Used Lab Equipment

Includes 1 year Service Contract, Installation, and a PM at 6 months

Optional TUV or PDA Detector

Developed for integrated UPLC/MS/MS quantitative applications, the Waters ACQUITY TQD features the highest levels of tandem quadrupole MS selectivity, robustness, speed, and accuracy.   To maximize the analytical advantages of UltraPerformance LC (UPLC), the ACQUITY TQD operates at the highest data acquisition rates. When integrated with the ACQUITY UPLC System, the ACQUITY TQD gives you class-leading analytical detection limits, resolution, and sample throughput for all of your quantitative applications: bioanalysis, ADME screening, food safety, environmental monitoring, clinical, forensic, and more.

Includes:

• Waters TQD UPLC/MS/MS Mass Spectrometer
• Enhanced high performance triple quadrupole mass spectrometer system
• Roughing Pump
• Windows 7 Computer loaded w/ Mass Lynx 4.1 SCN 805.IVD
• Flex Cart for UPLC
• Waters Acquity UPLC
• Column Manager
• Sample Manager
• Binary Solvent Manager
• Monitor – Mouse – Keyboard
• Cables and Manuals

Performance Specifications:

• Acquisition modes
  • Full scan MS
  • Product ion scan
  • Precursor ion scan
  • Constant neutral loss
  • Selected Ion Recording (SIR)
  • Multiple Reaction Monitoring (MRM)
• Survey scan modes: Full scan MS triggered product ion scan
• Mass range: 2 to 2048 m/z
• Scan speed: Up to 10,000 Da/s
• Examples of achievable acquisition rates:
  • 10 scans per second (m/z 100 to 1000)
  • 20 scans per second (m/z 50 to 500)
• Mass stability: Mass drift is < 0.1 Da over a 24 hour period
• Linearity of response: The linearity of response relative to sample concentration for a specified compound is five orders of magnitude from the limit of detection
• Polarity switching time: 20 ms to switch between positive and negat ive ion modes
• MRM acquisition rate: Minimum dwell time of 3 ms per MRM channel
• Inter-channel cross talk: The inter-channel cross talk between two MRM transitions, acquired using an MRM dwell time of 10 ms and an inter-channel delay time of 10 ms, will be less than 0.02%.
• Number of MRM channels: Up to 16,384 MRM channels (512 functions, 32 channels per function) can be monitored in a single acquisition; up to 1024 MRM channels when operating in GLP/secure mode (32 functions, 32 channels per function).
• Mass resolution: Automatically adjusted (InteUiStart) to desired resolu tion; The vaUey between the 2034.63 Da and 2035.63 Da peaks is < 12% of the average height of the two peaks
• MRM sensitivity (ESI+) : A 1 pg on-column injection of reserpine will give a chromatographic signal-ta-noise greater than 2,000:1 (Gradient separation, LC mobile phase flow rate of 0.8 mL/min, MRM transition mil 609 > 195).
• MRM sensitivity (ESI-): A five pg loop injection of chloramphenicol, wi th a mobile phase flow rate of 200 ~lImin will give a chromatographic signal-ta-noise for the transition 321 > 152 mlz greater than 180: 1.
• MRM sensitivity (APCI+): A 100 pg loop injection of 17-a-hydroxyprogesterone, with a mobile phase flow rate of 1000 IJLlmin will give a chromatographic signal-ta-noise for the transition 331 > 109 mil > 150:1.

ACQUITY QDa is as intuitive as an optical detector, with the robustness to handle all of your analyses. Working in harmony with your chromatography, it is pre-optimized to work with your samples, without the sample-specific or user adjustments typical of traditional mass spectrometers. For the first time, any analytical scientist can consistently generate the highest quality mass spectral data, routinely without the need for any special training or expertise thus eliminating any need to contract out and wait for results from specialist analytical service laboratories.

With ACQUITY QDa Detector, you can minimize the risk of unexpected coelutions or components and you can confirm trace components with certainty because of the analytical confidence that mass detection brings. Enhance the analytical value and productivity of each analysis, eliminating the need to run all the additional assays or time-consuming alternative techniques.

