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Used Lab Equipment

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Bruker AMX-300 NMR System

Bruker AMX-300 NMR System

Cambridge ID #: 18992

Bruker AMX-300 Nuclear Magnetic Resonance Spectrometer with the "piano" style console and an unshielded Oxford 300MHz magnet. The magnet was de-energized about five years ago. All of the kits for moving the magnet are included.  

This is a 2-channel system with a solids accessory rack with high power 1H and X transmitters. Three probes included (plus a Doty solid state probe): a 5mm 1H inverse probe, a 10mm BB, and a standard bore magic angle solids probe (7 & 4mm stators).  

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Bruker-AMX-300-NMR-System-18992
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Applied Biosystems API 3000 LC/MS/MS  System

Applied Biosystems API 3000 LC/MS/MS System

Cambridge ID #: 8337

includes 1 year Service Contract, Installation, and a PM at 6 months

Method Development and Training also Available

 

The API 3000 LC/MS/MS System is an enhanced high performance triple quadrupole mass spectrometer with applications in protein and peptide analysis, small molecule analysis in drug metabolism and pharmacokinetic studies, and n-in-one cassette dosing analysis.

 

Includes:

  • API 3000Mass Spectrometer, MS/MS System
  • Optional Front end HPLC
  • Mass Range: 50-2250da
  • Windows 7 Computer loaded w/ Analyst Software 1.6
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Applied-Biosystems-API-3000-LC-MS-MS--System-8337
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AB Sciex API 3200 Mass Spectrometer w/ Shimadzu Prominence LC-20AD UFLC and CTC Autosampler LC/MS/MS  System

AB Sciex API 3200 Mass Spectrometer w/ Shimadzu Prominence LC-20AD UFLC and CTC Autosampler LC/MS/MS System

Cambridge ID #: 14837

Includes Installation and One Year Warranty (Preventative Maintenance at 6 months)

Method Development and Training also Available

 

Includes:

  • AB Sciex API 3200 Mass Spectrometer LC/MS/MS
  • Windows 7 Computer loaded w/ Analyst Software 1.5
  • Turbo V-Spray ESI
  • Vacuum pump
  • Shimadzu Prominence 20 UFLC  Shimadzu
  • Shimadzu DGU-20A3 Degasser
  • (2)Shimadzu LC_20AD Liquid Chromatograph
  • Shimadzu CTO Column Oven
  • Shimadzu CBM-20A Communication Module
  • CTC Autosampler
  • Monitor – Mouse – Keyboard
  • Manuals


The API 3200 LC/MS/MS system is a fully-integrated triple quadrupole mass spectrometer built using premier technology that combines sensitivity and selectivity with ruggedness and reliability, all in an affordable, compact package

  • Innovative Turbo V ion source efficiently ionizes compounds over a wide range of flow rates, virtually eliminating cross-contamination, even with large sample loads
  • Patented LINAC collision cell technology delivers superior MS/MS performance allowing faster scan times without compromising sensitivity or mass spectral quality
  • Simplifies cross-platform methods transfer
  • Gives meaningful results from every experiment

Specifications:

  • Detector type: Pulse Counting CEM
  • Dynamic Range: 5 orders of magnitude
  • Ionization Sources: TurboIonSpray, APCI, DuoSpray, PhotoSpray
  • Mass Accuracy / Stability: 0.1 Da over 8 hours and 0.15 Da over 24 hours
  • Mass Range: 5-1,700 (Quad); 50-1,700 (LIT)
  • Polarity Switching Time: 700 ms
  • Scan Speed (Quad): 24,000 Da/sec
  • Scan Types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM, EMS, EPI, ER, MS3, TripleTrap
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AB-Sciex-API-3200-Mass-Spectrometer-w--Shimadzu-Prominence-LC-20AD-UFLC-and-CTC-Autosampler-LC-MS-MS--System-14837
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AB Sciex API 4000 Qtrap w/ Shimadzu 20AD UPLC LC/MS/MS Q Trap System

AB Sciex API 4000 Qtrap w/ Shimadzu 20AD UPLC LC/MS/MS Q Trap System

Cambridge Scientific ID #'s: 13945, 20542

Includes 1 year Service Contract, Installation, and a PM at 6 months

Method Development and Training also Available

 

Includes:

