Used Lab Equipment

Features:

• Computer w/ MxPro QPCR Software
• Four channels with customizable filters for multiplex up to 4 simultaneous targets
• Open platform supports all fluorescent chemistries
• 96 Well Block

The Mx3000P real-time PCR system is a fully integrated real-time PCR detection system. The system includes a state-of-the-art thermal cycler, a quartz-tungsten halogen lamp to excite fluorescence, a photomultiplier tube for high-sensitivity detection, and real-time quantitative detection and analysis software. With the Mx3000P real-time PCR system, monitoring PCR in real time is fast, easy, and reliable. The Mx3000P system has an open format that allows closed-tube real-time PCR detection with many detection chemistries including SYBR® Green dye and fluorogenic probe systems. The Mx3000P system supports both real-time and plate-read experiments.

The software features a variety of specific experiment types with customized plate setup, thermal profile setup, and analysis screens that streamline the process of collecting and analyzing data for specific applications. Some of the features that differentiate the Mx3000P real-time PCR system from other real-time detection instruments include the following:

• View and analyze data in multiple formats, including: amplification plots, scatter plots, sample value screens that display all dyes for the entire plate, fluorescence intensity screens, final call results, melting curves, annealing range and text reports
• Enhanced analysis algorithms, including adaptive baseline, to improve results and reduce the need for manual adjustments
• Melting curve analysis for Molecular Beacon confirmation with automatic melting temperature (Tm) calculation
• Ability to plot individual dyes and wells from an experiment without exporting data
• Real-time amplification plots can be viewed and results analyzed during PCR run
• All results available as raw data

Includes Computer loaded w/ Step One Software

The StepOnePlus Real-Time PCR System is a 96-well Real-Time PCR instrument perfect for both first-time and experienced users. The StepOnePlus Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 4-color optical recording, the StepOnePlus Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.

Features of the StepOnePlus Real-Time PCR System include:

• Advanced software and instrumentation for performing a wide array of genomic assays
• Sensitive 4-color optical LED recording system
• Intuitive and robust software perfect for both first-time and advanced users
• Simple and flexible instrument set-up and usage

Specifications:

• Capacity: 1 x 96-well plate, 12 x 8-tube strips, 96 x 0.1 ml tubes
• Detection Method: Primer-Probe Detection, SYBR
• Dimensions: 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H)
• Dynamic Range: Linear Dynamic Range greater than 9 log units (detection)
• Format: 0.1 ml tubes, 8-tube strips, 96-well plate
• Green Features: Energy efficient, Fewer resources used, Sustainable disposal
• High Throughput Compatibility: Multiplexing
• Optics: 4 emission filters, Blue LED excitation source, photodiode
• Passive Reference Dye: No ROX, ROX (Pre-mixed), ROX (Separate Tube)
• Peak Block Ramp Rate: 4.6°C⁄sec
• Reaction Speed: Fast, Standard
• Reaction Volume Range: 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated)
• Run Time: <2 hrs⁄run (Standard Mode), <40 min⁄run (Fast Mode)
• Sample Ramp Rate: Fast mode: ± 2.2°C⁄sec, Standard mode: ± 1.6°C⁄sec
• Temperature Accuracy: 0.25°C (35 to 95°C) of display temperature
• Temperature Range (Metric): 4-100°C
• Temperature Uniformity: 0.25°C (35 to 95°C) of setpoint/display temperature
• Thermal Cycling System: Peltier-Based System
• Weight: 24 kg (53 lbs.)

Features:

• QPCR: UF-150 GENECHECKER Ultra-Fast Real-time PCR System

Specifications:

• Operating Mechanism : Precise Control of Peltier Element

• Temperature Accuracy : ± 0.5°C 

• Temperature Uniformity : ± 0.5°C (Well to Well)

• Temperature Stability : ± 0.5°C 

• Ramping Rate : 8.0°C / second 

• Temperature Range : 35 ~ 65  (1.0°C Increment) for RT Step   20 ~ 99  (1.0°C Increment) for PCR 

• Sample Format : Polymer Based 3-Dimensional Microfluidic Chip

• Number of Samples per Run : 10 

• Required Sample Volume : 10μl 

• Typical PCR Duration : Less than 12 Minutes for 30 Cycles (without RT Step)

• Method of Detection : Comparison of Digitized Fluorescence

• Signal Display : Text LCD (4 Line) 

• Integrated Memory : Saves up to 12 PCR Protocols  

• Type of Excitation : High Brightness LED 

• Wavelength : 465nm ± 10nm  

• Number of Detection Channel : 1 

• Method of Fluorescence Measurement : Integrated Camera Module 

• PC Connection : USB 2.0 A to B (PC to Device, Only available through GENERECORDER)

• Power (Wattage) : AC 110-230V (50-60Hz) Input / DC 12V Output (70W) 

• Dimension / Weight : 200(w) x 200(d) x 125(h) mm / 3.2kg

The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan® Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability.

• Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate
• Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction
• Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences
• 96- or 384-well plate compatibility (including the TaqMan® Low Density Array)
• Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security
• Hands-free plate-loading and unloading provides true walkaway automation allowing you to increase your lab's productivity
• Proven assay development guidelines save time and money

Specifications:

• Calibrated Dye: SYBR® Green I, ROX™, NED™, JOE™, TAMRA™, VIC™, FAM™, TET™
• Capacity: Up to 84 x 384-well plates with optional automation accessory
• Detection Method: SYBR, Primer-Probe Detection
• Dimensions: 72 cm (W) x 84 cm (D) x 64 cm (H) (with drawer open)
• High-throughput Compatibility: High-throughput Compatible, Multiplexing
• Optics: Extended-life 488 nm argon-ion laser excitation source, No filters (CCD acts as spectrograph with continuous detection 500 -650 nm)
• Passive Reference Dye: ROX (Separate Tube), No ROX, Other Passive Reference Dye, ROX (Pre-mixed)
• Reaction Speed: Fast 
• Reaction Volume Range: 5-20 µl
• Run Time: 52 minutes (384-well in fast mode), <2 hrs (384-well in standard mode)
• Sample Ramp Rate: Fast mode: ± 3.0°C⁄sec, Standard mode: ± 1.6°C⁄sec
• Temperature Accuracy: ±0.25°C of display temperature
• Temperature Range (Metric): 4-100°C
• Temperature Uniformity: ±0.5°C
• Thermal Cycling System: Peltier-Based System
• Throughput: >5,000 real-time PCR rxns per day (384-well block and automation accessory), >10,000 endpoint PCR rxns per hour (384-well block and automation accessory)
• Weight: 114 kg (250 lbs) with automation accessory, 82 kg (180 lbs) without automation accessory
• Block Format: Interchangeable
• Dynamic Range: Up to 9 logs of linear dynamic range
• Sensitivity: Down to 10 copies

Includes

• Gene Pulser II
• Capacitance Extender Plus
• Pulse Controller Plus

This LCMS brings you the best in quantitative sensitivity and robustness to expand upon the experiments going beyond MRM, and give you substantial improvements in data quality.

Includes:

• AB Sciex 4500 LC/MS/MS
• Shimadzu Prominence 20 UFLC  
• Shimadzu DGU-20A3 Degasser
• (2)Shimadzu LC_20AD Liquid Chromatograph
• Shimadzu CTO-20AC Column Oven
• Shimadzu SIL-20AC UFLC
• Windows 7 Computer Loaded with Analyst 1.6 Software       

Specifications:

• Sensitivity MRM: mode Reserpine 200 fg on column S/N > 2000  C.V. < 5%
• Maximum scan speed: 12,000 Da/sec
• Polarity switching time: 50 msec, in MRM and scheduled MRM (sMRM) modes
• Minimum MRM dwell time: 1 msec
• Mass range (m/z): 5-2000
• Cross talk for reserpine 609/195: <0.5% cross talk detectable with 2 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion MS for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), and scheduled MRM (sMRM)
• Detector type: AcQuRate Pulse Counting CEM
• Dynamic range: 4 orders of magnitude
• Ionization sources: Turbo V source housing with TurboIonSpray probe or APCI probe.
• Maximum temperature: 750ºC
• Flow rate compatibility: 5 μL/minute to 3 mL/minute
• Power: 208V 50/60Hz AB Sciex 4500, 208V Vacuum Pump, 208V Peak N2 Generator, 115V Shimadzu UPLC
• Dimensions: 37" x 33" x 55" Sciex 4500
• Weight: 250lbs

 Includes One Year Service Contract, PM at 6 Months, and Installation

The AB SCIEX QTRAP 5500 System is a high sensitivity, bench top hybrid triple quadrupole-Linear Accelerator trap mass spectrometer designed for LC/MS/MS analyses. This instrument provides an uncompromised combination of Linear Accelerator trap and triple quadrupole functionality, uniquely allowing both  qualitative and quantitative analyses to be carried out in a single experiment.

