Used Lab Equipment

Includes 1 year Service Contract, Installation, and a PM at 6 months

Optional TUV or PDA Detector

Developed for integrated UPLC/MS/MS quantitative applications, the Waters ACQUITY TQD features the highest levels of tandem quadrupole MS selectivity, robustness, speed, and accuracy.   To maximize the analytical advantages of UltraPerformance LC (UPLC), the ACQUITY TQD operates at the highest data acquisition rates. When integrated with the ACQUITY UPLC System, the ACQUITY TQD gives you class-leading analytical detection limits, resolution, and sample throughput for all of your quantitative applications: bioanalysis, ADME screening, food safety, environmental monitoring, clinical, forensic, and more.

Includes:

• Waters TQD UPLC/MS/MS Mass Spectrometer
• Enhanced high performance triple quadrupole mass spectrometer system
• Roughing Pump
• Windows 7 Computer loaded w/ Mass Lynx 4.1 SCN 805.IVD
• Flex Cart for UPLC
• Waters Acquity UPLC
• Column Manager
• Sample Manager
• Binary Solvent Manager
• Monitor – Mouse – Keyboard
• Cables and Manuals

Performance Specifications:

• Acquisition modes
  • Full scan MS
  • Product ion scan
  • Precursor ion scan
  • Constant neutral loss
  • Selected Ion Recording (SIR)
  • Multiple Reaction Monitoring (MRM)
• Survey scan modes: Full scan MS triggered product ion scan
• Mass range: 2 to 2048 m/z
• Scan speed: Up to 10,000 Da/s
• Examples of achievable acquisition rates:
  • 10 scans per second (m/z 100 to 1000)
  • 20 scans per second (m/z 50 to 500)
• Mass stability: Mass drift is < 0.1 Da over a 24 hour period
• Linearity of response: The linearity of response relative to sample concentration for a specified compound is five orders of magnitude from the limit of detection
• Polarity switching time: 20 ms to switch between positive and negat ive ion modes
• MRM acquisition rate: Minimum dwell time of 3 ms per MRM channel
• Inter-channel cross talk: The inter-channel cross talk between two MRM transitions, acquired using an MRM dwell time of 10 ms and an inter-channel delay time of 10 ms, will be less than 0.02%.
• Number of MRM channels: Up to 16,384 MRM channels (512 functions, 32 channels per function) can be monitored in a single acquisition; up to 1024 MRM channels when operating in GLP/secure mode (32 functions, 32 channels per function).
• Mass resolution: Automatically adjusted (InteUiStart) to desired resolu tion; The vaUey between the 2034.63 Da and 2035.63 Da peaks is < 12% of the average height of the two peaks
• MRM sensitivity (ESI+) : A 1 pg on-column injection of reserpine will give a chromatographic signal-ta-noise greater than 2,000:1 (Gradient separation, LC mobile phase flow rate of 0.8 mL/min, MRM transition mil 609 > 195).
• MRM sensitivity (ESI-): A five pg loop injection of chloramphenicol, wi th a mobile phase flow rate of 200 ~lImin will give a chromatographic signal-ta-noise for the transition 321 > 152 mlz greater than 180: 1.
• MRM sensitivity (APCI+): A 100 pg loop injection of 17-a-hydroxyprogesterone, with a mobile phase flow rate of 1000 IJLlmin will give a chromatographic signal-ta-noise for the transition 331 > 109 mil > 150:1.

Column Heater for Waters Alliance Series

Specifications:

• Temperature Range: 20 to 65 °C in 1 °C increments (5 °C above ambient)

The 2424 Evaporative Light Scattering (ELS) Detector is a compact detector that controls temperatures in both the nebulization and evaporation stages, maintaining low-dispersion characteristics for dependable HPLC/ELS results. It allows you to obtain more peak information per run and reliability and reproducibility for LC and LC/MS.

Use the detector as part of an Alliance® HPLC or LC/MS system under the direct control of either Empower or MassLynx Software, or as a stand-alone ELS module. This open access-ready detector integrates with the AutoPurification System for flexible, multi-detector purification and isolation solutions.