Working in synergy with optical detection, you can significantly reduce the chance that a sample component will go undetected.

The ACQUITY QDa Detector is compatible with all Waters ACQUITY UPLC, ACQUITY UPC², Alliance HPLC, and purification LC and SFC systems. The ACQUITY QDa Mass Detector is complementary to your current Waters optical detectors including ACQUITY UPLC PDA, TUV, or FLR, or ACQUITY UPC² PDA optical detectors.

The mass spectral information combines seamlessly into the same workflow, routinely giving you more complete characterization of your separation.

Process, interpret, visualize, and compare the most complex data, and turn it into meaningful information quickly and simply. The ACQUITY QDa Detector is fully compatible with Empower Software, our industry-leading chromatography data software platform.

With integrated optical and mass detector data processing workflows, you can also interrogate mass spectral data in the same way and same workflow as you do PDA data. The ACQUITY QDa Detector is also fully compatible with MassLynx Software and its suite of Application Managers.

Column Heater for Waters Alliance Series

Specifications:

• Temperature Range: 20 to 65 °C in 1 °C increments (5 °C above ambient)

The Waters 2475 Fluorescence Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag. The 2475 FLR Detector sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths, and has the ability to monitor up to four independent wavelength pairs.

Features:

• Innovative axially illuminated flow cell designed for less dispersion, less stray light, less volume, and more pathlength for maximum sensitivity
• Three-dimensional spectral scanning for faster method development and optimization, and enhanced peak identification
• Single or multi-channel operation monitors fluorescence at one or more discrete wavelength pairs
• Low noise performance - Less stray light due to orthogonal optics and the use of mirrors, not lenses, minimize scatter
• Multiple detection modes - 2D, 3D, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence detection methods by quickly determining wavelength maxima
• Integral erbium calibration reference – ensures wavelength accuracy
• Normalized emission units for better bench-to-bench reproducibility

The Waters 2487 Dual λ Absorbance Detector is a two-channel, tunable, ultraviolet/visible (UV/Vis) detector designed for high-performance liquid chromatography (HPLC) applications. The Waters 2487 Dual λ Absorbance Detector can operate either as a stand-alone unit (with a chart recorder or integrator) or as an integral part of a Waters chromatography system.

Specifications:

• Wavelength range: 190 to 700 nm
• Bandwidth: <5 nm
• Wavelength accuracy: +1.0 nm
• Wavelength repeatability: +0.1 nm
• Single λ dry noise: <5 µAU (at 230 nm, 1 –second digital filter, 30-second segments, 10-Hz data rate, analytical cell)
• Wet noise: <8 µAU (at 230 nm, 1-second digital filter 30-second segments, 10-Hz data rate, analytical cell)
• Dual channel noise (dry): <35 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; dry, analytical cell)
• Dual channel noise (wet): <45 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; 1 ml/min, flow of acetonitrile, analytical cell)
• Linearity: <5% at 2.5 AU, propylparaben at 257 nm
• Drift: 1.0 x 10-4 AU/hr. max (∆T = +2°C/hr.)
• Thermal drift: 1.0 x 10-4 AU/°C max (∆T = +2°C/hr.)
• Sensitivity setting range: 0.0001 to 4.0000 AUFS
• Filter setting range
  • Single-wavelength: 0.1 to 5.0 seconds
  • Dual-wavelength: 1 to 50 seconds
• Time-constant: To 5.0-second Hamming filter
• Digital data rates:
  • 1,2,5,10,20,40,80 Hz (single channel)
  • 1,2 Hz (dual channel)
• Analog output data rates (single λ mode):
  • 10,20,40,80 Hz (channel A)
  • 10 Hz only (channel B)
• Lamp source: 30-W High Brightness Deuterium lamp, 0.5 nm aperture, pre-aligned 2000 silicon photodiodes (matched pair)
• Second-order filter: Automatic for wavelengths > 370 nm
• Wavelength calibration filter: Erbium filter, used at startup or on demand
• Flow cell: TaperSlit flow cell design
• Nitrogen purge: Purge fitting present on optics bench
• Path length: 0 mm (standard analytical)
• Cell volume: 10 µL (standard analytical)
• Pressure limit: 6895 kPa (69 bar,1000 psi)
• Materials: 316 stainless steel, fused silica, Tygon
• Operating temperature: 4 to 40 °C (39 to 104 °F)
• Operating humidity: 20% to <95%, noncondensing
  
