Used Lab Equipment

Waters Acquity/TQD system from 2012 including:

• ACQUITY I-Class BSM, binary solvent manager
• ACQUITY I-Class SM-FL, sample manager
• ACQUITY H-Class Col Mgr-Active, column manager with 2 column positions
• ACQUITY PDA DETECTOR, uv detector
• XEVO TQD, mass spec detector
• Computer loaded with Mass Lynx/Empower software
• Roughing pump and UPS backup

Physical Specifications

• Height: 57.1 cm (22.5 inches)
• Depth: 57.1 cm (22.5 inches); 64.8 cm (25.5 inches) with optional sample heater/cooler
• Width: 45.7 cm (18 inches); 58.4 cm (23.0 inches) with optional column heater or column cooler
• Weight: 48 kg (107 lb), or 62.1 kg (137 lb) with optional sample heater/cooler and column heater/cooler module.
• Wetted surface materials: 316 stainless steel, ruby, sapphire, MP35N, PEEK, PPS, UHMWPE, Tefzel (ETFE), Teflon (FEP and PTFE), Teflon AF, Fluorology G, Fluorogy-08R

Environmental Specifications

• Operating temperature: 4 to 40°C (39 to 104°F)
• Relative humidity: 20 to 80%, non-condensing
• Acoustic noise: <65 dB(A)
• Solvent compatibility: Solvent Consistent with materials of construction. Salts and buffers can reduce seal life, especially at pressure in excess of 3000 psi.

Electrical Specifications

• Power requirements: 950 VA (maximum)
• Line voltage: 100 - 240 Vac
• Frequency: 50 - 60 Hz

Waters 2996 Photo Diode Array (PDA) Detector has uniquely-integrated software and optics innovations that deliver no-compromise chromatographic and spectral sensitivity, reliability and ease of use for analytical and preparative separations. Advanced spectral analysis enhances component recognition and homogeneity assessment.

Features:

• Maximum chromatographic sensitivity means unprecedented trace impurity detection and quantification.
• Optimized spectral sensitivity and Waters purity algorithms mean definitive compound identification and co-elution detection.
• Superior linearity range defined by a fixed optical bandpass
• Simplified optics that are reliable and easy to maintain
• Method development and validation are fast and straight forward with uncomplicated method development tools within Empower
• Compliance-ready for the FDA Electronic Records and Electronic Signatures Rule
• Start-up diagnostics that ensure detector performance prior to use

Specification:

• Linearity range: 5% over -0.1 to 2.0 AU
• Spectral resolution: 1.2 nm per photodiode
• Baseline noise: 5 x 10 -5 AU peak-to-peak
• Drift: 1 x 10 -3 AU/hour at 254 nm (after warmup)
• Operating temperature: 4 to 40 ¡ãC (39 to 104 ¡ãF)
• Operating humidity: 20 to 80% non-condensing
• Wavelength accuracy: 1nm
• Wavelength range: 190 to 800 nm

This can be purchase with a number of optional detectors.
Description - The Waters 2695 features a large, easy-to-use LCD interface. Scientists can take advantage of the quick autostart and optimize their methods with a simple push of a button. Manage up to 120 industry standard vials with the built in autosampler. This reliable tool is a common work-horse in most analytical and contract labs. Built to meet labs rigorous requirements or routine analysis, this robust instrument is easily found in Pharmaceutical, Food Safety, R&D, Chemistry, Environmental or Quality Control labs. Simplify how you collect data, built-in compliance and validation features. The Empower software is capable of automating method development, archiving methods and customizing data reports.

The components can be customized to your needs.

You can get the system with any of the detectors, as well as an optional Computer.

• 996 PDA Detector
• 2996  PDA Detector
• 2998 PDA Detector
• 2487 UV detector
• 2489 UV Detector
• 2475 FLD detector:
• Waters 2410 Refractive Index Detector:
  
• Computer Loaded with Empowers 2 Software

This can be purchase with a number of optional detectors.
Description - The Waters 2695 features a large, easy-to-use LCD interface. Scientists can take advantage of the quick autostart and optimize their methods with a simple push of a button. Manage up to 120 industry standard vials with the built in autosampler. This reliable tool is a common work-horse in most analytical and contract labs. Built to meet labs rigorous requirements or routine analysis, this robust instrument is easily found in Pharmaceutical, Food Safety, R&D, Chemistry, Environmental or Quality Control labs. Simplify how you collect data, built-in compliance and validation features. The Empower software is capable of automating method development, archiving methods and customizing data reports.