  • AB Sciex API4000 LC/MS/MS
  • Enhanced high performance triple quadrupole mass spectrometer system
  • Line transformers
  • Turbo V Ion Source (with APCI and ESI probes)
  • Roughing Pump
  • Windows 7 Computer loaded w/ Analyst Software 1.6
  • Cart
  • Shimadzu Prominence 20 UFLC  Shimadzu
    • Shimadzu DGU-20A3 Degasser
    • (2)Shimadzu LC_20AD Liquid Chromatograph
    • Shimadzu CTO Column Oven
    • Shimadzu CBM-20A Communication Module
  • CTC Autosampler
  • Monitor – Mouse – Keyboard
  • Cables and Manuals

 

Specifications:

  • Detector type: Pulse Counting CEM
  • Dynamic Range: 5 orders of magnitude
  • Ionization Sources: ESI, APCI
  • Mass Accuracy / Stability: 0.1 Da over 8 hours, 0.15 Da over 24 hours
  • Mass Range: 5-3,000
  • Polarity Switching Time: 700 ms
  • Scan Speed (Quad): 24,000 Da/sec
  • Scan Types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
  • Sensitivity MRM Mode: 200 fg Reserpine on column: S/N > 1,200, C.V. < 5%
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AB-Sciex-API-4000-Qtrap-w--Shimadzu-20AD-UPLC-LC-MS-MS-Q-Trap-System-13945
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AB Sciex API 4000 w/ Agilent 1100 HPLC and CTC Autosampler LC/MS/MS  System

AB Sciex API 4000 w/ Agilent 1100 HPLC and CTC Autosampler LC/MS/MS System

Cambridge ID #: 14794

Includes 1 year Service Contract, Installation, and a PM at 6 months

Includes:

  • AB Sciex API4000 LC/MS/MS
  • Enhanced high performance triple quadrupole mass spectrometer system
  • Line transformers
  • Turbo V Ion Source (with APCI and ESI probes)
  • Roughing Pump
  • Windows 7 Computer loaded w/ Analyst Software 1.6
  • Cart
  • Agilent 1100 HPLC and CTC Autosampler
    • G1322 Degasser
    • G1312A Binary Pump
    • G1316A Column Compartment
    • G1323A Game Boy
    • CTC Autosampler
  • Monitor – Mouse – Keyboard
  • Cables and Manuals

 

Specifications:

  • Detector type: Pulse Counting CEM
  • Dynamic Range: 5 orders of magnitude
  • Ionization Sources: ESI, APCI
  • Mass Accuracy / Stability: 0.1 Da over 8 hours, 0.15 Da over 24 hours
  • Mass Range: 5-3,000
  • Polarity Switching Time: 700 ms
  • Scan Speed (Quad): 24,000 Da/sec
  • Scan Types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
  • Sensitivity MRM Mode: 200 fg Reserpine on column: S/N > 1,200, C.V. < 5%
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AB-Sciex-API-4000-w--Agilent-1100-HPLC-and-CTC-Autosampler-LC-MS-MS--System-14794
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AB Sciex API 4000 w/ Shimadzu 20AC UPLC LC/MS/MS  System

AB Sciex API 4000 w/ Shimadzu 20AC UPLC LC/MS/MS System

Cambridge ID #: 18606

Includes:

  • AB Sciex API4000 LC/MS/MS
  • Enhanced high performance triple quadrupole mass spectrometer system
  • Line transformers
  • Turbo V Ion Source (with HESI probes)
  • Roughing Pump
  • Windows 7 Computer loaded w/ Analyst Software 1.6
  • Cart
  • Optional Shimadzu Front End
  • Monitor – Mouse – Keyboard
  • Cables and Manuals

Specifications:

  • Detector type: Pulse Counting CEM
  • Dynamic Range: 5 orders of magnitude
  • Ionization Sources: ESI, APCI
  • Mass Accuracy / Stability: 0.1 Da over 8 hours, 0.15 Da over 24 hours
  • Mass Range: 5-3,000
  • Polarity Switching Time: 700 ms
  • Scan Speed (Quad): 24,000 Da/sec
  • Scan Types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
  • Sensitivity MRM Mode: 200 fg Reserpine on column: S/N > 1,200, C.V. < 5%

Optional Shimadzu UFLC System Components:

  • SIL-20AC
  • LC-20AB
  • CTO-20AC
  • CBM-20A
  • DGU-20A5
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AB-Sciex-API-4000-w--Shimadzu-20AC-UPLC-LC-MS-MS--System-18606
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AB Sciex API 5000 LC/MS/MS Mass Spectrometer w/ Agilent 1100 HPLC and CTC Autosampler LC/MS/MS  System

AB Sciex API 5000 LC/MS/MS Mass Spectrometer w/ Agilent 1100 HPLC and CTC Autosampler LC/MS/MS System

Cambridge ID #: 15382

Innovative QJet ion guide technology dramatically improves sensitivity by capturing and focusing more ions generated by the efficient TURBO V ion source. And the TURBO V source virtually eliminates cross-contamination, even with high-concentration samples.  New innovations in sensitivity like QJet ion guide technology make it possible to reach the lowest limits of detection. Combining QJet ion guide technology with the proven TURBO V ion source and a powerful new generation of Analyst software results in a robust, high throughput platform ideal for all phases of pharmaceutical development.

