Used Lab Equipment

Specifications:

• Capacity: 2.6 cu. Ft. (75 L)
• Max. temperature: 135°C
• Flask capacity: 12
• Included baskets: 32
• Dimensions: 38” x 18.8” x 24.9” ( H x W x D)

Comes with computer loaded w/ Unicorn Software

The purification of biological therapeutics requires processes which usually employ two technologies used in combination: membrane separations and chromatography. Chromatography offers greater selectivity than membrane separations, and can purify much greater quantities of solution in one operation.

However, membrane separations technologies offer a number of other advantages including the fact that they are a fast and robust process step.

ÄKTAcrossflow covers three main filtration techniques:

• Ultrafiltration of proteins
• Diafiltration of proteins
• Microfiltration of cell and lysate solutions

The system supports both hollow fibers and flat sheet cassettes with sizes suitable for flow rates up to 300 ml/min for flat sheet cassettes and up to 600 ml/ min for hollow fibers.

The main application is method development and scale-up in process development laboratories.

Specifications:

• Main Voltage: 100 to 240 V~ ±10%, 50–60Hz (Auto range)
• Weight: 70 kg
• Recirculation Volume Min.: 25 ml (with 1.7 mm i.d. tubing kit)
• Width: 620 mm
• Application: Well-suited for process development, filter screening, and process optimization at small scale.
• Compliance: The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from GE Healthcare and connected only to other CE labeled GE Healthcare modules or other products as recommended. For regulatory details please see declaration of conformity.
• Flow Cell Path Length: 2 mm
• Connections: UNF 5/16"
• Wavelength [Zn Lamp]: 214 nm
• Hold-Up Volume Recirculation Line: < 20 ml (with 1.7 mm i.d. tubing kit, empty reservoir)
• Length: 400 mm
• Operating Pressure Max.:2 bar (75.4 psi)
• Material Compliance: USP Class V
• Height: 650 mm
• Protection Class: IP 20
• Complete Packsize: 1 Piece
• pH Range: 2 - 12
• Mixing Principle: Magnetic stirrer

Features:

AKTAmicro, including BOX900, PH/C-900. UV-900,P-905

Micro Purification and purity analysis of biomolecules at submicrogram levels

Specifications:

• Voltage: 100-240V
• Frequency: 50-60Hz
• Max Power: 600 VA
• Fuse: T 6,3AL 250V
• Lamp intensity:210nm 69% 300nm 70%

AKTAprime plus is a compact chromatography system designed for one-step purification of proteins at laboratory scale. AKTAprime plus offers significant advantages in terms of speed, capacity and fraction collection.  AKTA PRIME is available only as a complete system with the following built-in functions: Gradient Pump, Motorized 8-port buffer selection valve, motorized injection valve, monitor, fraction collector.

Features:

• Fraction collector
• pH Electrode
• UV Monitor
• Computer loaded w/ Primeview Software, Monitor Keyboard & Mouse 

Purification can be performed at microgram, milligram or gram scale, depending on the core system and kits selected.

Features:

• Box 900: Storage compartment and Solvent Tray
• CU-950: Communications Module
• P-950 sample pump
• P-900: System Pump Model with Four Pump Heads in Two Pump Modules
• M-925 Mixer: All eluents can be mixed with a high degree of accuracy an reproducibility.
• UV-900: Variable, Multiwavelength UV-Vis detector
• pH/C-900: pH and Conductivity Detector
• Frac-920: Fraction Collector
• Model INV-907: Injection Valve
• Model PV-908: Sample Outlet Valve, For Sample Collection, with 8 ports
• Computer loaded w/ Unicorn Version. 5.31 Software, Monitor Keyboard & Mouse 

Features:

• Simple column selection with huge built-in library
• Automatic titration of buffers across a broad pH range
• Quick and easy transfer of methods to production-scale
• UNICORN method wizard High performance purification and characterization
• State-of-the-art computer-controlled pumps, UV/conductance monitors, and fraction collector
• UV-Vis detection of up to three wavelengths simultaneous

Specifications:

• Flow rate: 0.01-100 ml/min
• Operating pressure max.: 10 MPa
• Tubing i.d. (flow path): 0.75 mm
• UV wavelength: 190-700 nm
• Flow cell path length: 2 mm
• pH monitoring: 2–12
• Buffer selection: 11 buffer inlets
• Relative humidity: 10%-95% (non condensing)
• pH Stability: 1-14
• Viscosity: max 5 cP
• Multiple wavelength detection: Yes, up to 3 wavelengths
• Multiple sample injection: Yes, up to 8 samples
• Autosampler injection: Optional
• Automatic buffer preparation: BufferPrep
• Pump type: Piston pump, metering type
• Application: Purification of microgram to gram quantities of protein
• Dimensions (W x H x D): 480 x 610 x 470 mm
• Power Requirements: 100-120/220-240 VAC, 600W

Features:

The ImageQuant LAS 4000 is a camera system for producing digital images of gel or membrane samples or films. It is used together with the ImageQuant LAS 4000 Control Software to:

• Expose chemiluminescent samples or membranes.

