Cambridge Scientific
Used Lab Equipment

Used Lab Equipment

We offer an assortment of used and refurbished Production Equipment ranging from fermenters, Bioreactors, Homogenizers, Chromotography Bioprocess Units to Production Scale Centrifuges. Some of our popular manufacturers include New Brunswick, Chemap, and Carr.

Oxygen Controller: 1-15 of 21

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Shimadzu DGU-14A HPLC Degasser

Shimadzu DGU-14A HPLC Degasser

Cambridge Scientific ID #'s: 18317, 4849, 12795, 12797, 9660, 12778, 9419, 9507, 12796, 12281, 12164, 11956

Features:

  • Ultra-compact and stackable in design.
  • Providing a fast and efficient way to obtain superior degassing of up to four solvents.
  • For stubborn degassing problems, channels may be connected in series to supply even greater degassing efficiency.
  • The four-channel in-line degasser maintains a strong, steady vacuum to provide the highest level of degassing efficiency.
  • Maintaining a dissolved oxygen concentration of 2.0 ppm or less at flow rates of up to 3.0 mL/min (1.5 ppm or less at 1.0 mL/min)
  • Can operate at flow rates of up to 20 mL/min per flow line.
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Plas-Labs 855-AC *NEW* Glove Box

Plas-Labs 855-AC *NEW* Glove Box

Cambridge ID #: 17527

Features:

Ideal for situations requiring low levels of oxygen during critical isolation of your research materials.

Specifications:

 

  • Internal Dimensions: 41"w x 28"d x 26"h, 104cm x 71cm x 66cm
  • External Dimensions: 55"w x 35"d x 38"h, 140cm x 89cm x 97cm
  • Volume: 17.3 cu. Ft., 489 L
  • Power requirements: 120 Volts 60 Hz.
  • Formed one piece clear acrylic top.
  • Self-sealing quick disconnects enable the operator to change the drying train without disturbing the internal atmosphere. Matched die molded thermoset white bottom.
  • Adjustable vacuum gauge on transfer chamber.
  • Two vacuum pressure pumps, one each for transfer chamber and drying train.
  • Four ground key cock valves for purging.
  • Drying train includes polycarbonate canisters containing DesiSphereTM.
  • Inner door on transfer chamber opens automatically when pressures are neutralized between main chamber and transfer chamber.
  • All controls are illuminated.
  • Transfer chamber is 12" long and 11" diameter (Interior Dimension).
  • "Bright Light" system (40,000 hour lamp).
  • Electrical outlet strip; U.L. & C.S.A. approved, hospital grade with four outlets.
  • White ambidextrous Hypalon gloves.
  • All clamps are adjustable to compensate for wear.

 

 

 

 

 

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Plas-Labs 856-HYPO *NEW* Glove Box

Plas-Labs 856-HYPO *NEW* Glove Box

Cambridge ID #: 17528

Features:

Automatically controls oxygen, C02, and temperature. Ideal for tumor cell and stem cell research. The Hypoxia Chamber Glove Box is ideal for tissue culture work, including tumor cell and stem cell research. It features a color operated "touch screen" for automatically controlling oxygen levels, temperature levels, and carbon dioxide levels. This new controlled Hypoxia Chamber is available with up to six (6) individually controlled compartments inside the  glove box. A side chamber is available for sample preparations and / or a microscope viewing port. Each cubicle has its own temperature, oxygen, and CO2 controls. It also includes a side access door and transfer chamber.

Specifications:

  • Interior Dimensions: 41"w x 28"d x 26"h
  • External Dimensions: 60"w x 38"d x 31"h *width includes transfer chamber
  • 8" Color Panel Touch Screen.
  • Thermoelectric Assembly (Peltier device) O2 Sensor and CO2 sensor.
  • Password protected, configurable PID control.
  • Platinum RTD with sealing gland.
  • One touch, on-screen datalogging.
  • N2, CO2, and O2 gas hook-up.
  • Air circulation fan Inert gas hook-up.
  • Configurable for up to six individually controlled sampling chambers.
  • Ethernet port connection for data logging.