The 2424 ELS Detector provides solutions for the analysis and purification of a wide variety of compounds that lack UV/Vis chromophores including triglycerides, sugars and natural products.

Specifications:

• Nebulizer: 300 to 3000 µL/min, 1.80 standard liters per minute (Ls/min) at 170 kPa (1.7 bar, 25 psi)
• -High-flow rate (standard with detector)
• Low-flow rate: 50 to 500 µl/min, 0.77 Ls/min at 170 kPa (1.7 bar, 25 psi)
• Gas: 140 kPa (1.4 bar, 20 psi) to 410 kPa (4.1 bar, 60 psi)
• Gain settings : 0 to 1000
• Time constant filter settings: to 5.0 seconds (Hamming)
• Two analog outputs :
• Channel 1 = -0.1 to 2.1 VDC (selectable maximum rates are 10,20,40, or 80 Hz)
• Channel 2 = -0.1 to 2.1 VDC (fixed maximum data rate is 10 Hz)
• Four event input
• Input voltage: +30 Volts max
• Low input voltage: <1.7V
• High input voltage:> 3.2 V
• Two event outputs
• Type: Contact closure
• Voltage:+30 Volts max
• Current, switching: 0.5 A
• Current, carry: 1.2 A
• Temperature control:
• Nebulizer heater power: 0 to 100%
• Nebulizer cooler: Cooling/Off
• Drift tube heater: Ambient to 100°C (212 °F)
• Operating temperature: 4 to 30°C (39.2 to 86°F)
• Operating humidity: 20 to 95%, noncondensing
• Shipping and storage temperature: -30 to 60°C (-22 to 140°F)
• Shipping and storage humidity: 0 to 95%, noncondensing
• Acoustic noise (instrument generated): <65 dBA

The Waters 2475 Fluorescence Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag. The 2475 FLR Detector sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths, and has the ability to monitor up to four independent wavelength pairs.

Features:

• Innovative axially illuminated flow cell designed for less dispersion, less stray light, less volume, and more pathlength for maximum sensitivity
• Three-dimensional spectral scanning for faster method development and optimization, and enhanced peak identification
• Single or multi-channel operation monitors fluorescence at one or more discrete wavelength pairs
• Low noise performance - Less stray light due to orthogonal optics and the use of mirrors, not lenses, minimize scatter
• Multiple detection modes - 2D, 3D, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence detection methods by quickly determining wavelength maxima
• Integral erbium calibration reference – ensures wavelength accuracy
• Normalized emission units for better bench-to-bench reproducibility

The Waters 2487 Dual λ Absorbance Detector is a two-channel, tunable, ultraviolet/visible (UV/Vis) detector designed for high-performance liquid chromatography (HPLC) applications. The Waters 2487 Dual λ Absorbance Detector can operate either as a stand-alone unit (with a chart recorder or integrator) or as an integral part of a Waters chromatography system.

Specifications:

• Wavelength range: 190 to 700 nm
• Bandwidth: <5 nm
• Wavelength accuracy: +1.0 nm
• Wavelength repeatability: +0.1 nm
• Single λ dry noise: <5 µAU (at 230 nm, 1 –second digital filter, 30-second segments, 10-Hz data rate, analytical cell)
• Wet noise: <8 µAU (at 230 nm, 1-second digital filter 30-second segments, 10-Hz data rate, analytical cell)
• Dual channel noise (dry): <35 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; dry, analytical cell)
• Dual channel noise (wet): <45 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; 1 ml/min, flow of acetonitrile, analytical cell)
• Linearity: <5% at 2.5 AU, propylparaben at 257 nm
• Drift: 1.0 x 10-4 AU/hr. max (∆T = +2°C/hr.)
• Thermal drift: 1.0 x 10-4 AU/°C max (∆T = +2°C/hr.)
• Sensitivity setting range: 0.0001 to 4.0000 AUFS
• Filter setting range
  • Single-wavelength: 0.1 to 5.0 seconds
  • Dual-wavelength: 1 to 50 seconds
• Time-constant: To 5.0-second Hamming filter
• Digital data rates:
  • 1,2,5,10,20,40,80 Hz (single channel)
  • 1,2 Hz (dual channel)
• Analog output data rates (single λ mode):
  • 10,20,40,80 Hz (channel A)
  • 10 Hz only (channel B)
• Lamp source: 30-W High Brightness Deuterium lamp, 0.5 nm aperture, pre-aligned 2000 silicon photodiodes (matched pair)
• Second-order filter: Automatic for wavelengths > 370 nm
• Wavelength calibration filter: Erbium filter, used at startup or on demand
• Flow cell: TaperSlit flow cell design
• Nitrogen purge: Purge fitting present on optics bench
• Path length: 0 mm (standard analytical)
• Cell volume: 10 µL (standard analytical)
• Pressure limit: 6895 kPa (69 bar,1000 psi)
• Materials: 316 stainless steel, fused silica, Tygon
• Operating temperature: 4 to 40 °C (39 to 104 °F)
• Operating humidity: 20% to <95%, noncondensing
  