The components can be customized to your needs.

You can get the system with any of the detectors, as well as an optional Computer.

• 996 PDA Detector
• 2996  PDA Detector
• 2998 PDA Detector
• 2487 UV detector
• 2489 UV Detector
• 2475 FLD detector:
• Waters 2410 Refractive Index Detector:
  
• Computer Loaded with Empowers 2 Software

Transferring sample aliquots from dissolution bath vessels to dissolution dispenser, this high throughput, automated, HPLC platform provides fast and accurate results to suit all HPLC needs.

Specifications:

• Capacity: 5 x 24-Position Carousels
• Number of Syringes: 6 to 8 (depending on configuration)
• Accessories: Power On/Off LED on front panel

Column Heater for Waters Alliance Series

Specifications:

• Temperature Range: 20 to 65 °C in 1 °C increments (5 °C above ambient)

Specifications:

• Dimensions: 5 x 22 inches (29x56 cm)
• Weight: 31 lbs (14.3 kg)
• Wavelength range: 190 to 800 nm
• Wavelength accuracy: +1 nm
• Linearity range: 5% at 2.0 AU, propylparaben, at 258
• Spectral resolution: 2 nm
• Baseline noise: +1.5 x 10-5 AU peak-to-peak, dry, at 254 nm
• Drift: 1 x 10-3 AU/hour at 254 nm (after warm-up)

The Waters 2475 Fluorescence Detector offers unsurpassed sensitivity for quantifying low concentrations of target compounds, whether naturally fluorescent or derivatized with a fluorescent tag. The 2475 FLR Detector sets the standard for fluorescence method development with 3D and “on-the-fly” spectral scanning for determining the optimal emission and excitation wavelengths, and has the ability to monitor up to four independent wavelength pairs.

Features:

• Innovative axially illuminated flow cell designed for less dispersion, less stray light, less volume, and more pathlength for maximum sensitivity
• Three-dimensional spectral scanning for faster method development and optimization, and enhanced peak identification
• Single or multi-channel operation monitors fluorescence at one or more discrete wavelength pairs
• Low noise performance - Less stray light due to orthogonal optics and the use of mirrors, not lenses, minimize scatter
• Multiple detection modes - 2D, 3D, and on-the-fly spectral scanning – can greatly reduce the time needed to develop fluorescence detection methods by quickly determining wavelength maxima
• Integral erbium calibration reference – ensures wavelength accuracy
• Normalized emission units for better bench-to-bench reproducibility

The Waters 474 Scanning Fluorescence Detector can be used for in-vitro diagnostic testing to analyze many compounds, including diagnostic indicators and therapeutically monitored compounds.

Specifications:

• Wavelength Range: 200 - 700 nm
• Spectrum Band Width Excitation: 18 nm
• Spectrum Band Width Emission: 10, 18 or 40 nm
• Wavelength Accuracy: +/- 2 nm
• Wavelength Repeatability: +/- 0.3 nm
• Flow Cell: 16 uL (standard) or 6 uL
• Maximum Flow Cell Pressure: 16 uL, 10 bar; 5 uL, 34.5 bar
• Dimensions: 18.11 x 11.81 x 5.9 in
• Weight: 41.8 lbs
• Temperature Operation: 10 - 32C
• Temperature Storage: 10 - 60C
• Relative Humidity:  20 - 80%

The 2424 Evaporative Light Scattering (ELS) Detector is a compact detector that controls temperatures in both the nebulization and evaporation stages, maintaining low-dispersion characteristics for dependable HPLC/ELS results. It allows you to obtain more peak information per run and reliability and reproducibility for LC and LC/MS.

Use the detector as part of an Alliance® HPLC or LC/MS system under the direct control of either Empower or MassLynx Software, or as a stand-alone ELS module. This open access-ready detector integrates with the AutoPurification System for flexible, multi-detector purification and isolation solutions.

The 2424 ELS Detector provides solutions for the analysis and purification of a wide variety of compounds that lack UV/Vis chromophores including triglycerides, sugars and natural products.