 

 

 

 

 

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Plas-Labs 857-OTA *NEW* Glove Box

Plas-Labs 857-OTA *NEW* Glove Box

Cambridge ID #: 17529

Features:

The 857-OTA One-Touch "Go Anaerobic" chamber allows one press of the "Go Anaerobic" button to create a strict anaerobic environment. Simply push the "Go Anaerobic" button on the large, color, touch-screen display and glove box automatically begins the purging sequence.  Only 300 liters of gas needed to achieve anaerobic atmosphere. The 857-OTA Anaerobic Chamber includes automatic purging of main chamber and transfer chamber, oxygen analyzer, pressure hold function, and a large easy-to-use touch screen.

Specifications:

 

  • Internal Dimensions: 41"w x 28"d x 26"h, 104cm x 71cm x 66cm
  • External Dimensions: 55"w x 35"d x 38"h, 140cm x 89cm x 97cm
  • Volume: 17.3 cu. Ft., 489 L
  • O2 Sensor Accuracy (%): 0-100.0%   ±1.0%
  • O2 Sensor Accuracy (ppm): 0-10,000   ±1.0% FS
  • Temperature Range: 5-90% Rh             
  • Temperature accuracy:  ±1.0%
  • Large, color, "Touch Screen" display
  • "Go Anaerobic" automatic purging
  • Three Drying Train canisters with quick disconnects.
  • White ambidextrous Hypalon gloves.
  • Programmable Catalyst Heater Unit.
  • Two ground key-cock valves on main chamber.
  • "Bright Light" System. Two 1/4" NPT gas connections.
  • Transfer chamber (12" long x 11" Interior Dimension)

 

 

 

 

 

 

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Thermo Scientific 3310 CO2 Incubator

Thermo Scientific 3310 CO2 Incubator

Cambridge Scientific ID #'s: 17374, 17373

Features:

This CO2 incubator is equipped with a high level of contamination control for high volume culturing. It is built with multiple advanced components including: CO2 sensor, precise microprocessor controls, active humidity control, HEPA air filtration and high-temperature decontamination capability.

Specifications:

  • Capacity: 11.4 cu.ft., 322.8L
  • Temperature Range (Metric): Ambient +5° to 50°C
  • Chamber Material: Polished stainless steel
  • CO2 Concentration Range: 0 to 20%
  • CO2 Sensor Technology: IR sensor
  • Oxygen Control: Not Available
  • Humidity Delivery: External humidity reservoir
  • Relative Humidity: Ambient to 95%, controllable
  • Electrical Requirements: 115V 50/60 Hz
  • Dimensions (D x W x H) Interior: 20.6 x 28.8 x 32.8 in. (52.3 x 73.2 x 83.8cm)
  • Dimensions (L x W x H) Exterior: 27 x 43 x 39.4 in. (68.6 x 109.2 x 100.2cm)
  • Net Weight: 410 lb, 186kg
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Coy Laboratories 2000 Oxygen Controller

Coy Laboratories 2000 Oxygen Controller

Cambridge ID #: 17059

The Oxygen Controller utilizes a unique galvanic sensor to measure up to 100% concentration in atmospheric environment. The sensor is temperature compensated and completely sealed from the environment.

Specifications:

 

  • Control Range: 0 to 60%, O2 Atmospheric
  • Measurement: 0 to 100%
  • Precision: 0.1%
  • Set Point Range: Selectable (0 to 100%)
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Shel Lab SI6 Shaking Incubator

Shel Lab SI6 Shaking Incubator

Cambridge Scientific ID #'s: 16667, 16668

Provide a controlled environment for continuous growth of biological organisms. Market applications include: microbiology, tissue culture, cell culture and bacteriology. Features a unique counterbalance weighting system that is adjustable to accommodate off-center loads, varying capacities and stroke length, for smoother operation. An adjustable orbit can change rotational stroke to accommodate different types of cells and provide maximum oxygen transfer.

