Used Lab Equipment

Specifications:

• Number of sample plates: Total of two plates, expandable to up to 22 plates with optional Sample Organizer (see below):
  • 96 and 384 microtiter plates
  • 48 position 2.00-mL vial plates
  • 48 position 0.65-mL micro-centrifuge tube plates
  • 24 position 1.50-mL micro-centrifuge tube plates
• Maximum sample capacity: 768 in two 384-well plates; expandable to up to 8,448 samples with optional Sample Organizer
• Number of sample injections: 1 to 99 injections per sample
• Injection volume range: 0.1 to 50.0 iJL, in 0.1 iJL increments, partial or full loop mode; 10ul loop is standard; 1, 2, 5, 20, and 50-uL loops also available
• Sample delivery precision(full loop injection mode): < 0.3% RSO, full loop, standard lO-iJL loop (default wash/purge conditions), per Waters AQT/SystemsQT protocol
• Sample delivery precision(PLNO injection mode): < 1% RSO within 20% to 75% of loop volume for 1, 2, 5, 10, 20, and 50-iJL loops, UV detection
• Injector linearity: > 0.999 coefficient of deviation (from 20% to 75%, partial loop overfill mode (PLNO injection mode), per Waters' AQT/SystemsQT protocol
• Sample temperature control:0 to 40.0 ·C, settable in 0.1 ·C increments (assumes an ambient temperature of 25.0 ·C). At an ambient temperature of 21.0 ·C or lower the sample manager will maintain the temperature of the sample compartment down to 4.0 ·C with a tolerance of -2.0/+6.0 ·C, when configured with the maximum number of vials and/or plates
• Injection cycle time:
  • < 15 sec between multipe injections with "load ahead" enabled
  • 30 sec with single weak wash, lO-iJL loop, pressure assist mode
• Sample probe: XYZZ based needle-in-needle design
• Minimum sample required: 5 uL residual, using maximum recovery 2-mL vials (zero offset)
• Wash solvents: Two degassed: strong solvent and weak wash solvent, programmable to suit application
• Sample carryover: < 0.005% or < 2.000 nL, whichever is greater
• Advanced operations: Loop off-line mode, load ahead
• Unattended operation: Leak sensors, full diagnostic data control captured through console software
• Primary wetted material: Titanium alloy, 316 stainless steel, fluoropolymer, fluoroelastomer, PPS alloy, PEEK alloy, PPS, PEEK, OLC coating, gold

Specifications:

• Number of solvents: Up to four: in any combination of two: Al or A2 and Bl or B2
• Solvent storage: Solvents Tray accommodates up to four chromatographic solvents, two Sample Manager wash solvents and one Binary Solvent Manager seal wash solvent
• Solvent conditioning: Vacuum degassing (Six-channel): one channel per solvent, and two channels for Sample Manager wash solvents
• Operating flow rate range: 010 to 2.000 mL/min, in 0.001 mL increments
• Compressibility compensation: Automatic and continuous
• Effective system delay volume: <120 ulL, independent of system backpressure (with standard 50-ilL mixer installed)
• Plunger seal wash: Integral, active, programmable
• Gradient profiles: Eleven gradient curves [including linear, step (2), concave (4), and convex (4)]
• Wet prime: Automatic
• Maximum operating pressure: 15,000 psi up to 1 mL/min, 9,000 psi up to 2 mL/min per pump, not more than 15,000 psi total
• Composition accuracy:
  • ±0.5% absolute (full scale) from 5 to 95% of flow rates
  • from 0.5 to 2.0 mLlmin (Contact Waters for conditions used)
• Composition precision: 0.15% RSO or ±0.04 min SO, whichever is greater, based on retention time
• Flow precision: 0.075% RSO or ±0.02 min SO, six replicates, based on retention time or volumetric measures (0.500 to 2.000 ml/min)
• Flow accuracy: ± 1.0% at 0.5 mL/min with degassed methanol, per Waters AQT/SystemsQT protocol
• Primary wetted materials: 316 stainless steel, UHMWPE, sapphire, ruby, FEP, PTFE, ETFE, diamond-like coating, PEEK and PEEK alloys, titanium alloys
• Unattended operation: Leak sensors, full diagnostic data captured through console software

Specifications:

• Column capacity:
  • CM-A: Two columns, as standard (maximum length of 150 mm with filter or guard column)
  • or four columns (maximum length of 50 mm) can be supported with optional tubing kit,
  • up to 4.6 mm internal diameter (1.0.)
  • CM-Aux: Two columns (maximum length of 150 mm, with filter or guard column) - up to
  • two CM-Aux units can be configured with one CM-A for support of up to six columns
• Switching valves: Two nine-port, eight-position valves (CM-A only); provides programmable, automatic, random access switching, waste and bypass positions for rapid solvent changeover
• Temperature Range:
  • 0 to 90.0 ·C, set table in 0.1 ·C increments
  • Two independent heatl cool zones per module, up to six zones in stacked configuration
• Temperature Accuracy: ±0.5 ·C
• Temperature Stability: ±0.3 ·C
• Solvent conditioning: Active pre-heating as standard
• Column tracking: eCord Technology column information management tracks and archives column usage history
• 20 support: Optional

The ACQUITY UPLC Sample Organizer increases the level of automation and sample processing throughput of the ACQUITY UPLC System. It extends the capacity of the system by more than tenfold for samples in standard ANSI-compliant 96 or 384 well plates; or more than threefold for samples in conventional 2 mL vials.

Features:

• Plates are transferred in a programmable order from the Sample Organizer to the ACQUITY UPLC System Sample Manager for processing
• Plate exchange shuttle replaces a plate in the Sample Manager within 15 seconds to support high sample-throughput processing
• Shelves accessed through the large swing-open front door and slide in easily
• Accepts any combination of ANSI-compliant well plates and vial holders
• Temperature control of 4 to 40 °C in 1 °C increments
• Small footprint
• Sample capacities:
  • 22 standard ANSI/SBS-compliant 96 or 384 well plates
  • 12 intermediate well plates
  • 8 x 48 – 2mL vials
  • 8 x 24 – 4mL vials

Genevac designed the EZ-2^plus with the specific requirements of lead optimization in mind. Years of experience in solvent evaporation and sample purification allow Genevac to produce a high-efficiency sample concentrator that is high performance, easy to use, very reliable and compatible with almost all commonly used solvents and acids.

The EZ-2 Series is the culmination of an extensive research and development program that draws on the expertise of Genevac’s engineers and the experiences of many scientists in the laboratory. Using the most advanced  proven technology in evaporation science, the EZ-2 Series has been designed specifically for solvent removal in life science research, be that concentration of samples or complete drying. Genevac’s fifteen years of experience in vacuum engineering has resulted in the creation of a compact sample concentrator combining great performance, ease of use greater than a rotary evaporator and compatibility with all commonly used solvents and acids.

With a dedicated range of QIAsymphony Kits, the QIAsymphony AS/SP enables sample preparation of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials. The QIAsymphony AS extends the capabilities of the QIAsymphony SP by integrating automated PCR assay setup, which, in combination with the Rotor-Gene Q and QIAGEN real-time and end-point PCR kits, enables you to optimize your PCR workflow and maximize your efficiency.

Specifications:

• This system includes the Rotor-Gene Q
• Altitude: Up to 2000 m
• Elution Volume: 30 – 500 uL
• Humidity: 15-75% (relative, noncondensing), maximum 75%
• Input Volume: 200 uL
• Dimensions: 128 x 103 x 73 cm
• Operating Temperature: 15 - 32°C
• Power Requirements: 100-240V, 50/60Hz
• Storage Temperature: 5 - 40°C

The Waters ACQUITY UPLC H-Class System delivers the flexibility of quaternary solvent blending with the advanced performance of UPLC® separations. The system's holistic design is targeted for routine analysis and method development use and is perfectly suited for running both HPLC and UPLC applications while still realizing the improved resolution and sensitivity of UPLC separations.

Includes:

• TUV Detector (PDA Detector $1000 additional)

• Sample Manager - FTN

• Column Manager to control up to 4 Columns
• Quaternary Solvent Manager
  • 15,000 psi up to 1.0 mL/min,
  • 9000 psi up to 2.0 mL/min
• Computer Loaded with Empower

Specifications:

• Dwell volume (total system): <400 ~L (includes standard 100 ~L mixer)
• Integrated leak management: Leak sensors, as standard, and safe leak handling
• Quantum synchronization: Injection synchronization between pump and sample manager enhances retention time reproducibility
• Set table flow rate range:
  • 01 0 to 2.000 mLlmin, in 0.001 mL increments (firmware version 1.5x and earlier)
  • 010 to 2.200 mL/min, in 0.001 mL increments (firmware version 1.60)
  • 001 to 2.200 mLlmin in 0.001 mL increments (firmware version 1.65 and later)
• Maximum operating pressure:
  • 15,000 psi up to 1.0 mL/min, 9000 psi up to 2.0 mL/min (firmware version l.5x and earlier)
  • 15,000 psi up to 1.0 mL/min, 7800 psi up to 2.2 mL/min (firmware version 1.6x and later)
• pH range: 2 to 12
• Unattended operation: Leak sensors and safe leak handling, full 96-hour diagnostic data display through console software
• Cycle time: <30s Inject to Inject

Optional UPLC Sample Organizer

Optional Service Contract, Installation, and Training also available

The Waters ACQUITY UPLC System provides an integrated configuration for solvent and XYZZ sample management designed for use with ACQUITY UPLC chemistries. The core ACQUITY UPLC System comprises a Binary Solvent Manager, a Sample Manager with integral Column

Heater, and a Solvents Tray. The optional Sample Organizer is available for expanded sample capacity. The full line of Waters MS Technologies products, from single quadrupole to hybrid quadrupole time-of-flight are also available.