Specifications:

• Nebulizer: 300 to 3000 µL/min, 1.80 standard liters per minute (Ls/min) at 170 kPa (1.7 bar, 25 psi)
• -High-flow rate (standard with detector)
• Low-flow rate: 50 to 500 µl/min, 0.77 Ls/min at 170 kPa (1.7 bar, 25 psi)
• Gas: 140 kPa (1.4 bar, 20 psi) to 410 kPa (4.1 bar, 60 psi)
• Gain settings : 0 to 1000
• Time constant filter settings: to 5.0 seconds (Hamming)
• Two analog outputs :
• Channel 1 = -0.1 to 2.1 VDC (selectable maximum rates are 10,20,40, or 80 Hz)
• Channel 2 = -0.1 to 2.1 VDC (fixed maximum data rate is 10 Hz)
• Four event input
• Input voltage: +30 Volts max
• Low input voltage: <1.7V
• High input voltage:> 3.2 V
• Two event outputs
• Type: Contact closure
• Voltage:+30 Volts max
• Current, switching: 0.5 A
• Current, carry: 1.2 A
• Temperature control:
• Nebulizer heater power: 0 to 100%
• Nebulizer cooler: Cooling/Off
• Drift tube heater: Ambient to 100°C (212 °F)
• Operating temperature: 4 to 30°C (39.2 to 86°F)
• Operating humidity: 20 to 95%, noncondensing
• Shipping and storage temperature: -30 to 60°C (-22 to 140°F)
• Shipping and storage humidity: 0 to 95%, noncondensing
• Acoustic noise (instrument generated): <65 dBA

Includes:

• Waters ZQ Single Quadrupole Detector
• Computer w/ MassLynx software
• Monitor and Cables
• Manuals

The Micromass-ZQ 2000 (Mass Selective Detector) is a robust single quadrupole mass spectrometer, offering characteristic performance for laboratories that require nominal mass resolution for mass confirmation applications.  This system has a mass range (amu) up to 2000 daltons.  Easily qualify and quantify small molecules in a flash with the highly sensitive system.  Both inlet probes are quickly and easily interchangeable without breaking vacuum.  Flow rates allowed are 5-1000 µl/min (ESI) and 200-2000 µl/min (APCI).

Specifications:

• Air Cooled System
• Mass Range 2000amu
• ZSpray dual orthogonal sampling interface provides unsurpassed sensitivity.
• Vertically mounted liquid inlet probes to minimize tube length and peak dispersion
• Software-controlled, intergrated syringe pump for easy calibration, tuning and sample infusion
• Digital control of source gas flow rates
• Software controlled divert valve/manual injection valve
• Differentially pumped vacuum system featuring singular air-cooled, split flow turbomolecular pump technology
• Sensiticity: 1 pg Reserpine
• Mass Range: 2 – 2000

The Waters 2487 Dual λ Absorbance Detector is a two-channel, tunable, ultraviolet/visible (UV/Vis) detector designed for high-performance liquid chromatography (HPLC) applications. The Waters 2487 Dual λ Absorbance Detector can operate either as a stand-alone unit (with a chart recorder or integrator) or as an integral part of a Waters chromatography system.

Specifications:

• Wavelength range: 190 to 700 nm
• Bandwidth: <5 nm
• Wavelength accuracy: +1.0 nm
• Wavelength repeatability: +0.1 nm
• Single λ dry noise: <5 µAU (at 230 nm, 1 –second digital filter, 30-second segments, 10-Hz data rate, analytical cell)
• Wet noise: <8 µAU (at 230 nm, 1-second digital filter 30-second segments, 10-Hz data rate, analytical cell)
• Dual channel noise (dry): <35 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; dry, analytical cell)
• Dual channel noise (wet): <45 µAU (measured at 230 nm, running at 230 to 280 nm with 2-second filter at 1-Hz data rate; 1 ml/min, flow of acetonitrile, analytical cell)
• Linearity: <5% at 2.5 AU, propylparaben at 257 nm
• Drift: 1.0 x 10-4 AU/hr. max (∆T = +2°C/hr.)
• Thermal drift: 1.0 x 10-4 AU/°C max (∆T = +2°C/hr.)
• Sensitivity setting range: 0.0001 to 4.0000 AUFS
• Filter setting range
  • Single-wavelength: 0.1 to 5.0 seconds
  • Dual-wavelength: 1 to 50 seconds
• Time-constant: To 5.0-second Hamming filter
• Digital data rates:
  • 1,2,5,10,20,40,80 Hz (single channel)
  • 1,2 Hz (dual channel)
• Analog output data rates (single λ mode):
  • 10,20,40,80 Hz (channel A)
  • 10 Hz only (channel B)
• Lamp source: 30-W High Brightness Deuterium lamp, 0.5 nm aperture, pre-aligned 2000 silicon photodiodes (matched pair)
• Second-order filter: Automatic for wavelengths > 370 nm
• Wavelength calibration filter: Erbium filter, used at startup or on demand
• Flow cell: TaperSlit flow cell design
• Nitrogen purge: Purge fitting present on optics bench
• Path length: 0 mm (standard analytical)
• Cell volume: 10 µL (standard analytical)
• Pressure limit: 6895 kPa (69 bar,1000 psi)
• Materials: 316 stainless steel, fused silica, Tygon
• Operating temperature: 4 to 40 °C (39 to 104 °F)
• Operating humidity: 20% to <95%, noncondensing
  