Specifications:

 

  • Temperature Range: 8 to 60°C
  • Capacity: 156 L
  • Speed: 30-400 RPM
  • Dimensions: 29 D x 28.5 W x 42" H
  • Weight: 90 kg

 



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Bacharach Fyrite Gas Analyzer

Bacharach Fyrite Gas Analyzer

Cambridge ID #: 16493

The Fyrite Classic is a tried-and-true instrument for measuring oxygen and carbon dioxide. The Fyrite Classic is available for O2 or CO2, and each gas type of Fyrite is available in multiple ranges to meet a variety of applications, from combustion analysis to incubators to anesthesiology.

The Fyrite Classic contains fluid that is specifically designed to absorb O2 or CO2 with minimal cross-sensitivities to other gases, which leads to accurate readings. It is easy to test the fluids strength and replace it when needed which means no need to return the unit for service and no downtime.

Specifications:

  • Measurements:
    • O2: 0 to 7.6, 0 to 21 and 0 to 60%
    • CO2: 0 to 7.6, 0 to 20 and 0 to 60%
  • Maximum Number of Samples:
    • O2: 100
    • CO2: 300
  • Resolution:
    • 0 to 7.6% range: 0.2%
    • 0 to 20/21% range: 0.5%
    • 0 to 60% range: 2%
  • Size (H x W x D): 4 x 3.1 x 3.1 in. (18.8 x 7.9 x 7.9 cm)
  • Weight: 2 lb. (0.54 kg) unfilled
  • Operating Conditions: -30 to 150 °F (-34 to 65 °C)
  • Maximum Gas Temperature: 850 °F (454 °C)
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Perimed Periflux System 5001 Laser Doppler Perfusion Monitor

Perimed Periflux System 5001 Laser Doppler Perfusion Monitor

Cambridge ID #: 16199

The modular design enables the simultaneous monitoring of blood flow/perfusion and transcutaneous oxygen and/or carbon dioxide. Function units for heat provocation and pressure control are also available as well as a wide range of laser Doppler probes.Combined with Perimed’s dedicated software, the system offers complete and customized solutions for safe, smooth and simple workflows to investigate and assess the microcirculation.

Specifications:

  • Type (protection against electric shock): Class I Equipment, Type BF and/or CF depending on function units
  • Laser (only PF 5001): IEC 60825-1:2007 Class 1 (CFR 1040.10 Class I) Solid-state diode laser, 780 nm wavelength.
  • Dimensions: 30 x 32 x 10.5 cm
  • Weight: 7.7 kg (with four function units)
  • Operating conditions: +10 to +35 °C at 30-85% RH
  • Computer (Minimum requirements): CPU 800 MHz, 256 MB RAM Windows 2000/XP
  • Output:
    • Digital: RS-232 output to computer or directly to printer
    • Analog: -2.5 to +10 V
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Perimed PeriFlux System 5000 Laser Doppler Perfusion Monitor

Perimed PeriFlux System 5000 Laser Doppler Perfusion Monitor

Cambridge ID #: 15826

The modular design enables the simultaneous monitoring of blood flow/perfusion and transcutaneous oxygen and/or carbon dioxide. Function units for heat provocation and pressure control are also available as well as a wide range of laser Doppler probes.Combined with Perimed’s dedicated software, the system offers complete and customized solutions for safe, smooth and simple workflows to investigate and assess the microcirculation.