 

Includes:

  • AB Sciex API5000 LC/MS/MS
  • Enhanced high performance triple quadrupole mass spectrometer system
  • Line transformers
  • Turbo V Ion Source (with APCI and ESI probes)
  • Roughing Pump
  • Windows 7 Computer loaded w/ Analyst Software 1.6
  • Cart
  • Agilent 1100 HPLC and CTC Autosampler
    • G1322 Degasser
    • G1312A Binary Pump
    • G1316A Column Compartment
    • G1323A Game Boy
    • CTC Autosampler
  • Monitor – Mouse – Keyboard
  • Cables and Manuals

 

Specifications:

  • Detector type: Pulse-counting channel electron multiplier (CEM) detector
  • Dynamic Range: 1 cps to 4 x 10(6) cps (pulse counting)
  • Ionization Sources: ESI, APCI
  • Mass Accuracy / Stability: 0.01% over the entire mass range
  • Mass Range: 5 – 1250 m/z
  • Scan Types:  Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
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AB-Sciex-API-5000-LC-MS-MS-Mass-Spectrometer-w--Agilent-1100-HPLC-and-CTC-Autosampler-LC-MS-MS--System-15382
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AB Sciex API 5500 Qtrap w/ Shimadzu Prominence LC-20AD UFLC and CTC Autosampler LC/MS/MS Q Trap System

AB Sciex API 5500 Qtrap w/ Shimadzu Prominence LC-20AD UFLC and CTC Autosampler LC/MS/MS Q Trap System

Cambridge ID #: 14839

Includes One Year Service Contract, PM at 6 Months, and Installation

 

The AB SCIEX QTRAP 5500 System is a high sensitivity, bench top hybrid triple quadrupole-Linear Accelerator trap mass spectrometer designed for LC/MS/MS analyses. This instrument provides an uncompromised combination of Linear Accelerator trap and triple quadrupole functionality, uniquely allowing both  qualitative and quantitative analyses to be carried out in a single experiment.

 

Includes:

  • AB Sciex 5500 Qtrap
  • Shimadzu Prominence 20 UFLC  Shimadzu
    • Shimadzu DGU-20A3 Degasser
    • (2)Shimadzu LC_20AD Liquid Chromatograph
    • Shimadzu CTO Column Oven
    • Shimadzu CBM-20A Communication Module
    • CTC Autosampler
    • Computer Loaded with Analyst 1.6 Software       

Specifications

  • Sensitivity MRM mode Reserpine: 50 fg on column S/N > 2000
  • V.: < 5%
  • Triple quad scan speed: 12,000 Da/sec
  • Linear ion trap scan speed: 20,000 Da/sec
  • Polarity switching time: 50 msec
  • Minimum MRM Dwell Time: 1 msec
  • Triple quad mass range (m/z): 5-1250
  • Linear ion trap mass range (m/z): 50-1000
  • Cross talk for Reserpine 609/195: No significant cross talk detectable with 1 msec dwell time and 3 msec inter-MRM pause time
  • Triple quadrupole mass stability: 0.1 Da over 24 hours
  • Scan types: Full scan MS and selected ion for both Q1 and Q3, product ion scan, precursor ion scan, neutral loss or gain scan, multiple reaction monitoring (MRM), enhanced MS scan, enhanced product ion scan, enhanced resolution scan, MS  scan, MRM3 scan, and TripleTrap™ scan modes
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AB-Sciex-API-5500-Qtrap-w--Shimadzu-Prominence-LC-20AD-UFLC-and-CTC-Autosampler-LC-MS-MS-Q-Trap-System-14839
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Illumina BeadXpress Laser Detection Bioassay SystemImage Coming Soon

Illumina BeadXpress Laser Detection Bioassay System

Cambridge ID #: 9551

Description:

Illumina’s BeadXpress Reader was specifically developed to analyze bioassay applications using the versatile VeraCode technology. With the broadest multiplexing range in the industry, the BeadXpress Reader is the optimal solution for small and high-throughput laboratories conducting molecular testing applications that benefit from robust, multiplexed-based assays. VeraCode technology presents a digital revolution for multiplex testing in genotyping, gene expression, and protein-based assays. The unique holographic code embedded in each VeraCode bead type delivers unparalleled data quality by enabling customizable tracking and providing an unprecedented level of data precision and verifiability.