Includes:

• AB Sciex 5500 Qtrap LC/MS/MS
• Agilent 1200 HPLC System
  
  • Agilent G1322A Degasser
  • Agilent G1311A Quat Pump
  • Agilent G1315D DAD (Diode Array Detector)
• Solvent Tray
• Computer Loaded with Analyst 1.6 Software       

Specifications

• Sensitivity MRM mode Reserpine: 50 fg on column S/N > 2000
• V.: < 5%
• Triple quad scan speed: 12,000 Da/sec
• Linear ion trap scan speed: 20,000 Da/sec
• Polarity switching time: 50 msec
• Minimum MRM Dwell Time: 1 msec
• Triple quad mass range (m/z): 5-1250
• Linear ion trap mass range (m/z): 50-1000
• Cross talk for Reserpine 609/195: No significant cross talk detectable with 1 msec dwell time and 3 msec inter-MRM pause time
• Triple quadrupole mass stability: 0.1 Da over 24 hours
• Scan types: Full scan MS and selected ion for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), enhanced MS scan, enhanced product ion scan, enhanced resolution scan, MS  scan, MRM3 scan, and TripleTrap scan modes

Optional Installation, Familiarization, and Extended Warranty available

Includes:

• G1322 Degasser
• G1311 Quarternary Pump or G1312A Binary Pump($1000 Less)
• G1313A Autosampler
• G1316A Column Compartment
• G1315A Diode Array Detector
• Computer Loaded with ChemStation
• Monitor – Mouse – Keyboard
• Cables and Manuals

The components can be customized to your needs.

Please let me know if I can be of any additional assistance.

Includes Computer loaded w/ Image Lab Software

The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications.

Its features are based on CCD high-resolution, high-sensitivity detection technology, and modular options to accommodate a wide range of samples and support multiple detection methods. The system is controlled by Image Lab software to optimize performance for fast, integrated, and automated image capture and analysis of various samples.

Features and Benefits

• Multiple imaging capabilities — the ChemiDoc MP imager can accommodate a variety of sample types and detection methods including multiplex fluorescent western blotting. It is the perfect imager to accompany your protein and DNA electrophoresis runs as well as your western blotting experiments. It delivers quantitative, reproducible results for fluorescence, chemiluminescence, and colorimetric detection
• Stain-free technology — UV-induced fluorescence labeling of proteins in the stain-free gels allows a 2 hr Coomassie gel-staining protocol to be condensed into a 5 min stain-and-image step. Stain-free gels are western blot compatible — using the V3 Western Workflow™, check your electrophoresis results and blot transfer quality prior to western blotting
• High-sensitivity blot detection — the ChemiDoc MP imaging system offers advanced detection technology that determines optimal exposure, even for faint or intense samples. Superior sensitivity is achieved for chemiluminescence and multiplex fluorescence detection and for colorimetric gel and blot documentation
• Superior Image Quality — exceptional dynamic range enables visualization of faint and intense bands on same blot or gel. Images are always in focus at any zoom level to ensure publication-ready images in seconds
• Ease of Use — precalibrated system provides the precise focus for any zoom setting or sample height; automated hands-free operation ensures consistent, reproducible, and high-throughput performance

Applications     

• Chemiluminescence: Yes
• Fluorescence: Yes
• Colorimetry/densitometry: Yes
• Gel documentation:Yes

Hardware Specifications        

• Maximum sample size: 28 x 36 cm
• Maximum image area: 26 x 35 cm
• Maximum image area: (For standard UV-excited gels) 25 x 26 cm
• Excitation source: Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available); optional trans-white conversion screen, XcitaBlue™ conversion screen, and epi-red, epi-green, and epi-blue LED modules available
• Illumination control: 8 modes available. Trans-UV, epi-white, and no illumination for chemiluminescence are standard; epi-red, epi-green, epi-blue, trans-white, and XcitaBlue conversion screens are optional
• Detector: Supercooled CCD
• Image resolution: 4 megapixels
• Pixel size (H x V): 6.45 x 6.45 µm
• Cooling system: Peltier
• Camera cooling temperature: -30°C absolute and regulated
• Filter holder: 6 positions (5 for filters, 1 without filter for chemiluminescence)
• Emission filters: 1 included (standard), 4 optional
• Dynamic range: >4.0 orders of magnitude
• Pixel density (gray levels): 65,535
• Dynamic flat fielding: Application specific; for all applications
• Instrument size (L x W x H): 36 x 60 x 96 cm
• Instrument weight: 32 kg

