Used Lab Equipment

Specifications:

• Total volume: up to 20 L 
• Minimum working volume: 0.1 L 
• Maximum working volume: 10 L 
• Temperature range: Ambient to 40°C

Features:

• Disposable Presterile Bioreactor Chamber
• Fit Cellbags: 2x2L, 2x10L, 1x20L with KIT20 or 2x22L, 1X50L with KIT50
• Completely closed system
• Operate inside an incubator or on the benchtop

Includes:

• Electric Rocker Base for 20/50EHT
• Digital temperature control

Applications:

Monoclonal antibodies

The WAVE Bioreactor system has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scale up without transfers. Batches ranging from 100ml to 500L have been run with cell densities over 10x106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.

Anchorage-dependent cells

Agitation in the WAVE Bioreactor system is powerful enough to mix and aeratethe culture. Yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents setting and provides oxygenation without bubbles.

Virus production

The WAVE Bioreactor system provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4x106 cells/ml and virus production was 1x105 virus particles/cell. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet.

Insect cell / Baculovirus

The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.

Comes with computer loaded w/ Unicorn Software

The purification of biological therapeutics requires processes which usually employ two technologies used in combination: membrane separations and chromatography. Chromatography offers greater selectivity than membrane separations, and can purify much greater quantities of solution in one operation.

However, membrane separations technologies offer a number of other advantages including the fact that they are a fast and robust process step.

ÄKTAcrossflow covers three main filtration techniques:

• Ultrafiltration of proteins
• Diafiltration of proteins
• Microfiltration of cell and lysate solutions

The system supports both hollow fibers and flat sheet cassettes with sizes suitable for flow rates up to 300 ml/min for flat sheet cassettes and up to 600 ml/ min for hollow fibers.

The main application is method development and scale-up in process development laboratories.

Specifications:

• Main Voltage: 100 to 240 V~ ±10%, 50–60Hz (Auto range)
• Weight: 70 kg
• Recirculation Volume Min.: 25 ml (with 1.7 mm i.d. tubing kit)
• Width: 620 mm
• Application: Well-suited for process development, filter screening, and process optimization at small scale.
• Compliance: The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from GE Healthcare and connected only to other CE labeled GE Healthcare modules or other products as recommended. For regulatory details please see declaration of conformity.
• Flow Cell Path Length: 2 mm
• Connections: UNF 5/16"
• Wavelength [Zn Lamp]: 214 nm
• Hold-Up Volume Recirculation Line: < 20 ml (with 1.7 mm i.d. tubing kit, empty reservoir)
• Length: 400 mm
• Operating Pressure Max.:2 bar (75.4 psi)
• Material Compliance: USP Class V
• Height: 650 mm
• Protection Class: IP 20
• Complete Packsize: 1 Piece
• pH Range: 2 - 12
• Mixing Principle: Magnetic stirrer

Features:

• Disposable Presterile Bioreactor Chamber
• Completely closed system
• Operate inside an incubator or on the benchtop

Includes:

• Electric Rocker Base for 20
• Digital temperature control

Applications:

Monoclonal antibodies

The WAVE Bioreactor system has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scale up without transfers. Batches ranging from 100ml to 500L have been run with cell densities over 10x106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.

Anchorage-dependent cells

Agitation in the WAVE Bioreactor system is powerful enough to mix and aeratethe culture. Yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents setting and provides oxygenation without bubbles.

Virus production

The WAVE Bioreactor system provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4x106 cells/ml and virus production was 1x105 virus particles/cell. The WAVE Bioreactor system produces viruses under complete containment without the need for biosafety cabinet.

Insect cell / Baculovirus

The high oxygen supply capability of the WAVE Bioreactor system makes it ideal for insect cell culture.Cell densities over 9x106 are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor system is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.