  

Description - The Waters 2790 features a large, easy-to-use LCD interface. Scientists can take advantage of the quick autostart and optimize their methods with a simple push of a button. Manage microplates with the built in autosampler. This reliable tool is a common work-horse in most analytical and contract labs. Built to meet labs rigorous requirements or routine analysis, this robust instrument is easily found in Pharmaceutical, Food Safety, R&D, Chemistry, Environmental or Quality Control labs. Simplify how you collect data, built-in compliance and validation features. The Empower software is capable of automating method development, archiving methods and customizing data reports.

Optional Detectors:

• 996 PDA Detector:
• 2996  PDA Detector:
• 2998 PDA Detector:
  
• 2487 UV detector:
• 2489 UV Detector:
  
• 2475 FLD detector:
  
• Waters 2410 Refractive Index Detector:
  

The components can be customized to your needs.

Waters 2996 Photo Diode Array (PDA) Detector has uniquely-integrated software and optics innovations that deliver no-compromise chromatographic and spectral sensitivity, reliability and ease of use for analytical and preparative separations. Advanced spectral analysis enhances component recognition and homogeneity assessment.

Features:

• Maximum chromatographic sensitivity means unprecedented trace impurity detection and quantification.
• Optimized spectral sensitivity and Waters purity algorithms mean definitive compound identification and co-elution detection.
• Superior linearity range defined by a fixed optical bandpass
• Simplified optics that are reliable and easy to maintain
• Method development and validation are fast and straight forward with uncomplicated method development tools within Empower
• Compliance-ready for the FDA Electronic Records and Electronic Signatures Rule
• Start-up diagnostics that ensure detector performance prior to use

Specification:

• Linearity range: 5% over -0.1 to 2.0 AU
• Spectral resolution: 1.2 nm per photodiode
• Baseline noise: 5 x 10 -5 AU peak-to-peak
• Drift: 1 x 10 -3 AU/hour at 254 nm (after warmup)
• Operating temperature: 4 to 40 ¡ãC (39 to 104 ¡ãF)
• Operating humidity: 20 to 80% non-condensing
• Wavelength accuracy: 1nm
• Wavelength range: 190 to 800 nm

The Waters 474 Scanning Fluorescence Detector can be used for in-vitro diagnostic testing to analyze many compounds, including diagnostic indicators and therapeutically monitored compounds.

Specifications:

• Wavelength Range: 200 - 700 nm
• Spectrum Band Width Excitation: 18 nm
• Spectrum Band Width Emission: 10, 18 or 40 nm
• Wavelength Accuracy: +/- 2 nm
• Wavelength Repeatability: +/- 0.3 nm
• Flow Cell: 16 uL (standard) or 6 uL
• Maximum Flow Cell Pressure: 16 uL, 10 bar; 5 uL, 34.5 bar
• Dimensions: 18.11 x 11.81 x 5.9 in
• Weight: 41.8 lbs
• Temperature Operation: 10 - 32C
• Temperature Storage: 10 - 60C
• Relative Humidity:  20 - 80%

Specifications:

• Wavelength range: 190-360 nm
• Wavelength accuracy: ± 1 nm
• Wavelength reproducibility: ± 0.1 nm
• Sensitivity range: 0.002 - 2.0 AUFS
• Single wavelength mode
• Deuterium source lamp
• Standard analytical flow cell volume 8 ul
• Manual zero set, mark, time constant
• Signal output 10 mV / 1 V