Specifications:

  • Altitude: up to 2400 m
  • Temperature range: 15°C to 30°C
  • Humidity: 20 to 80% RH
  • Electrical Ratings: 90–260 V (AC), 2.0 A, 50/60 Hz, Class I
  • Dimensions (W x D x H): 47 cm x 77 cm x 62 cm
  • Weight: 57 kg
  • Laser Classification: Class I laser instrument housing two internal Class 3B lasers (IEC 60825-1, 1.2, 1993)
  • Power: Dedicated circuit; BeadXpress Reader (110V): 15 A; BeadXpress Reader (220V): 12 A

 

 

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Illumina-BeadXpress-Laser-Detection-Bioassay-System-9551
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Europlasma CD600 Nanofics S/SE Nano Coating System

Europlasma CD600 Nanofics S/SE Nano Coating System

Cambridge Scientific ID #'s: 21201, 21202

Specifications:

Process chamber

  • Material: aluminium
  • Inside dimensions: 900 x 570 x 550 mm
  • Volume: 282 liter
  • Tray: 3, with dimensions 765 x 512 mm or custom design

Vacuum measurement: Baratron gauge

Generator: Custom design for Nanofics application

Pumping system: Dry pump group

PLC

The equipment and automatic processing is fully controlled by an industrial PLC.

The HMI (user interface) runs over an industrial touch panel PC having following properties:

  • 17” touch screen.
  • LED backlight.
  • Integrated SSD hard drive.

The HMI offers following functionalities:

  • Process & Instrumentation Diagram showing real-time machine status.
  • User-level based log on for managing user rights from operator to engineer mode.
  • Recipe management system offering unlimited number of recipes, each recipe representing a unique set of process parameters.
  • Diagnostic functions.
  • Alarm management.
  • Optional historical data logging and viewing.

Electricity: 380V AC, 50Hz, 40A

Process gas

  • 2 gas lines with MFC (Mass Flow Controller) for S coating
  • 3 gas lines with MFC (Mass Flow Controller) for SE coating

Safety features: Emergency-off-switch, vacuum interlock, over temperature interlock, CE-label

Process gas connection: ¼’’-Swagelok-fitting, input pressure 1 Bar

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Europlasma-CD600-Nanofics-S-SE-Nano-Coating-System-21201
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Thermo Cellomics CellInsight Gel Imaging SystemImage Coming Soon

Thermo Cellomics CellInsight Gel Imaging System

Cambridge ID #: 10067

Description:

High-content screening (HCS) or high-content analysis (HCA) requires an instrument designed to extract the maximum information from your sample in a robust and reproducible manner with the speed for high-throughput analysis. The entire Thermo Scientific portfolio of HCA instruments helps deliver on those parameters and includes:

  • Superior illumination—the LED light engine reduces intensity fluctuations and optical component wear, eliminates moving parts, and reduces both integration and channel switching times
  • Rapid data analysis—Thermo Scientific HCS Studio software analyzes your images in real time, so that you collect all the data you need to make decisions that count
  • Reagent compatibility—Thermo Scientific HCS platforms build on the expertise of Molecular Probes in reagent development with exquisite fluorescent tools for interrogating the biology of the cell

Specifications:

  • Camera: Photometrics X1 Camera with 4.54um/pixel
  • Live cell: Optional HCA On-Stage Incubator with Kinetic scheduling of acquisition
  • Software: HCA Studio 3.1 Cell Analysis Software. Optional client software available
  • Database: STORE Express Image and Database Management Software Included, Scalable SQL/Oracle database options available
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Bio-Rad CHEF-DR II Gel Electrophoresis System

Bio-Rad CHEF-DR II Gel Electrophoresis System

Cambridge ID #: 13111

Includes:

  • Cooling Module
  • Control Module
  • Drive Module

 