Specifications:

  • Type (protection against electric shock): Class I Equipment, Type BF and/or CF depending on function units
  • Laser (only PF 5001): IEC 60825-1:2007 Class 1 (CFR 1040.10 Class I) Solid-state diode laser, 780 nm wavelength.
  • Dimensions: 30 x 32 x 10.5 cm
  • Weight: 7.7 kg (with four function units)
  • Operating conditions: +10 to +35 °C at 30-85% RH
  • Computer (Minimum requirements): CPU 800 MHz, 256 MB RAM Windows 2000/XP
  • Output:
    • Digital: RS-232 output to computer or directly to printer
    • Analog: -2.5 to +10 V
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Life Technologies iBlot 2 Dry Blot System

Life Technologies iBlot 2 Dry Blot System

Cambridge Scientific ID #'s: 15207, 17640, 16497, 15208, 17810

The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)

  • Complete protein transfer in 7 minutes or less
  • High detection sensitivity and even transfer
  • Increased blotting reliability and reproducibility
  • Flexible gel size formats and membrane types
  • A simple, user-friendly system
  • Options to create new custom programs
  • Built-in tutorial and application notes
  • High-quality transfer stacks that are more compact than before

Rapid, High-quality Protein Transfers

  • For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
  • Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
  • Allows users to create custom programs

iBlot 2 Transfers Save Time

  • With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
  • The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques

How It Works

  • Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
  • The high density of ions in the gel matrix enables rapid protein transfer
  • During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
  • Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
  • Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer

Proteins, Expression, Isolation and Analysis, Western Blotting

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Servomex 04200C1 Gas AnalyzerImage Coming Soon

Servomex 04200C1 Gas Analyzer

Cambridge ID #: 7119
  • Industrial Gas Analyzer. New in box. Used for composition of carbon dioxide, carbon monoxide, methane or oxygen in a flowing (dry) stream.
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    Carlisle Barrier System AMP2000 Glove BoxImage Coming Soon

    Carlisle Barrier System AMP2000 Glove Box

    Cambridge ID #: 11525

    Features:

    • 4 glove isolator
    • highly aseptic environment for transfer of sterility test materials
    • The isolator has been designed with an emphasis on ergonomics and PLC control
    • Size of the isolator: 60”W x 26”D
    • Enclosure height: 30”
    • The operator will access storage shelves via three glove ports
    • Isolator construction is of 316L stainless steel with laminated safety glass windows, in silicone channel gaskets
    • An inlet and outlet blower system will exhaust hydrogen peroxide vapors through 2” automatic ball valves, controlled by the PLC for fully automated decontamination cycle with a VHP-1000 generator by Steris Corporation. Under normal operations, the inlet blower will slightly pressurize the isolator through HEPA filters, providing a minimum Class 100 environment.
    • Product and test materials are entered into the isolator via the large hatch back window and stored on shelves
    • After the window is sealed, the VHP decontamination cycle can be initiated.
    • Upon completion of the decontamination cycle, the operator docks the 350mm RTP beta flange to the alpha flange found on the work center (testing) isolator. The door is opened from the work center isolator. After the door is opened, the operator enters the glove ports and passes the required materials into the work center isolator for testing.
    • After testing, the operator passes media samples into the transfer isolator.
    • The transfer isolator is taken to incubators, where sealed media containers are removed from the isolator and placed into Amgen’s incubators.
    • Other waste material can be removed from the isolator and any cleaning can take place. The isolator will need to be decontaminated again for the next sterility test.

     Chamber Differential Pressure (DP) Controls

    • Sampling Method: Chamber pressure is sampled using  .250"OD X .125" ID polyurethane tubing. One pressure port on the transducer is terminated to a dedicated sense port penetrating the isolator chamber. The second pressure port is left open to the room. The pressure transducer is located to facilitate short tubing runs to decrease pressure drops and signal latency.
    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's DP Set Point (DP SP).
    • Process Variable (PV): An appropriately ranged pressure transducer continuously senses the differential between the isolator chamber and the ambient or room air pressure. The transducer converts and transmits the air pressure signal to an electrical signal. The electrical signal format is typically a 4-20ma analog signal.  The 4-20ma signal is routed to an analog input at the PLC. The program will translate the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's Differential Pressure Process Variable (DP PV).
    • Control Variables (CV): The PLC program will scale the process variable to appropriate units of pressure (typically INCHES of H2O). Each individual operating phase will use the scaled PV values & SP values for enabling pressure control logic & adjustment of outputs to associated devices, numeric or graphical displays at the HMI, and conditional logic for alarms.

