Used Lab Equipment

Used Lab Equipment

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Stratagene 400510 Transilluminator

Stratagene 400510 Transilluminator

Cambridge ID #: 14195

Transilluminators provide brilliant back illumination of transparent materials that are placed on the filter area. It is designed with a durable Plexiglass and glass filter combination and provides increased UV sensitivity and uniformity, while reducing electrical consumption. The transilluminators provide a highly sensitive method to detect double-stranded nucleic acids that have been labeled with fluorescent dyes such as ethidium bromide or acridine orange.


Specifications:

  • Filter Dimensions: 21 x 26 cm (8 1/4 x 10 1/4")
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Stratagene MX3000P Real-Time qPCR System

Stratagene MX3000P Real-Time qPCR System

Cambridge ID #: 18326

Features:

  • Computer w/ MxPro QPCR Software
  • Four channels with customizable filters for multiplex up to 4 simultaneous targets
  • Open platform supports all fluorescent chemistries
  • 96 Well Block

 

The Mx3000P real-time PCR system is a fully integrated real-time PCR detection system. The system includes a state-of-the-art thermal cycler, a quartz-tungsten halogen lamp to excite fluorescence, a photomultiplier tube for high-sensitivity detection, and real-time quantitative detection and analysis software. With the Mx3000P real-time PCR system, monitoring PCR in real time is fast, easy, and reliable. The Mx3000P system has an open format that allows closed-tube real-time PCR detection with many detection chemistries including SYBR® Green dye and fluorogenic probe systems. The Mx3000P system supports both real-time and plate-read experiments.

The software features a variety of specific experiment types with customized plate setup, thermal profile setup, and analysis screens that streamline the process of collecting and analyzing data for specific applications. Some of the features that differentiate the Mx3000P real-time PCR system from other real-time detection instruments include the following:

 

  • View and analyze data in multiple formats, including: amplification plots, scatter plots, sample value screens that display all dyes for the entire plate, fluorescence intensity screens, final call results, melting curves, annealing range and text reports
  • Enhanced analysis algorithms, including adaptive baseline, to improve results and reduce the need for manual adjustments
  • Melting curve analysis for Molecular Beacon confirmation with automatic melting temperature (Tm) calculation
  • Ability to plot individual dyes and wells from an experiment without exporting data
  • Real-time amplification plots can be viewed and results analyzed during PCR run
  • All results available as raw data
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Stratagene MX3005P Real-Time qPCR System

Stratagene MX3005P Real-Time qPCR System

Cambridge Scientific ID #'s: 19580, 13626

The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. Offering unmatched flexibility and reliability, the systems are ideal for a wide variety of applications and chemistries (including but not limited to: gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis and chromatin immune-precipitation studies.

 

Highly reproducible results are the product of the Mx3005P’s single light source, single detector precision optic scanning design, providing uniform excitation and detection, coupled with the trusted Peltier-based thermal system, which ensures uniform ramping and thermal accuracy.

 

Features:

  • Computer w/ MxPro QPCR Software
  • Four channels with customizable filters for multiplex up to 4 simultaneous targets
  • Open platform supports all fluorescent chemistries
  • 96 Well Block

 

Instrument Specifications

  • Excitation source: Quartz Tungsten Halogen lamp
  • Detector: 1 scanning photomultiplier tube (PMT)
  • Multiplexing capabilities: 5 targets in the same tube
  • Thermal system: solid-state, Peltier-based
  • Embedded computer safeguards data
  • Tube/plate formats: 200 µL , 96-well format, optimized for 25 µL sample size
  • Filters: Dual customizable filter wheels with four user-selected filters*
  • Dynamic Range: 10 orders of magnitude
  • Excitation Range: 350nm-750nm
  • Emission Range: 350nm-700nm
  • Temperature Uniformity: +/- .25°C at 72ËšC
  • Temperature Ramp Rate: up to 2.5°C/second
  • Temperature Range: 25-99°C
  • Dimensions: 14" (35.6 cm) W x 20" (50.8 cm) D x 18" (45.7 cm) H, weight: 45 lbs (20.4 kg)
  • Standard Filters
    • FAM/SYBR Green 1
    • HEX/JOE/VIC
    • ROX/Texas Red
    • Cy5
    • Cy3
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Stratagene MX4000 Real-Time qPCR System

Stratagene MX4000 Real-Time qPCR System

Cambridge ID #: 8298

Includes:

  • Computer
  • Software
  • All Necessary Cables
  • Manuals

 

Quantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables.

The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses.

The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction.

Applications

  • DNA target quantification
  • Gene expression
  • SNP detection, Allele discrimination, GenotypingDNA methylation, Apoptosis
  • Viral load assays, Pathogen detection
  • Clinical diagnosis (Cancer therapy response)
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Stratagene Picofige Microcentrifuge

Stratagene Picofige Microcentrifuge

Cambridge ID #: 17314

Specifications:

  • Rotation Speed: 6,400 rpm
  • G force: 2,000 rcf (xg)
  • Capacity: Holds 6 1.5-ml
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Stratagene UV Stratalinker 2400 UV Crosslinker

Stratagene UV Stratalinker 2400 UV Crosslinker

Cambridge ID #: 20447

Descriptions:

The Stratalinker UV crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. For optimal cross linking performance, each Stratalinker UV crosslinker is equipped with an internal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs.

 

Features:

  • 254-nm UV light bulbs, 15 Watts each
  • For Crosslinking DNA or RNA to Coated Glass Slides, Nylon, Nitrocellulose or Nylon-Reinforced Nitrocellulose Membranes
  • Greatly Reduces Valuable Time From Several Oven Baking Hours to 30-60 Seconds
  • Increases Hybridization Signal Compared to Oven Baking
  • Internal Photodetector Compensates for Shifting Output of Aging UV Bulbs
  • Can be Operated in Autocrosslink, Time or Energy Modes

 

Specifications:

 

  • External Dimensions: 58.9 W x 38.9 D x 26.7 H
  • Internal Dimensions: 47.7 W x 34.3 D x 16.8 H
  • Power Delivered: 4000 µW/cm²

 

  • Weight: 15.5 kg
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Stratagene White Light Box Transilluminator

Stratagene White Light Box Transilluminator

Cambridge ID #: 15211

Transilluminators provide brilliant back illumination of transparent materials that are placed on the filter area. It is designed with a durable Plexiglass and glass filter combination and provides increased UV sensitivity and uniformity, while reducing electrical consumption. The transilluminators provide a highly sensitive method to detect double-stranded nucleic acids that have been labeled with fluorescent dyes such as ethidium bromide or acridine orange.


Specifications:

  • Filter Dimensions: 21 x 26 cm (8 1/4 x 10 1/4")
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Stratagene-White-Light-Box-Transilluminator-15211
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