  

The 2707 Autosampler is a versatile, compact sample management system that makes highly precise and reproducible injections of samples into your LC or LC/MS system. It is ideally suited for laboratories where reduced carryover, maximized repeatability, high accuracy, and application flexibility are of concern.

Features:

• Compact design yet easily serviceable
• High-resolution syringe control for high-precision injections
• Interchangeable fixed-volume sample loops
• Variable-volume partial-loop injection capability
• Optional sample cooling for consistent results
• Pressure-assisted sample aspiration injection capability
• Use of plates or vials, alone or in combination, for varied sampling formats
• Empower Software control

Specifications:

• Audible noise level: <65 dBA
• Working temperature: 10 to 40 °C (indoor use only)
• Storage temperature: -25 to +60 °C
• Humidity: 20% to 80% RH
• Installation class: II
• Pollution degree: 2
• Altitude: Up to 2000 m
• Power requirements: 95 to 240 VAC ±10%; 50 to 60 Hz; 200VA
• Viscosity range: 1 to 5.0 cP
• Back pressure (maximum): 5000 psi (345 bar)
• Sample capacity: 2 microtiter plates according to SBS standards; 96-well high/low and 384-well low formats, 48-vial or 12-vial trays; any combination of plates is allowed
• Vial/plate dimensions (includes cap): Maximum vial/plate height: 4.7 cm (1.9 in.) (includes septa or cap mat)
• Loop volumes: 5, 10, 20, 100 (standard), and 500 μL; 10 mL loop (standard) for prep option
• Dispenser syringe: 500 μL standard or 2500 μL μL (standard for prep option)
• Vial detection: Missing vial/well plate detection by sensor
• Headspace pressure: Built-in compressor, only used for vials with septa
• Switching time injection valve: Electrically <100 msec
• Wash solvent: Integrated wash solvent bottle
• Wetted parts in flow path: SS316, PTFE, TEFZEL, PEEK, Glass
• Injection cycle time: <60 s for one injection, ≤100 μL including 300 μL wash

Description - The Waters 2790 features a large, easy-to-use LCD interface. Scientists can take advantage of the quick autostart and optimize their methods with a simple push of a button. Manage microplates with the built in autosampler. This reliable tool is a common work-horse in most analytical and contract labs. Built to meet labs rigorous requirements or routine analysis, this robust instrument is easily found in Pharmaceutical, Food Safety, R&D, Chemistry, Environmental or Quality Control labs. Simplify how you collect data, built-in compliance and validation features. The Empower software is capable of automating method development, archiving methods and customizing data reports.

Optional Detectors:

• 996 PDA Detector:
• 2996  PDA Detector:
• 2998 PDA Detector:
  
• 2487 UV detector:
• 2489 UV Detector:
  
• 2475 FLD detector:
  
• Waters 2410 Refractive Index Detector:
  

The components can be customized to your needs.

Waters 2996 Photo Diode Array (PDA) Detector has uniquely-integrated software and optics innovations that deliver no-compromise chromatographic and spectral sensitivity, reliability and ease of use for analytical and preparative separations. Advanced spectral analysis enhances component recognition and homogeneity assessment.

