Used Lab Equipment

Features:

The IQ5 system offers an affordable option for the detection of common green fluorescent dyes such as FAM and SYBR Green I. It offers five-target analysis capabilities for multiple PCR with a variety of detection chemistries.

Specifications:

• Temperature: 4 -100 C
• Accuracy: +- 0.3 C of programmed target
• Uniformity: +- 0.4 C well-to-well
• Fluorescence excitation range: 515-700 nm
• Sensitivity: One copy of 1L-1B in human genomic DNA
• Compatible dyes: FAM,SYBR GREEN I, HEX, TAMRA,TEXAS RED,Cy5
• Dimensions：29.2x58.4x38.7(11.5x23x15.2’’_
• Weight: 17.6kg (39lb)

Features:

• Computer w/ MxPro QPCR Software
• Four channels with customizable filters for multiplex up to 4 simultaneous targets
• Open platform supports all fluorescent chemistries
• 96 Well Block

The Mx3000P real-time PCR system is a fully integrated real-time PCR detection system. The system includes a state-of-the-art thermal cycler, a quartz-tungsten halogen lamp to excite fluorescence, a photomultiplier tube for high-sensitivity detection, and real-time quantitative detection and analysis software. With the Mx3000P real-time PCR system, monitoring PCR in real time is fast, easy, and reliable. The Mx3000P system has an open format that allows closed-tube real-time PCR detection with many detection chemistries including SYBR® Green dye and fluorogenic probe systems. The Mx3000P system supports both real-time and plate-read experiments.

The software features a variety of specific experiment types with customized plate setup, thermal profile setup, and analysis screens that streamline the process of collecting and analyzing data for specific applications. Some of the features that differentiate the Mx3000P real-time PCR system from other real-time detection instruments include the following:

• View and analyze data in multiple formats, including: amplification plots, scatter plots, sample value screens that display all dyes for the entire plate, fluorescence intensity screens, final call results, melting curves, annealing range and text reports
• Enhanced analysis algorithms, including adaptive baseline, to improve results and reduce the need for manual adjustments
• Melting curve analysis for Molecular Beacon confirmation with automatic melting temperature (Tm) calculation
• Ability to plot individual dyes and wells from an experiment without exporting data
• Real-time amplification plots can be viewed and results analyzed during PCR run
• All results available as raw data

Features:

• Computer w/ MxPro QPCR Software
• Four channels with customizable filters for multiplex up to 4 simultaneous targets
• Open platform supports all fluorescent chemistries
• 96 Well Block

The Mx3000P real-time PCR system is a fully integrated real-time PCR detection system. The system includes a state-of-the-art thermal cycler, a quartz-tungsten halogen lamp to excite fluorescence, a photomultiplier tube for high-sensitivity detection, and real-time quantitative detection and analysis software. With the Mx3000P real-time PCR system, monitoring PCR in real time is fast, easy, and reliable. The Mx3000P system has an open format that allows closed-tube real-time PCR detection with many detection chemistries including SYBR® Green dye and fluorogenic probe systems. The Mx3000P system supports both real-time and plate-read experiments.

The software features a variety of specific experiment types with customized plate setup, thermal profile setup, and analysis screens that streamline the process of collecting and analyzing data for specific applications. Some of the features that differentiate the Mx3000P real-time PCR system from other real-time detection instruments include the following:

• View and analyze data in multiple formats, including: amplification plots, scatter plots, sample value screens that display all dyes for the entire plate, fluorescence intensity screens, final call results, melting curves, annealing range and text reports
• Enhanced analysis algorithms, including adaptive baseline, to improve results and reduce the need for manual adjustments
• Melting curve analysis for Molecular Beacon confirmation with automatic melting temperature (Tm) calculation
• Ability to plot individual dyes and wells from an experiment without exporting data
• Real-time amplification plots can be viewed and results analyzed during PCR run
• All results available as raw data

The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. Offering unmatched flexibility and reliability, the systems are ideal for a wide variety of applications and chemistries (including but not limited to: gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis and chromatin immune-precipitation studies.

Highly reproducible results are the product of the Mx3005P’s single light source, single detector precision optic scanning design, providing uniform excitation and detection, coupled with the trusted Peltier-based thermal system, which ensures uniform ramping and thermal accuracy.