    Chamber Oxygen (O2) Controls:

    • Sampling Methhods: Two different methods of oxygen sampling are used by Carlisle Barrier Systems.The first and simplest method is continuous sampling.  With this method the oxygen sensor is directly located within the isolator chamber.  The transducer is then exposed to, and sampling, the isolator atmosphere at all times.The second method of oxygen sampling is to employ a sampling pump. With this method the pump pulls an air sample from the isolator chamber and passes the atmosphere sample past the oxygen sensor. The pump can then be enabled/disabled  for different phases of operation
    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's O2 Set Point (O2 SP).
    • Process Variable (PV):  The O2 transducer continuously senses the chamber O2 level. The transducer converts and transmits the oxygen signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's O2 Process Variable (O2 PV).
    • Control Variables (CV): The program will scale the process variable to appropriate units of oxygen (typically % O2). Each individual operating phase will use the scaled PV & SP values for enabling control logic & outputs to associated devices, numeric or graphical displays at the HMI, and conditional logic for alarms.

     Chamber Relative Humidity (RH) Controls:

    Sampling Method:The transducer is typically inserted directly into the isolator chamber. Unless otherwise capped off the transducer is continuously exposed to chamber atmosphere.

    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is a numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's RH Set Point (RH SP).
    • Process Variable (PV): The RH transducer continuously senses the chamber RH level. The transducer converts and transmits the oxygen signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's RH Process Variable (RH PV).
    • Chamber Temperature  (Deg F) Control: The Chamber temperature is continuously sensed, translated to a 4-20ma analog signal, and input into the PLC thru an analog input. The temperature signal is then scaled accordingly, posted to the HMI screen, and compared to the appropriate temperature set point. If the controls and alarms are enabled, appropriate modifications are made to the temperature control signal, and alarm indications.

    Chamber Temperature (Deg F) Control:

    Sampling Method:The temperature transducer is inserted directly into the isolator chamber. Unless otherwise capped off the temperature sensor is continuously exposed to the isolator atmosphere. When RH sensing is present on the isolator, the temperature sensor is typically combined with this sensor. If there is no RH sensing present then an independent temperature-sensing probe is used. Without RH sensing present, the sensing transducer is typically an industrial RTD. With RH sensing present the sensing transducer is typically an integrated chip (IC).

    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is a numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's RH Set Point (RH SP).
    • Process Variable (PV):  The RH transducer continuously senses the chamber RH level. The transducer converts and transmits the RH signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's RH Process Variable (RH PV).

    Chamber Vaporized Hydrogen Peroxide (VHP) Level

    Sampling Methods: Sampling pumps are used to pull a small portion of the isolator's atmosphere past the VHP sensor(s). The sampled atmosphere is pumped through .250"OD X .125" ID VITON or TEFLON tubing passed by the VHP sensor and returned to the chamber.  The sample pump(s) are configured to run based on different the different conditional requirements for each phase of operation.

    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's VHP Set Point (VHP SP).
    • Process Variable (PV):  The VHP transducer continuously senses the VHP level present in the sampling system. The transducer converts and transmits the VHP signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's VHP Process Variable (VHP PV).

     

    Chamber HEPA Pressure Level

    Sampling Method: Chamber inlet and exhaust HEPA filter pressure is sampled using  .250"OD X .125" ID polyurethane tubing. The pressure transducers are continuously exposed to chamber atmosphere.

    • One pressure port on the each respective pressure transducer will be terminated to a dedicated pressure sense port on the "non-chamber side" of the inlet or exhaust port HEPA filter. The second pressure sense port on each respective transducer will be tee'd together and terminated to a dedicated pressure port penetrating the isolator chamber. This tubing method generates a differential pressure measurement acrossed the HEPA relative to chamber pressure.
    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into a Phase Parameter Screen accessible from the HMI. The set point variable associated with the HEPA filter pressures used only for alarm functions.
    • Process Variable (PV):An appropriately ranged pressure transducer senses the resulting differential pressure. The transducer converts and transmits the air pressure signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is  the HEPA filter Differential Pressure Process Variable (HEPA DP PV).