The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations:

Features:

• Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons
• Non-circular drive gears provide smooth, uniform eluent delivery
• User-programmable flow rate calibration and compressibility compensation factors ensure straightforward pump operation qualification
• Tests to retention time variations of less than 0.1% for highly reproducible peak performance
• Easy-to-read display and keypad for error-free programming
• Automatic purging and flow calibration

Specifications:

• Flow rate precision 0.1%, accuracy 1%
• Flow rate up to10.000 mL/min, in 0.001-mL/min increments
• Maximum operating pressure: 6000 psi

Specifications:

• Dimensions: 5 x 22 inches (29x56 cm)
• Weight: 31 lbs (14.3 kg)
• Wavelength range: 190 to 800 nm
• Wavelength accuracy: +1 nm
• Linearity range: 5% at 2.0 AU, propylparaben, at 258
• Spectral resolution: 2 nm
• Baseline noise: +1.5 x 10-5 AU peak-to-peak, dry, at 254 nm
• Drift: 1 x 10-3 AU/hour at 254 nm (after warm-up)

Specifications:

• Number of solvents: Up to four: in any combination of two: Al or A2 and Bl or B2
• Solvent storage: Solvents Tray accommodates up to four chromatographic solvents, two Sample Manager wash solvents and one Binary Solvent Manager seal wash solvent
• Solvent conditioning: Vacuum degassing (Six-channel): one channel per solvent, and two channels for Sample Manager wash solvents
• Operating flow rate range: 010 to 2.000 mL/min, in 0.001 mL increments
• Compressibility compensation: Automatic and continuous
• Effective system delay volume: <120 ulL, independent of system backpressure (with standard 50-ilL mixer installed)
• Plunger seal wash: Integral, active, programmable
• Gradient profiles: Eleven gradient curves [including linear, step (2), concave (4), and convex (4)]
• Wet prime: Automatic
• Maximum operating pressure: 15,000 psi up to 1 mL/min, 9,000 psi up to 2 mL/min per pump, not more than 15,000 psi total
• Composition accuracy:
  • ±0.5% absolute (full scale) from 5 to 95% of flow rates
  • from 0.5 to 2.0 mLlmin (Contact Waters for conditions used)
• Composition precision: 0.15% RSO or ±0.04 min SO, whichever is greater, based on retention time
• Flow precision: 0.075% RSO or ±0.02 min SO, six replicates, based on retention time or volumetric measures (0.500 to 2.000 ml/min)
• Flow accuracy: ± 1.0% at 0.5 mL/min with degassed methanol, per Waters AQT/SystemsQT protocol
• Primary wetted materials: 316 stainless steel, UHMWPE, sapphire, ruby, FEP, PTFE, ETFE, diamond-like coating, PEEK and PEEK alloys, titanium alloys
• Unattended operation: Leak sensors, full diagnostic data captured through console software

Specifications:

• Column capacity:
  • CM-A: Two columns, as standard (maximum length of 150 mm with filter or guard column)
  • or four columns (maximum length of 50 mm) can be supported with optional tubing kit,
  • up to 4.6 mm internal diameter (1.0.)
  • CM-Aux: Two columns (maximum length of 150 mm, with filter or guard column) - up to
  • two CM-Aux units can be configured with one CM-A for support of up to six columns
• Switching valves: Two nine-port, eight-position valves (CM-A only); provides programmable, automatic, random access switching, waste and bypass positions for rapid solvent changeover
• Temperature Range:
  • 0 to 90.0 ·C, set table in 0.1 ·C increments
  • Two independent heatl cool zones per module, up to six zones in stacked configuration
• Temperature Accuracy: ±0.5 ·C
• Temperature Stability: ±0.3 ·C
• Solvent conditioning: Active pre-heating as standard
• Column tracking: eCord Technology column information management tracks and archives column usage history
• 20 support: Optional

The Waters ACQUITY UPLC H-Class System delivers the flexibility of quaternary solvent blending with the advanced performance of UPLC® separations. The system's holistic design is targeted for routine analysis and method development use and is perfectly suited for running both HPLC and UPLC applications while still realizing the improved resolution and sensitivity of UPLC separations.