Features:

• Computer w/ MxPro QPCR Software
• Four channels with customizable filters for multiplex up to 4 simultaneous targets
• Open platform supports all fluorescent chemistries
• 96 Well Block

Instrument Specifications

• Excitation source: Quartz Tungsten Halogen lamp
• Detector: 1 scanning photomultiplier tube (PMT)
• Multiplexing capabilities: 5 targets in the same tube
• Thermal system: solid-state, Peltier-based
• Embedded computer safeguards data
• Tube/plate formats: 200 µL , 96-well format, optimized for 25 µL sample size
• Filters: Dual customizable filter wheels with four user-selected filters*
• Dynamic Range: 10 orders of magnitude
• Excitation Range: 350nm-750nm
• Emission Range: 350nm-700nm
• Temperature Uniformity: +/- .25°C at 72ËšC
• Temperature Ramp Rate: up to 2.5°C/second
• Temperature Range: 25-99°C
• Dimensions: 14" (35.6 cm) W x 20" (50.8 cm) D x 18" (45.7 cm) H, weight: 45 lbs (20.4 kg)
• Standard Filters
  • FAM/SYBR Green 1
  • HEX/JOE/VIC
  • ROX/Texas Red
  • Cy5
  • Cy3

Includes:

• Computer
• Software
• All Necessary Cables
• Manuals

Quantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables.

The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses.

The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction.

Applications

• DNA target quantification
• Gene expression
• SNP detection, Allele discrimination, GenotypingDNA methylation, Apoptosis
• Viral load assays, Pathogen detection
• Clinical diagnosis (Cancer therapy response)

Includes Computer loaded w/ Step One Software

The StepOnePlus Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Features of the StepOnePlus Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays
• Sensitive 4-color optical LED recording system
• Intuitive and robust software perfect for both first-time and advanced users
• Simple and flexible instrument set-up and usage

Specifications:

• Capacity: 1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes
• Detection Method: Primer-Probe Detection, SYBR
• Dimensions: 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
• Dynamic Range: Linear Dynamic Range greater than 9 log units (detection)
• Format: 0.1 ml tubes, 8-tube strips, 96-well plate
• Green Features: Energy efficient, Fewer resources used, Sustainable disposal
• High Throughput Compatibility: Multiplexing
• Optics: 4 emission filters, Blue LED excitation source, photodiode
• Passive Reference Dye: No ROX, ROX (Pre-mixed), ROX (Separate Tube)
• Peak Block Ramp Rate: 4.6°C⁄sec
• Reaction Speed: Fast, Standard
• Reaction Volume Range: 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
• Run Time: <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
• Sample Ramp Rate: Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
• Temperature Accuracy: 0.25°C (35 to 95°C) of display temperature
• Temperature Range (Metric): 4-100°C
• Temperature Uniformity: 0.25°C (35 to 95°C) of setpoint/display temperature
• Thermal Cycling System: Peltier-Based System
• Weight: 24 kg (53 lbs.)

Features:

Rapid high-throughput, plate-based real-time PCR amplification and detection instrument.

Specifications:

• Reaction Vessel: 96-well PCR plate, 384-well PCR plate
• Reaction Volume: 96-well PCR plate: 10 - 100 μL; 384-well PCR plate: 5 - 20 μL
• Excitation Source: Broad-spectrum, high-intensity LED (390 – 710 nm)
• Detection: CCD camera
• Detection Filters: 488, 510, 580, 610,640, 660 nm (System II)
• Measuring Mode: Real-time, online (continuous display of readings)
• Temperature Control: Peltier-based heating/cooling
• Run Time: <40 minutes (384-well PCR plate)

This LCMS brings you the best in quantitative sensitivity and robustness to expand upon the experiments going beyond MRM, and give you substantial improvements in data quality.

Includes:

• AB Sciex 4500 LC/MS/MS
• Shimadzu Prominence 20 UFLC  
• Shimadzu DGU-20A3 Degasser
• (2)Shimadzu LC_20AD Liquid Chromatograph
• Shimadzu CTO-20AC Column Oven
• Shimadzu SIL-20AC UFLC
• Windows 7 Computer Loaded with Analyst 1.6 Software       

Specifications:

• Sensitivity MRM: mode Reserpine 200 fg on column S/N > 2000  C.V. < 5%
• Maximum scan speed: 12,000 Da/sec
• Polarity switching time: 50 msec, in MRM and scheduled MRM (sMRM) modes
• Minimum MRM dwell time: 1 msec
• Mass range (m/z): 5-2000
• Cross talk for reserpine 609/195: <0.5% cross talk detectable with 2 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion MS for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), and scheduled MRM (sMRM)
• Detector type: AcQuRate Pulse Counting CEM
• Dynamic range: 4 orders of magnitude
• Ionization sources: Turbo V source housing with TurboIonSpray probe or APCI probe.
• Maximum temperature: 750ºC
• Flow rate compatibility: 5 μL/minute to 3 mL/minute
• Power: 208V 50/60Hz AB Sciex 4500, 208V Vacuum Pump, 208V Peak N2 Generator, 115V Shimadzu UPLC
• Dimensions: 37" x 33" x 55" Sciex 4500
• Weight: 250lbs

 Includes One Year Service Contract, PM at 6 Months, and Installation

The AB SCIEX QTRAP 5500 System is a high sensitivity, bench top hybrid triple quadrupole-Linear Accelerator trap mass spectrometer designed for LC/MS/MS analyses. This instrument provides an uncompromised combination of Linear Accelerator trap and triple quadrupole functionality, uniquely allowing both  qualitative and quantitative analyses to be carried out in a single experiment.