Includes:

• TUV Detector or PDA Detector ($1000 more)
• Sample Manager
• Column Heater (1 columns)
  • (Optional Column Manager to control up to 4 Columns $2000 extra)
• Binary Solvent Manager
  • 15000 psi Max Pressure
  • 1034 bars Max Pressure
• Computer Loaded with Empower

Includes 1 year Service Contract, Installation, and a PM at 6 months

Optional TUV or PDA Detector

Developed for integrated UPLC/MS/MS quantitative applications, the Waters ACQUITY TQD features the highest levels of tandem quadrupole MS selectivity, robustness, speed, and accuracy.   To maximize the analytical advantages of UltraPerformance LC (UPLC), the ACQUITY TQD operates at the highest data acquisition rates. When integrated with the ACQUITY UPLC System, the ACQUITY TQD gives you class-leading analytical detection limits, resolution, and sample throughput for all of your quantitative applications: bioanalysis, ADME screening, food safety, environmental monitoring, clinical, forensic, and more.

Includes:

• Waters TQD UPLC/MS/MS Mass Spectrometer
• Enhanced high performance triple quadrupole mass spectrometer system
• Roughing Pump
• Windows 7 Computer loaded w/ Mass Lynx 4.1 SCN 805.IVD
• Flex Cart for UPLC
• Waters Acquity UPLC
• Column Manager
• Sample Manager
• Binary Solvent Manager
• Monitor – Mouse – Keyboard
• Cables and Manuals

Performance Specifications:

• Acquisition modes
  • Full scan MS
  • Product ion scan
  • Precursor ion scan
  • Constant neutral loss
  • Selected Ion Recording (SIR)
  • Multiple Reaction Monitoring (MRM)
• Survey scan modes: Full scan MS triggered product ion scan
• Mass range: 2 to 2048 m/z
• Scan speed: Up to 10,000 Da/s
• Examples of achievable acquisition rates:
  • 10 scans per second (m/z 100 to 1000)
  • 20 scans per second (m/z 50 to 500)
• Mass stability: Mass drift is < 0.1 Da over a 24 hour period
• Linearity of response: The linearity of response relative to sample concentration for a specified compound is five orders of magnitude from the limit of detection
• Polarity switching time: 20 ms to switch between positive and negat ive ion modes
• MRM acquisition rate: Minimum dwell time of 3 ms per MRM channel
• Inter-channel cross talk: The inter-channel cross talk between two MRM transitions, acquired using an MRM dwell time of 10 ms and an inter-channel delay time of 10 ms, will be less than 0.02%.
• Number of MRM channels: Up to 16,384 MRM channels (512 functions, 32 channels per function) can be monitored in a single acquisition; up to 1024 MRM channels when operating in GLP/secure mode (32 functions, 32 channels per function).
• Mass resolution: Automatically adjusted (InteUiStart) to desired resolu tion; The vaUey between the 2034.63 Da and 2035.63 Da peaks is < 12% of the average height of the two peaks
• MRM sensitivity (ESI+) : A 1 pg on-column injection of reserpine will give a chromatographic signal-ta-noise greater than 2,000:1 (Gradient separation, LC mobile phase flow rate of 0.8 mL/min, MRM transition mil 609 > 195).
• MRM sensitivity (ESI-): A five pg loop injection of chloramphenicol, wi th a mobile phase flow rate of 200 ~lImin will give a chromatographic signal-ta-noise for the transition 321 > 152 mlz greater than 180: 1.
• MRM sensitivity (APCI+): A 100 pg loop injection of 17-a-hydroxyprogesterone, with a mobile phase flow rate of 1000 IJLlmin will give a chromatographic signal-ta-noise for the transition 331 > 109 mil > 150:1.

The ACQUITY UPLC TUV Detector is a tunable, dual wavelength ultraviolet/visible (UV/Vis) detector that offers optimal linearity, resolution, and sensitivity for UPLC/UV separations.  It features uniquely designed, patented, light-guiding flow cell technology, low-noise performance (6 μAU), and support for data rates up to 80 Hz.  The ACQUITY UPLC TUV Detector, coupled with our innovative ACQUITY UPLC Column chemistries and separations modules, leverages ultra-low dwell volume and low-noise, high-speed detection to bring productivity, sensitivity, and resolution that are unprecedented in HPLC techniques being used in the laboratory today.

