The Mx3000P real-time PCR system is a fully integrated real-time PCR detection system. The system includes a state-of-the-art thermal cycler, a quartz-tungsten halogen lamp to excite fluorescence, a photomultiplier tube for high-sensitivity detection, and real-time quantitative detection and analysis software. With the Mx3000P real-time PCR system, monitoring PCR in real time is fast, easy, and reliable. The Mx3000P system has an open format that allows closed-tube real-time PCR detection with many detection chemistries including SYBR® Green dye and fluorogenic probe systems. The Mx3000P system supports both real-time and plate-read experiments.
The software features a variety of specific experiment types with customized plate setup, thermal profile setup, and analysis screens that streamline the process of collecting and analyzing data for specific applications. Some of the features that differentiate the Mx3000P real-time PCR system from other real-time detection instruments include the following:
The Mx3005P system is among the most reliable and trusted QPCR instruments available, with a long record of citation in peer-review journals. Offering unmatched flexibility and reliability, the systems are ideal for a wide variety of applications and chemistries (including but not limited to: gene expression analysis, microarray data validation, SNP genotyping, pathogen detection, DNA methylation analysis and chromatin immune-precipitation studies.
Highly reproducible results are the product of the Mx3005P’s single light source, single detector precision optic scanning design, providing uniform excitation and detection, coupled with the trusted Peltier-based thermal system, which ensures uniform ramping and thermal accuracy.
Quantitative real-time PCR is a highly sensitive and specific fluorescence-based technique that measures the production of a target amplicon during the exponential phase of the polymerase chain reaction when PCR efficiency is least influenced by reaction variables.
The use of fluorogenic probes reduces the time to obtain results by eliminating post-PCR processing. Relative quantitation, based on expression of an internal housekeeping control, as well as absolute quantitation, based on standard curves of the target amplicon, can be achieved using real-time PCR methodology. Real-time QPCR has many other applications including allelic discrimination, viral load, cDNA microarray validation, gene dosage and SNP analyses.
The benefits of the automated MX4000 technology include real-time PCR detection; closed-tube assay, no electrophoresis, highly reproducible quantitative results, large dynamic range, 96 sample throughput and ability to analyse results during the reaction.
The Stratalinker UV crosslinker is designed to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crosslinking process takes only 25–50 seconds, in contrast to the traditional method of baking filters at 80°C for 2 hours. Additionally, crosslinking has been shown to significantly increase hybridization signals when compared to oven-baking. For optimal cross linking performance, each Stratalinker UV crosslinker is equipped with an internal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs.
Transilluminators provide brilliant back illumination of transparent materials that are placed on the filter area. It is designed with a durable Plexiglass and glass filter combination and provides increased UV sensitivity and uniformity, while reducing electrical consumption. The transilluminators provide a highly sensitive method to detect double-stranded nucleic acids that have been labeled with fluorescent dyes such as ethidium bromide or acridine orange.