Includes:

• AB Sciex 5500 Qtrap LC/MS/MS
• Agilent 1200 HPLC System
  
  • Agilent G1322A Degasser
  • Agilent G1311A Quat Pump
  • Agilent G1315D DAD (Diode Array Detector)
• Solvent Tray
• Computer Loaded with Analyst 1.6 Software       

Specifications

• Sensitivity MRM mode Reserpine: 50 fg on column S/N > 2000
• V.: < 5%
• Triple quad scan speed: 12,000 Da/sec
• Linear ion trap scan speed: 20,000 Da/sec
• Polarity switching time: 50 msec
• Minimum MRM Dwell Time: 1 msec
• Triple quad mass range (m/z): 5-1250
• Linear ion trap mass range (m/z): 50-1000
• Cross talk for Reserpine 609/195: No significant cross talk detectable with 1 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), enhanced MS scan, enhanced product ion scan, enhanced resolution scan, MS  scan, MRM3 scan, and TripleTrap scan modes

Includes

• Gene Pulser II
• Capacitance Extender Plus
• Pulse Controller Plus

Includes Installation and One year Service Contract

Includes:

• G1946D LC/MSD Mass Spectrometer Series SL
• Roughing Pump
• Optional Front end HPLC

Specifications

• Mass Range: 2-3000m/z
• Mass Accuracy: +/-.13 u within the calibrated mass range in scan mode
• Mass Axis Stability: +- 0.13 u over 8 hours
• Scan Speed:
  • 2500 u/s standard mode
  • 5250 u/s fast scan mode
• SIM Sensitivity:
  • ESI at 400 ul/min
  • APCI at 1000 ul/min
  • Selected ion monitoring of m/z 609.3
  • Positive ionization
  • Signal To Noise Ratio
  • Quantity: 1 pg reserpine 100:1 RMS (20:1 peak-to-peak)
• Scan Sensitivity:
  • ESI at 400 ul/min
  • APCI at 1000 ul/min
  • Scan range m/z 100-650
  • Scan speed 2500 u/s
  • Extracted ion at m/z 609.3
  • Positive ionization
  • Signal To Noise Ratio
  • Quantity: 50 pg reserpine 100:1 RMS (20:1 peak-to-peak)
• Multiple Signal Acquisition: Ability to cycle through four different acquisition modes on a scan-by-scan basis within a single run

Optional Installation, Familiarization, and Extended Warranty available

Includes:

• G1322 Degasser
• G1311 Quarternary Pump or G1312A Binary Pump($1000 Less)
• G1313A Autosampler
• G1316A Column Compartment
• G1315A Diode Array Detector
• Computer Loaded with ChemStation
• Monitor – Mouse – Keyboard
• Cables and Manuals

The components can be customized to your needs.

Please let me know if I can be of any additional assistance.

Includes: 

• Qty (2) - G1361A Agilent 1100 Series Prep Pumps
• G1315A DAD Diode Array Detector (G1314A VWD Detector also available)
• G2258A Agilent 1100 Prep Autosampler
• G1364B AFC Automated Fraction Collector
• Computer Loaded with ChemStation
• Monitor – Mouse – Keyboard
• Cables and Manuals

Includes:

• Agilent G1322A Degasser
• Agilent G1311A Quat Pump
• Agilent G1329A Autosampler
• Agilent G1330A Autosampler Chiller
• Agilent G1365D DAD (Diode Array Detector)
• Agilent G1316A Column Compartment
• Solvent Tray
• Computer Loaded with ChemStation
• Cables and Manuals

Features:

This Agilent 1220 Infinity LC - G4294B has a Gradient Pump, Autosampler, Column oven and Diode Array Detector configuration. It also includes Agilent Instrument Utilities Software.

Specifications:

• Weight: 43 kg (94 lbs)

• Dimensions (height × width× depth): 640×370×485 mm (25.2×14.6×19.1 inches)

• Line frequency:  50 or 60 Hz, ± 5 %

• Power consumption: 240 VA / 210 W / 717 BTU

• Ambient operating temperature: 4–55 °C (39–131 °F)

• Ambient non-operating temperature: -40 – 70 °C (-40 – 158 °F)

• Humidity: < 95 % r.h. at 40 °C (104 °F)