  

Specifications:

• Number of solvents: Up to four: in any combination of two: Al or A2 and Bl or B2
• Solvent storage: Solvents Tray accommodates up to four chromatographic solvents, two Sample Manager wash solvents and one Binary Solvent Manager seal wash solvent
• Solvent conditioning: Vacuum degassing (Six-channel): one channel per solvent, and two channels for Sample Manager wash solvents
• Operating flow rate range: 010 to 2.000 mL/min, in 0.001 mL increments
• Compressibility compensation: Automatic and continuous
• Effective system delay volume: <120 ulL, independent of system backpressure (with standard 50-ilL mixer installed)
• Plunger seal wash: Integral, active, programmable
• Gradient profiles: Eleven gradient curves [including linear, step (2), concave (4), and convex (4)]
• Wet prime: Automatic
• Maximum operating pressure: 15,000 psi up to 1 mL/min, 9,000 psi up to 2 mL/min per pump, not more than 15,000 psi total
• Composition accuracy:
  • ±0.5% absolute (full scale) from 5 to 95% of flow rates
  • from 0.5 to 2.0 mLlmin (Contact Waters for conditions used)
• Composition precision: 0.15% RSO or ±0.04 min SO, whichever is greater, based on retention time
• Flow precision: 0.075% RSO or ±0.02 min SO, six replicates, based on retention time or volumetric measures (0.500 to 2.000 ml/min)
• Flow accuracy: ± 1.0% at 0.5 mL/min with degassed methanol, per Waters AQT/SystemsQT protocol
• Primary wetted materials: 316 stainless steel, UHMWPE, sapphire, ruby, FEP, PTFE, ETFE, diamond-like coating, PEEK and PEEK alloys, titanium alloys
• Unattended operation: Leak sensors, full diagnostic data captured through console software

The Waters ACQUITY UPLC H-Class System delivers the flexibility of quaternary solvent blending with the advanced performance of UPLC® separations. The system's holistic design is targeted for routine analysis and method development use and is perfectly suited for running both HPLC and UPLC applications while still realizing the improved resolution and sensitivity of UPLC separations.

Includes:

• TUV Detector (PDA Detector $1000 additional)

• Sample Manager - FTN

• Column Manager to control up to 4 Columns
• Quaternary Solvent Manager
  • 15,000 psi up to 1.0 mL/min,
  • 9000 psi up to 2.0 mL/min
• Computer Loaded with Empower

Specifications:

• Dwell volume (total system): <400 ~L (includes standard 100 ~L mixer)
• Integrated leak management: Leak sensors, as standard, and safe leak handling
• Quantum synchronization: Injection synchronization between pump and sample manager enhances retention time reproducibility
• Set table flow rate range:
  • 01 0 to 2.000 mLlmin, in 0.001 mL increments (firmware version 1.5x and earlier)
  • 010 to 2.200 mL/min, in 0.001 mL increments (firmware version 1.60)
  • 001 to 2.200 mLlmin in 0.001 mL increments (firmware version 1.65 and later)
• Maximum operating pressure:
  • 15,000 psi up to 1.0 mL/min, 9000 psi up to 2.0 mL/min (firmware version l.5x and earlier)
  • 15,000 psi up to 1.0 mL/min, 7800 psi up to 2.2 mL/min (firmware version 1.6x and later)
• pH range: 2 to 12
• Unattended operation: Leak sensors and safe leak handling, full 96-hour diagnostic data display through console software
• Cycle time: <30s Inject to Inject