    Control and Indications Variables

    As stated above control function associated with HEPA filter pressure variables is limited to alarm. 

    Chamber Vapor Level

    • Sampling Methods: The vapor sensor is directly located within the isolator chamber.  Unless otherwise capped off, the transducer is exposed to, and sampling, the chamber atmosphere at all times.
    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's Vapor Set Point (VPR SP).
    • Process Variable (PV):  The vapor transducer continuously senses the vapor level. The transducer converts and transmits the vapor signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the Vapor Process Variable (VPR PV).
    • Control  and Indication Variables (CV): The program will scale the process variable to appropriate units of vapor (typically % vapor). Each individual operating phase will use the scaled PV & SP values for enabling control logic & outputs to associated devices, numeric or graphical displays at the HMI, and conditional logic for alarms

    Specifications:

    • Chamber Exterior Size: Length x Width x Height: Approx. 60”L X 26” W X 30” H
    • Overall Equipment Size: Length x Width x Height: Approx. 65”L X 33” W X 86” H
    • Shell: 316 L S.S
    • Stand: 304 S.S
    • Finishing: 304 S.S
    • Windows: Glass windows
    • HVAC Piping: 316 L S.S
    • Filters: HEPA
    • Gas: CDA(type of gas); regulator;
    • Humidity chamber: monitor
    • Utility Panel Compression Fitting: 2, teflon, 3”/8” Compression
    • Power: 125V, 60 Hz
    • Compressed air : Yes
    • Pressure: 90 Psi
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    Carlisle-Barrier-System-AMP2000-Glove-Box-11525
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    Walker Barrier Systems AMP2000 Glove BoxImage Coming Soon

    Walker Barrier Systems AMP2000 Glove Box

    Cambridge ID #: 13259

    Features:

    • 4 glove isolator
    • highly aseptic environment for transfer of sterility test materials
    • The isolator has been designed with an emphasis on ergonomics and PLC control
    • Size of the isolator: 60”W x 26”D
    • Enclosure height: 30”
    • The operator will access storage shelves via three glove ports
    • Isolator construction is of 316L stainless steel with laminated safety glass windows, in silicone channel gaskets
    • An inlet and outlet blower system will exhaust hydrogen peroxide vapors through 2” automatic ball valves, controlled by the PLC for fully automated decontamination cycle with a VHP-1000 generator by Steris Corporation. Under normal operations, the inlet blower will slightly pressurize the isolator through HEPA filters, providing a minimum Class 100 environment.
    • Product and test materials are entered into the isolator via the large hatch back window and stored on shelves
    • After the window is sealed, the VHP decontamination cycle can be initiated.
    • Upon completion of the decontamination cycle, the operator docks the 350mm RTP beta flange to the alpha flange found on the work center (testing) isolator. The door is opened from the work center isolator. After the door is opened, the operator enters the glove ports and passes the required materials into the work center isolator for testing.
    • After testing, the operator passes media samples into the transfer isolator.
    • The transfer isolator is taken to incubators, where sealed media containers are removed from the isolator and placed into Amgen’s incubators.
    • Other waste material can be removed from the isolator and any cleaning can take place. The isolator will need to be decontaminated again for the next sterility test.