• Expose fluorescent samples using epi (incident) or trans (transmitted) light sources, for example gels stained by ethidium bromide (EtBr). The ImageQuant LAS 4000 can be supplied with NIR, Red, Green, Blue and UV epi lights.

• Expose dye-stained gels and membranes or films using epi or trans illumination.

• Expose a sample repeatedly, incrementally or by programming exposure schemes.

Specifications:

• CCD: 3 200 000 pixels (Fujifilm super CCD
• Cooling temperature: -25°C
• Exposure time: 1/100 seconds to 2 hours (Images can be continuously exposed for up to 30 hours using ImageQuant LAS 4000 Control Software.)
• Lens: High-sensitivity lens F0.85 43 mm, Wide view lens F1.8 24 mm
• Number of gradations:

Recorded image: 16 bit

Focusing: 8 bit

Specifications:

• Max. voltage: 3500 V (dc), ± 1750 V floating output with reference to Ground
• Max. power: 100 W
• Max. pressure cooling plate: 0.35 bar
• Dimensions: 16 x 31 x 40 cm
• Chemical resistance: The wetted parts are resistant to solvents commonly used in electrophoresis and solutions containing inorganic and organic acids, alkalis and alcohols.

The Typhoon instrument is a variable-mode imager that produces digital images of radioactive, fluorescent, or chemiluminescent samples. The Typhoon instrument is available in several models. The Typhoon Trio instrument contains three internal lasers (blue, green, and red).

Includes:

• Red, Blue, Green Laser Modules Built in
• Image Quant and Scan Control Software loaded on computer
• All Necessary Cables, Cords and Manuals

For DNA, RNA, and protein samples, users can choose from:

• Storage phosphor autoradiography
• Direct green (532 nm) excited fluorescence
• Direct red (633 nm) excited fluorescence
• Direct blue (488 nm) excited fluorescence
• Chemiluminescence

Features:

• When one of the five scanning modes is selected, the appropriate optical components are automatically activated.
• Typhoon imagers scan mounted and unmounted storage phosphor screens, gels and blots up to 35 x 43 cm as well as microarrays.
• Typhoon imagers exhibit outstanding linearity and quantitative  accuracy, and include ImageQuant TL Image Analysis Software.
• Typhoon imagers are fully optimized as part of the Ettan DIGE system, and seamless integration with DeCyder Differential Analysis Software
• is ensured for all models.
• Special tray templates that are part of the latest scanner control software and new optional Gel Alignment Guides add higher throughput and ease of handling 2-D gels run on Ettan DALT and SE600.

The Typhoon instrument is a variable-mode imager that produces digital images of radioactive, fluorescent, or chemiluminescent samples. The Typhoon instrument is available in several models. The Typhoon Trio instrument contains three internal lasers (blue, green, and red).

Includes:

• Red, Blue, Green Laser Modules Built in
• Image Quant and Scan Control Software loaded on computer
• All Necessary Cables, Cords and Manuals

For DNA, RNA, and protein samples, users can choose from:

• Storage phosphor autoradiography
• Direct green (532 nm) excited fluorescence
• Direct red (633 nm) excited fluorescence
• Direct blue (488 nm) excited fluorescence
• Chemiluminescence

Features:

• When one of the five scanning modes is selected, the appropriate optical components are automatically activated.
• Typhoon imagers scan mounted and unmounted storage phosphor screens, gels and blots up to 35 x 43 cm as well as microarrays.
• Typhoon imagers exhibit outstanding linearity and quantitative  accuracy, and include ImageQuant TL Image Analysis Software.
• Typhoon imagers are fully optimized as part of the Ettan DIGE system, and seamless integration with DeCyder Differential Analysis Software
• is ensured for all models.
• Special tray templates that are part of the latest scanner control software and new optional Gel Alignment Guides add higher throughput and ease of handling 2-D gels run on Ettan DALT and SE600.

Description:

The Ettan IPGphor II from GE Healthcare is the ideal option for obtaining perfectly resolved first dimensions of 2D gels. The manufacturer claims that the system provides ‘reproducibility, reliability, speed, accuracy, high protein loading capacity, and high throughput’, and in my experience, the system really stands up to their claim. To begin with, the system is very compact and can be housed on a tabletop in any corner of the lab. It is perfectly silent and occupies little space. The system requires an ambient, dust-free operating environment; however, we keep the instrument in a well air-conditioned room. The system contains gold-plated copper electrodes that provide the necessary contact to the 1D strips for carrying out isoelectric focusing. A very high voltage is applied between the electrodes and, therefore, caution should be used while handling the instrument. As a safety feature, the electrodes are automatically switched off when anyone opens the lid of the instrument.