Features:

• Maximum chromatographic sensitivity means unprecedented trace impurity detection and quantification.
• Optimized spectral sensitivity and Waters purity algorithms mean definitive compound identification and co-elution detection.
• Superior linearity range defined by a fixed optical bandpass
• Simplified optics that are reliable and easy to maintain
• Method development and validation are fast and straight forward with uncomplicated method development tools within Empower
• Compliance-ready for the FDA Electronic Records and Electronic Signatures Rule
• Start-up diagnostics that ensure detector performance prior to use

Specification:

• Linearity range: 5% over -0.1 to 2.0 AU
• Spectral resolution: 1.2 nm per photodiode
• Baseline noise: 5 x 10 -5 AU peak-to-peak
• Drift: 1 x 10 -3 AU/hour at 254 nm (after warmup)
• Operating temperature: 4 to 40 ¡ãC (39 to 104 ¡ãF)
• Operating humidity: 20 to 80% non-condensing
• Wavelength accuracy: 1nm
• Wavelength range: 190 to 800 nm

Waters Autopurification System Includes:

• 3100 Mass Detector
• 1525 Binary HPLC Pump (0-22.5 mL/min)
• System Fluidics Organizer (SFO)
• 515 Make-up Pump
• 2489 UV/Vis Detector
• 2767 Sample Manager
• Sogevac SV40 BI Vacuum Pump
• PC with MassLynx 4.1

The Waters 474 Scanning Fluorescence Detector can be used for in-vitro diagnostic testing to analyze many compounds, including diagnostic indicators and therapeutically monitored compounds.

Specifications:

• Wavelength Range: 200 - 700 nm
• Spectrum Band Width Excitation: 18 nm
• Spectrum Band Width Emission: 10, 18 or 40 nm
• Wavelength Accuracy: +/- 2 nm
• Wavelength Repeatability: +/- 0.3 nm
• Flow Cell: 16 uL (standard) or 6 uL
• Maximum Flow Cell Pressure: 16 uL, 10 bar; 5 uL, 34.5 bar
• Dimensions: 18.11 x 11.81 x 5.9 in
• Weight: 41.8 lbs
• Temperature Operation: 10 - 32C
• Temperature Storage: 10 - 60C
• Relative Humidity:  20 - 80%

Specifications:

• Wavelength range: 190-360 nm
• Wavelength accuracy: ± 1 nm
• Wavelength reproducibility: ± 0.1 nm
• Sensitivity range: 0.002 - 2.0 AUFS
• Single wavelength mode
• Deuterium source lamp
• Standard analytical flow cell volume 8 ul
• Manual zero set, mark, time constant
• Signal output 10 mV / 1 V

The 515 HPLC pump is a programmable, free-standing HPLC pump available in isocratic, gradient, and semi-preparative configurations:

Features:

• Precise stepper motor control (48 steps/µL resolution) of dual reciprocating pistons
• Non-circular drive gears provide smooth, uniform eluent delivery
• User-programmable flow rate calibration and compressibility compensation factors ensure straightforward pump operation qualification
• Tests to retention time variations of less than 0.1% for highly reproducible peak performance
• Easy-to-read display and keypad for error-free programming
• Automatic purging and flow calibration

Specifications:

• Flow rate precision 0.1%, accuracy 1%
• Flow rate up to10.000 mL/min, in 0.001-mL/min increments
• Maximum operating pressure: 6000 psi

The Waters 717plus Autosampler injects samples into a high performance liquid chromatography (HPLC) system. The Waters 717plus automates sampling by storing up to 96 sampling methods or “steps”. Each method controls all injection parameters for a sequence of vials.

The Waters 717plus may be used as either a stand-alone unit or as a component in a Waters IEEE-488 based HPLC system. As part of an IEEE-488 based system, the autosampler may be controlled by Waters PowerLine System Controllers or Waters Data Workstations.

Specifications:

• Dimensions: 5 x 22 inches (29x56 cm)
• Weight: 31 lbs (14.3 kg)
• Wavelength range: 190 to 800 nm
• Wavelength accuracy: +1 nm
• Linearity range: 5% at 2.0 AU, propylparaben, at 258
• Spectral resolution: 2 nm
• Baseline noise: +1.5 x 10-5 AU peak-to-peak, dry, at 254 nm
• Drift: 1 x 10-3 AU/hour at 254 nm (after warm-up)