Drive Module 

  • Dimensions (W x D x H): 43 x 26 x 14 cm
  • Construction: Aluminum chassis
  • Weight: 8 kg (17.2 lb)
  • Maximum voltage:250 V DC
  • Maximum current: 500 mA
  • Electrode potentials:Dynamically regulated (feedback adjustment) +/- 0.5%
  • Input voltage range:
    • 90–132 VAC/47–63 Hz/0.7 A (maximum)
    • 198–264 VAC/47–63 Hz/0.35 A (maximum)
  • Fuses:
    • 5 A Fast Blow for high voltage output (front panel)
    • A Slow-Blow (100/120 V) or
    • 5 A Slow-Blow (220/240 V)

Control Module          

  • Dimensions (W x D x H): 31 x 13.5 x 17 cm
  • Maximum current: 500 mA
  • Allowable voltage gradients:6–6 V/cm, in 0.1 V/cm increments
  • Data entry: Keyboard
  • Power supply: 250 V maximum, to allow maximum gradient of 6 V/cm, continuously adjustable

Cooling Module

  • Chassis: Aluminum
  • Dimensions (W x L x H):23 x 42 x 24 cm
  • Weight: 14 kg (30.1 lb)
  • Compressor:1/12 HP
  • Refrigerant:CFC 12
  • Heat exchanger:Stainless steel
  • Operating range: 5°C–25°C
  • Cooling rate (pre-cool):75°C/min from ambient to 14°C
  • Cooling capacity (input power): 75 W at 14°C
  • Input voltage range       
    • 90–132 VAC/47–63 Hz/4 A
    • 198–264 VAC/47–63 Hz/2 A
  • Fuses   
  • 3 A Slow-Blow (100/120 V) or
  • 0 A Slow-Blow (220/240 V)
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Bio-Rad-CHEF-DR-II-Gel-Electrophoresis-System-13111
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Syngene Chemi Genius Gel Imaging System

Syngene Chemi Genius Gel Imaging System

Cambridge ID #: 20230

Features:

 

Chemi Genius 2 Bio Imaging System

 

Specifications:

  • Hamamatsu C4742-80-12AG ORCA-ER Camera Controller

 

  • Hermetic Vacuum Sealed Head
  • High Resolution of 1.37 Million Pixels
  • Exposure Time up to 10 Seconds
  • Progressive Scan Interline CCD Chip with No Mechanical Shutter
  • Binning Function

 



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Alpha Innotech Chemi-Imager Gel Imaging SystemImage Coming Soon

Alpha Innotech Chemi-Imager Gel Imaging System

Cambridge Scientific ID #'s: 5918, 7150

Features

  • 20-MHz readout: High-speed, high-sensitivity image capture
  • 2048 x 2048 imaging array: Resolves fine detail
  • 7.4 x 7.4-ìm pixels: Ideally matched to optical microscope
  • Interline-transfer, progressive-scan CCD: Eliminates camera vibration and facilitates fast triggering
  • Flexible binning and readout: Increases light sensitivity while increasing the frame rate
  • 12-bit digitization: Quantifies bright and dim signals in the same image
  • Thermoelectric cooling: Long integration times for higher sensitivity
  • Enhanced quantum efficiency: Provides higher sensitivity than typical interline cameras
  • C-mount: Easily attaches to microscopes, standard lenses, or optical equipment
  • Acquisition software: Captures, analyzes, and saves high-resolution images
  • PCI interface: High-bandwidth, uninterrupted data transfer
  • PVCam, Circular buffers, Device sequencing: Supported by numerous third-party software packages, Real-time focus, Precise integration with shutters, filter wheels, etc. (Compatible with Windows XP/Vista 32, Mac OS X, and Linux (kernel versions 2.4 and 2.6.8))
  • CCD image sensor: Kodak KAI-4020M; interline-transfer, progressive-scan device with microlenses
  • CCD format: 2048 x 2048 imaging array, 7.4 x 7.4-um pixels, 15.16 x 15.16-mm imaging area (optically centered)
  • Linear full well: 30,000 e- (single pixel); 60,000 e- (2 x 2 binned pixel)
  • Read noise: ≤10 e- rms @ 20 MHz
  • Nonlinearity: <1%
  • Digitizer type: 12 bits @ 20 MHz
  • CCD temperature: -25ͦC (regulated)
  • Dark current : 0.1 e-/p/s @ -25ͦC
  • Operating environment: 0 to 30ͦC ambient, 0 to 80% relative humidity noncondensing
  • I/O TTL (trigger/status): trigger, invert, inhibit, exposing, interline shift, frame readout, 8-bit TTL (programmable), 8-bit DACs(two)

Compare this against “Features of Gel Doc XRS+ system”: 

  • Chemiluminescence: Yes
  • Fluorescence: Yes
  • Colorimetry/densitometry: Yes
  • Gel documentation: Yes  