     Chamber Differential Pressure (DP) Controls

    • Sampling Method: Chamber pressure is sampled using  .250"OD X .125" ID polyurethane tubing. One pressure port on the transducer is terminated to a dedicated sense port penetrating the isolator chamber. The second pressure port is left open to the room. The pressure transducer is located to facilitate short tubing runs to decrease pressure drops and signal latency.
    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's DP Set Point (DP SP).
    • Process Variable (PV): An appropriately ranged pressure transducer continuously senses the differential between the isolator chamber and the ambient or room air pressure. The transducer converts and transmits the air pressure signal to an electrical signal. The electrical signal format is typically a 4-20ma analog signal.  The 4-20ma signal is routed to an analog input at the PLC. The program will translate the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's Differential Pressure Process Variable (DP PV).
    • Control Variables (CV): The PLC program will scale the process variable to appropriate units of pressure (typically INCHES of H2O). Each individual operating phase will use the scaled PV values & SP values for enabling pressure control logic & adjustment of outputs to associated devices, numeric or graphical displays at the HMI, and conditional logic for alarms.

    Chamber Oxygen (O2) Controls:

    • Sampling Methhods: Two different methods of oxygen sampling are used by Carlisle Barrier Systems.The first and simplest method is continuous sampling.  With this method the oxygen sensor is directly located within the isolator chamber.  The transducer is then exposed to, and sampling, the isolator atmosphere at all times.The second method of oxygen sampling is to employ a sampling pump. With this method the pump pulls an air sample from the isolator chamber and passes the atmosphere sample past the oxygen sensor. The pump can then be enabled/disabled  for different phases of operation
    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's O2 Set Point (O2 SP).
    • Process Variable (PV):  The O2 transducer continuously senses the chamber O2 level. The transducer converts and transmits the oxygen signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's O2 Process Variable (O2 PV).
    • Control Variables (CV): The program will scale the process variable to appropriate units of oxygen (typically % O2). Each individual operating phase will use the scaled PV & SP values for enabling control logic & outputs to associated devices, numeric or graphical displays at the HMI, and conditional logic for alarms.

     Chamber Relative Humidity (RH) Controls:

    Sampling Method:The transducer is typically inserted directly into the isolator chamber. Unless otherwise capped off the transducer is continuously exposed to chamber atmosphere.

    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is a numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's RH Set Point (RH SP).
    • Process Variable (PV): The RH transducer continuously senses the chamber RH level. The transducer converts and transmits the oxygen signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's RH Process Variable (RH PV).
    • Chamber Temperature  (Deg F) Control: The Chamber temperature is continuously sensed, translated to a 4-20ma analog signal, and input into the PLC thru an analog input. The temperature signal is then scaled accordingly, posted to the HMI screen, and compared to the appropriate temperature set point. If the controls and alarms are enabled, appropriate modifications are made to the temperature control signal, and alarm indications.

    Chamber Temperature (Deg F) Control:

    Sampling Method:The temperature transducer is inserted directly into the isolator chamber. Unless otherwise capped off the temperature sensor is continuously exposed to the isolator atmosphere. When RH sensing is present on the isolator, the temperature sensor is typically combined with this sensor. If there is no RH sensing present then an independent temperature-sensing probe is used. Without RH sensing present, the sensing transducer is typically an industrial RTD. With RH sensing present the sensing transducer is typically an integrated chip (IC).

    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is a numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's RH Set Point (RH SP).
    • Process Variable (PV):  The RH transducer continuously senses the chamber RH level. The transducer converts and transmits the RH signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's RH Process Variable (RH PV).

    Chamber Vaporized Hydrogen Peroxide (VHP) Level

    Sampling Methods: Sampling pumps are used to pull a small portion of the isolator's atmosphere past the VHP sensor(s). The sampled atmosphere is pumped through .250"OD X .125" ID VITON or TEFLON tubing passed by the VHP sensor and returned to the chamber.  The sample pump(s) are configured to run based on different the different conditional requirements for each phase of operation.

    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's VHP Set Point (VHP SP).
    • Process Variable (PV):  The VHP transducer continuously senses the VHP level present in the sampling system. The transducer converts and transmits the VHP signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the chamber's VHP Process Variable (VHP PV).