Specifications:

• Wavelength range: 190 to 700 nm
• Wavelength accuracy: ±1 nm
• Linearity range: Deviation at 2.5 AU ≤5%, propylparaben, at 257 nm
• Baseline noise:6 x 10^-3 AU, at 230 nm, 10 mm shunt cell, 1.0 second filter time constant
• Drift: ≤5.0 x 10^-4 AU/hour/°C at 230 nm
• Sampling rate: Up to 80 points/s
• Unattended operation: Leak sensors, full diagnostic data captured through console software
• Light source: Prealigned, intelligent technology Deuterium lamp
• Flow cell design: Light-guiding UPLC flow cell with intelligent chip technology
• Path length: 10 mm (analytical flow cell)
• Cell volume: 500 nL (analytical flow cell)
• Pressure limit: 1000 psi (all flow cells)
• Wetted materials: 316 stainless steel, fused silica, Teflon AF, PEEK (analytical flow cell)
• Optical bandwidth: 5 nm

Includes 1 year Service Contract, Installation, and a PM at 6 months

Method Development and Training also Available

Includes:

• AB Sciex API4000 LC/MS/MS
• Enhanced high performance triple quadrupole mass spectrometer system
• Line transformers
• Turbo V Ion Source (with APCI and ESI probes)
• Roughing Pump
• Windows 7 Computer loaded w/ Analyst Software 1.6
• Cart
• Shimadzu Prominence 20 UFLC  Shimadzu
  • Shimadzu DGU-20A3 Degasser
  • (2)Shimadzu LC_20AD Liquid Chromatograph
  • Shimadzu CTO Column Oven
  • Shimadzu CBM-20A Communication Module
• CTC Autosampler
• Monitor – Mouse – Keyboard
• Cables and Manuals

Specifications:

• Detector type: Pulse Counting CEM
• Dynamic Range: 5 orders of magnitude
• Ionization Sources: ESI, APCI
• Mass Accuracy / Stability: 0.1 Da over 8 hours, 0.15 Da over 24 hours
• Mass Range: 5-3,000
• Polarity Switching Time: 700 ms
• Scan Speed (Quad): 24,000 Da/sec
• Scan Types: Q1 MS, Q3 MS, Product Ion, Precursor Ion, Neutral Loss or Gain, MRM
• Sensitivity MRM Mode: 200 fg Reserpine on column: S/N > 1,200, C.V. < 5%

The Luminex 100 System is a flexible analyzer based on the principles of flow cytometry. The system enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small samples. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid assays, receptor-ligand assays, immunoassays, and enzymatic assays.

Specifications:

• Multiplexing: up to 100 analytes per sample
• Dynamic Range: ≥3.5 logs
• Read Time: 96 well plate in ≤ 45 min (up to 12,800 tests/hour)
• Daily Start up/Shut down: ≤30 min

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack

Specifications:

• Hydraulic system: Two dual piston in series pumps with proprietary servo-controlled variable stroke drive, floating piston design and active inlet valve
• Setable flow range: Setpoints 0.001 - 5 ml/min, in 0.001 ml/min increments
• Flow range 1 - 5.0 ml/min
• Flow precision: < 0.3 % RSD (typically 0.15 %), based on retention time, at 1 ml/min
• Pressure: Operating range 0 400 bar (0 - 5880 psi) up to 5 ml/min
• Pressure pulsation: < 2 %amplitude (typically < 1 %), at 1 ml/min isopropanol, at all pressures > 1 MPa
• Compressibility compensation: User-selectable, based on mobile phase compressibility
• Recommended pH range: 0 - 12.5, solvents with pH < 2.3 should not contain acids which attack stainless steel
• Gradient formation: High-pressure binary mixing, delay volume 180 - 480 μl without mixer, 600 - 900 μl with mixer, dependent on back pressure
• Composition range 1- 99 % or 5 μl/min per channel, whatever is greater
• Composition precision < 0.2 % SD, at 0.1 and 1 ml/min
• Control and data evaluation Agilent ChemStation for LC Control and data evaluation

Can be sold as an individual component or as part of a complete stack

Specifications:

• Injection range: From 0.1 – 100 µl in 0.1 µl increments
• Replicate injections: From 1 – 99 from one vial
• Injection cycle time: Typically 50 s, depending on draw speed and volume
• Sample capacity: 100 × 2 ml vials in tray