Includes:

• TUV Detector (PDA Detector $1000 additional)

• Sample Manager - FTN

• Column Manager to control up to 4 Columns
• Quaternary Solvent Manager
  • 15,000 psi up to 1.0 mL/min,
  • 9000 psi up to 2.0 mL/min
• Computer Loaded with Empower

Specifications:

• Dwell volume (total system): <400 ~L (includes standard 100 ~L mixer)
• Integrated leak management: Leak sensors, as standard, and safe leak handling
• Quantum synchronization: Injection synchronization between pump and sample manager enhances retention time reproducibility
• Set table flow rate range:
  • 01 0 to 2.000 mLlmin, in 0.001 mL increments (firmware version 1.5x and earlier)
  • 010 to 2.200 mL/min, in 0.001 mL increments (firmware version 1.60)
  • 001 to 2.200 mLlmin in 0.001 mL increments (firmware version 1.65 and later)
• Maximum operating pressure:
  • 15,000 psi up to 1.0 mL/min, 9000 psi up to 2.0 mL/min (firmware version l.5x and earlier)
  • 15,000 psi up to 1.0 mL/min, 7800 psi up to 2.2 mL/min (firmware version 1.6x and later)
• pH range: 2 to 12
• Unattended operation: Leak sensors and safe leak handling, full 96-hour diagnostic data display through console software
• Cycle time: <30s Inject to Inject

Specifications:

• Number of sample plates: Total of two plates, expandable to up to 22 plates with optional Sample Organizer (see below):
  • 96 and 384 microtiter plates
  • 48 position 2.00-mL vial plates
  • 48 position 0.65-mL micro-centrifuge tube plates
  • 24 position 1.50-mL micro-centrifuge tube plates
• Maximum sample capacity: 768 in two 384-well plates; expandable to up to 8,448 samples with optional Sample Organizer
• Number of sample injections: 1 to 99 injections per sample
• Injection volume range: 0.1 to 50.0 iJL, in 0.1 iJL increments, partial or full loop mode; 10ul loop is standard; 1, 2, 5, 20, and 50-uL loops also available
• Sample delivery precision(full loop injection mode): < 0.3% RSO, full loop, standard lO-iJL loop (default wash/purge conditions), per Waters AQT/SystemsQT protocol
• Sample delivery precision(PLNO injection mode): < 1% RSO within 20% to 75% of loop volume for 1, 2, 5, 10, 20, and 50-iJL loops, UV detection
• Injector linearity: > 0.999 coefficient of deviation (from 20% to 75%, partial loop overfill mode (PLNO injection mode), per Waters' AQT/SystemsQT protocol
• Sample temperature control:0 to 40.0 ·C, settable in 0.1 ·C increments (assumes an ambient temperature of 25.0 ·C). At an ambient temperature of 21.0 ·C or lower the sample manager will maintain the temperature of the sample compartment down to 4.0 ·C with a tolerance of -2.0/+6.0 ·C, when configured with the maximum number of vials and/or plates
• Injection cycle time:
  • < 15 sec between multipe injections with "load ahead" enabled
  • 30 sec with single weak wash, lO-iJL loop, pressure assist mode
• Sample probe: XYZZ based needle-in-needle design
• Minimum sample required: 5 uL residual, using maximum recovery 2-mL vials (zero offset)
• Wash solvents: Two degassed: strong solvent and weak wash solvent, programmable to suit application
• Sample carryover: < 0.005% or < 2.000 nL, whichever is greater
• Advanced operations: Loop off-line mode, load ahead
• Unattended operation: Leak sensors, full diagnostic data control captured through console software
• Primary wetted material: Titanium alloy, 316 stainless steel, fluoropolymer, fluoroelastomer, PPS alloy, PEEK alloy, PPS, PEEK, OLC coating, gold