scale
• UNICORN method wizard High performance purification and characterization
• State-of-the-art computer-controlled pumps, UV/conductance monitors, and fraction collector
• UV-Vis detection of up to three wavelengths simultaneous

Specifications:

• Flow rate: 0.01-100 ml/min
• Operating pressure max.: 10 MPa
• Tubing i.d. (flow path): 0.75 mm
• UV wavelength: 190-700 nm
• Flow cell path length: 2 mm
• pH monitoring: 2–12
• Buffer selection: 11 buffer inlets
• Relative humidity: 10%-95% (non condensing)
• pH Stability: 1-14
• Viscosity: max 5 cP
• Multiple wavelength detection: Yes, up to 3 wavelengths
• Multiple sample injection: Yes, up to 8 samples
• Autosampler injection: Optional
• Automatic buffer preparation: BufferPrep
• Pump type: Piston pump, metering type
• Application: Purification of microgram to gram quantities of protein
• Dimensions (W x H x D): 480 x 610 x 470 mm
• Power Requirements: 100-120/220-240 VAC, 600W

ÄKTAxpress Module

ÄKTA xpress is a chromatography system designed for automated, multistep protein purification of both single and multiple samples.

• Unattended purification: Built-in purification knowledge combines software, hardware, and prepacked columns to eliminate time-consuming manual tasks.
• Highest possible purity: Optimized protocols with up to four steps will deliver tagged proteins of near 100% purity. Built-in cleaning procedures between MAb samples to minimize carry over
• High productivity: Each module can purify up to four proteins. The modular design offers adaptability to meet increased throughput needs.
• Yield : The system is capable of purifying target proteins in yields varying from 1 to 50+ mg.
• Akta Express has a Small footprint: A compact design allows the system to occupy minimal space on the bench or in the cold room.

ÄKTAxpress Module

• Complete Packsize: 1 Piece
• Application: Unattended multistep purification of tagged proteins and antibodies
• Flow Rate: 0.1-65 ml/min
• Operating Pressure Max.: 3 MPa (435 psi)
• Tubing i.d. [Flow Path]: 1 mm
• Single Wavelength Detection: Mercury lamp and 254 and 280 nm filters included. Optional filters for 313, 365, 405, 436 and 546 nm.
• Multiple Wavelength Detection: No
• Flow Cell Path Length: 2 mm
• Multiple Sample Injection: Yes, up to 4 samples
• Autosampler Injection: No
• Automatic Buffer Preparation: No
• Buffer Selection: 10 buffer inlets
• Multi-Step Purification: Yes, up to 4 steps
• Relative Humidity: 20%-95% (non condensing)
• pH Stability Long Term: 2-12
• pH Stability Short Term: 1-14
• Viscosity: max 5 cP
• Width: 250 mm
• Height: 660 nm
• Depth: 600 mm
• Weight: 30 kg
• Voltage: 100-120/220-240 VAC
• Power Consumption: 120 VA
• Protection Class: IP 20
• Pump Type: Piston pump, metering type

This can be purchase with a number of optional detectors.
Description - The Waters 2695 features a large, easy-to-use LCD interface. Scientists can take advantage of the quick autostart and optimize their methods with a simple push of a button. Manage up to 120 industry standard vials with the built in autosampler. This reliable tool is a common work-horse in most analytical and contract labs. Built to meet labs rigorous requirements or routine analysis, this robust instrument is easily found in Pharmaceutical, Food Safety, R&D, Chemistry, Environmental or Quality Control labs. Simplify how you collect data, built-in compliance and validation features. The Empower software is capable of automating method development, archiving methods and customizing data reports.

The components can be customized to your needs.

You can get the system with any of the detectors, as well as an optional Computer.

• 996 PDA Detector
• 2996  PDA Detector
• 2998 PDA Detector
• 2487 UV detector
• 2489 UV Detector
• 2475 FLD detector:
• Waters 2410 Refractive Index Detector:
  
• Computer Loaded with Empowers 2 Software

This can be purchase with a number of optional detectors.
Description - The Waters 2695 features a large, easy-to-use LCD interface. Scientists can take advantage of the quick autostart and optimize their methods with a simple push of a button. Manage up to 120 industry standard vials with the built in autosampler. This reliable tool is a common work-horse in most analytical and contract labs. Built to meet labs rigorous requirements or routine analysis, this robust instrument is easily found in Pharmaceutical, Food Safety, R&D, Chemistry, Environmental or Quality Control labs. Simplify how you collect data, built-in compliance and validation features. The Empower software is capable of automating method development, archiving methods and customizing data reports.

The components can be customized to your needs.

You can get the system with any of the detectors, as well as an optional Computer.

• 996 PDA Detector
• 2996  PDA Detector
• 2998 PDA Detector
• 2487 UV detector
• 2489 UV Detector
• 2475 FLD detector:
• Waters 2410 Refractive Index Detector:
  
• Computer Loaded with Empowers 2 Software

High-performance, fully automated imaging has made the AlphaImager HP our most popular platform. The system's application versatility not only includes a wide range of fluorescent and colorimetric applications, but can also be expanded to chemiluminescence as laboratory needs change.

Specifications:

• Camera Resolution: 4 megapixels
• A/D: 12-bit
• Lens: 8 – 48 mm, f/1.2 Zoom, motorized
• Maximum Field of View: 42 x 31 cm
• Filter Positions: 5, motorized
• Size: 15 x 20 x 24 in
• Weight: 80 lbs

Bruker AMX-300 Nuclear Magnetic Resonance Spectrometer with the "piano" style console and an unshielded Oxford 300MHz magnet. The magnet was de-energized about five years ago. All of the kits for moving the magnet are included.  

This is a 2-channel system with a solids accessory rack with high power 1H and X transmitters. Three probes included (plus a Doty solid state probe): a 5mm 1H inverse probe, a 10mm BB, and a standard bore magic angle solids probe (7 & 4mm stators).