Hardware Specifications        

  • Maximum sample size: 28 x 36 cm
  • Maximum image area: 26X 35 cm
  • Excitation source: Trans-UV and epi-white are standard (302 nm included, with 254 and 365 nm available as options)
  • Illumination control: 3 modes (trans-UV, trans white, epi-white)
  • Detector: super cooled CCD
  • Image resolution: 4 megapixels
  • Pixel size (H x V): 6.45 x 6.45 µm
  • Filter holder: 3 positions (2 for filters, 1 without filter)
  • Emission filters: 1 included (standard), 3 optional
  • Dynamic range: >4.0 orders of magnitude
  • Pixel density (gray levels): 65,535
  • Dynamic flat fielding: Application specific, for all applications
  • Instrument size (L x W x H): 36 x 60 x 96 cm
  • Instrument weight: 32 kg

 Operating Ranges         

  • Operating voltage: 110/115/230 V AC nominal
  • Operating temperature: 10–28°C (21°C recommended)
  • Operating humidity: <70% noncondensing

 Automation Capabilities            

  • Workflow automated selection: Application driven; user selected or recalled by a protocol
  • Workflow automated execution: Controlled by a protocol via application specific setup for image area, illumination source, filter, analysis, and reporting
  • Workflow reproducibility: 100% repeatability via recallable protocols; from image capture to quantitative analysis and reports
  • Autofocus: Precalibrated focus for any zoom setting or sample height
  • Image flat fielding: Dynamic; precalibrated and optimized per application
  • Autoexposure: 2 user-defined modes (intense or faint bands)

 

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Bio-Rad Chemidoc XRS Gel Imaging System

Bio-Rad Chemidoc XRS Gel Imaging System

Cambridge ID #: 14876

Includes:

  • Cabinet & Camera
  • Computer With Software
  • Manual

 

The Bio-Rad ChemiDoc XRS System is Bio-Rad’s most advanced imaging system. The system is able to image chemiluminescent, fluorescent and colorimetric samples and comes complete with a compact darkroom, transilluminator and supercooled high resolution 1.44 megapixel CCD camera. The entire system is controlled by the provided Quantity One 1-D analysis software with additional controls on the compact darkroom to control focus, aperture and zoom. Consequently, the ChemiDoc XRS system is extremely flexible and can be used for imaging a diverse range of applications such as Western blotting, DNA/RNA gel electrophoresis, 2-D gel electrophoresis, densitometry, etc. The system is able to detect HRP and AP chemiluminescence, ethidium bromide, SYBR Green, green fluorescent protein, silver stain and Coomasie brilliant blue staining to name but a few that are frequently used with the above applications. The system features flat fielding technology which allows for superior uniformity and quantification. Flat-fielding is a technique that corrects for pixel-to-pixel variations by dividing the gain variations out of an image, thus flattening the relative response for each pixel.

Operation of the system is very simple. The gel or membrane is placed on the transilluminator, which is set into a drawer which can be moved in and out of the darkroom, facilitating placement of the gel or membrane. Once the gel or membrane is placed, it and the transilluminator are returned to the darkroom and the door closed. With the Epi-illumination switched on, the aperture, zoom and focus are adjusted using the touchpad on the darkroom and viewed on the computer monitor until the desired position, size and focus of the gel are achieved. To acquire the image, the appropriate light source for the gel or membrane is selected and the software is used to select an integration time. Using the Quantity One software, the image is frozen when a satisfactory image is acquired. The Quantity One 1-D image analysis software aids the user in obtaining the best images possible from the gel or membrane. The software is user-friendly and equipped with built-in quick guides and wizards to facilitate the optimization of images and printouts. The software is compatible with both Microsoft Windows and Macintosh platforms. The Quantity One 1-D software is used for analysis of 1-D electrophoresis gels, Western blotting membranes, Coomassie-stained SDS-PAGE gels, etc. whereas the PDQuest 2-D analysis software is also available and is intended to be used for comparative analysis where many 2-D gels are analyzed at once.

 

Specifications:

  • CCD Resolution (HxV): 1,392 x 1,040µm
  • Pixel density: 65,536 gray levels
  • Imaging area: 25 x 26 cm
  • Camera cooling temperature: -50°C
  • Camera cooling system: Peltier
  • Dynamic range: 4 orders of magnitude
  • Motorized zoom lens: C-mount, f/1.2, 12-75 mm
  • Illumination modes: Epi-white, trans-UV (302 nm), and white
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