     

    Chamber HEPA Pressure Level

    Sampling Method: Chamber inlet and exhaust HEPA filter pressure is sampled using  .250"OD X .125" ID polyurethane tubing. The pressure transducers are continuously exposed to chamber atmosphere.

    • One pressure port on the each respective pressure transducer will be terminated to a dedicated pressure sense port on the "non-chamber side" of the inlet or exhaust port HEPA filter. The second pressure sense port on each respective transducer will be tee'd together and terminated to a dedicated pressure port penetrating the isolator chamber. This tubing method generates a differential pressure measurement acrossed the HEPA relative to chamber pressure.
    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into a Phase Parameter Screen accessible from the HMI. The set point variable associated with the HEPA filter pressures used only for alarm functions.
    • Process Variable (PV):An appropriately ranged pressure transducer senses the resulting differential pressure. The transducer converts and transmits the air pressure signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is  the HEPA filter Differential Pressure Process Variable (HEPA DP PV).

    Control and Indications Variables

    As stated above control function associated with HEPA filter pressure variables is limited to alarm. 

    Chamber Vapor Level

    • Sampling Methods: The vapor sensor is directly located within the isolator chamber.  Unless otherwise capped off, the transducer is exposed to, and sampling, the chamber atmosphere at all times.
    • Set Point Variable (SPV): The operator is responsible for entry of the set point variable. This variable is the numeric value entered into the Phase Parameter Screen at the HMI. This value is considered the chamber's Vapor Set Point (VPR SP).
    • Process Variable (PV):  The vapor transducer continuously senses the vapor level. The transducer converts and transmits the vapor signal to an electrical signal. The electrical signal format used is typically a 4-20ma analog signal.  The 4-20ma signal is then routed to an analog input, at the PLC, where the program then translates the electrical signal to a raw decimal value. This raw decimal value is considered the Vapor Process Variable (VPR PV).
    • Control  and Indication Variables (CV): The program will scale the process variable to appropriate units of vapor (typically % vapor). Each individual operating phase will use the scaled PV & SP values for enabling control logic & outputs to associated devices, numeric or graphical displays at the HMI, and conditional logic for alarms

    Specifications:

    • Chamber Exterior Size: Length x Width x Height: Approx. 60”L X 26” W X 30” H
    • Overall Equipment Size: Length x Width x Height: Approx. 65”L X 33” W X 86” H
    • Shell: 316 L S.S
    • Stand: 304 S.S
    • Finishing: 304 S.S
    • Windows: Glass windows
    • HVAC Piping: 316 L S.S
    • Filters: HEPA
    • Gas: CDA(type of gas); regulator;
    • Humidity chamber: monitor
    • Utility Panel Compression Fitting: 2, teflon, 3”/8” Compression
    • Power: 125V, 60 Hz
    • Compressed air : Yes
    • Pressure: 90 Psi
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    Wave Biotech BASE 2050EH Bioreactor

    Wave Biotech BASE 2050EH Bioreactor

    Cambridge Scientific ID #'s: 13042, 17883

    Features:

    • Disposable Presterile Bioreactor Chamber
    • Fit Cellbags: 2x2L, 2x10L, 1x20L with KIT20 or 2x22L, 1X50L with KIT50
    • Completely closed system
    • Operate inside an incubator or on the benchtop

     

    Includes:

    • Electric Rocker Base for 20/50EHT
    • Digital temperature control

     

    Applications:

     

    Monoclonal antibodies

    The WAVE Bioreactor system has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scale up without transfers. Batches ranging from 100ml to 500L have been run with cell densities over 10x106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.

     

    Anchorage-dependent cells

    Agitation in the WAVE Bioreactor system is powerful enough to mix and aeratethe culture. Yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents setting and provides oxygenation without bubbles.

     

    Virus production

    The WAVE Bioreactor system provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4x106 cells/ml and virus production was 1x105 virus particles/cell. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet.

     

    Insect cell / Baculovirus

    The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.

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    Wave-Biotech-BASE-2050EH-Bioreactor-13042
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