Operating Ranges       

• Operating voltage: 110/115/230 VAC nominal
• Operating temperature: 10–28°C (21°C recommended)
• Operating humidity, noncondensing: <70%

Automation Capabilities         

• Workflow automated selection: Application driven; user selected or recalled by a protocol
• Workflow automated execution: Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
• Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
• Autofocus (patent pending): Precalibrated focus for any zoom setting or sample height
• Image flat fielding**: Dynamic; precalibrated and optimizor every application
• Autoexposure: 2 user-defined modes (intense or faint bands)

Features: 

Features for the Photometrics Cool SNAP K4: 

• 20-MHz readout: High-speed, high-sensitivity image capture
• 2048 x 2048 imaging array: Resolves fine detail
• 7.4 x 7.4-ìm pixels: Ideally matched to optical microscope
• Interline-transfer, progressive-scan CCD: Eliminates camera vibration and facilitates fast triggering
• Flexible binning and readout: Increases light sensitivity while increasing the frame rate
• 12-bit digitization: Quantifies bright and dim signals in the same image
• Thermoelectric cooling: Long integration times for higher sensitivity
• Enhanced quantum efficiency: Provides higher sensitivity than typical interline cameras
• C-mount: Easily attaches to microscopes, standard lenses, or optical equipment
• Acquisition software: Captures, analyzes, and saves high-resolution images
• PCI interface: High-bandwidth, uninterrupted data transfer
• PVCam, Circular buffers, Device sequencing: Supported by numerous third-party software packages, Real-time focus, Precise integration with shutters, filter wheels, etc. (Compatible with Windows XP/Vista 32, Mac OS X, and Linux (kernel versions 2.4 and 2.6.8))
• CCD image sensor: Kodak KAI-4020M; interline-transfer, progressive-scan device with microlenses
• CCD format: 2048 x 2048 imaging array, 7.4 x 7.4-um pixels, 15.16 x 15.16-mm imaging area (optically centered)
• Linear full well: 30,000 e- (single pixel); 60,000 e- (2 x 2 binned pixel)
• Read noise: ≤10 e- rms @ 20 MHz
• Nonlinearity: <1%
• Digitizer type: 12 bits @ 20 MHz
• CCD temperature: -25ͦC (regulated)
• Dark current : 0.1 e-/p/s @ -25ͦC
• Operating environment: 0 to 30ͦC ambient, 0 to 80% relative humidity noncondensing
• I/O TTL (trigger/status): trigger, invert, inhibit, exposing, interline shift, frame readout, 8-bit TTL (programmable), 8-bit DACs(two)

Compare this against “Features of Gel Doc XRS+ system”: 

• Chemiluminescence: Yes
• Fluorescence: Yes
• Colorimetry/densitometry: Yes
• Gel documentation: Yes  

Hardware Specifications        

• Maximum sample size: 28 x 36 cm
• Maximum image area: 26X 35 cm
• Excitation source: Trans-UV and epi-white are standard (302 nm included, with 254 and 365 nm available as options)
• Illumination control: 3 modes (trans-UV, trans white, epi-white)
• Detector: super cooled CCD
• Image resolution: 4 megapixels
• Pixel size (H x V): 6.45 x 6.45 µm
• Filter holder: 3 positions (2 for filters, 1 without filter)
• Emission filters: 1 included (standard), 3 optional
• Dynamic range: >4.0 orders of magnitude
• Pixel density (gray levels): 65,535
• Dynamic flat fielding: Application specific, for all applications
• Instrument size (L x W x H): 36 x 60 x 96 cm
• Instrument weight: 32 kg

 Operating Ranges         

• Operating voltage: 110/115/230 V AC nominal
• Operating temperature: 10–28°C (21°C recommended)
• Operating humidity: <70% noncondensing

 Automation Capabilities            

• Workflow automated selection: Application driven; user selected or recalled by a protocol
• Workflow automated execution: Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
• Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
• Autofocus: Precalibrated focus for any zoom setting or sample height
• Image flat fielding: Dynamic; precalibrated and optimized per application
• Autoexposure: 2 user-defined modes (intense or faint bands)

Includes:

• G2445C Mass Spectrometer Series SL
• G1972A Mass Tech Ion Source
• Roughing Pump
• Optional HPLC Front end
• Computer with Chemstation Software